• 약학회지
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• 제57권4호
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• pp.293-298
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• 2013

Hwangryunhaedok-tang (HRT) is a traditional herbal medicine, which has been known as a useful prescription for anti-biotic, anti-inflammatory, anti-oxidative and immunosuppressive activity. In this study, the variation in the amount of eight bioactive components of Hwangryunhaedok-tang (HRT) and its fermentation HRT with Lactobacillus casei KFRI 127, Lactobacillus curvatus KFRI 166 and Lactobacillus confuses KFRI 227 was investigated via high-performance liquid chromatography coupled with diode array detection (HPLC-DAD). Simultaneous qualitative and quantitative analysis of eight bioactive components; geniposide, genipin, baicalin, wogonoside, palmatine, berberine, baicalein and wogonin was achieved by comparing their retention times ($t_R$) and UV spectra with those of the standard components. All calibration curve of standard components showed good linearity ($r^2$ >0.979). As a result, the geniposide amount was $15.52{\pm}0.19{\mu}/mg$ that as a main components in HRT. The wogonoside was decreased by 29.28~58.35% with Lactobacillus casei KFRI 127 and L. confuses KFRI 227 ($3.17{\pm}0.31{\mu}g/mg$ and $3.55{\pm}0.13{\mu}g/mg$) compared with the original HRT ($5.02{\pm}0.14{\mu}g/mg$). Otherwise wogonin was increased by 16.28~41.86% with Lactobacillus casei KFRI 127 and L. confuses KFRI 227 ($0.61{\pm}0.01{\mu}g/mg$ and $0.50{\pm}0.02{\mu}g/mg$) compared with the original HRT($0.43{\pm}0.00{\mu}g/mg$). HRT fermented with L. casei KFRI 127 and L. confuses KFRI 227 were evaluated as creating the changes in wogonoside to that aglycon wogonine. In the fermented HRT using Lactobacillus acidophilus KFRI 166, the genipin was only detected, among 3 species of fermentation strains. Thus, these results considered that the strains 166 were exhibited the remarkable changes in genipin.

• 대한한방내과학회지
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• 제24권2호
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• pp.220-232
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• 2003

Objective : The purpose of this study was to analyze the effects of HwangRyunHaeDok-Tang and combinations of constituent herbs on the arterial contraction. Methods : In order to investigate the effects Scutellariae Radix. Coptidis Rhizoma, Phellodendri Cortex and Gardeniae Fructus, in which one of them, two of them, and all of them, were used to exam. Results : The results were summarized as follows; 1. HwangRyunHaeDok-Tang significantly inhibited the contraction of artery induced by Norepinephrine(NE). However the atonic effect was slightly blunted when the vascular endothelial cell was removed. No significant change in the atonic effect of HwangRyunHaeDok-Tang was found when $_L-NNA$ was used as a preliminary treatment. These results indicate that the vascular atonic effect by HwangRyunHaeDok-Tang is slightly dependent on the endothelial cell, and that the HwangRyunHaeDok-Tang works directly to the vascular smooth muscle in creating the vascular atonic effect. 2. The pretreatment of HwangRyunHaeDok-Tang extract significantly inhibited the contractile response to additive application of $Ca^{2+}$ in the strips which were contracted by NE in $Ca^{2+}$-free solution. 3. HwangRyunHaeDok-Tang extract increased the contraction of arterial smooth muscle induced by KCl. Therefore, it can be concluded that HwangRyunHaeDok-Tang may block the NE-receptor or receptor-operated $Ca^{2+}$ channel. 4. It was determined that Scutellariae Radix, Coptidis Rhizoma and Phellodendri Cortex among the ingredients of HwangRyunHaeDok-Tang have a vascular atonic effect. In addition, those ingredients plays a role in strengthening the atonic effect by working with other herbal medicines. Gardeniae Fructus causes the blood vessel to contract. but it does not influence the atonic effects of other herbal medicines. However Gardeniae Fructus tends to inhibit the vascular atonic effect of Phellodendri Cortex. Conclusion : Based on the above results, it can be said that HwangRyunHaeDok-Tang can be applied to cure hypertension considering those three herbs have significant effects of relaxation.

