• Proceedings of the Korean Society of Applied Pharmacology
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• 1994.04a
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• pp.211-211
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• 1994

항종양성 화합물의 개발을 지향한 해양생물 유래의 생물활성 물질의 화학적 연구의 일환으로써 다양한 종류의 생물활성 (즉, 항균작용, 혈중콜레스테롤 저하작용)등을 나타내며, 구입수가 용이한 홍조 꼬시래기 (Gracilaria verrucosa) 및 갈조 지충이 (Sargassum thunbergii)의 아세톤 및 메탄올 엑스를 EtOAc 및 n-BuOH로 분획한후 각 분획을 SiO$_2$ column, TSK gel(Toyo pearl HW-40F), $\mu$-Bondapak column 및 HPLC등으로 분리 정제하여, 새로운 glyceroglycolipid(GV-5, -6, ST-6) 및 glycerylglycoside(GV-12)를 얻었다. 이렇게 분리된 화합물의 물리화학적 성질, 화학반응 및 분광학적 data를 종합 검토한 결과 GV-5, -6, -12, 및 ST-6은 각각 1,2-diacyl-3-0-($\alpha$-D-galactopyranosyl) glycerol (acyl : palmitate-oleate-arachidonate 4: 1 : 9) (1), 1,2-diacyl -3-0-〔$\alpha$-D-galactopyranosyl -(1 "$\longrightarrow$6')-0-$\beta$-D-galactopyranosyl〕 glycerol (acyl : palmitate-oleate-arachidonate(5:1:4)(3), 2-0-($\alpha$-D-galactopyranosyl) glycerol (5), 및 sodium salt of 1-acyl-3-0-(6'-sulfo-$\alpha$-D-quinovopyranosyl) glycerol(acyl:palmitate-oleate 96:4)(8) 이라는 것이 판명되었다. 그리고 GV-5 및 -6은 마우스 백혈병세포 (L1210)에 대한 세포독성 ($IC_{50}$/ of GV-5, and -6:8.0$\mu\textrm{g}$/ml and 10.8$\mu\textrm{g}$/ml )외에, 사람의 상피암세포에 대한 성장억제효과 [growth inhibitor(%) in 20$\mu\textrm{g}$/m1 :GV-5(39.9%), GV-6(16.7%)를 나타내었으며, 한편, GV-5, GV-6, -12 및 ST-6은 각각 쥐의 F9 기형암종 세포의 분화유도 활성을 나타내어 항종양제로의 개발에 많은 흥미가 기대된다.기대된다.

• The Korean Journal of Ecology
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• v.27 no.3
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• pp.173-177
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• 2004

The number and abundance of epiphytic algae growing on Sargassum thunbergii and their functional forms were examined. Thalli of S. thunbenii were collected on the nine islands of southern coast and at Gyeokpo on the western coast of Korea in summer 2001. A total of 25 species, 6 green (24%), 2 brown (8%), 17 red algae (68%) were identified in the present study. Caulacanthus okamurae, Ulva pertusa, Sphacelaria furcigera were found on the thalli of S. thunbegii that collected at the all regions. Caulacanthus okamurae, Champia bifida and Laurencia venusta grew on the lower parts of S. thunbergii thalli whereas, Polysiphonia spp. attached to the apical parts of the plants. Four functional forms, such as sheet-form (8%), filamentous-form (52%), coarsely branched-form (32%) and articulated-calcareous algal form (8%) were distinguished. The epiphytic algae on the thalli of S. thunbergii are mainly annual opportunistic algae, filamentous-form algae and smaller red algae. Also, most epiphytic seaweeds of S. thunbergii produce and release spores before pseudoperennial host plants are necrotic in late autumn.

• Microbiology and Biotechnology Letters
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• v.44 no.1
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• pp.48-54
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• 2016

In this study, biosugar production by the red-algae Eucheuma cottonii was investigated using dilute sulfuric acid-catalyzed hydrolysis and data analysis by response surface methodology. This approach yielded 25.8 g/l total reducing sugar under the conditions of $160.1^{\circ}C$, 1% (v/v) sulfuric acid, and 13.1 min. The sugar concentration showed a linear inverse correlation with the combined severity factor (CSF) of the reaction conditions. CSF was calculated as $log(t{\cdot}e{xp}[(T_H-T_R)/14.75])-pH$, where t is the coupling reaction time, $T_H$ is the target temperature, and $T_R$ is the reference temperature ($100^{\circ}C$). In addition, levulinic acid production showed a linear positive correlation with CSF. E. cottonii may represent a useful feedstock for sugar production in the field of bioenergy.

