• Proceedings of the Plant Resources Society of Korea Conference
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• 2010.10a
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• pp.19-19
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• 2010

황칠나무(Dendropanax morbifera Lev.)는 두릅나무과(Araliaceae)에 속하며 학명에서 뜻하는 바와 같이 목본 (Dendro), 전능약(Panax)이라는 의미가 있고 나무인삼이라 불리기도 하며 줄기에 상처를 내면 노란액이 나온다고 해서 황칠나무(D. morbifera)라는 이름이 붙여졌다. 두릅나무과는 우리나라에서 최고의 약재들로 손꼽히는 인삼(Panax ginseng), 가시오갈피(Eleutherococcus senticosus) 등의 약용식물을 포함하고 있어서 황칠나무는 황칠수지액 이외에 약용식물로서의 무한한 개발 가능성을 내포하고 있다. 따라서 본 실험은 황칠나무의 기내 부정근 유도 및 증식조건의 확립을 목적으로 수행되었다. 우선 황칠나무의 기내 발아체로부터 부위(잎, 줄기, 뿌리)를 달리하여 부정근을 유도한 결과, 잎은 줄기나 뿌리보다 양호한 부정근의 유도를 보였다. 또한 유도된 부정근을 이용하여 옥신의 종류에 따른 부정근 유도율을 조사한 결과 IBA와 NAA는 IAA와 2.4-D보다 높은 유도율을 보였다. IBA의 농도에 따른 유도율과 증식효율은 IBA가 1.0 mg/L 첨가되었을 때 가장 높은 유도 및 증식효율을 보였다. 최적의 액체배지조건을 확인하고자 sucrose의 농도와 염농도를 달리하여 실험한 결과 1/2MS 배지는 MS 배지보다 10%정도 높은 증식율을 보였다. 액체배양 된 황칠나무의 부정근을 각각 1/2MS 배지에 30 g/L sucrose, 3.0 mg/L IBA가 첨가된 5 L volume 생물반응기에 4주 간 배양한 대조구와 2주 후 IBA의 농도를 1.0으로 낮추어 배양한 실험구에서 2주후 IBA의 농도를 낮추어 배양한 실험구에서 대조구보다 약 2배 높은 부정근의 증식량을 보였다. 결국, 황칠나무의 종자발아체를 이용하여 부정근의 유도 및 증식조건에 필요한 기내배양조건을 확립하였고, 플라스크와 생물반응기 배양을 통해 효율적인 실험실 내 증식조건을 확립하였다. 본 실험결과는 향후 황칠나무 천연추출물을 활용한 향장품/식,의약품 소재의 대량확보 차원에서 중요한 가치를 내포하고 있다고 조심스럽게 사료된다.

• Korean Journal of Plant Resources
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• v.17 no.3
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• pp.248-256
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• 2004

Dendropanax morbifera Lev.(Araliaceae), Korean endemic and evergreen small tree is a component of evergreen forest and mainly distributed in sourthern region and islands in Korea. The ecological characteristics of 4 natural stands of D. morbifera.(Sundol, Suak, Hannam, and Sangho populations in Cheju island) were studied. In most of the D. morbifera natural stands, the following tree species appeared predominantly: Castanopsis cuspidata var. sieboldii, Carpinus laxiflora, D. morbifera, Quercus glauca, Quercus myrsinaefolia, Camellia japonica, and Acer pseudo-sieboldianum. Two tree species, such as C. cuspidata var. sieboldii and C. laxiflora appeared in the all investigated stands. D. morbifera occupied 17.2% of the upper story, 12.9% of the middle story, and 10.3% of the lower story, respectively. The distribution patterns by Morisita' s Index showed that D. morbifera was distributed randomly in the three stories. The frequency distribution of DBH D. morbifera species showed reverse J-shaped, therefore it seems to remain as a dominant species.

