Browse > Article
http://dx.doi.org/10.5352/JLS.2020.30.9.743

Effects of Detoxified Sulfur as a Feed Supplement on in Vitro Rumen Fermentation and Methane Mitigation  

Kim, Seon-Ho (Ruminant Nutrition and Anaerobe Laboratory, Department of Animal Science and Technology, Sunchon National University)
Islam, Mahfuzul (Ruminant Nutrition and Anaerobe Laboratory, Department of Animal Science and Technology, Sunchon National University)
Biswas, Ashraf Ali (Ruminant Nutrition and Anaerobe Laboratory, Department of Animal Science and Technology, Sunchon National University)
Cho, Kwang-Keun (Department of Animal Resources Technology, Gyeongnam National University of Science and Technology)
Lee, Sang-Suk (Ruminant Nutrition and Anaerobe Laboratory, Department of Animal Science and Technology, Sunchon National University)
Publication Information
Journal of Life Science / v.30, no.9, 2020 , pp. 743-748 More about this Journal
Abstract
Sulfate is a reductant that competes for electrons and may lower CH4 production in the rumen. This study was designed to evaluate the beneficial effect of detoxified sulfur powder supplementation on in vitro rumen fermentation and methane mitigation. A ruminally cannulated Holstein Friesian cow was used as a rumen fluid source, and commercial pelleted concentrate was used as a substrate at 1 g dry matter. Treatments included the addition of detoxified sulfur powder at the rate of 0% (Control), 0.2% (T1), 0.4% (T2), 0.6% (T3), 0.8% (T4), and 1.0% (T5) as dry matter (DM) basis. The pH, total gas (TG), methane (CH4) production, DM digestibility, organic matter (OM) digestibility, and volatile fatty acids (VFA) production were analyzed after 12 hr of incubation. The results showed that CH4 production was significantly lowest in T1 (13.78 ml) but highest in the control (20.16 ml). Insignificantly higher total VFA was observed in control and T1 (64.99 and 64.28 mM, respectively) compared to other treatments after 12 hr of incubation. After 12 hr of incubation, the significantly lowest acetate:propionate was observed in T1 (1.90) while the highest was observed in T4 (2.44). However, no significant differences were recorded for pH, TG, DM digestibility, OM digestibility, acetate, propionate, and butyrate between the control and T1. Total number of bacterial DNA copies was significantly lower in the treatment group than the control. Therefore, it can be concluded from this study that detoxified sulfur at 0.2% inclusion level is optimal for production performance and ruminal CH4 mitigation.
Keywords
Detoxified sulfur; in vitro; methane mitigation; ruminant;
Citations & Related Records
연도 인용수 순위
  • Reference
1 Asanuma, N. and Hino, T. 2000. Activity and properties of fumarate reductase in ruminal bacteria. Gen. App. Microbiol. 46, 119-125.   DOI
2 Asanuma, N., Iwamoto, M. and Hino, T. 1999. Effect of the addition of fumarate on methane production by ruminal microorganisms in vitro. J. Dairy Sci. 82, 780-787.   DOI
3 Beauchemin, A. K. and McGinn, S. M. 2005. Methane emission from feedlot cattle fed barley or corn diets. J. Anim. Sci. 83, 653-661.   DOI
4 Broucek, J. 2014. Production of methane emissions from ruminant husbandry: a review. J. Env. Prot. 5, 1482-1493.   DOI
5 Christophersen, C. T. 2007. Grain and artificial stimulation of the rumen change the abundance and diversity of methanogens and their association with ciliates, school of Animal Biology, University of Western Australia, Perth.
6 Hino, T., Mukunoki, H., Imanishi, K. and Miyazaki, K. 1992. Necessity of ready electron disposal and interspecies hydrogen transfer for the utilization of ethanol by rumen bacteria. Asian Australas. J. Anim. Sci. 5, 511-517.   DOI
7 Forster, P., Ramaswamy, V., Artaxo, P., Berntsen, T., Betts, R., Fahey, D. W., Haywood, J., Lean, J., Lowe, D. C., Myhre, G., Nganga, J., Prinn, R., Raga, G. M. S. and Van Dorland, R. 2007. Changes in atmospheric constituents and in radiative forcing. In: The physical science basis. Contribution of working group I to the fourth assessment report of the intergovernmental panel on climate change (Ed. S. Solomon et al.). Cambridge University Press, Cambridge, U.K.
