• Journal of Plant Biotechnology
• /
• v.40 no.1
• /
• pp.18-26
• /
• 2013

Genetically modified (GM) rice Agb0101, which expresses the insecticidal toxin modified cry1Ac (mcry1Ac1) gene, was developed by the Rural Development Administration in Korea. To monitor the probable release of Agb0101 in the future, it is necessary to develop a reliable detection method. Here, we developed the PCR detection method for monitoring and tracing of GM rice. The primer pair (RBEgh-1/-2) from a starch branching enzyme (RBE4) gene was designed as an endogenous reference, giving rise to an expected PCR amplicon of 101 bp. For the qualitative PCR detection, construct- and event-specific primers were designed on the basis of integration sequence of T-DNA. Event-specific PCRs amplified specifically 5'- or 3'-junction region spanning the native genome DNA and the integrated gene construct, while none of amplified product was shown on crops, rice varieties, and other insect-resistant transgenic rice lines. The event-specific real-time PCR method was performed using TaqMan probe and plasmid pRBECrR containing both rice endogenous gene RBE4 sequence and 5'-junction sequence as the reference molecule. The absolute limit of quantification (LOQ) of real-time PCR was established with around 10 copies for one plasmid molecule pRBECrR. Thereafter, the different amounts of transgenic rice (1, 3, 5, and 10%, respectively) were quantified by using the established real-time PCR method, with a range below 19.55% of the accuracy expressed as bias, 0.06-0.40 of standard deviation (SD) and 3.80-7.01% of relative standard deviations (RSD), respectively. These results indicate that the qualitative and quantitative PCR methods could be used effectively to detect the event Agb0101 in monitoring and traceability.

• Pediatric Infection and Vaccine
• /
• v.5 no.2
• /
• pp.239-244
• /
• 1998

Purpose : The incidence of hepatitis A virus infection in Korea has decreased recently. However, the prevalence in children and young adults is on an increasing trend in Inchon-Kyunggi prefecture. Economic development that leads to better living conditions and improves hygiene facilities has reduced the rate of HAV infection. This study was designed to evaluate the recent seroprevalence rate of hepatitis A in children and adolescents. Methods : Study population consisted of 612 children and adolescents aged 1 to 17 years in Inchon-Kyunggi prefecture. Serum samples were collected between 1996 and 1997 and tested for anti HAV by Microparticle Enzyme Immunoassay(MEIA). Results : According to age, the seropositive rates were 10.5%(male 13%, female 6.7%) in 1 year-old group, 0% in 3, 5, 11 and 13 year-old group, 6%(male 8%, female 4%) in 15 year-old group, and 11%(male 14%, female 8%) in 17 year-old group. Conclusion : This study showed that seropositive rate of HAV infection increased with age. While it revealed extremely low incidence of HAV infection in children less than 13 years old, they are also likely 10 be exposed 10 the infection. Our findings suggest that the effective administration of vaccination is needed in selected and high risk groups.

• Korean Security Journal
• /
• no.14
• /
• pp.517-534
• /
• 2007

Since the beginning of human society, there have always been struggles and competitions for survival and prosperity, terrorism is not a recent phenomenon, however in modern times it has progressed to reflect the advances in civilization and power structures. At the time of the 9.11 terrorist attacks in the U.S. A., a new world order was in the process of being established after the breakdown of the Cold War era. The attacks drove both the Western and the Islamic worlds into heightened fear of terrorism and war, which threatened the quality of life of the whole mankind. Through two war campaigns against the Islamic world, it seems the U.S. has been pushing its own militaristic security road map of the Greater Middle East democratic initiative, justifying it as a means to retaliate and eradicate the terrorist threats towards themselves. However, with its five-year lopsided victories that cost the nation almost four thousand military casualties, and the war expenses that could match the Vietnam war, the U.S. does not yet seem to be totally emancipated from the fears of terrorism. Terrorism, in itself, is a means of resisting forced rules a form of alternative competition by the weak against the strong, and a way of expressing a dismissive response against dictatorial ideas or orders which allow for no normal changes. Intrinsically, the nature of terrorism is a reaction opposing power logics. Confronted with the absolute military power of the U.S., the Islamic strategies of terrorism have begun to rapidly evolve into a new stage. The new strategies take advantage of their civilization and circumstances, they train and inspire their front-line fighters on the Internet, and issue their orders through the clandestine network of the Al Qaeda operatives. These spontaneously generated strategies have been gained speed among the second, and third Islamic generations, many of whom are now spread throughout western societies. This represents a failure of the power-driven, one-sided overseas security initiatives by the U.S., and is creating a culture of fear and distrust in western societies. It is feared that the U.S. war campaigns have made the clash of religions far worse than before, and may ever lead to global ethnic separations and large-scale population movements. Eventually, it may result in the terrorist groups, enlarged and secretly supported by the huge sums of oil money, driving all mankind into a series of irreparable catastrophes.

