• The Sea:JOURNAL OF THE KOREAN SOCIETY OF OCEANOGRAPHY
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• v.20 no.1
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• pp.53-62
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• 2015

To elucidate the microbial consortia responsible for the anaerobic methane oxidation in the methane hydrate bearing sediments, we compared the geochemical constituents of the sediment, the rate of sulfate reduction, and microbial biomass and diversity using an analysis of functional genes associated with the anaerobic methane oxidation and sulfate reduction between chimney site (UBGH2-3) on the continental slope and non-chimney site (UBGH2-10) on the basin of the Ulleung Basin. From the vertical profiles of geochemical constituents, sulfate and methane transition zone (SMTZ) was clearly defined between 0.5 and 1.5 mbsf (meters below seafloor) in the UBGH2-3, and between 6 and 7 mbsf at the UBGH2-10. At the UBGH2-3, the sulfate reduction rate (SRR) in the SMTZ exhibited was appeared to be $1.82nmol\;cm^{-3}d^{-1}$ at the depth of 1.15 mbsf. The SRR in the UBHG2-10 showed a highest value ($4.29nmol\;cm^{-3}d^{-1}$) at the SMTZ. The 16S rRNA gene copy numbers of total Prokaryotes, mcrA, (methyl coenzyme M reductase subunit A), and dsrA (dissimilatory sulfite reductase subunit A) showed the peaks in the SMTZ at both sites, but the maximum mcrA gene copy number of the UBGH2-10 appeared below the SMTZ (9.8 mbsf). ANME-1 was a predominant ANME (Anaerobic MEthanotroph) group in both SMTZs of the UBGH2-3 and -10. However, The sequences of ANME-2 were detected only at 2.2 mbsf of the UBGH2-3 where high methane flux was observed because of massive amount of gas hydrate at shallow depth. And Desulfosarcina-Desulfococcus (DSS) that is associated with ANME-2 was detected in 2.2 mbsf of the UBHG2-3. Overall results demonstrate that ANME-1 and ANME-2 are considered as significant archaeal groups related to methane cycle in the subsurface sediment of the East Sea, and ANME-2/DSS consortia might be more responsible for methane oxidation in the methane seeping region than in non-seeping region.

• Journal of Wetlands Research
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• v.9 no.1
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• pp.1-11
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• 2007

The objectives of this study are: (1) to compare the rates and pathways of organic matter minerlaization at stagnant freshwater wetland in Shiwha to highly irrigated coastal wetland in Ganghwa; and (2) to discuss the significance of irrigation into the sediment in controlling the organic carbon oxidation in Shiwha wetland. Concentrations of $CO_2$, $NH_4{^+}$ and $H_2S$ in the pore water of the Shiwha wetland were 3 times, 30 times, and 3 times higher than that in the pore water of the Ganghwa wetland, respectively. The ratio of Fe(III) to total reduced sulfur at the Ganghwa wetland was 12 times higher than at the Shiwha wetland. The results indicated that the Ganghwa wetland with frequent tidal inundation were relatively oxidized than highly stagnant Shiwha wetland. Rates of organic matter oxidation at the Ganghwa wetland ($0.039mM\;C\;h{-1}$) was 390 times higher than that at the Shiwha wetland ($0.0001mM\;C\;h{-1}$). Rates of sulfate reduction at the Shiwha wetland ($314{\sim}580nmol\;cm^{-3}\;d{-1}$) were comparable to the sulfate reduction at Ganghwa wetland ($2{\sim}769nmol\;cm^{-3}\; d{-1}$), whereas Fe(III) reduction rates were 1.7 times higher at the Ganghwa wetland ($0.1368{\mu}mol\;cm^{-3}\;d{-1}$) than at the Shiwha wetland ($0.087{\mu}mol\;cm^{-3}\;d{-1}$). The results implied that the water flow system of the Shiwha wetland was too stagnant to flush out the reduced pore water from the sediment, and thus anaerobic microbial respiration was limited by the availability of electron acceptors.