• 생명과학회지
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• 제17권3호통권83호
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• pp.334-339
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• 2007

본 연구는 우황청심원을 비롯한 상용되는 20종의 한약제제를 대상으로 9종의 시토크롬 동종효소에 대한 대사능의 저해정도를 고속 스크리닝 기법을 이용하여 탐색함으로써, 한약제제와 약물의 병용으로 인한 약물 상호작용 가능성을 평가하고자 하였다. 인체 간 마이크로좀 시료에 9종의 주요 시토크롬 약물대사효소의 지표약물과NADPH-generating system및 한약제제(500 ${\mu}g/ml$)를 첨가한 후 $37^{\circ}C$에서 15분간 반응시켜 생성된 각각의 대사물을 LC/MS/MS를 이용하여 정량하여 시토크롬 동종효소 활성의 변화를 평가하였다. 그 결과 우황청심원 현탁액 및 황련해독탕 물 추출물이 각각 CYP2B6 및 CYP2D6 효소 활성을 선택적으로 강력하게 저해하였다. 이러한 결과는 약국에서 쉽게 구입할 수 있는 한약제제들 중 일부는 인체 간 시토크롬 활성 저해능을 가지고 있고, 이들 효소에 의해 대사되는 약물과의 병용 복용시 약물상호작용 발생 가능성이 있음을 의미한다. 향후 한약제제에서 저해능을 나타내는 주된 성분을 규명하여 이 성분의 저해능과 저해 기전을 살피는 노력이 필요할 것이다.

• 대한한방부인과학회지
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• 제29권1호
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• pp.1-13
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• 2016

Objectives The purpose of this study is to find out the effect of various concentrations of Hwangnyeonhaedok-tang (HTT) pharmacopuncture on ST25 (天樞) in rats with dextran sulfate sodium (DSS)-induced colitis.Methods Colitis was experimentally induced by feeding rats with water mixed with 5% (w/v) DSS for 20 days. The rats were divided into 5 groups: the normal group (Nor, n=5), the control group - colitis induced rats with no treatment (Con, n=5), the acupuncture group - colitis induced rats with acupuncture applied on ST25 (Acu, n=5), the pharmacopuncture group 1 - colitis induced rats with 0.729 mg/250 g/40 μl of pharmacopuncture applied on ST25 (PA-1, n=5), the pharmacopuncture group 2 - colitis induced rats with 3.645 mg/250 g/40 μl of pharmacopuncture applied on ST25 (PA-2, n=5). The changes in weight, excrement concentration and hemafecia were observed 5 times every 2 days. The colon lengths were measured from appendix to the end of colon after the experiment. Hematological and serological exams were conducted the day after the last treatment by cardiac puncturing anesthetized rats.Results ST25 is the abdominal front point (募穴) of large intestine meridian and is known to have effect in colitis. Various concentrations of HTT pharmacopuncture (HTTP) applied on ST25, in rats with DSS-induced colitis inhibited decrease in colon lengths and body weight in both PA-1 and PA-2 groups. Hematological and serological exam results also showed that HTTP has significant effect on colitis in both PA-1 and PA-2 groups.Conclusions Colon lengths were significantly increased in the acupuncture group, PA-1 group and PA-2 group, compared to the control group. The body weight was significantly increased (p<0.05) in PA-2 group after the first treatment, compared to the control group. TNF-α, IL-6, AST were significantly decreased in PA-1 and PA-2 groups, compared to the control group.

• 대한한방부인과학회지
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• 제27권3호
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• pp.1-11
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• 2014

Objectives: Hwangryunhaedok-tang (HH) is a representative heat clearing multiherb prescription. We evaluated the protective effect of HH against heat stress exposure in mice. Methods: Six weeks old ICR mice were used for this study. After $43^{\circ}C$ heat stress for 15 minutes, we evaluated the changes of motor activity and c-fos expression level to determine the proper heat stress and evaluation time. The subjects were divided into 4 groups (1. control group, 2. heat stress group with normal saline, 3. heat stress group with administration of 100 mg/kg, and 4. heat stress group with administration of 1000 mg/kg). After oral administration of HH once a day for 3 days, 2, 3, 4 groups were exposed to $43^{\circ}C$ heat stress for 15 minutes. Then, we evaluated the motor activity for 120 minutes and analyzed the c-fos expression using western blot. We investigated the effect of HH and its ingredients on c-fos expressions after heat stress. The mice were administrated HH, Scutellariae Radix, Coptidis Rhizoma, Phellodendri Cortex, and Gardeniae Fructus for 3 days. After one hour of last treatment, the mice were exposed to heat stress at $43^{\circ}C$ for 15 min. After two hours, the hypothalamus was dissociated and lysed to measure c-fos expression. Results: After oral administration of HH for 3 days, motor activity was recovered insignificantly. 100 mg/kg HH treatment reduced the c-fos expression after heat stress but insignificantly. Among the ingredients of HH, Coptidis Rhizoma and Scutellariae Radix treatment groups significantly reduced the c-fos after stress. Conclusions: These results show that Hwangryunhaedok-tang may be effective to reduce the heat stress response.

