• The Korean Journal of Zoology
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• v.35 no.3
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• pp.322-331
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• 1992

To investigate the svnthyesis of muscle proteins during differentiation of chicken myoblast, cvtosolic and membrane fractions were used for both sodium dodecvl sulfate polvcrylamide gel eBectrophoresis and two-dimensional gel electrophoresis. An extensive cell fusion was observed in 4 day culture. In the protein pattern of the cvtosolic fraction from SDS-PAGE. several protein bands including 250 kDa and 46 kDa showed remarkable changes during culture. the protein of 46 kDa was the most prominent one ann its optical density was the highest in 5 day culture (OD = 1.30). In the membrane fraction, band of 19.8 kDa showed the highest absorbance with 0.93 OD at 12 hr after initial plating and decreased gradually thereafter to 0.23 in 5 nay culture. From the results of two-dimensional gel electrophoresis of cytosolic fraction, the 46 kDa spot was observed as ko separated forms from culture 2 nary culture, and the sixte of this spot was the largest in 5 nay culture. In the pattern of membrane protein, the extensive appearance of newiv synthesized Proteins was found in a naut culture, but no Prominent spot was observed throughout culture. From the results of the present clay, we found that, during myoblast differentiation, the most prominent proteins were bands of 46 kDa and 19.8 kDa in cvtosolic and membrane fraction, respectively, and the appearance of new proteins was initiated at 48 hr after initial plating, and the 46 kDa protein was predominant in the cytoplasm of late culture in which extensive cell fusion was observed.

• Proceedings of the Korea Society of Poultry Science Conference
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• 2003.11a
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• pp.87-88
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• 2003

Saccharomyces cerevisiae(SC)가 육계의 성장능력과 육질 개선효과가 있는지 알아보기 위하여 2회의 사양시험을 수행하였다. 실험1에서는 갓 부화한 160수의 수컷 병아리를 두 처리로 나눈 후에, 각각 0 ％ 와 3.0 ％의 SC를 함유한 사료를 5주간 급여하였다. 실험2에서는 240수의 수컷 병아리를 4처리로 나눈 후, 각각 0, 0.3, 1.0 및 3.0 ％의 SC를 역시 5주간 급여하였다. 실험1에서는 SC 급여구는 무급여구에 비하여 체중이 무거운 경향을 보였으나 유의성은 없었다 (P＞0.05), 실험2에서는 SC 급여수준이 증가함에 따라 체중과 사료섭취량이 유의하게 증가하였다(linear P＜0.05). 실험1에서 SC 첨가는 다리근육(drumstick)의 전단력을 유의하게 감소시켰으나, 삶은 다리근육 에서는 이러한 효과가 관찰되지 않았다. 실험2에서는 흉근과 대퇴근 및 다리근육 및 피부의 산화 안정성은 SC 첨가에 의하여 유의하게 개선되었다.

• Korean Journal of Food Science and Technology
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• v.28 no.3
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• pp.566-572
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• 1996

The relationship between the myofibrillar fragmentation and zeugmatin during post-mortem aging was in vestigated by indirect immunofluorescence method using antizeugmatin Antiserum as a measure of meat tenderization. The antizeugmatin antiserum was prepared using bands separated by SDS-PAGE and reacted specifically with zeugmatin, showing no cross-reactivity with the other myofibrillar proteins. By the indirect immunofluorescence method, this antiserum stained the fresh myofibrillar However, the fluorescence intensity decreased with post-mortem time and almost disappeared within 24 hr of storage, in parallel with the myofibrillar fragmentation. It was therefore concluded that zeugmatin can be conveniently used as a measure of meat tenderization during post-mortem aging by immunoflurescence method.

• Journal of Chest Surgery
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• v.30 no.12
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• pp.1247-1250
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• 1997

A 13-year-old boy presented with anterior chest wall depression and dyspnea on exertion(NYHA II). He underwent Ravitch operation for pectus excavatum 7 years ago. A preoperative echocardiographic study revealed secundum atrial septal defect. He had no other abnormality of laboratory test, except FVC and FEVI were decreased into 2.03 L(7 %) and 1.82 L(71 %). He underwent repair of secondary anterior chest wall deformity and secundum atrial septal defect. We used unique method, raising sternum at right angle to secure good operative field for open heart surgery. Acute respiratory insufficiency was developed on postoperative day 1. Mechanical ventilation was applied which could be weaned on postoperative day 6 and thereafter hospital course was uneventful without any other sequale. He was discharged on postoperative day 19.

• Korean Journal of Head & Neck Oncology
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• v.27 no.1
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• pp.12-16
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• 2011

Background and Objectives : The pectoralis major myocutaneous flap(PMMCF) has been considered to be the "workhorse" of pedicled flaps in head and neck reconstruction. Even with the worldwide use of free flaps, the PMMCF is still considered the mainstay in head and neck reconstruction. The aim of the study is to evaluate the application and reliability of the PMMCF in selected cases of head and neck complication. Materials and Methods : We conducted a retrospective review of 14 patients who underwent the surgical reconstruction using the PMMCF due to the complications after head and neck ablative surgery between 1997 and 2007. Outcome measures included the indications of PMMCF, complications and post-operative functional result. Results : PMMCFs were used to reconstruct defects in the following series; wound dehiscence(7 patients), flap failure(4 patients), pharyngocutaneous fistula(3 patients). Flap survival was 100 percent and mean flap size was $67.2cm^2$. Five patients had complications such as pharyngocutaneous fistula, marginal necrosis, carotid blowout. Conclusions : The PMMCF is a safe and convenient method for reconstruction of the surgical complications after resection of advanced tumors and can be still used as a salvage procedure after free flaps failure.

