• Proceedings of the Korean Institute of Navigation and Port Research Conference
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• 2023.11a
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• pp.241-241
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• 2023

• Korean Journal of Fisheries and Aquatic Sciences
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• v.28 no.2
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• pp.137-140
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• 1995

The pufferfish Takifugu obscurus (hwang-bok) was examined for toxicity. Forty-six specimens, which had Gaught at the Imjin River in 1992 and 1993, Korea, were collected and assayed for anatomical distribution of toxicity by the mouse assay method. Ovary and liver showed very strong toxicity, testis, intestine, gall bladder and spleen did moderate toxicity, muscles and skin did weak toxicity, and blood was non-toxic. The results of this study were different from those of Tani, who had examined the toxicity in 19 species of pufferfish, in terms of toxicity in testis, muscle, and skin. The toxicity of testis and muscle had been known to be non-toxic or weakly toxic previously, however, they were known to have weak or moderate toxicity. Therefore, careful attention should be taken to prevent food poisoning by pufferfish ingestion.

• Journal of fish pathology
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• v.17 no.2
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• pp.99-103
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• 2004

White spot disease by Cryptocaryon irritans broke out among cultured river puffer (Takifugu obscurus) cultured in sea water in Oct. 2001 and May 2004. Many white spots were easily observed on the gills with the naked eyes. The parasites attached on the gills at the base of the gill lamellae. The trophonts penetrated the interlamellar epithelium. Epithelial cell proliferation in interlamellar spaces occurred. PAS- positive mucous cells were prominent in the proliferated epithelium.

• Journal of Aquaculture
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• v.20 no.4
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• pp.278-288
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• 2007

Shrimp culture in Korea had been rapidly developed during 1990's and the production of farmed shrimp reached 3,268 mt from 2,605 ha in 2001. However the shrimp production decreased to 2,368 mt in 2004 because of the mass mortality due to outbreak of white spot syndrome virus (WSSV). WSSV which is one of the most serious threats associated with cultured shrimp around the world has given the economic damages to shrimp culture industry every year since it was found from the shrimp ponds in the west coast of the South Korea in 1993. Various polyculture technologies of shrimp with shellfish, finfish or seaweeds have been implemented to reduce economic damages by mass mortalities of shrimp. Among them, the polyculture of shrimp with carnivorous fish can suppress or delay the viral outbreak of shrimp ponds because the fish may selectively eat the moribund shrimps infected by virus. To determine the selective predatory effect of river puffer Takifugu obscures on WSSV infected shrimp, postlarvae of Litopenaeus vannamei and Fenneropenaeus chinensis. One-year old river puffers were stocked to four earthen ponds of $1,616-1,848\;m^2$ in surface area as followings: polyculture LvP, L. vannamei ($43.4/m^2$)+puffer ($0.22/m^2$); control Lv, L. vannamei ($46.9/m^2$); polyculture FcP, F. chinensis ($30.3/m^2$)+puffer ($0.25/m^2$); control Fc, F. chinensis ($24.6/m^2$). Ponds of control Fc and polyculture FcP had mass mortalities by WSSV outbreak on the $51^{st}$ and $57^{th}$ days of culture respectively. The shrimps of polyculture LvP and control Lv were harvested on the $95^{th}\;day$. Shrimp survival rates of polyculture LvP and control Lv were 32.4% and 18.2% respectively and shrimp productivity of polyculture LvP was 69.2% higher than that of control Lv. Concentration of nutrients (TAN, $NO_2-N$, $NO_3-N$) was maintained within optimal ranges for shrimp growth although that of polyculture ponds showed at least two times higher than that of control ponds. The results suggest that polyculture of L. vannamei with river puffer is higher than monoculture in survival rate and productivity. In addition, F. chinensis should be carefully cultured because this species shows much higher susceptibility to WSSV than L. vannamei.

• The Sea:JOURNAL OF THE KOREAN SOCIETY OF OCEANOGRAPHY
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• v.4 no.4
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• pp.298-304
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• 1999

Toxic effect of landfill leachate on the larvae and juvenile stage of the river puffer fish, Takifugu obscurus, were investigated in order to assess severity of environmental impact of leachate effluent on the general population of estuarine fishes. A short-term toxic experiment was designed to test both laboratory hatched larvae and juveniles (5, 10 and 15 mm in length) and in-situ juveniles (30 and 45 mm in length) using the leachate concentrations between 0 and 16%. Lethal concentrations of 50% mortality ($LC_{50}$) were observed using Spearman-karber Method. 24hr-$LC_{50}$ appeared at the leachate concentrations ranging from 3.03 to 8.57%, 48hr-$LC_{50}$ at 2.73 to 6.21 %, 72hr-$LC_{50}$o at 2.45 to 5.53%, and 96hr-$LC_{50}$ at 2.38 to 4.93%, respectively. Leachate concentrations between 0.69 and 1.51% induced 96hr-$LC_1$. Respiratory frequency was significantly affected even at low leachate concentrations between 0.5 and 1.0% (P < 0.05). These results suggest that the leachate effluents may cause a harmful impact on the physiology of river puffer fish, especially for younger cohorts.

