• Journal of The Korean Society of Grassland and Forage Science
• /
• v.33 no.2
• /
• pp.131-138
• /
• 2013

The present study was conducted with three ruminally cannulated non-lactating Holstein cattle in a $3{\times}3$ Latin square design to determine the fermentation characteristics and effective degradability (ED) in the rumen, as well as the whole tract digestibility of whole crop silage based total mixed ration (TMR) in comparison with conventional separate feeding of concentrate and rice straw for late fattening Hanwoo steers. The cattle in each group were fed separate feeding of concentrate and rice straw (control), whole crop barley silage based TMR (BS-TMR) or whole crop rye silage based TMR (RS-TMR). The ruminal fermentation characteristics such as pH, ammonia-N concentration and total volatile fatty acid were not affected by the experimental diet. The molar portion of acetate ($C_2$) was lowest in rumen fluid 1 h after feeding when cattle were fed BS-TMR (p<0.033). Molar proportions of propionate ($C_3$), butyrate and $C_2/C_3$ were not influenced by the experimental diet. There were no differences in effective degradability or whole tract digestibility of feed components among any of the experimental diets. The data obtained from the metabolism trial indicate that the feeding value of TMR with BS or RS is equal to that of a conventional separate feeding of concentrate and rice straw.

• Korean Journal of Environmental Agriculture
• /
• v.26 no.2
• /
• pp.179-185
• /
• 2007

A new technology that utilizes a microbial fuel cell (MFC) has been developed to generate electricity directly from the oxidation of organic matters such as carbohydrates or complex organics in wastewater. Fermentation of these organic matters results in production of volatile fatty acids (VFAs), alcohols, $CO_2$ and $H_2$. We investigated the electricity-producing potential of the VFAs and actual food processing wastewater using a two-chambered MFC. The electrons produced by acetate degradation were proportional to acetate concentration in the medium. Acetate concentration and generated power were linearly correlated at a low range or acetate concentration (< 8 mg/L), but at above 8 mg/L of acetate the power produced was maintained at 0.1 mW. When butyrate was added to the anode acclimated to acetate, there was a lag period of 30 hr for electricity generation. However, when propionate was added to the same anode bottle, lag periods were not existed. The wastewater from baby food processing generated the maximum power density of $81{\pm}7\;mW/m^2$ of electricity and exhibited the Coulombic efficiencies of 27.1% and 40.5% based on TCOD and SCOD, respectively. Sugars in the food processing wastewater were reduced within 50 h from 230 mg/L < 30 mg/L.

• Korean Journal of Organic Agriculture
• /
• v.22 no.4
• /
• pp.801-813
• /
• 2014

A study was conducted to improve the biological activity of two medicinal plants, Eucommia ulmoides Oliv. and Glycyrrhiza uralensis, by fermentation. The biological activity was assessed by determining antibacterial, antioxidant and antimethanogenic properties. Fermentation was achieved by adding the plant materials in MRS broth at 10% (w/v) and different starter cultures at 1% (v/v). Condition for fermentation were incubation temperature of $30^{\circ}C$ and agitation at 150 rpm for 48 h. Six starter cultures, Weissella confusa NJ28 (Genbank accession number KJ914897), Weissella cibaria NJ33 (Genbank accession number KJ914898), Lactobacillus curvatus NJ40 (Genbank accession number KJ914899), Lactobacillus brevis NJ42 (Genbank accession number KJ914900), Lactobacillus plantarum NJ45 (Genbank accession number KJ914901) and Lactobacillus sakei NJ48 (Genbank accession number KJ914902) were used. Antibacterial activity was observed in L. curvatus NJ40 and L. plantarum NJ45 only as opposed to other treatments, including the non-fermented groups, which showed no antibacterial activity. Both plants showed antioxidant activity, although E. ulmoides Oliv. had lower activity than G. uralensis. However, fermentation by all strains significantly improved (p<0.05), antioxidant activity in both plants compared to non-fermented treatment. Six treatments were based on antibacterial activity results, selected for in vitro rumen fermentation; 1) non-fermented E. ulmoides, 2) fermented E. ulmoides NJ40, 3) fermented E. ulmoides NJ45, 4) non-fermented G. uralensis, 5) fermented G. uralensis NJ40, 6) fermented G. uralensis NJ45. A negative control was also added, making a total of 7 treatments for the in vitro experiment. Medicinal plant-based treatments significantly improved (p<0.05) total volatile fatty acid (VFA) concentration. Significant methane reduction per mol of VFA were observed in G. uralensis (p<0.05). Based on the present study, fermentation improves the biological activity of E. ulmoides Oliv. and G. uralensis. Fermented G. uralensis could also be applied as an enteric methane mitigating agent in ruminant animals.