8 Han, S., Kim, S. and Shin, H. 2005. UASB treatment of wastewater with VFA and alcohol generated during hydrogen fermentation of food waste. Process Biochem. 40, 2897-2905.   DOI
9 Hattori, K. and Matsui, H. 2008. Diversity of fumarate reducing bacteria in the bovine rumen revealed by culture dependent and independent approaches. Anaerobe 14, 87-93.   DOI
10 In, D., Yu, D., Park, C. and Park, J. 2012. Physiochemical Analysis, toxicity test and anti-bacterial effect of practically detoxified sulfur. Kor. J. Vet. Serv. 35, 197-205.   DOI
11 Isa, Z., Grusenmeyer, S. and Verstraete, W. 1986. Sulfate reduction relative to methane production in high-rate anaerobic digestion: microbiological aspects. Appl. Environ. Microbiol. 51, 580-587.   DOI
12 Johnson, K. A. and Johnson, D. E. 1995. Methane emissions from cattle. J. Anim. Sci. 73, 2483-2492.   DOI
13 Quinn, M., May, M., Hales, K., DiLorenzo, N., Leibovich, J., Smith, D. and Galyean, M. 2009. Effects of ionophores and antibiotics on in vitro hydrogen sulfide production, dry matter disappearance, and total gas production in cultures with a steam-flaked corn-based substrate with or without added sulfur. J. Anim. Sci. 87, 1705-1713.   DOI
14 Kung, L., Bracht, J. and Tavares, J. 2000. Effects of various compounds on in vitro ruminal fermentation and production of sulfide. Anim. Feed Sci. Technol. 84, 69-81.   DOI
15 NRC, 2005. Mineral tolerance of animals. 2nd ed. Natl. Acad. Press, Washington, DC.
16 Orskov, E. and McDonald, I. 1979. The estimation of protein degradability in the rumen from incubation measurements weighted according to rate of passage. J. Agric. Sci. 92, 499-503.   DOI
17 Pakmaluek, P., Wachirapakorn, C., Saenjan, P. and Yuangklang, C. 2011. Effect of sulfur-containing compounds on methane production by in vitro gas production. In: SAADC 2011 strategies and challenges for sustainable animal agriculture-crop systems, Volume III: full papers. Proceedings of the 3rd International Conference on sustainable animal agriculture for developing countries, Nakhon Ratchasima, Thailand, 26-29 July, 2011, Suranaree University of Technology. pp. 613-619.
18 Promkot, C., Wanapat, M., Wachirapakorn, C. and Navanukraw, C. 2007. Influence of sulfur on fresh cassava foliage and cassava hay incubated in rumen fluid of beef cattle. Asian Australas. J. Anim. Sci. 20, 1424.   DOI
19 Rahman, M. A., Sawiress, F. A. and Abd El-Aty, A. M. 2010. Effect of sodium lauryl sulfate-fumaric acid coupled addition on the in vitro rumen fermentation with special regard to methanogenesis. Vet. Med. Int. 2010, 858474.   DOI
20 Riegel, E. and Kent, J. 2007. Kent and Riegel's handbook of industrial chemistry and biotechnology 1. New York: Springer. p. 1171.
21 SAS, 2004. SAS/STAT. Statistical analysis systems for windows. Release 9.1, p. 423. SAS Institute Inc., Cary, N.C., USA.
22 Ungerfeld, E. and Kohn, R. 2006. The role of thermodynamics in the control of ruminal fermentation. Ruminant physiology: Digestion, metabolism and impact of nutrition on gene expression, Immunology and Stress. pp: 55-85.
23 Smith, D., DiLorenzo, N., Leibovich, J., May, M., Quinn, M., Homm, J. and Galyean, M. 2010. Effects of sulfur and monensin concentrations on in vitro dry matter disappearance, Hydrogen sulfide production, and volatile fatty acid concentrations in batch culture ruminal fermentations. J. Anim. Sci. 88, 1503-1512.   DOI
24 Spears, J., Ely, D. and Bush, L. 1978. Influence of supplemental sulfur on and microbial fermentation of Kentucky 31 Tall Fescue. J. Anim. Sci. 47, 552-560.   DOI
25 Tabaru, H., Kadota, E., Yamada, H., Sasaki, N. and Takeuchi, A. 1988. Determination of volatile fatty acids and lactic acid in bovine plasma and ruminal fluid by high performance liquid chromatography. Jpn. J. Vet. Sci. 50, 1124-1126.   DOI
26 Van Zijderveld, S., Gerrits, W., Apajalahti, J., Newbold, J., Dijkstra, J., Leng, R. and Perdok, H. 2010. Nitrate and sulfate: Effective alternative hydrogen sinks for mitigation of ruminal methane production in sheep. J. Dairy Sci. 93, 5856-5866.   DOI
27 Zafarian, R. and Manafi, M. 2013. Effect of garlic powder on methane production, rumen fermentation and milk production of buffaloes. Annu. Rev. Res. Biol. 3, 1013-1019.
28 Zinn, R. A., Alvarez, E., Mendez, M., Montano, M., Ramirez, E. and Shen, Y. 1997. Influence of dietary sulfur level on growth performance and digestive function in feedlot cattle. J. Anim. Sci. 75, 1723-1728.   DOI