• Korean Journal of Fisheries and Aquatic Sciences
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• v.30 no.2
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• pp.291-296
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• 1997

The dietary fiber contents of seaweeds including Drown algae (Undaria pinnatifida, Laminaria japonica, Hizikia fusiforme), red algae (Gelidium amansii, Gracilaria verrucosa) and green algae (Ulva lactuca) and the extraction condition of the fiber was investigated. The dietary fiber contents of Undaria pinnatifida, Laminaria japonica, Hizikia jusiforme, Gelidium amansii, Gracilaria verrucosa and Ulva lactuca were $47.2\%,\;50.7,\;42.6\%,\;48.8\%,\;44.5\%\;and\;40.0\%$, respectively. It was effective to extract soluble dietary fiber with sodium salts such as disodium carbonate and disodium EDTA in brown algae. In red algae, it was effective to extract soluble dietary fibre with disodium EDTA. Also, the extraction time and temperature affected the yields of dietary fiber.

• Korean Journal of Fisheries and Aquatic Sciences
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• v.16 no.4
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• pp.368-378
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• 1983

The community structure of intertidal benthic marine algae were studied seasonally at Odongdo, southern coast of Korea, from June 1982 to May 1983. Algal coverage in $50{\times}50\;cm$ quadrat were recorded for each species by line transect method. The vertical zonation investigated by line transects is recognized into three groups : Upper, middle, and lower zones. The representative species are Gelidium divaricatum, Enteromorpha linza, Porphyra yezoensis, Scytosiphon lomentaria, Blidingia nana, Ectocarpus confervoides in the upper, Ulva pertusa, Chondria crassicaulis in the middle, and Sargassum sagamianum, S. thunbergii, Undaria pinnatifida, Gelidium amansii and various other red algae in the lower zone. The number of algal species and coverage were generally highest in April and lowest in August. Species which appear dominant at least once a year were all of the spring type and the others were autumn type. According to the cluster analysis by similarity index community coefficient(SICC) among 5 transects, the algal communities are divided into two groups, i. e. open-sea group and inland-sea group.

• The Journal of Korean Medicine
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• v.29 no.4
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• pp.180-193
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• 2008

Objectives: We intended to identify factors related to the severity of hot flush of climacteric women from an East-West medical point of view. Methods: We surveyed 446 climacteric women who had received Korean oriental medical questionnaires about clinical symptom patterns and health medical examinations at Kyung-Hee University Medical Center over 1 year, from June 2007 to May 2008. Then, we compared hot flush with clinical symptom pattern and health medical examination result. Results: As the severity of hot flush increased, hypnagogic disorder in sleep pattern, abdominal gaseous distention in digestion pattern, tenesmus in evacuation pattern, yellow or reddish urine in voiding pattern, spontaneous sweating in sweating pattern, chest oppression in psychologic pattern, not-pulling-bedclothes in cold-heat pattern and mouth dryness in craniocervical symptom increased (p<0.05). In relation to digestion pattern, the severity of hot flush showed statistical significance according to prevalence of gastritis diagnosed by gastroscopy and upper GI series(p<0.01). In relation to voiding pattern, the severity of hot flush showed statistical significance according to prevalence of urine protein diagnosed by urinalysis (p<0.05). In relation to sweating, psychologic & cold-heat pattern, triiodothyronine (T3) increase and thyroid stimulating hormone (TSH) decrease were significantly correlated as the severity of hot flush increased (p<0.01). Conclusions: The result showed that hot flush of climacteric women had to be considered in respect of digestion disorder related to gastritis and sweating psychologic cold-heat disorder related to thyroid hormone.

• Journal of the Korean Applied Science and Technology
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• v.11 no.1
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• pp.79-85
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• 1994

All the activities and physical properties including surface tension' foaming power, foam stability, emulsifying power, dispersion effect of 3-(N, N-dimethyl N-alkylammonio)-2-hydroxyY-1-propane sulfonate (HSB)류와 3-CN-alkylamidopropyl-N,N-dimethylamm-onjo)-2-hydroxy-1-propane sulfonate (APSB) aquous solution were measured and critical micelle concentration was evaluated. Their cmc of hydroxy sulfobetaine derivatives evaluated by the surface tension method was $1.0{\times}10^{-3}{\sim}1.0{\times}10^{-4}$ mol/l, and surface tension of the aquous solution was decreased to $27{\sim}38dyne/cm$. The experimental results for foaming power, foam stability, emulsifying power in liquid paraffin showed a good surface active properties, especially, dispersion effect in ferric oxide exhibited some efficient surface active properties, and then it would be expected to application as detergent and dispersion agent.