• Korean Journal of Soil Science and Fertilizer
• /
• v.22 no.4
• /
• pp.323-328
• /
• 1989

This study was conducted to find out the behavior of anaerobic microorganisms and anaerobic decomposition products of rice straw in the strict anaerobic condition. The number of methanogenic bacteria were more isolated than cellulolytic bacteria from the digester decaying rice straw during the entire incubation time. The activity of anaerobic microorganisms, such as methanogens and cellulolytics, were high the early incubation time in the treatment of rice straw with urea, but without urea was low at that time and increased moderately after 10 days incubation. Volatile fatty acid as intermediate anaerobic decomposition products had a longer retention time and higher accumulation rate in the treatment of rice straw without urea than with urea, and predominant fatty acid was propionic acid. Gas generation rate as final products were very intimate relationship with the activity of methanogenic bacteria. Average Eh value was -250mV during the incubation time and $CH_4$ : $CO_2$ percent ratio was about 60~65 : 35~40 in this Eh value. Decomposition rate of rice straw calculated from $CH_4$ and $CO_2$ gas wars 45.6% for 50 days in the treatment of rice straw with urea, and 36.8% without urea.

• Journal of agriculture & life science
• /
• v.51 no.4
• /
• pp.97-109
• /
• 2017

This study was conducted to investigate the effects of anti-inflammatory plant extracts on the in vitro rumen fermentation characteristics and methane emission. Anti-inflammatory plant extracts from Morus bombycis Koidz, Mallotus japonicus L., Morus alba L., Paulownia coreana Uyeki, Isodon japonicus Hara and Ginkgo biloba L. were used in the study. The ruminal fluid(5 mL), McDougall buffer(10 mL), timothy as a substrate(0.3 g) and each anti-inflammatory plant extract(5% of substrate) were dispensed anaerobically into 50mL serum bottle. The mixtures were incubated for 3, 9, 12, 24, 48 and 72h at $39^{\circ}C$ without shaking. Supplementation of the anti-inflammatory plant extracts did not effects characteristics(pH, digestibility of dry matter, glucose concentration, ammonia concentration, protein concentration, VFA) on rumen fermentation. Total gas was showed a different pattern depending on treatments. Carbon dioxide was significantly(p<0.05) higher in Morus alba and Isodon japonicus than in control at 48h. Methane was significantly(p<0.05) lower in treatment than in control at initial fermentation. However the more incubation time was increased, the more methane emission was higher in treatment than in control. The concentrations of polyphenol and flavonoid were higher in Ginkgo biloba. In conclusion, supplementation of the anti-inflammatory plant extracts did not effect on rumen fermentation and methane emission was decreased in initial fermentation.

• Journal of Animal Science and Technology
• /
• v.48 no.3
• /
• pp.393-400
• /
• 2006

The present study was conducted to evaluate feeding value of brown rice in Korean native beef steers (Hanwoo) fed concentrates and orchardgrass hay (50:50 on a wet basis) as a basal diet. Either 50% or 100% of corn in concentrates was substituted for brown rice, and ruminal fermentation characteristics, in situ degradability of feeds and in vivo digestibility of concentrate were investigated. The digestibility and nutritive values of corn and brown rice feeds were similar, whereas rumen degradability of brown rice was higher than that of corn. Compared with corn-based diets, the brown rice substitution decreased (P<0.05) ruminal NH3-N concentration, but did not affect rumen pH. Total volatile fatty acid content in ruminal digesta for the corn based diet was higher than those for the brown rice-contained diets. The brown rice substitution increased (P<0.05) the ratio of ruminal acetic acid to propionic acid (A/P) compared with corn-contained diets.Present results indicate that corn might nutritionally be substituted for brown rice as cattle feeds. In addition, we expect that relatively high A/P with brown rice may produce affirmative effects in rumen physiological aspect, particularly under the grain based feeding system for beef cattle in Korea.

• Journal of Life Science
• /
• v.30 no.9
• /
• pp.743-748
• /
• 2020

Sulfate is a reductant that competes for electrons and may lower CH₄ production in the rumen. This study was designed to evaluate the beneficial effect of detoxified sulfur powder supplementation on in vitro rumen fermentation and methane mitigation. A ruminally cannulated Holstein Friesian cow was used as a rumen fluid source, and commercial pelleted concentrate was used as a substrate at 1 g dry matter. Treatments included the addition of detoxified sulfur powder at the rate of 0% (Control), 0.2% (T1), 0.4% (T2), 0.6% (T3), 0.8% (T4), and 1.0% (T5) as dry matter (DM) basis. The pH, total gas (TG), methane (CH₄) production, DM digestibility, organic matter (OM) digestibility, and volatile fatty acids (VFA) production were analyzed after 12 hr of incubation. The results showed that CH₄ production was significantly lowest in T1 (13.78 ml) but highest in the control (20.16 ml). Insignificantly higher total VFA was observed in control and T1 (64.99 and 64.28 mM, respectively) compared to other treatments after 12 hr of incubation. After 12 hr of incubation, the significantly lowest acetate:propionate was observed in T1 (1.90) while the highest was observed in T4 (2.44). However, no significant differences were recorded for pH, TG, DM digestibility, OM digestibility, acetate, propionate, and butyrate between the control and T1. Total number of bacterial DNA copies was significantly lower in the treatment group than the control. Therefore, it can be concluded from this study that detoxified sulfur at 0.2% inclusion level is optimal for production performance and ruminal CH₄ mitigation.