• KOREAN JOURNAL OF CROP SCIENCE
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• v.48 no.6
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• pp.434-437
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• 2003

This study was carried out to obtain basic information for environment of native area and growth characteristics under various mulching materials in Dendropanox morbifera $\textrm{L}_{EV}$, Dendropax c morbifera was grown wild at Wando area, particularly, distribution frequency was high at 20 to 280 meters above the sea level, and configuration of the ground of native area descended slowly toward the southeast. The soil characteristics of native area was pH 5.3-5.5, 8.8-9.5% in organic matter content and 19.8-22.0% in soil moisture. Growth of Dendropanox morbifera $\textrm{L}_{EV}$, was accelerated by transparent polyethylene film and black polyethylene film mulched.

• Korean Journal of Plant Resources
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• v.14 no.1
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• pp.60-64
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• 2001

This study was carried out to investigate effects of sowing dates and method on major agronomic characteristics of Dendropanax morbifera $L_{EV}$. in southern area of Korea. The number of days from sowing to emergence was recognized liner negative correlation between sowing days. Dendropanax morbifera was sowed at seven different dates(from Dec.20 to Feb.20 at 30 days interval, from Mar, 1 to Apr. 15 at 15 days interval) in pot culture. In sowing date at Mar. 15, germination rate and early growth were good in pot. Dendropanax morbifera was sowed at five different dates(from Dec.20 to Apr.15 at 15 days interval) in field culture. In sowing date at Apr.15, germination rate and early growth were good in field culture.

• Journal of Korean Society of Forest Science
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• v.89 no.1
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• pp.93-104
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• 2000

The ecological characteristics of 10 natural stands of Dendropanax morbifera(Haenam, Wando 1, Wando 2, Bogildo, Kemundo and Hongdo and Sendol, Suoak, Hannam, and Kidowon populations in Cheju island) were studied. In most of the Dendropanax morbifera natural stands, the following tree species appeared predominantly : Camellia japonica, Castanopsis cuspidate var. sieboldii, Castanopsis cuspidate var. thunbergii, Carpinus laxiflora, Quercus acute and Eurya japonica. Especially, Camellia japonica and Eurya japonica appeared in all the stands. Dendropanax morbifera occupied 17.2% of the upper story, 12.9% of the middle story, and 10.3% of the lower story, respectively. The distribution patterns by Morisita's Index showed that Dendropanax morbifera was distributed randomly in all of the three stories, i.e. the upper, the middle and the lower stories. Dendropanax morbifera was positively associated with Camellia japonica, Eurya japonica, Carpinus laxiflora, Cinnamomum japonicum, Ligustrum japonicum, Callicarpa Japonica, Castanopsis cuspidate var. thunbergii, Castanopsis cuspidata var. sieboldii, Daphniphyllum macropodum, Acer palmatum and Euonymus oxyphyllus. This might suggest that the ecological niche is similar to each other. The cluster analysis using percent dissimilarity(PD) based on species composition divided into two groups. One group consisted of the populations located along the south-western coasts. The other group consisted of the populations of Cheju island. This result was agree with that of ordination analysis. From these results, We could find out that the species composition of the populations of Dendrapanax morbifera in Cheju island was quite different from those of other regions.

• Journal of Life Science
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• v.29 no.4
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• pp.455-460
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• 2019

In previous studies, we confirmed the effective antimicrobial activity of fermented Dendropanax morbifera leaf/branch extracts with Lactobacillus plantarum ilchiwhangchil 1785 and Lactobacillus plantarum ilchiwhangchil 2020. In this study, we investigated the hair growth effect of D. morbifera leaf/branch extracts fermented with L. plantarum ilchiwhangchil 1785 and L. plantarum ilchiwhangchil 2020 on human hair dermal papilla cells. The growth rate of human hair dermal papilla cells treated with fermented extracts in the range of 1 to $10{\mu}g/ml$ significantly increased in a concentration-dependent manner, without increasing cell death. Double staining studies showed that the growth of cells treated with fermented D. morbifera leaf/branch extracts was more active than that of control cells. Moreover, the cells treated with the fermented D. morbifera leaf/branch extracts exhibited a 18.84% and 23.31% increase in cell mobility, respectively, as compared with that of the untreated cells. High-performance liquid chromatography (HPLC) was used to determine the active agents responsible for hair growth. The results showed that the content of ${\beta}$-sitosterol, which is known to affect hair growth, increased about 10 times in the fermentation process of D. morbifera leaf/branch extracts. Taken together, the findings confirm that fermented Dendropanax morbifera leaf/branch extracts promote hair growth.

