• Korean Journal of Clinical Laboratory Science
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• v.52 no.3
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• pp.221-228
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• 2020

Ochrobactrum anthropi is a non-fermentative oxidative gram-negative bacillus that produces oxidase. Distinguishing a mixed culture with non-fermenting bacteria having a similar appearance and oxidase-positive is difficult, and there is a limit to accurate identification with a biochemical identification system. This paper proposes that the Matrix-Assisted Laser Desorption/Ionization Time-of-Flight Mass Spectrometry Platform (MALDI-TOF) method is useful for classifying bacteria that are difficult to identify using biochemical testing methods. As a result of analyzing five cases of O. anthropi examined using MicroScan, it took 6.5 days to the final report, which was 3.5 days more than the 3.0 days of E. coli. The pus sample in patient 5 was a mixed infection with Achromobacter xylosoxidans, and it took 11.3 days because of multiple subculture and retests. Four patients were over 60 years old with an underlying disease, and the possibility of opportunistic and nosocomial infections could not be excluded. Among them, samples collected after 92 days of hospitalization were resistant to imipenem and meropenem. Therefore, an examination using the MALDI-TOF method will be useful for the rapid and adequate treatment of patients with difficult identification, such as O. anthropi.

• Microbiology and Biotechnology Letters
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• v.50 no.3
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• pp.347-353
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• 2022

In this study, the effect of a synbiotic mixture of probiotics and 3% inulin on the growth, non-specific immune response, and mortality due to disease resistance of Paralichthys olivaceus was investigated. The results revealed that an 8-week diet of synbiotic-added feed, had no significant effect on the growth of P. olivaceus, Analysis of the hematological parameters revealed that there was a significant difference in the content of AST, total protein, and cholesterol among the groups, whereas there was no significant difference in the ALT and glucose contents. Further, there was no significant difference in the lysozyme activity of the synbiotic and control group after 8 weeks' feeding. In addition, when the symbiotic mixture was used rather than probiotics alone, the NBT activity of the synbiotic group increased compared to that of the control group. Moreover, when only probiotics were used, the cytokine expression in the spleen of the samples was significantly lower than that in the control group, whereas the expression was significantly higher in the spleen of the synbiotics group. However, there was no significant difference in the cytokine expression in the liver, intestine, and kidney of the three groups. Lastly, after injection for 2 weeks, the mortality rates of the control group towards Edwardsiella tarda, Streptococcus parauberis, and Streptococcus iniae were 95, 85 and 85%, respectively; those of the probiotics group were 85, 80, and 80%, respectively; and those of the synbiotics group were 80, 80, and 85%, respectively.

• Journal of Veterinary Clinics
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• v.20 no.2
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• pp.242-244
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• 2003

A 3-year-old, male, mongrel dog was presented with pruritus, alopecia to the Veterinary medical Teaching Hospital, College of Veterinary Medicine of Kyungpook National University. On physical examination, generalized erythema, papules, scales and crusts were observed. The canine scabies infestation was diagnosed by deep skin scraping and Malassezia spp. and Staphylococcus spp. were found on skin cytology. He was treated with selamectin at 6 mg/kg applied to the skin in a single spot every two weeks for one month in order to investigate the effect of selamectin on canine scabies and was treated with amoxicillin trihydrate/clavulanate pattassium, ketoconazole and chlorhexidine shampoo for Malassezia sup. and Staphylococcus spp. simultaneously. When he was rechecked after 7, 14, 21, and 28 days after the first application of selamectin, scabies werent seen. The dermatological signs including pruritus, papules, scales, and crusts were remarkably resolved at 14 days. The alopecia was almost disappeared and few Malassezia spp. and Staphylococcus spp. were seen after 28 days. The efficacy of selamectin by this protocol is excellent in the dog infestated canine scabies.

• Journal of the korean academy of Pediatric Dentistry
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• v.32 no.1
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• pp.126-131
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• 2005

The purpose of this study was to observe the potentiality of a mixture of antibiotics to relieve the endodontic and periapical infections in primary teeth. A mixture of metronidazole, ciprofloxacin, and minocyclin in distilled water or in a root canal sealer were used to disinfect the infected endodontic and periapical lesions. For application of this mixture, medication cavities were prepared at orifices of root canals by using the $Fissurotomy^{(R)}$ burs(S S White, USA). The cavities and bottom of pulp chamber were filled with antibiotics mixture. In this cases, clinical symptoms were improved following a single application of that mixture. Gingival swelling, pain, and fistulae were disappeared within a week. More long term evaluation will be needed. Although there were no reports of side effects, care should be taken if children are sensitive to antibiotics. Within the limitations of this case, the mixture of 3 antibiotics provided an excellent improvement in treatment of infected primary teeth under the physiologic root resorption.