• Journal of The Korean Association For Science Education
• /
• v.35 no.4
• /
• pp.629-648
• /
• 2015

This study is a case study examining how research-based 'authentic' science education program contextually facilitates students' learning on NOS as a process. We developed 'Becoming a Scientist' mentor-mentee program and applied it to six Korean 7th graders for 8 months. A mentor, who is also a researcher, provided scaffolding and coaching, and her mentees were to perform the whole process of science research, including selecting the research subject and questions, planning research design, doing experiments, collecting and analysing data, writing research paper, and experiencing poster presentation at an academic conference. The research questions are 1) What would the students experience at every step of their research process?, and 2) Which perceptions would they construct NOS as a process? Data include classroom observations, interview, mentor's journal, and students' learning products. The results show that the mentees have experienced their views of NOS as a process in various ways such as role of research question and purpose, validity of measured value, researcher's subjectivity in interpreting data, experience of making public and peer review, and significance of academic conference. This study has shown that students' actual experience in scientific research enhanced their views about NOS as process without explicit and reflective approaches. We defined 'authenticity' associated with not only with its similarity to what scientists do but to learner's identity as scientific researcher. Based on the situated learning theory, this study sheds light on the necessity of reconsideration about the meaning of authenticity and embodying authentic context in science education for better NOS learning.

• Journal of the Korean Society of Food Science and Nutrition
• /
• v.46 no.9
• /
• pp.1071-1080
• /
• 2017

This study examined the adhesiveness of beneficial intestinal bacteria to whole-grains using confocal scanning laser microscopy (CLSM), to demonstrate the prebiotic effects of whole-grains, and to develop prebiotic puffed snacks with these whole-grains. CLSM has been used to observe the adhesiveness of Lactobacillus acidophilus, which belongs to beneficial intestinal bacteria, to whole-grain powders using optical sectioning techniques. The enhanced effects on the growth of beneficial intestinal bacteria with the hot water grain extract were verified using an indirect count method. Finally, a puffed snack was produced with the prebiotic effect and the quality was evaluated by checking the chromaticity and degree of hardness. As a result, L. acidophilus exhibited adhesive ability to whole-grain powders and growth of selected beneficial intestinal bacteria were improved significantly. The Hunter L value of the developed puffed snack increased when seasoning was added. The hardness of the puffed snack with seasoning was higher than that of the control. The results of a sensory evaluation showed that the puffed snack with seasoning was highly rated in the overall preference compared to the control.

• Asia-Pacific Journal of Business Venturing and Entrepreneurship
• /
• v.12 no.6
• /
• pp.111-122
• /
• 2017