• Journal of the Korea Organic Resources Recycling Association
• /
• v.25 no.3
• /
• pp.91-98
• /
• 2017

The purpose of this study is to provide a design and operation technical guideline for meeting the appropriate design criteria to bio-gasification facilities treating organic wastes. 9 anaerobic digestion facilities which is normally operated during the field survey and 14 livestock manure farms were selected for precision investigation. the physicochemical analysis was performed on the moisture and organic contents, nutrients composition (carbohydrate, fat, protein), volatile fatty acids (VFAs), and nitrogen, etc. Volatile solids (VS) of organic wastes brought into the bio-gasification facilities were 2.81 % (animal manure only) and 5.92 % (animal manure+food waste), respectively. Total solids (TS) reults of samples from livestock farms were 5.6 % in piglets and 11~13 % in other kinds of breeding pigs. The actual methane yield based on nutrients contents was estimated to $0.36Sm^3CH_4/kgVS$ which is equivalent to 72 % of theoretical methane yield value. The optimum mixing ratio depending on the effect of the combined bio-gasification was obtained through the continuous stirred-tank reactor (CSTR) which is operated at different mixing ratio of swine manure and food waste leachate. The range of swine manure and food waste leachate from 60:40 to 40:60 were adequate to the appropriate conditions of anaerobic digestion; less than 100 gTS/, more than alkalinity of 1 gCaCO3/L, C/N ratio 12.0~30.0, etc.

• Journal of the Korean Society of Food Science and Nutrition
• /
• v.14 no.4
• /
• pp.370-374
• /
• 1985

To investigate the flavor components of sun-dried ray, Raja porasc, the contents of such as free amino acids, nucleotides and their related compounds, total creatinine, betaine, TMAO, fatty adds, and volatile compounds were analysed. The content of total free amino acids was 1773.3mg% on dry basis and the abundant amino acids were taurine, lysine, leucine, alanine, glycine, glutamic acid, proline, and valine in order and these amino acids accounted for 80% of total free amino acids in sun-dried ray. In the nucleotides and their related compounds, hypoxanthine was the most abundant. Ammonia-N was the most abundant, resulting 26.4% of extractive nitrogen which was 1965.6mg% and next free amino acid-N, total creatinine-N, and nucleotide-N in order. In the fatty acid composition of total lipid and neutral lipid, polyenoic acid was abundant holding 42.0%, 45.3% respectively. In phospholipid, the ratio of polyenoic acid and saturated fatty acid were similar to 39% respectively. The predominant fatty acids in total lipid, neutral lipid, and phospholipid were $C_{16:0},\;C_{22:6},\;C_{18:1},\;C_{18:0},\;and\;C_{20:4}$. In the volatile compounds, isocaproic acid, caproic acid, isobutyric acid, and butyric acid were the major portion of the 8 kinds of volatile fatty acids detected and 2-butanone, pentanal, and 2-methylpropanal were the major portion of the 8 kinds of volatile carbonyl compounds detected and also trimethylamine was the major portion of 2 kinds of volatile amines detected. It was presumed from the results that the reciprocal action of taste compounds such as free amino acids, nucleotides and their related compounds total creatinine, betaine, and TMAO and volatile compounds such as volatile carbonyl compounds, volatile fatty acids, volatile amines, and ammonia played an important role for the characteristics of flavor of sun-dried ray.

• Journal of Animal Science and Technology
• /
• v.48 no.1
• /
• pp.101-106
• /
• 2006

In this study, changes of ammonia, alcohol and volatile fatty acid(VFA) concentrations or pH in in vitro ruminal fluid were studied to determine the effects of alcoholic feeds on in vitro ruminal fermentation characteristics. To formulate the alcoholic feeds, alcohol was added to commercial formulated feed at the levels of 1, 3, and 5 %. Experiments were done with four treatment groups, control(commercial feed), AF-1(commercial feed+1% alcohol), AF-3(commercial feed+3% alcohol), and AF-5(commercial feed+5% alcohol). Ammonia concentrations of AF-1 and AF-5 were significantly lower than that of control for the 12h incubation(p<0.05). Ruminal alcohol concentration was increased with the addition level of alcohol increased(p<0.05). TVFA concentrations of AF-1, AF-3 and AF-5 were significantly higher than those of control at 12h(p<0.05). Significant decrease of molar percentage of acetate was observed in control from 8 to 12h incubation, but molar percentage of acetate for AF-1, AF-3 and AF-5 was constant. Molar percentage of propionate was increased in control compared with AF-1, AF-3 and AF-5 from 8 to 12h incubation(p<0.05). Molar percentages of butyrate and valerate were higher in AF-1, AF-3 and AF-5 than in control(p<0.05). Molar percentage of caproate for AF-1, AF-3 and AF-5 was 0.05, 0.58 and 0.47M% at 8h, respectively, but that was not detected for control. Present results may indicate that the alcoholic feeds show positive effects on in vitro ruminal ammonia, alcohol and VFA concentrations or pH. Furthermore, the results of this study implies that the addition level of 5% could be more effective to ruminal fermentation than other addition levels.