• Korean Chemical Engineering Research
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• v.61 no.2
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• pp.247-257
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• 2023

Precise counting of cell number stands in important position within clinical and research laboratories. Conventional methods such as hemocytometer, migration/invasion assay, or automated cell counters have limited in analytical time, cost, and accuracy., which needs an alternative way with time-efficient in-situ approach to broaden the application avenue. Here, we present simple coding-based cell counting method using image analysis tool, freely available image software (ImageJ). Firstly, we encapsulated RFP-expressing bacteria in a droplet using microfluidic device and automatically performed fluorescence image-based analysis for the quantification of cell numbers. Also, time-lapse images were captured for tracking the change of cell numbers in a droplet containing different concentrations of antibiotics. This study confirms that our approach is approximately 15 times faster and provides more accurate number of cells in a droplet than the external analysis program method. We envision that it can be used to the development of high-throughput image-based cell counting analysis.

• KSCE Journal of Civil and Environmental Engineering Research
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• v.28 no.4A
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• pp.439-445
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• 2008

This paper suggests a modified bond reduction coefficient considering the average CFRP (Carbon Fiber Reinforced Polymer) strain concept for the unbonded prestressed CFRP plate strengthening system. The strengthened length and the pure bending length were seen to influence the variation of the strain of unbonded CFRP plate. Therefore, a new bond reduction coefficient considering such effect was suggested. Comparison with the experimental data revealed that the analytic results obtained by considering the proposed bond reduction coefficient were effective in estimating the strain of the unbonded CFRP plate in the CFRP plate prestressing system.

• Journal of Aerospace System Engineering
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• v.17 no.6
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• pp.63-71
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• 2023

In the present study, the effect of pitch amplitude on the unsteady aerodynamics of a NACA 0012 airfoil is numerically investigated. When the frequency ratio is equal to 1.0, airfoil pitching with 20 and 30 degrees of pitch amplitude shows almost small lift generation, but the lift is significantly increased in case of 10-degree pitch amplitude. When the frequency is 0.5, the lift coefficients have large values, and the lift increases with a decrease in pitch amplitude. When the frequency ratio is 1.0, the airfoil generates large thrust. The thrust decreases as the pitch amplitude decreases. When the frequency ratio is 0.5, drag is generated for the 30-degree pitch amplitude, but the thrust is generated for 10-degree pitch amplitude. In future, the effect of heave amplitude on the unsteady aerodynamics of the airfoil will be studied.

• Journal of Chest Surgery
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• v.37 no.9
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• pp.755-761
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• 2004

We evaluated the efficacy of Dor procedure in patients with ischemic left ventricular dysfunction. Material and Method: Between April 1998 and December 2002, 45 patients underwent the Dor procedure con-comitant with coronary artery bypass grafting (CABG). Left ventricular ejection fraction (LVEF) and left ventricular end-diastolic/end-systolic volumes (LVEDV/LVESV) were measured by echocardiography, myocardial SPECT, and cardiac catheterization and angiography performed at the sequence of preoperative, early postoperative, and one year postoperative stage. Result: Cardiopulmonary bypass and aortic clamp times were mean 141$\pm$64, 69$\pm$24 minutes, respectively. Intraaortic balloon pump (IABP) therapy was required in 19 patients (42%; 7 preoperatively, 9 intraoperatively, 3 postoperatively). Operative mortality rate was 2.2% (1/45). Postoperative morbidities were low cardiac output syndrome (12), atrial fibrillation (5), acute renal failure (4), and postoperative bleeding (4). Functional class (NYHA) was improved from classes 2.8 to 1.1 (p < 0,01). When we compared between the preoperative and early postoperative values, LVEF was improved from 32$\pm$9% to 52$\pm$11% (p<0.01). The asynergy portion decreased from 57$\pm$12% to 22$\pm$9%, and LVEDV/LVESV indexes improved from 125$\pm$39 mL/$m^2$, 85$\pm$30 mL/$m^2$ to 66$\pm$23 mL/$m^2$, 32$\pm$16 mL/$m^2$ (p<0.01). Although these changes in volumes were relatively preserved at postoperative one year, the left ventricular volumes showed a tendency to increase. Conclusion: After the Dor procedure for ischemic left ventricular dysfunction, LVEF improvement and left ventricular volume reduction were maintained till postoperative one year. The tendency for left ventricular volume to increase at postoperative one year suggested the requirement of strict medical management.

• Journal of Korean Society of Forest Science
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• v.112 no.2
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• pp.188-194
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• 2023

This study aimed to derive the basic wood density, one of several carbon emission factors, for carbon accounting of bamboo forests in Korea. Bamboo is mainly distributed in Jeollanam-do and Gyeongsangnam-do provinces, and 101 sample trees were selected for each of the three species (Phyllostachys nigra var. henonis, P. bambusoides, and P. pubescens). The basic wood density derivation used the KS F 2098 method. The measurements showed that the basic wood density was 0.83 g/cm³ for P. nigra var. henonis, 0.81 g/cm³ for P. bambusoides, and 0.72 g/cm³ for P. pubescens. However, the bamboo distribution area in Korea is not very large, and P. pubescens grows in one area only. Therefore, the basic wood density that can be applied to bamboo was 0.79 g/cm³. Evaluation of the uncertainty of the extracted basic wood density showed a very low value of 1.61%, which confirmed the reliability of the basic wood density derived from this analysis. The basic wood density, biomass expansion factor, and root-to-shoot ratio were used to calculate the carbon storage capacity of one bamboo plant and expanded to calculate the capacity for a hectare of bamboo. Carbon storage and absorption of bamboo were calculated by applying a carbon-emission factor, such as the basic wood density. These study results are expected to contribute to the carbon-neutral policy and forest management direction in Korea.