This study analyzes the current state of entrepreneurship education and start-up foundations by country in order to find ways to improve the domestic entrepreneurial environment and to promote the recognition of desirable entrepreneurship practices. It also investigates the relationship between entrepreneurship, entrepreneurial will, and the level of opportunity-based entrepreneurships, by using data from the 2016 Global Entrepreneurship Trend Report (GETR). First, the results show the urgent need for the expansion of entrepreneurship education in Korea. In the GETR category of 'experience of entrepreneurship education in elementary, middle and high schools', Korea was ranked very low (19th place), among the 20 countries. In the 'college' and 'lifelong entrepreneurship education' categories, it procured a mid-level ranking (15th). While entrepreneurship education for all ages is being promoted globally, entrepreneurship education for middle-aged individuals in Korea is relatively weak. This implies that the expansion of entrepreneurship education to lifelong education and education for employees and retirees is required. Second, the individual's entrepreneurial intention in Korea was 3.8 points, implying a mid-level ranking (15th), and it ranked the lowest in terms of opportunity-based entrepreneurship (20th). In comparison to China (4.55) and the United States (4.01), the entrepreneurial intention of Koreans was found to be low. The level of opportunity-driven entrepreneurship was also found to be very low, compared to China (4.35), Japan (4.04) and the United States (4.59). In general, the proportion of the level in opportunity-driven entrepreneurship, increases from the factor-driven and efficiency-driven, to the innovation-driven type. In Korea, the percentage of entrepreneurial ventures centered around involuntary entrepreneurship and small businesses is high. It is also interpreted that opportunity-based entrepreneurships are low in number because of this high proportion of involuntary start-up and small businesses. Last, the entrepreneurial intention in all types (factor-driven, efficiency-driven, and innovation-driven) was exceptionally high. It has been confirmed that exposure to all entrepreneurship education (elementary, junior high, university, and lifelong education) in innovation-driven countries, greatly increases entrepreneurial intention. In the case of Korea, which is an innovation-driven country, qualitative improvement based on quantitative expansion of entrepreneurship education is expected to be a major driving force for individuals' entrepreneurial intention to obtain a mid-level ranking (15th).

• Journal of Korean Society of Forest Science
• /
• v.83 no.3
• /
• pp.410-423
• /
• 1994

Seasonal and diurnal changes of stomatal diffusive resistance(S.D.R.) and transpiration rate(T.R.) were investigated for determining the ecophysiological water relations of economic tree species subjected to chronic water stress or exogenous abscisic acid treatment. Four species of oaks including Quercus serrata, Q. mongolica. Q. acutissima, and Q. variabilis were used as the experimental materials and also Fraxinus rhynchophylla was studied together with oaks. Stomatal diffusive resistances were repeatedly measured on the containerized 1-0 year seedlings subjected to two kinds of soil moisture regime (wet and dry) in June, August, and September by LI-1600 Steady State Porometer of LI-Cor, Inc.. Exogenous abscisic acid (ABA) solutions of 0.5 mM and 0.05 mM in July and August, respectively, were absorbed into shoots cut from the containerized seedlings for determining their effects on stomatal behavior and transpiration. Most of measurements in stomatal diffusive resistance maintained about 5 s/cm in the morning after sunrise despite of different treatments. But the values fluctuated frequently to high level above 20 s/cm through the afternoon until sunset in the seedlings subjected to dry soil moisture regime. Despite of various treatments and environmental conditions, stomatal diffusive resistances of Q. variabilis were more stable than those of Q. serrata or Q. acutissima. Their values of F. rhynchophylla changed more irregularly in comparison with those of oak species. Exogenous abscisic acid absorbed into shoots cut from seedlings increased stomatal diffusive resistance obviously in most of the species studied. The stomatal responses to abscisic acid treatment were more sensitive in July especially in Q. serrata than in Q. variabilis and Q. acutissima. But the effects of ABA treatment were presented more remarkably in Q. acutissima in August. The responses to abscisic acid were not certain in F. rhynchophylla because of their various fluctuation patterns.

• Journal of Korea Water Resources Association
• /
• v.53 no.10
• /
• pp.845-859
• /
• 2020