• 대한중풍순환신경학회지
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• 제20권1호
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• pp.65-74
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• 2019

■ Objectives The purpose of this case study is to report the effectiveness of Korean medicine and pharmacopuncture in the treatment of pressure ulcer. ■ Methods The patient with pressure ulcer was treated with herbal medicine, Hwangryunhaedoktang pharmacopuncture, acu-moxi treatment. We started herbal medicine mainly Taklisodok-um for pressure ulcer, but changed to Paljung-san due to urinary tract infection on Day 67. During the daily dressing, we inject pharmacopuncture solution subcutaneous ulcer area and spray solution on the cavity of right pressure ulcer. The severity of pressure ulcer was assessed using[NPUAP] pressure ulcer stage, healing rate of pressure ulcer and depth of right side ulcer cavity on Day1, Day15, Day40, Day73, Day95. ■ Results After treatment, the total size of the pressure ulcer was reduced from 90(10*9) to 56(8*7)cm², healing rate of pressure ulcer increased to 37.8%, depth of pressure ulcer was reduced from 3.5 to 2cm. Though NPUAP stage(Gr 4) was not changed, size and color of pressure ulcer definitely improved compared to Day1. ■ Conclusion This study suggested taht Korean medical treatment could be effective option for treating grade 4 pressure ulcer.

• 대한본초학회지
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• 제33권2호
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• pp.9-18
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• 2018

Objectives : Hwangryunhaedok-tang (HRHDT) is one of the well-known prescription herbal drugs of Korean herbal medicine, which has been widely used for the treatment of various bacterial and inflammatory diseases. This study was conducted in order to develop the tablet formulations of HRHDT and compare its efficacy with the other commercial formulations. Methods : Corresponding herbal medicines comprising of HRHDT were extracted with water for 3 hr at $95{\sim}100^{\circ}C$ and then vacuum dried. Subsequently, some pharmaceutical excipients such as microcrystalline cellulose, croscarmellose sodium, magnesium stearate, etc were used to prepare the HRHDT tablets. The contents with characterizing components of HRHDT tablet was compared with the HRHDT decoction. The contents of characterizing components were analyzed with HPLC. Furthermore, we investigated the anti-inflammatory and anti-oxidative abilities of two different commercial HRHDT granules (HJP-1 and HJP-2) and were compared with that of the formulated HRHDT tablets. The anti-oxidant properties of HRHDR were studied using the 1,1-diphenyl-2-picryhydrazyl (DPPH) radical, contents of total flavonoid and polyphenol. In addition, based on this result the anti-inflammatory effects have verified by mechanism from LPS- treated Raw264.7 macrophages. Results : The results demonstrated that HRHDT tablets showed more anti-inflammatory and anti-oxidative effects than HJP-1, HJP-2. Moreover, it showed more superior effects in terms of dose, usability and stability than the granules. Conclusion : Hence, we concluded that in order to improve the quality and efficacy of the Korean herbal medicine, it is necessary to develop appropriate methods and establish standardized techniques for the development of good formulations.

• 대한한방부인과학회지
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• 제28권2호
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• pp.193-203
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• 2015

목 적: 상열감과 발한과다는 갱년기 증후군의 대표적 증상이다. 본 연구에서는 약침을 이용한 수화조절법을 사용하여 갱년기 증후군 환자의 상열감과 발한과다 증상 감소에 유의한 효과를 얻어 이를 보고하고자 한다. 방 법: 황련해독탕 약침을 양측 견정(GB21), 풍지(GB20) 혈에 각각 0.1 cc씩 총 0.4 cc를 주입하고, BUM(웅담, 우황, 사향)약침은 전중(CV17), 중완(CV12), 기해(CV16)혈에 각각 0.05cc씩 총 0.15cc를 주입한다. 시술은 매일 시행하였으며, 증상에 관하여 상열감점수(Hot flush score), 발한에 대한 Visual Analogue Scale(VAS) 및 발한 부위를 기록하여 증상변화 정도를 평가하였다. 결 과: 1번 증례는 약침 수화조절법 시행 후 상열감점수는 24에서 4로, 발한에 관한 VAS는 7에서 2로 감소하였다. 상열감 및 발한과다 증상의 감소와 더불어 손발이 화끈거리는 증상도 함께 감소되었다.2번 증례는 약침 수화조절법 시행 후 상열감점수가 28에서 2로, 발한에 관한 VAS는 10에서 3으로 감소하였다. 상열감 및 발한과다 증상의 감소와 더불어 불안, 우울, 분노 증의 정서적 증상도 70%정도 감소하였다.3번 증례는 약침 수화조절법 시행 후 상열감점수가 8에서 1로, 발한에 관한 VAS는 6에서 1로 감소하였다. 상열감 및 발한과다 증상의 감소와 더불어 두통, 가슴 답답함 등의 증상도 함께 소실되었다.모든 증례에서 발한 부위의 변화는 없었다. 결 론: 본 증례에서 약침을 이용한 수화조절법은 갱년기 상열감과 발한과다 증상을 감소시키는 효과가 있었으며 부가적으로 호소하던 증상도 감소하거나 소실되는 효과가 있었다. 그러므로 약침을 이용한 수화조절법은 상열감과 발한과다를 호소하는 갱년기 증후군 환자에게 효과적인 치료법이라 사료된다.