• Journal of fish pathology
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• v.22 no.1
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• pp.67-73
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• 2009

It was examined whether the common belief that "cultured puffer fishes do not contain tetrodotoxin (TTX)", the major lethal substance that accidently causes death in consumers of those fishes, is true in river puffer fish Takifugu obscurus. In mouse bioassay, lethal levels of toxins were detected in the ranks: gonad>liver>intestine>muscle>skin in wild puffer fish. In contrast, no mortality occurred in the mouse bioassay on cultured fish. However, there were sleepiness, sluggish behavior, and hind limb paralysis with the tissue extracts of cultured fish suggesting the presence of TTX or other similarly acting toxins. An attempt to confirm the presence of TTX in cultured fish with high performance liquid chromatography (HPLC) was not very successful. The results suggest possible existence of TTX toxins or similarly acting toxins.

• Korean Journal of Ichthyology
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• v.19 no.3
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• pp.185-192
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• 2007

The present experiment was carried out to study the effects of light intensity on growth, survival, caudal fin shape, body composition, skin color and blood component of river puffer, Takifugu obscurus. Four light intensities (0, 500, 1,000, 2,000 lx) were tested in duplicates for 60 days. Specific growth rate (SGR), daily feeding rate (FR) and food coefficient (FC) were reduced following to the decrease of light intensity. However in survival rate there was not significantly different among groups. The damage extent of caudal fin was increased in more strong light intensity. In body composition, moisture and crude protein were only significantly difference according to light intensity. In body color, L and a were the highest in 0 lx. In blood analysis, the content of AST, ALT, GLU significantly increased in more powerful the intensity groups. It is concluded that the supplement of low light intensity (e.g. 0 lx) recommended for the commercial scale aquaculture of river puffer in land-based tank without growth and survival retardation.

• Journal of fish pathology
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• v.11 no.2
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• pp.119-127
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• 1998

Two species of the parasitc copepods, Clavellopsis hugu (Lemaeopodidae; Siphonostomatoida) and Taeniacanthus yamagutii (Taeniacanthidae; Poecilostomatoida) from cultured Korean fish, Takifugu obscurus, is described and reported for the first time in Korea. The parasite was recovered from the buccal cavity, nose and skin. Clavellopsis hugu is easily identified by the body shape, 2nd antenna, mandible and 2nd maxillae. In female, cephalothorax flexed dorsally, second antenna biramose, mandible with serrate blade, second maxillae completely fused at base of cephalothorax. In male, cephalothorax and trunk fused into an ovoid, two pairs of maxillipeds chelate. The most distinctive features of Taeniacanthus yamagutii are terminal process of second maxilla stout, maxilliped claw with conspicuous digitiform process at base and a setiform element at the tip of each exopod spine of legs 2-4. Both parasitic copepod species are new to the Korean fauna. The korean fish, Takifugu obscurus should be considered as an additional host of this species because these parasite were recovered from the western coast of Korea (Boryung Hatchery).

• Development and Reproduction
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• v.15 no.2
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• pp.77-85
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• 2011

To investigate the influence of density and feeding frequency (food amount) on growth, cannibal dynamics and survival of puffer Takifugu obscurus (TL $25.0{\pm}0.5$ mm, BW $0.53{\pm}0.03$ g) during early life history, we conducted one experiment composed with 6 density groups set up as 1.43 inds./${\ell}$, 2.14 inds./${\ell}$, 2.86 inds./${\ell}$, 3.57 inds./${\ell}$, 4.29 inds./${\ell}$ and 7.14 inds./${\ell}$. The experiment was triplicately performed in 1 ton FRP tank for 30 days. Daily food intake (DFI), feed efficiency (FE), growth, cannibal frequency, and survival rates were examined. Although not find any significant relation of density to DFI and FE, we found that growth decreased significantly with the increasing of density under 3.57 inds./${\ell}$, but significantly increased with the increasing of density over 4.29 inds./${\ell}$. Cannibal frequency and daily mortality rate was significantly increased with increasing density and decreasing feeding frequency, inducing the most poor survival rate in the highest density group and low feeding frequency. Therefore, we concluded that the density and the feeding frequencies affect sibling cannibal dynamics and survival of puffer stock.

• Korean Journal of Ichthyology
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• v.30 no.3
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• pp.144-154
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• 2018

This study investigated the characteristics of fish communities at the Hangang River in Seoul from Jun 2016 to March 2017. During the survey period, there were 56 species of 17 families collected from 5 survey stations. The species that appeared mainly in the brackish water was Coilia nasus, Leiocassis nitidus, Lateolabrax maculata, Repomucenus olidus, Trachidermus fasciatus, Mugil cephalus, Chelon haematocheilus, Synechogobius hastus, Tridentiger bifasciatus and Takifugu obscurus. The migration fish of Anguilla japonica, C. nasus, Plecoglossus altivelis, Hypomesus nipponensis and T. obscurus appeared in this surveyed area. Korean endemic species were 11 species (17.9%) and accounted for a low rate. There were 6 species (10.7%) exotic species including Cyprinus carpio, Carassius cuvieri, Hypophthalmichthys molitrix, Megalobrama amblycephala, Lepomis macrochirus and Micropterus salmoides. The dominant species by stations were Acheilognathus gracilis (St. 1) and Hemibarbus labeo (St. 2, 3, 4, 5). Regarding the tolerance guild according to water quality, there were 3 (7.1%) sensitive species, 24 (42.9%) intermediate species, and 28 (50.0%) tolerance species. Twenty (35.7%) were insectivore species, 18 (32.1%) were omnivore species, 14 (25.0%) were carnivore species, and 4 (7.1%) were herbivore species, indicating the dominating presence of insectivore and omnivore fish species.