• Journal of KIISE:Software and Applications
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• v.31 no.10
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• pp.1332-1344
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• 2004

Upon development of information super-highway and multimedia-related technoiogies in recent years, more efficient technologies to transmit, store and retrieve the multimedia data are required. Among such technologies, firstly, it is common that the semantic-based image retrieval is annotated separately in order to give certain meanings to the image data and the low-level property information that include information about color, texture, and shape Despite the fact that the semantic-based information retrieval has been made by utilizing such vocabulary dictionary as the key words that given, however it brings about a problem that has not yet freed from the limit of the existing keyword-based text information retrieval. The second problem is that it reveals a decreased retrieval performance in the content-based image retrieval system, and is difficult to separate the object from the image that has complex background, and also is difficult to extract an area due to excessive division of those regions. Further, it is difficult to separate the objects from the image that possesses multiple objects in complex scene. To solve the problems, in this paper, I established a content-based retrieval system that can be processed in 5 different steps. The most critical process of those 5 steps is that among RGB images, the one that has the largest and the smallest background are to be extracted. Particularly. I propose the method that extracts the subject as well as the background by using an Image, which has the largest background. Also, to solve the second problem, I propose the method in which multiple objects are separated using RGB channel selection techniques having optimized the excessive division of area by utilizing Watermerge's threshold value with the object separation using the method of RGB channels separation. The tests proved that the methods proposed by me were superior to the existing methods in terms of retrieval performances insomuch as to replace those methods that developed for the purpose of retrieving those complex objects that used to be difficult to retrieve up until now.

• Journal of the Korean Chemical Society
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• v.67 no.2
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• pp.81-88
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• 2023

The contribution of hydrophobic residues to the protein folding reaction was studied by using HubWA variant proteins with I and L to V mutation. Folding kinetics of all V variant proteins was observed to be satisfied by a three-state on-pathway mechanism, U ⇌ I ⇌ N, where U, I, and N represent unfolded, intermediate, and native state, respectively. Three-state folding reaction was quantitatively analyzed and the free energy of folding of each elementary reactions and overall folding reaction, ΔG^o_UI, ΔG^o_IN, and ΔG^o_UN, were obtained. From the ratio of free energy difference between the variant protein and HubWA, ΔΔG^o_UI/ΔΔG^o_UN (ΔΔG^o_UI = ΔG^o_UI (variant protein) - ΔG^o_UI (HubWA) and ΔΔG^o_UN = ΔG^o_UN (variant protein) - ΔG^o_UN(HubWA)), the contribution of hydrophobic residues to HubWA folding was analyzed. The residues which are located in the hydrophobic core between α-helix and β-sheet, I3, I13, L15, I30, L43, I61 and L67, showed ΔΔG^o_UI/ΔΔG^o_UN value of ~0.5 when each of these residues was mutated to V, indicating that these residues form relatively solid hydrophobic core in the intermediate state. Residues located at the end of secondary structures and loop, I23, L69 and I36 showed ΔΔG^o_UI/ΔΔG^o_UN value below 0.4 when each of these residues was mutated to V, indicating that the region containing these residues are loosely formed in the intermediate state. V17A, L50V and L56V showed fairly high ΔΔG^o_UI/ΔΔG^o_UN value of ~0.8. Since L50 and L56 are located in the region containing long loop (residue 46 to 62), it is suggested that the high ΔΔG^o_UI/ΔΔG^o_UN value of these residues prevents the formation of aggregate at the early stage of folding reaction.

• Journal of the Korean Society of Food Science and Nutrition
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• v.12 no.1
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• pp.7-11
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• 1983

The purpose of this study was to prepare low methoxyl pectins (LMPs) by pectinesterase (PE) using waste tangerine peels and to compare the chemical, physical and gelling properties of the prepared pectins with the commercial LMP. The LMPs were prepared by treating albedo with 0.25M $Na_2CO_3$ to activate the PE. PE was then inactivated by heating. The sample was centrifuged and the supernatant was collected. LMP was obtained as precipitate by adding alcohol to the collected supernatant. The amounts of extractible pectins in albedo were 12.71~12.98% on a dry weight basis. Methoxyl contents of LMPs prepared by treating albedo with PE at pH 7.5 for 10min, at pH 8.5 for 10min, 20min and 30min were 5.12%, 4.27%, 3.08%, 1.85% respectively, demonstrating that the methoxyl contents of the preparations decreased as the degree of treatment albedo with PE increased. The acetyl contents of the preparations ranged from 0.09% to 0.12%, the values of which do not interfere with gel formation. The anhydrouronic acid contents of the prepared pectins were in the range of 94.2%~94.8%. The values of viscosity and molecular weights of the prepared LMPs tent to decrease as the degree of PE action on albedo increased. The textural value of the prepared LMP gels determined by Consistometer, Ridgelimeter and Instron denonstrated that the excess treatment of albedo with PE significantly impaired the gelling properties of the preparations.