• Economic and Environmental Geology
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• v.38 no.3 s.172
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• pp.335-346
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• 2005

In this study, core sediments and pore water were analysed to identify the origin of organic matter and Bas in late Quaternary sediments from the northwestern Ulleung Basin of the East Sea. C/N and C/S ratios in the sediments show that the organic matter in the study area originated predominantly from marine algae. However, the results of Rock-Eval pyrolysis indicate that the organic matter has an origin of the land-plant (Type III), locating in the immature stage. These different results might be due to the heavy oxidizing of the organic matter during sinking down to the seafloor or after deposition in the sediments. Concentration of sulfate in the pore water decreases gradually with core depth, while concentration of $CH_4$ increases gradually with core depth. This indicates that sulfate reduction and methanogenesis occurred actively in the sediments. Also, it is likely that the compositions of $CH_4$ are characterized as a more biogenic origin, mostly caused by microbial activity, rather than a thermogenic one.

• Proceedings of the Korean Society of Soil and Groundwater Environment Conference
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• 2003.09a
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• pp.184-187
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• 2003

To solve the problems of operating passive mine drainage treatment systems, the In Adit Sulfate Reducing System(IASRS) was suggested. By placing the SAPS inside the adit, the condition of constant temperature of 10~15$^{\circ}C$ can be maintained. The experiments using the models made up by four sections showd good efficiencies in pH control and metal removal rate, but showed still low sulfate removal rate of about 30% with high COB in the begining of the operation.

• Microbiology and Biotechnology Letters
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• v.20 no.2
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• pp.233-235
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• 1992

The number of sulphate-reducing bacteria (SRB) in some of the tributaries to the Han River was determined by the most probable number method using Postgate's Medium E.Higher number of SRB were obtained in the streams to which industrial waste water is discharged than those receiving only domestic waste water.

• Journal of the Korean Society for Marine Environment & Energy
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• v.4 no.3
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• pp.16-27
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• 2001

We have studied the phosphorus geochemistry in sediments from anoxic environments of Lake Shihwa. The dominant sedimentary phosphorus forms were detrital apatite P and Al-bound p, whereas the amount of Fe-bound P was low because of anoxic condition. Based on the correlation between TP(Total Phosphorus) and OC(Organic Carbon), the behavior of sedimentary phosphorus was influenced by organic matters. It shows that dissolved and solid phosphorus concentrations, the flux of phosphate and the correlation coefficients between sedimentary phosphorus and organic matter In St. Cl were higher than those in St. C2. The results indicate that the concentrations and distributions of phosphorus In sediments were controlled by organic matters.

• Journal of the Mineralogical Society of Korea
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• v.24 no.2
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• pp.83-90
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• 2011

Removal of dissolved uranium by D. baculatum, a sulfate-reducing bacterium, and effects of trace metals such as manganese, copper, nickel, and cobalt were investigated. Total concentrations of dissolved uranium and trace metals were used by $50\;{\mu}M$ and $200\;{\mu}M$, respectively. Most dissolved uranium decreased up to a non-detectable level (< 10 ppb) MS during the experiments. Most of the heavy metals did nearly not affect the bioremoval rates and amounts of uranium, but copper restrained microbial activity. However, it is found that dissolved uranium rapidly decreased after 2 weeks, showing that the bacteria can overcome the copper toxicity and remove the uranium. It is observed that nickel and cobalt were readily coprecipitated with biogenic mackinawite.