• Korean Journal of Plant Resources
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• v.11 no.1
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• pp.101-105
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• 1998

This study was carried out to find out the method of increasing seed germination rate of Dendropanax morbifera LEV. was drupaceous fruit. The length and width of ovarywas 7.8 to 10.7mm, 6.9 to 9.0mm, respectively, and the shape was elliptical. The ovary had 5 loculi with one speed per loculus , but one seed among 5 weeds was exalbuminous seed. The length and width of seed was 6.3 to 7.4mm, 2.0 to2.mm, respectively, and 1,000 seed weight was 14.3 to 18.0g. Seeding after stroring at $10^{\circ}C$ for 60 to 90 days was germinated to 87% . However, fall seeding directly after harvest was increased to 85% at hot-water treatement ($40^{\circ}C$) for 90 to 120 minute.

• Applied Chemistry for Engineering
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• v.33 no.1
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• pp.44-49
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• 2022

Essential oil was separated from Dendropanax morbiferus (DM) by means of hot water extraction and supercritical extraction, respectively, and the separated essential oil was analyzed qualitatively and quantitatively, and the antioxidant effect of essential oil was investigated. In addition, yields of essential oil and Caryophyllene according to the drying and extraction methods for each part of DM were obtained and compared, respectively. The yield of essential oil was found to be high in the order of bark > leaves > twigs > limb. When the freeze-dried DM leaves were supercritically extracted, the yields of essential oil and Caryophyllene were 23.2 g/kg DM and 429.6 mg Caryophyllene/kg DM, respectively. The essential oil of DM showed an antioxidant effect even at a low concentration, and the concentration of the essential oil of SC₅₀, which means 50% of 2,2-diphenyl-1-picrylhydrazyl (DPPH) radical scavenging activity, was about 0.34%.

• The Korean Journal of Mycology
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• v.51 no.1
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• pp.51-56
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• 2023

Dendropanax trifidus belonging to the family Araliaceae, is a warm-temperate evergreen tree distributed in Jeju Island, Bogil Island, Geomun Island, Geoje Island, Wando, and Haenam in Korea. In June 2021, a root rot disease in which branches of Dendropanax trifidus seedlings turned brown and shrunk was discovered at the seedling cultivation facility in Naju-si, Republic of Korea. To identify the root rot fungus, three strains were isolated from the diseased tissues of seedlings and their mycological characteristics were investigated on potato dextrose agar. In addition, a molecular phylogenetic analysis was performed using sequences of the internal transcribed spacer (ITS) region and translation elongation factor 1-α (EF1-α) gene. The fungus was identified as Fusarium oxysporum. For pathogenicity test, the roots of seedlings were immersed in the conidia suspension of the strains and planted. After 20 days inoculation, root rot and browning symptoms were confirmed in the inoculated plants. This is the first report of F. oxysporum on D. trifidus in Korea.

• KSBB Journal
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• v.10 no.4
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• pp.393-400
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• 1995

As a part of the .study on the multiple propagation and use of Dendropanax mcrbifera, distribution of this tree and component analysis of Golden Lacquer were investigated to reappear the Golden Lacquer, traditional paints. These results were summarized as follows. The natural growth habitat of Dendropanax morbifera was observed in 8 sites(Wando, Sanghwangbong, etc.), we could be found that Duryun nountain in Haenam for the first time. Dendropunax morbifera has grown from 100 to 450m above the sea level, appeared high frequency in the 200m. The soil pH of natural growth site was pH 4.9 to pH 5.8. Dendropanax morbifera grew in the area containing considerable moisture in that its contents was 16.5% to 27.4%. In community observation, dominance and community index of Bogildo and Chejudo was 3.3 in the forest tree layer and Wando was showed in the sub-forest tree layer mainly. In the growth conditions, Bogildo appeared the highest growth state that diameter of height of human chest was 1.0 to 20cm and tree height was 0.2 to 9m. As result of IR and GC-MS analysis, main component of Golden Lacquer was ${\beta}$-selinene and capnellane-8-one.