• Korean Journal of Environmental Agriculture
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• v.19 no.3
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• pp.194-200
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• 2000

The amount of swine wastewater reaches about $197,000m^3$ per day at live-stock houses in the whole country. A half of the swine wastewater resources are too small to be restricted legally. This untreated wastewater causes the eutrophication in the water bodies. In case of swine wastewater treatment, the solid-liquid separation must be performed because feces(solid phase) and urine(liquid phase) have large differences in nitrogen and phosphorus concentration. It is necessary to assess exactly the concentration of the pollutants in swine wastewater for planning the wastewater treatment facilities. A full-scale operation was carried out in K city and the plant is consists of conventional plant, the supplementary flocculation basin of chemical treatment process and $anaerobic{\cdot}aerobic$ basin for nitrogen removal. The improved full-scale swine wastewater treatment plant removed the $1,500{\sim}3,000mg/l$ of total-nitrogen(T-N) to 120mg/l of T-N and $131{\sim}156mg/l$ of total-phosphorus(T-P) to $0.15{\sim}1.00mg/l$ of T-N. Accordingly, as a results of operational improvement, the removal efficiencies of T-N and T-P were over $92{\sim}96%$, 99%, respectively. The continuous supply of organic carbon sources and the state of pH played important roles for the harmonious metabolism in anaerobic basin and the pH value of anaerobic basin maintained at about 9.0 for the period of the study.

• Journal of the Mineralogical Society of Korea
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• v.16 no.1
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• pp.33-47
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• 2003

Various applied-mineralogical characterization including measurements of surface area, size distribution, swelling index, and viscosity were done for some domestic bentonites in order to decipher the rheological properties and their controlling factors. The bentonites, which are Ca-type and relatively low-grade (rnontmorillonite contents: 30 ∼ 75 wt%), occur mostly as subhedral lamellas with the size range of 2 ∼ 4 $\mu\textrm{m}$. The size distribution of mineral fractions in bentonite suspension is dominant in the range of 10 ∼ 100 $\mu\textrm{m}$, and though rather complicated, exhibits roughly bimodal patterns. The feature is more conspicuous in the case of zeolitic bentonite. The bentonites have surface areas ranging 269 ∼ 735 $\m^2$/g, which are measured by EGME adsorption method. The EGME surface areas are nearly proportional to the rnontmorillonite contents, moisture contents, or total CEC. In the surface area measurements, zeolitic bentonites have slightly higher values than those zeolite- free types. The measured swelling index and viscosity of domestic bentonites are comparatively low in values. The swelling values of bentonites were measured to be 250∼500% at maximum by progressively mixing amounts of 2 ∼ 5 wt% Na$_2$CO$_3$, which varies depending on the contents of rnontmorillonite and other impurities, especially zeolite. Much amount of sodium carbonate is required for optimum swelling property of zeolitic bentonited which has usually strong Na- exchanged capacity. The bentonites, which are comparatively feldspar-rich and low in size and crystallinity, tend to be higher in viscosity values. Tn addition, the viscosity is largely higher in case of the bentonites with higher pH in suspension. However, the rheological properties of bentonites such as swelling index and viscosity do not show any obvious relationships with rnontmorillonite contents and mean particle size in suspension. In contrast, roughly speaking, the swelling index of bentonites is reversely proportional to the values of surface area which can be regarded as a collective physico-chemical parameter encompassing all the effects caused by mineral composition, surface charge, particle size, morphological farm, and etc. in bentonites. Thus, the rheological properties in bentonite suspension appear to be rather complicated characteristics which mainly depend on the flocculation of clay particles and the mode of particle association, i.e. quasicrystals, controlled by surface charge, morphology, size, and texture of rnon-tmorillonite, and which partly affected by the finer impurities such as zeolite.