• Economic and Environmental Geology
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• v.29 no.1
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• pp.65-75
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• 1996

Sulfate reduction and the precipitation of metal sulfides may have great potential to improve water quality of mine effluents in wetland treatment systems. Laboratory experiments using sulfate reducing bacteria (SRB) and limestone to treat effluents from the abandoned Dalsung tungsten-copper mine show that encouraging results, that have been attributed to sulfate reduction. Fe, Al, Cd, Cu and Zn are reduced to below detection limits with $99{\sim}100%$ metal removal rates, Mn is reduced by at least 90% to below 8.0 mg/l, and the pH is raised from 5.12 to 7.60 after 53 days of experiments. In the staged design, laboratory experiments are initiated to determine what would be reasonable substrate materials for remediation of the mine effluents. A substrate mixture containing 70% oak compost and 30% mushroom compost maintains $0.03{\sim}0.04mM$ of lactate, which provides good condition for the SRB granule. A downflow SRB wetland system is proposed as follows : 1) The lower part of the treatment system consists with a 25 cm thick layer of high quality (above 95% of $CaCO_3$) of limestone; 2) The geotextile (geonet) is recommended to be spread on the limestone bed to prevent clogging the limestones with the substrates; 3) The mixture of substrates with 70% oak and 30% spent mushroom composts, and SRB granules overlain on top of the geonet with 25 cm height. The sizes of the passive treatment systems are calculated according to metal loading and permeability criteria : 1) $220m^3$ ($15{\times}15{\times}1m$) for -1 level effluents; 2) $28m^3$ ($5.3{\times}5.3{\times}1m$) for -2 level; and 3) $2700m^3$ ($52{\times}52{\times}1m$) for the -3 level. The -3 level system needs to be broken down into 5 to 15 cells.

• Proceedings of the Korean Society of Fisheries Technology Conference
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• 2000.10a
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• pp.223-224
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• 2000

대하 양식장과 같이 물의 유동이 작고, 폐쇄성이 강한 지역, 더구나 유기물 혹은 질소, 인등의 영양염류 농도가 높은 부영양화 지역에서는 저질 중의 유기물 분해에 따른 산소소비는 저질환경을 산화상태로부터 환원상태로 이동시켜 황산염 환원에 의한 황화수소가 발생한다. 따라서 황화수소의 발생은 용존산소의 저하를 나타냄과 동시에 그 자체가 강한 독성을 가지고 있으며, 질소화합물 중의 암모니아 및 아질산과 같은 형태도 양식장의 생산억제 요인으로 작용한다(Gavis and Grant, 1986; Thompson et al., 1989). 그러나, 지금까지의 이들 오염물질, 특히 황화수소에 대한 연구는 미비하며, 대부분의 연구도 성체를 중심으로 이루어지고 있다. (중략)

• Journal of the Korea Organic Resources Recycling Association
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• v.7 no.2
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• pp.25-34
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• 1999

The effects of copper on the anaerobic degradation of propionate were studied using anaerobic batch reactors. The apparent inhibitory effects of copper on the anaerobic degradation of propionate could be observed from behaviors of intermediates, ultimate methane yield(UMY) and specific methanogenic activity(SMA) There was little inhibition at the concentration of $2.5mg\;Cu^{2+}/L$. Beyond this concentration, the inhibitory effects increased with increasing dose of coppers. The 50% inhibition of UMY and SMA occurred at copper dosage of 33.8 and $24.1mg\;Cu^{2+}/gVSS$, respectively. The inhibitory effect based on the UMY was gradually reduced with the operation time dueprobably to the acclimation of microorganisms and/or binding of the added copper by ligands(and possibly ion exchange sites)contained on the cell membrane and extracellular polymer matrix whereas it based on the SMA might exclude the this phenomena. Therefore, the methodology for interpretation of inhibition data based on the SMA was more accurated than the UMY. There was no inhibitory effect in batch reactors supplemented with sulfate due to an antagonistic action of the sulfate reducing bacteria. Propionate degradation was initially retarded for copper inhibited samples but it gradually degraded afterward. Based on the mass removal considering take into account the propionate to acetate conversion, propionate degradation may appeal more affected than acetate. This result revealed that the hydrogenotrophic methanogens were the most affected by copper.