• Korean Journal of Poultry Science
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• v.31 no.1
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• pp.1-7
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• 2004

An experiment was conducted to investigate whether Saccharomyces cerevisiae(S. cerevisiae) could improve the growth performance and meat quality of broiler chicks. Day old 160 male broiler chicks were fed one of the two experimental diets without (0.0 %) or with S. cerevisiae (3.0 %) for five wks. Each treatment consisted of eight cages with 10 chicks per cage. Feed and water were provided ad libitum. Although not significant, BW gains of S. cerevisiae fed chicks tended to increase during 4∼5 wk of age. The addition of S. cerevisiae into the control diet significantly lowered the shear force in raw drumstick meat (P＜0.05). After 10 d of incubation, significantly lower levels of oxidation products were found (P＜0.05) in drumstick meats and skin samples from broiler chicks fed diets enriched with S. cerevisiae compared to those of the control group, while in breast meats the significant difference was monitored after 6 d of incubation. It is concluded that dietary S. cerevisiae could improve the tenderness and oxidative stability of broiler meats.

• Journal of the Korean Arthroscopy Society
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• v.13 no.2
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• pp.174-178
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• 2009

Purpose: We introduce arthroscopic assisted core decompression for humeral head osteonecrosis. Operative technique: After diagnostic shoulder arthroscopy is performed using posterior and anterior portal, we make a 2 cm lateral skin incision approximately 3 cm distal to 1/3 of lateral margin of the acromion and place short gray cannula to prevent adjacent soft tissue injury when insert guide pin. Under C-arm fluoroscopic and arthroscopic guidance, 3 to 4 guide pins are inserted toward the necrotic area. Then we perform drilling using 7.0 mm reamer. Conclusion: We believe this technique is a less invasive approach and avoids the complications comparing to deltopectoral approach. Arthroscopic assisted core decompression is an effective procedure in management of early stage of the humeral head osteonecoris.

• Biomedical Science Letters
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• v.6 no.4
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• pp.269-279
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• 2000

This present study was investigated to elucidate degenerative changes according to the change of habitual environment on the myocytes of doves by restricting them from flight that is instinct behavior of this animal and strong exercise. To restrict doves from flight, they were confined in the cage (1 $m^3$) for 2 months. After this period, the myocardium of the experimental group was compared to that of wild doves in the ultrastructural and cytochemical ways. In addition, stereological changes were also examined. The results were as followings: 1. The body weight of the confined experimental groups was higher than that of the wild doves, but the ratios of the pectoral muscle/body weight (p<0.05) and the heart/body weight were lower. 2. At the ultrastructural level, the myocardium of confined doves appeared as wavy fibers in the smaller area than in the myocardium of wild doves. Also, the length of sarcomeres was longer in the confined doves. The number of sarcoplasmic reticulum and capillary was smaller in the myocardium of confined doves. 3. Cytochemical examinations showed that the activities of cytochrome oxidase were lowered in the confined doves. 4. Stereological analysis revealed that the density of myofibrils was greater in the confined doves. In contrast the volume density of sarcoplasmic reticulum (p<0.05) and the surface density of mitochondrial inner membrane (p<0.05) was lower in the confined doves, while the numerical density of mitochondrial inner membrane was higher (p<0.05). These results suggest that even the short period of restricted exercise can induce negative effects on the functions of myocytes of doves that are adapted for the strong exercise such as flight. Therefore, the maintenance of prolonged exercise seems to be one of the important factors that are critical to retain the functions of myocardium.

• Korean Journal of Poultry Science
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• v.31 no.4
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• pp.273-281
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• 2004

A feeding trial was conducted with Euglena strains grown under different media. The effect of supplementation of Euglena on the performance, nutrient availability and fatty acid composition of breast muscle was studied. In experiment I, two hundred ten hatched broiler chicks (Ross) were assigned to seven dietary treatments for 5 weeks. Each treatment consisted of 3 replications with 10 birds each. Control diet was formulated to have $22\%$ CP and 3,150 kcal ME/kg for starter diet, $19\%$ CP and 3,200 kcal ME/kg for finisher diet. Euglena gracilis Z. (EG) was added to control diet at the plevel of 0.25, 0.5, $1.0\%$ and Euglena gracilis Z. bleached and DHA enriched (EGBD; a strain mutated by streptomycin and cultivated in DHA enriched medium) at the level of 0.5, 1.0, $2.0\%$ in the diet. In experiment 2, two hundred fifty hatched broiler chicks (Ross) were assigned to five dietary treatments: T1; Control, T2; T1 + Euglena gracilis Z. DHA enriched (EGD; cultivated in DHA enriched medium) $0.5\%$, T3; T1 + EGD $1.0\%$, T4; T1 + EGBD $0.5\%$, T5; T1 + EGBD $1.0\%$. The weight gain and feed consumption were measured weekly. Fatty acid composition of breast muscle was determined. In experiments I and 2, Euglena supplementation had no significant effects on weight gain, feed intake and feed conversion ratio. In experiment 1, EGBD treatments significantly increased DHA concentration but decreased concentration of linoleic acid and arachidonic acid in breast muscle. EGBD 2% treatment showed the highest DHA concentration (14.27%) which is 3.9 times of that of the control ($3.66\%$). In experiment 2, $1.0\%$ EGBD treatment showed highest EPA, lignoceric acid and DHA level in breast muscle (P<0.05). Also, EGD treatments significantly increased DHA and EPA concentration. It was concluded that EGBD and EGD can be supplemented to broiler diet to produce DHA enriched broiler meat.