• Korean Journal of Fisheries and Aquatic Sciences
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• v.35 no.1
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• pp.64-70
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• 2002

The marine algal flora and community structure of Gahakri in Jindo Gun, southern coast of Korea, was investigated monthly during September, 1993-August, 1994. As the results, a total of 87 species including 11 greens, 15 browns and 61 reds was identified. The marine algal flora was classified as the mixed flora based on the species composition. The dominant species of algal community throughout the year was Gloiopeltis furcata, and the subdominants were Sargassum thunbegii, Ulva pertusa and Hizikia fusiformis. In their vertical distribution, the species of algal community distributed yearly (or seasonally) from upper to lower intertidal zones were Gloiopeltis furcata-Corallina pilulifera, Myelophycus simplex, Gloiopeltis tenax (May$\~$October), Dumontia simplex, Sargassum thunbergii-Hizikia fusifomis, Ulva pertusa, Chondracanthus intemedius, Carpopeltis affinis.

• Korean Journal of Fisheries and Aquatic Sciences
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• v.47 no.1
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• pp.45-51
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• 2014

Scuticociliates are regarded as serious pathogens in marine aquaculture worldwide. In Korea, they cause mass-mortalities in fish such as the commercially important olive flounder Paralichthys olivaceus. In particular, mixed infections of scuticociliates with pathogenic bacteria have been commonly reported. During efforts to identify natural marine-algae derived products that possess anti-bacterial and anti-scuticociliate properties, we found that an 80% methanolic extract of the red alga Polysiphonia morrowii Harvey exhibits both anti-scuticociliate activity against Miamiensis avidus, which is a major causative agent of scuticociliatosis, and anti-bacterial activities against fish pathogenic bacteria. Activity-guided fractionation and isolation of the 80% methanolic extract of P. morrowii yielded three bromophenols, which were identified as 3-bromo-4,5-dihydroxybenzyl methyl ether (1), 3-bromo-4,5-dihydroxybenzaldehyde (2) and urceolatol (3) based on spectroscopic analyses. 3-bromo-4,5-dihydroxybenzyl methyl ether (1) showed the highest anti-bacterial and anti-scuticociliate activities, with a minimal inhibitory concentration (MIC) of $62.5{\mu}g/mL$ (against Vibrio anguillarum) and minimal lethal concentration (MLC) of 62.5 ppm (in seawater). Investigations of the anti-bacterial and anti-scuticociliate activities of seventeen bromophenol derivatives, including the three isolated natural bromophenols, showed that the existence of an electron donating group or atom with a non-covalent electron pair at $C_4$ of the 2-bromophenol structure may be important in anti-scuticociliate activity. These findings suggest that the extract and bromophenol derivatives of P. morrowii may provide useful alternatives in aquaculture anti-scuticociliate therapies.

• Journal of the Korean Society of Food Science and Nutrition
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• v.25 no.6
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• pp.1069-1073
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• 1996

Inhibition of xanthine oxidase by seaweed extracts obtained from Undaria pinnatifida, Ecklonia stolonifera, Ecklonia cava, Laminaria japonica, Sargassum, Codiumfragile, Enteromorpha compressa and Porphyra tenera were investigated. Extracts of E. stolonifera and E. mua remarkably inhibited xanthine oxidase activity compared to those of other seaweed. The xanthine oxidase inhibitory activity of E. cava was higher than that of E. stolonifera. Diethyl ether extract from E. cava was more effective in the inhibition of xanthine oxidase than other solvent extracts. Two xanthine oxidase inhibitors(A-1 and A-2) from diethyl ether extract were isolated and purified by silica gel column chromatography, thin layer chromatography and high performance liquid chromatography. Xanthine oxidase inhibitory activities of these compounds were 27.8 and 48.1% per 0.4mg, respectively. The active compound A-2 had absorption peak at 420nm, 456nm and 467nm, which can be considered as siphonaxanthine.