• Korean Journal of Soil Science and Fertilizer
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• v.37 no.2
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• pp.66-73
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• 2004

As one of the typical farming practices in the sloped upland in Pyeongchang and Hongcheon area, application of piling with coarse saprolite materials has been practiced by farmers for several reasons such as reduction of damage by monocropping, better development of plant roots, and better drainage. However, adverse effect on application of coarse saprolite soil materials to environmental aspects should not be ignored. Therefore, this research was conducted to evaluate the physicochemical properties of coarse saprolite materials in upland fields in Pyeongchang area. According to particle size distribution of coarse saprolite materials, averaged gravel contents for Pyeongchang and Hongcheon county were 16.7 and 25.3%, respectively. There was no significant difference in gravel contents by soil depth, and CV values for each particle size ranged from 20 to 40%, which implied that application of coarse material with similar properties. When we compared CEC values of dressed soil with or without considering gravel content, CEC values decreased as increasing gravel contents. The penetration resistances were 0.04-7.48 MPa at the 0 to 10 cm surface soil, and 0.10 to 8.80 MPa at the depth below 11 cm. The bulk density of the soil was $1.15g\;cm^{-3}$ at the surface soil and 1.29 to $1.35g\;cm^{-3}$ at the soil depth below 10 cm. The organic matter content, cation exchange capacity, and avaliable $P_2O_5$ concentrations of soil in upland where piling with saprolite materials of Pyeongchang area applied were $12.4g\;kg^{-1}$, $7.1cmol_c\;kg^{-1}$, and $526mg\;kg^{-1}$, respectively. Cation exchange capacity was lower than that of averaged Korean upland soil, while available $P_2O_5$ concentration was relatively higher than that of averaged Korean upland, which indicated high input of various fertilizers.

• Clinical and Experimental Reproductive Medicine
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• v.37 no.2
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• pp.99-113
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• 2010

Objective: It has been recently reported that very small stem cells with pluripotency are detected in murine and human. The purposes of this study are to confirm whether very small putative stem cells (VSPSCs), which have the proper characteristics of stem cells as well as the expression of stem cell markers, are detected in human endometrium. Methods: The endometrial cells of 5 women, which were obtained by endometrial biopsy, were cultured for 2 weeks and were confirmed for the expressions of alkaline phosphatase, OCT-4 and CXCR4 by immunochemistry. Subsequently VSPSCs were separated by percoll density gradient method and were cultured. Also VSPSCs and their derived cells were confirmed for the expressions of OCT-4 and CXCR4. Results: The colonies, which is composed with VSPSCs less than 3 ${\mu}m$ and the 5~15 ${\mu}m$ sized hyperchromatic round cells, were detected in the endometrium of all of women and showed the strong expressions of alkaline phosphatase, OCT-4 and CXCR4. In culture after the separation of VSPSCs by percoll, these cells showed the morphological and functional characteristics of stem cells; self-renewal, colony formation, embryoid body-like formation and differential plasticity. VSPSCs formed gradually the 5~15 ${\mu}m$ sized hyperchromatic round cells and the 10~20 ${\mu}m$ sized sphere-shaped cells by cell-to-cell aggregation or cell fusion. Then these cells differentiated the various cells including fibroblast-like cells, nerve-like cells and endothelium-like cells. VSPSCs and their derived cells often showed the strong expressions of OCT-4 and CXCR4. Conclusion: VSPSCs less than 3 ${\mu}m$ and their derived cells are detected in human endometrium and these cells have the proper characteristics of stem cells and the expressions of stem cell markers as alkaline phosphatase, OCT-4 and CXCR4.

• Korean Journal of Ecology and Environment
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• v.38 no.3 s.113
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• pp.372-381
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• 2005