• Korean Journal of Veterinary Research
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• v.1 no.1
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• pp.72.1-72.1
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• 1961

Pasteurella multocida에 대(對)한 각종성장(各種性狀) 및 균주(菌株) 상호간(相互間)의 이동(異同)에 관(關)하여는 많은 연구(硏究)가 있으나 아직까지의 문헌(文獻)에 의(依)하면 자연감염(自然感染에)에 있어서는 일반적(一般的)으로 동종동물(同種動物)에 감염(感染)한다 하여도 각종동물(各種動物)에서 분리(分離)된 Pasteurella multocida는 그 병원성(病原性)이 분리(分離)된 동물(動物)과 명확(明確)한 상관관계(相關關係)를 가지는것이 아니며 그저 생물학적(生物學的) 내지(乃至) 생화학성장(生化學性狀) 혹(或)은 면역학적성장(免疫學的性狀)에 있어서 그차이(差異)로 균형(菌型)을 분류(分類)할수 있는데 불과(不過)하다. 그런데 월지(越智)는 그의 분류(分類)에서 A형균(型菌)(조류(鳥類)에서 분리(分離)한 균주(菌株))은 가토(家兎) mouse 및 조류(鳥類)에 병원성(病原性)이 있고 타(他) 포유동물(哺乳動物)에 병원성(病原性)이 없다고 하였으며 또 B. C. D형균(型菌)(포유류(哺乳類))에서 분리(分離)한것)은 닭, 가압등(家鴨等) 조류(鳥類)에 병원성(病原性)이 없다고 하였다. 그러나 필자(筆者)는 출혈성패혈증(出血性敗血症)으로 죽은 오리 및 돈(豚) cholera와 혼합감염(混合感染)을 이르킨 돈(豚)으로부터 각각(各各) 따로 Pasteurella multocida를 분리(分離)하여 이 병균(病菌)를 가지고 각종동물(各種動物)에 접종시험(接種試驗)을 한바 상술(上述)한 월지(越智)의 견해(見解)는 부합(附合)되지 않았다. 즉 이 양주(兩株)는 돈(豚), 가토(家兎), 오리, 닭등(等)에 대(對)하여 동양(同樣)으로 강(强)한 병원성(病原性)을 가졌으며 또 양주(兩株)는 다같이 면양(緬羊)에 대(對)하여 거이 병원성(病原性)이 없고 Guinea Pig은 양주(兩株)에 대(對)한 저항성(抵抗性)이 다같이 강(强)하였다. 다시말하면 이 양주(兩株)는 조류(鳥類)나 포유류(哺乳類)에 대(對)하여 거의 동일(同一)한 병원성(病原性)이 있음을 인정(認定)하였다.

• Korean Journal of Ichthyology
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• v.27 no.4
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• pp.306-309
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• 2015

In 2014, high mortality was observed in olive flounder (Paralichthys olivaceus) during experimental infection. Diseased fish did not show any outward clinical signs, but numerous fungal hyphae were detected in the gills. No parasites or bacteria were isolated from the diseased fish. A high mortality rate (100%) resulted when cohabitation with fungi-infected fish were applied to healthy fish. The affected fish exhibited sever fungi infection in the gills. Histopathological examination revealed numerous fungal hyphae and mycelium around gill filament, lamellar and racker. These results suggest that the fungi may be related with the mortality of olive flounder.

• Biomedical Science Letters
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• v.2 no.1
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• pp.31-40
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• 1996

Escherichia coli is one of the most common etiological agents in urinary tract infection. An important virulence factor is the adhesive capacity of E. coli to uroepithelial cell, mediated by bacterial fimbriae. The Adhesion property has been regarded as an important virulence determinant in urinary tract infections. A total of 60 patients, who were diagnosed microbiologically as urinary tract infections, were examined by immunoblotting and enzyme-linked immunosorbent assay(ELISA). Uropathogenic E. coli with recombinant plasmid were positive for mannose resistant hemagglutination (MRHA). For identification of p-fimbriae subtype in uropathogenic E. coli, In the immunoblot analysis, specific bands in the range of p-fimbriae molecular weight of 17KD-22KD were identified. For the distribution of p-fimbriae subtype in the patient sera, 34/60(56.7%) were positive for $F7_1$, 28/60(46.7%) were positive for $F7_2$, and 30/60(50%) were positive for F13 with immunoblotting method. similar trends were observed in the enzyme-linked immunosorbent assay. Relatively good specificity(92.6%) and sensitivity(90%) were found in the ELISA test system using mixed antigens of purified $F7_1$, $F7_2$, and F13 p-fimbriae, and 60 sera from patients with urinary tract infections. In conclusion The serological tests were convenient method in diagnosis of urinary tract infections. among those ELISA could be recommended in diagnosis of urinary tract infections.

