대한본초학회지 (The Korea Journal of Herbology)

  • 제33권2호
  • /
  • Pages.9-18
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  • 2018
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  • 1229-1765(pISSN)
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  • 2288-7199(eISSN)
대한본초학회 (The Korea Association of Herbology)
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황련해독탕을 함유하는 정제 개발과 성분함량 및 약리효과 평가

Evaluation on Pharmacological Effects and Compound Contents of Hwangryunhaedok-tang formulation for Tablet

  • 이지범 (한약진흥재단, 한약제제팀) ;
  • 최혜민 (한약진흥재단, 한약제제팀) ;
  • 김종범 (한약진흥재단, 한약제제팀) ;
  • 김정옥 (한약진흥재단, 한약제제팀) ;
  • 문성옥 (한약진흥재단, 한약제제팀) ;
  • 이화동 (한약진흥재단, 한약제제팀)
  • Lee, Ji-Beom (Korean Medicine Preparation Team National Development Institute of Korean Medicine (NIKOM)) ;
  • Choi, Hye-Min (Korean Medicine Preparation Team National Development Institute of Korean Medicine (NIKOM)) ;
  • Kim, Jong-Bum (Korean Medicine Preparation Team National Development Institute of Korean Medicine (NIKOM)) ;
  • Kim, Jung-Ok (Korean Medicine Preparation Team National Development Institute of Korean Medicine (NIKOM)) ;
  • Moon, Sung-Ok (Korean Medicine Preparation Team National Development Institute of Korean Medicine (NIKOM)) ;
  • Lee, Hwa-Dong (Korean Medicine Preparation Team National Development Institute of Korean Medicine (NIKOM))
  • 투고 : 2017.12.08
  • 심사 : 2018.03.15
  • 발행 : 2018.03.30
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초록

Objectives : Hwangryunhaedok-tang (HRHDT) is one of the well-known prescription herbal drugs of Korean herbal medicine, which has been widely used for the treatment of various bacterial and inflammatory diseases. This study was conducted in order to develop the tablet formulations of HRHDT and compare its efficacy with the other commercial formulations. Methods : Corresponding herbal medicines comprising of HRHDT were extracted with water for 3 hr at $95{\sim}100^{\circ}C$ and then vacuum dried. Subsequently, some pharmaceutical excipients such as microcrystalline cellulose, croscarmellose sodium, magnesium stearate, etc were used to prepare the HRHDT tablets. The contents with characterizing components of HRHDT tablet was compared with the HRHDT decoction. The contents of characterizing components were analyzed with HPLC. Furthermore, we investigated the anti-inflammatory and anti-oxidative abilities of two different commercial HRHDT granules (HJP-1 and HJP-2) and were compared with that of the formulated HRHDT tablets. The anti-oxidant properties of HRHDR were studied using the 1,1-diphenyl-2-picryhydrazyl (DPPH) radical, contents of total flavonoid and polyphenol. In addition, based on this result the anti-inflammatory effects have verified by mechanism from LPS- treated Raw264.7 macrophages. Results : The results demonstrated that HRHDT tablets showed more anti-inflammatory and anti-oxidative effects than HJP-1, HJP-2. Moreover, it showed more superior effects in terms of dose, usability and stability than the granules. Conclusion : Hence, we concluded that in order to improve the quality and efficacy of the Korean herbal medicine, it is necessary to develop appropriate methods and establish standardized techniques for the development of good formulations.

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