• Journal of the Korea Concrete Institute
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• v.16 no.2 s.80
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• pp.155-162
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• 2004

An analytical method to predict the time dependent flexural behavior of composite girder is presented based on sectional analysis. The time dependent constitutive relation accounting for the early-age concrete properties including maturing of elastic modulus, creep and shrinkage is derived in an incremental format by the first order Taylor series expansion. The sectional analysis calculates the axial and curvature strains based on the force and moment equilibriums. The deflection curve of the girder approximated by the quadratic polynomial function is calculated by applying to the proper boundary conditions in the consecutive segments. Numerical applications are made for the 3-span double composite steel box girder which is a composite bridge girder filled with concrete at the bottom of the steel box in the negative moment region. The calculated results are compared with those by finite element analysis results. Close agreement is observed between the two approaches.

• The Journal of the Korean dental association
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• v.22 no.7 s.182
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• pp.621-633
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• 1984

The possibility of expanding the linear range of the Kinesiograph was studied using a nonferromagnetic mechanical positioning device. The magnet was moved in linear steps of 5 mm through three planes parallel to the frame work carrying the sensors within working range of a 3 cm wide by 4 cm deep by 5 cm high three dimensional lattice and a matrix of 693 data points was achieved. For each data point, the three Kinesiograph outputs were associated with the values of actual position. Once three coordinates of observed values were known, actual values could be determined. A computer program was specially written in Fortran to deal with this work. Because each dat point was 5 mm apart from each other, there would be 480 cubes with 8 data points Further refinement of the system is possible using a smaller interval between each data point. In conclusion, a theoretical model was presented which, by means of computer support, would allow the absolute measurement of jaw position over the entire range of functional jaw movements.

• Journal of the Korean Data and Information Science Society
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• v.26 no.6
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• pp.1305-1315
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• 2015

Consider the risk score which is a function of a linear score for the classification models. The AUC optimization method can be applied to estimate the coefficients of linear score. These estimates obtained by this AUC approach method are shown to be better than the maximum likelihood estimators using logistic models under the general situation which does not fit the logistic assumptions. In this work, the VUS and HUM approach methods are suggested by extending AUC approach method for more realistic discrimination and prediction worlds. Some simulation results are obtained with both various distributions of thresholds and three kinds of link functions such as logit, complementary log-log and modified logit functions. It is found that coefficient prediction results by using the VUS and HUM approach methods for multiple categorical classification are equivalent to or better than those by using logistic models with some link functions.

• Journal of the Korean Institute of Intelligent Systems
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• v.19 no.2
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• pp.212-217
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• 2009

There are rule-based learning method and statistic based learning method and so on which represent learning method for hierarchy relation between domain term. In this paper, we propose to leveling and similarity measure using the extended AHP of fuzzy term in Information system. In the proposed method, we extract fuzzy term in document and categorize ontology structure about it and level priority of fuzzy term using the extended AHP for specificity of fuzzy term. the extended AHP integrates multiple decision-maker for weighted value and relative importance of fuzzy term. and compute semantic similarity of fuzzy term using min operation of fuzzy set, dice's coefficient and Min+dice's coefficient method. and determine final alternative fuzzy term. after that compare with three similarity measure. we can see the fact that the proposed method is more definite than classification performance of the conventional methods and will apply in Natural language processing field.

• Journal of Chest Surgery
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• v.30 no.6
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• pp.613-616
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• 1997

We present a case of 58-year-old (tamale with dilated cardiomyopathy(DCMP) in whom we performed left ventricular(LV) remodeling surgery(Batista operation) to reduce the left ventricle diameter and improve left ventricular unction. The patient was admitted September 1996 with heart failure NYHA class IV. There was severe orthopnea and peripheral edema. 2-D echocardiography(Echo) showed DCMP with the ejection fraction(EF) I5%, LV end diastolic dimension(LVEDD) 80mm, mitral regurgitation(MR) grade IV, tricuspid regurgitation ('m) grade ll. Preoperative cardiac output(CO) was 1.5/L/min and cardiac index(Cl) was 1.0 L/min/m2. We proceeded with LV remodeling surgery by resection a part of LV lateral wall between both papillary muscle, from the mitral annulus to the LV apex. Size of resected LV wall was 90 $\times$ 100 $\times$ 15 mm. At the mean time, mitral valve and tricuspid valve were repaired. Postoperative 2-D Echo showed the EF 37%, LVEDD 50 mna, trivial MR, no TR. CO was 3.SL/min and Cl was 2.3 L/min/m2. Her fuctional NYHA class was 1.