• Investigative Magnetic Resonance Imaging
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• v.6 no.2
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• pp.152-157
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• 2002

Purpose : The differential diagnosis between Modic type I degenerative spine and infectious spondylitis sometimes is difficult, because the affected bone marrows in both disease show similar signal intensity on conventional MR imaging. We evaluate the usefulness of diffusion-wighted MR imaging for differential diagnosis between Modic type I degenerative spine and infectious spondylitis. Materials and methods : The spin-echo and diffusion-weighted MR images of eight patients with Modic type I degenerative spines and 14 patients with infectious spondylitis diagnosed by clinical findings or CT-guided biopsies we re analyzed. The diffusion-weighted imaging sequence was based on reversed fast imaging with steady-state precession (PSIF). Signal intensity changes of the vertebral bone marrow on conventional spin-echo and diffusion-weighted MR imaging were compared between degenerative spine and infectious spondylitis. Results : On T1-weighte d images, the affeted bone marrow in both disease showed hypointense signals. On T 2-weighted images, all of type I degenerative spine and 11 of infectious spondylitis showed hyperintensity, and three of infectious spondylitis showed heterogeneo us mixed signal intensity. On diffusion-weighted MR images, all of type I degenerative spine were hypointense with peripheral high signal intensity to normal vertebral body, but infectious spondylitis was hyperintense (n = 11) and hypointense (n=3). Conclusion : Diffusion-weighted MR imaging is useful to differentiate Modic type I degenerative spine from infectious spondylitis. On diffusion-weighted images, the high singal intensity of bone marrow suggests infectious spondylitis, whereas the low signal intensity of bone marrow with peripheral focal high signal intensity suggests type I degenerative spine.

• Korean Journal of Veterinary Research
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• v.41 no.2
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• pp.211-218
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• 2001

The $M_r$ 63,000 glycoprotein (GP63) and lipophosphoglycan (LPG) of Leishmania donovani were evaluated as vaccine candidates against visceral leishmaniasis. Mice were immunized with liposomeencapsulated GP63 and/or LPG that were purified from the soluble extract of L. donovani promastigotes, and were challenged with virulent amastigotes. Mice immunized with GP63/LPG in liposomes plus BCG resulted in a 27.4% reduction of amastigotes in the liver compared to the control group (liposomes plus BCG), and mice immunized with liposome-GP63 plus BCG failed to induce a protective immune response against the challenge infection. Immunization of mice with GP63 fused to the Schistosoma japonicum glutathione S-transferase (GP63-GST) plus BCG also failed to elicit protective immunity. To analyze the cause of failure to induce protection, cytokine release from the spleen cells of immunized mice and Leishmania-specific serum antibodies were analyzed. Spleen cells from mice immunized with GP63-GST plus BCG that were exposed to soluble extract of L. donovani in vitro produced 10-fold greater quantities of IFN-gamma and 3-fold greater quantities of IL-5 than cells from mice receiving BCG only or saline. Western blot analysis revealed that sera from these mice had Leishmania-specific antibodies recognizing 1 to 3 antigens of L. donovani with M. W. of 60-65 kDa. Although immunization of mice with GP63-GST induced a strong Th1 response, this study indicated that GP63-GST simultaneously elicited the Th2 response of the CD4+ T-cell, which was known to abrogate the protective immune response conferred by the Th1 effector function.

• Proceedings of the Korean Society of Broadcast Engineers Conference
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• fall
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• pp.62-64
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• 2021

최근 코로나 19의 확산으로 인하여 코로나 감염으로 인한 외부 방송 촬영에 제약이 생기면서, 제작환경은 실외에서 실내의 가상 환경 제작 수요가 크게 증가하고 있다. 이에 확장현실 제작 기술이 방송 제작에 적극 활용되고 있다. 확장현실은 외부의 제작 환경을 몰입형 LED월 시스템 기반으로 가상의 그래픽으로 내부 스튜디오 제작환경에서 제작 할 수 있어 방송에서는 확장현실 관련 시스템 및 그래픽 콘텐츠, 운영기술 개발이 요구되고 있다. 이에 본 논문은 확장현실 콘텐츠를 제작 할 수 있는 '몰입형 혼합현실 시스템'을 제안한다. 특히, 확장현실 환경에서 원격으로 AR과 VR 콘텐츠의 동기를 맞추어 운영할 수 있도록 개발된 '원격제어 타임라인 디렉터'를 통해 AR/VR 콘텐츠를 재생 및 입출력을 제어할 수 있다. 본 시스템을 통하여 그래픽 기반의 AR/VR 시험 그래픽 콘텐츠 및 실사 기반의 360 콘텐츠 기반 확장현실 시험 콘텐츠를 개발하였으며, 상용카메라 추적시스템과 연개하어 자연스러운 추적 및 합성 그리고 타임라인 디렉터의 사용자 인터페이스를 이용하여 확장현실 콘텐츠를 제작 및 운영 할 수 있음을 확인하였다.