This study was conducted to test the efficiency of water quality improvement using the dissolved air flotation (DAF) technique in a shallow eutrophic reservoir. The application of DAF was followed by the addition of a chemical coagulant (poly aluminum chloride; PAC). The experiment was conducted in the mesocosm scale (wide ${\times}$ length ${\times}$ depth: 6 m ${\times}$ 6 m ${\times}$ 3 m). Suspended solids (SS) and volatile SS (VSS) concentration decreased by 54 ${\sim}$ 71% and 57 ${\sim}$ 79% of the initial concentrations, respectively. Total phosphorus and Chl- a concentration also decreased by 74 ${\sim}$ 92% and 54 ${\sim}$ 98%, respectively. BOD decreased by>86% while COD decrease ranged 29 ${\sim}$ 63%. Dissolved inorganic P (DIP) and dissolved total P (DTP) concentration decreased by 34 ${\sim}$ 88% and 62 ${\sim}$ 88%, respectively. After DAF application further onto the sediment, DIP-release rates from the sediment decreased by 17% (0.82 ${\to}$ 0.68 mg $m^{-2}$$day^{-1}$ in the oxic condition and 23% (2.27 ${\to}$ 1.76 mg $m^{-2}$$day^{-1}$) in the anoxic condition, compared to the release rate from the untreated sediment. DTP-release rate from both the oxic and anoxic sediments also decreased by 33% (5.62 ${\to}$ 3.78 mg $m^{-2}$$day^{-1}$) and 20% (6.23 ${\to}$ 4.99 mg $m^{-2}$$day^{-1}$), respectively. These results suggest that the DAF application both to the water column and onto the sediment be effective to improve water quality by removing particulate matters in the water column as well as reducing P-release from the sediment.

• Journal of the Society of Cosmetic Scientists of Korea
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• v.35 no.4
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• pp.301-307
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• 2009

In the previous study, we evaluated and reported about the anti-oxidative activities of extract/fraction of Castanea crenata leaf. Extract/fraction of Castanea crenata leaf showed excellent free radical scavenging activity, cell protective activity and inhibitory activity on tyrosinase and elastase. In this study, in order to investigate the stability of cream containing 0.2 % Castanea crenata ethyl acetate fraction. pH, viscosity, and absorbance were measured under 4 different temperature ($4^{\circ}C$, $20^{\circ}C$, $37^{\circ}C$, $45^{\circ}C)$ and under the sun light at 2 weeks intervals for the 8 weeks. The variations on pH and viscosity of all experimental creams were similar to control cream. The absorbance variation of extract from experimental cream at 353 nm was in the order: under the sun > $45^{\circ}C$ > $37^{\circ}C$ > $20^{\circ}C$ > $4^{\circ}C$. It shows that ethyl acetate fraction in the cream can be oxidized under the sun. The bad smell and discoloration were not shown. Also, physical changes as creaming and cohesion were not shown. Also, transepidermal water loss (TEWL) and water contents in skin were measured. The cream containing Castanea cranata leaf extract was applied to the right lower arm. After 120 min, TEWL of parts was decreased as 29.7 % (experimental cream) and 5.4 % (control cream) respectively. And the water contents in skin were increased 22.6 % (experimental cream) and 24.7 % (control cream) respectively. It was confirmed that a cream containing ethyl acetate fraction of Castanea crenata leaf shows the superior moisturizing effect. The results showed that Castanea crenata leaf extract could be used as a new active ingredient for anti-aging cosmeceuticals.

• Journal of the Korea Academia-Industrial cooperation Society
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• v.19 no.9
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• pp.279-287
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• 2018

Sealants are an important element of modern architecture and serve as a building protection against weathering by providing barriers against ingress of moisture, air, and other materials. Exposure to a variety of environments often reduces lifespan due to changes in physical, chemical and mechanical characteristics, and UV, humidity, and temperature expansion are important issues that are directly related to durability. In this study, a combined deterioration test chamber was developed to simulate the environment of the open air as an instrument for verifying the durability of structural sealing materials indoors. In order to replicate special weather conditions, such as yellow dust, acid rain, and contamination by microorganisms, it was deemed impossible to replicate the outdoor environment by 100 %, and the results of the results of the results of the external exposure test of the structural sealant and the combined deterioration testing device. As a result of the displacement test of the outdoor exposure test, it was determined that the sealant was breaking apart and that it would be smooth, and the displacement would be up to three times greater than the initial material value of 1 year. The displacement test results of the combined deterioration test device show the tendency to deteriorate, decreasing the elasticity and tensile characteristics. In the case of denatured silicon, the current 400 cycles have been completed to confirm 12 months of degradation of the external exposure. The deformation of the test specimen cannot be verified with the naked eye, so it is considered that the conditions of the specimen are more stable than the silicon sealant. As a result of the outdoor exposure test, if the combined deterioration test device is structured and proposed in the relevant guidance or specification, the anticipated lifespan of 12 months in the actual use environment can be verified indoors and below 3 months later, economically.