Climate change and recent heat waves have drawn public attention toward other environmental issues, such as water pollution in the form of algal blooms, chemical leaks, and oil spills. Water pollution by the leakage of chemicals may severely affect human health as well as contaminate the air, water, and soil and cause discoloration or death of crops that come in contact with these chemicals. Chemicals that may spill into water streams are often colorless and water-soluble, which makes it difficult to determine whether the water is polluted using the naked eye. When a chemical spill occurs, it is usually detected through a simple contact detection device by installing sensors at locations where leakage is likely to occur. The drawback with the approach using contact detection sensors is that it relies heavily on the skill of field workers. Moreover, these sensors are installed at a limited number of locations, so spill detection is not possible in areas where they are not installed. Recently hyperspectral images have been used to identify land cover and vegetation and to determine water quality by analyzing the inherent spectral characteristics of these materials. While hyperspectral sensors can potentially be used to detect chemical substances, there is currently a lack of research on the detection of chemicals in water streams using hyperspectral sensors. Therefore, this study utilized remote sensing techniques and the latest sensor technology to overcome the limitations of contact detection technology in detecting the leakage of hazardous chemical into aquatic systems. In this study, we aimed to determine whether 18 types of hazardous chemicals could be individually classified using hyperspectral image. To this end, we obtained hyperspectral images of each chemical to establish a spectral library. We expect that future studies will expand the spectral library database for hazardous chemicals and that verification of its application in water streams will be conducted so that it can be applied to real-time monitoring to facilitate rapid detection and response when a chemical spill has occurred.

• KSCE Journal of Civil and Environmental Engineering Research
• /
• v.35 no.3
• /
• pp.679-690
• /
• 2015

The need to remove the cause of traffic accidents by improving the engineering system for a vehicle and the road in order to minimize the accident hazard. This is likely to cause traffic accident continue to take a large and significant social cost and time to improve the reliability and efficiency of this generally poor road, thereby generating a lot of damage to the national traffic accident caused by improper environmental factors. In order to minimize damage from traffic accidents, the cause of accidents must be eliminated through technological improvements of vehicles and road systems. Generally, it is highly probable that traffic accident occurs more often on roads that lack safety measures, and can only be improved with tremendous time and costs. In particular, traffic accidents at intersections are on the rise due to inappropriate environmental factors, and are causing great losses for the nation as a whole. This study aims to present safety countermeasures against the cause of accidents by developing an intersection Traffic safety evaluation model. It will also diagnose vulnerable traffic points through BPA (Back -propagation algorithm) among artificial neural networks recently investigated in the area of artificial intelligence. Furthermore, it aims to pursue a more efficient traffic safety improvement project in terms of operating signalized intersections and establishing traffic safety policies. As a result of conducting this study, the mean square error approximate between the predicted values and actual measured values of traffic accidents derived from the BPA is estimated to be 3.89. It appeared that the BPA appeared to have excellent traffic safety evaluating abilities compared to the multiple regression model. In other words, The BPA can be effectively utilized in diagnosing and practical establishing transportation policy in the safety of actual signalized intersections.

• Korean Journal of Ecology and Environment
• /
• v.33 no.3 s.91
• /
• pp.260-266
• /
• 2000

Water quality parameters were surveyed in Lake Andong. Turbidity, temperature, secchi disc transparency (SD), phosphorus, nitrogen and chlorophyll a concentration were measured at dam site from July 1993 to December 1998. Minimum transparency in summer was only about 2 meters in 1993 and 1994, but it decreased to about 1 meter in 1997 and 1988. Total phosphorus concentration of the epilimnion increased slightly from $11{\sim}30\;mgP/m^3$ in 1993 to $18{\sim}42\;mgP/m^3$ in 1998. Total nitrogen concentration of the epilimnion decreased slightly from $1.81{\sim}2.96\;mgN/L$ in 1993 to $1.48{\sim}2.57\;mgN/L^3$ in 1998. TN/TP weight ratio decreased from $82{\sim}281$ in 1993 to $21{\sim}143$ in 1998 due to the increase of phosphorus concentration and the decrease of nitrogen concentration. Dissolved inorganic phosphorus concentration and the decrease of nitrogen concentration. Dissolved inorganic phosphorus and nitrate nitrogen concen tration of the epilimnion were in the range of $0.9{\sim}5.3\;mgP/m^3$ and $1.36{\sim}1.68\;mgN/L$, respectively. Chlorophyll a concentration in summer was in the range of $11.0{\sim}19.1\;mg/m^3$ in 1994, 1996 and 1997, but it decreased to $2.3{\sim}6.5\;mg/m^3$ in 1998. Trophic state of Lake Andong can be classified as mesotrophic to eutrophic from TP, TN and chlorophyll a concentration.