• Korean Journal of Plant Taxonomy
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• v.46 no.4
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• pp.405-412
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• 2016

We investigate the intraspecific morphological variations and geographical distributions from twelve populations (28 samples) of Orobanche coerulescens in Korea. Two main morphological types were detected, i.e., glabrous (G-type) and pilose (P-type), in the external morphology (stem, leaf, bract, calyx, and corolla). The G-type was found on Ulleung-do and Dok-do, and the P-type was observed on the coasts of South Korea, Jeju-do and Ulleung-do. The distinct difference in the morphological types and geographical distribution suggested the existence of two putative lineages with different distribution processes. O. coerulescens, distributed in very limited areas, was only identified as the G-type on Dok-do. Hence, conservation efforts should focus on protecting native habitats to conserve native species and the unique biogeographic significance of this species.

• Journal of Applied Biological Chemistry
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• v.66
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• pp.484-491
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• 2023

Maize is one of the major crops consumed in worldwide, which nutrients accounts for a large amount of starch, but also functional components, and phenolic acid is known to have a high content. Maize is divided into waxy maize, sweet maize, and normal maize with its shape and use, therefore there is also a difference in nutritional composition. This study was conducted to analyze the content of functional components according to the type of maize and to produce natural variation data in consideration of environmental factors. 3 shapes of maize (waxy maize, sweet maize, and normal maize) samples cultivated in 3 regions (Suwon, Daegu, and Hongcheon) were analyzed using HPLC and GC-TOF-MS. Comparing with type through ANOVA, multivariate statistical analysis, Pearson correlation analysis, 28 components, including carotenoids and tocopherols, showed significant differences among a total of 32 components (p <0.05), 15 of them showed very significant differences (p <0.001). When comparing with regions, 15 components showed significant differences and only vanillate, syringate, C23-ol of them showed most significant differences (p <0.001). As a result of principal component analysis, cluster classification was distinguished by shape than by region, with α-carotene, cholesterol for waxy maize, vanillate and stigmasterol for sweet maize, lutein and β-carotene for normal maize had a great effect on cluster formation. It suggests that the content of functional components is more affected by genetic factors than environmental factors.

• Applied Biological Chemistry
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• v.46 no.4
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• pp.305-310
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• 2003

Hydrophobic core variant of ubiquitin appeared to have partially folded structure at pH around 2. The intrinsic tryptophan fluorescence emission maximum of this ubiquitin variant at pH 2 showed slight blue shift compare to that of unfolded state, suggesting that some residual tertiary structures remain in this solvent condition. At the same solvent condition, this ubiquitin variant binds with hydrophobic dye, 8-anilinonaphthalene-1-sulfonic acid(AMS), which is known to bind to exposed hydrophobic surface. Furthermore, far-UV circular dichroic spectrum of this ubiquitin variant in the diminished pH was remarkably different from the far-UV CD spectrum of the native state or unfolded state. Based on the molar ellipticity at 220 nm, this ubiquitin variant at pH 2 appeared to have significant amount of secondary structures. All these observations suggest that this ubiquitin variant in the diminished solvent pH has loosely folded hydrophobic core with some secondary structures, which are key features of molten globule conformation. Since molten globule has long been considered as a protein folding intermediate, it is considered that this hydrophobic core variant ubiquitin will serve as a valuable model to study protein folding process.

• Journal of the Korean Chemical Society
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• v.47 no.5
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• pp.460-465
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• 2003

Structural alteration of p53 and overexpression of p53 protein are the most common genetic abnormalities in various kinds of human cancer. Mutations in the p53 tumor-suppressor gene are usually associated with an advanced development of colorectal cancer characterized by the transition from the adenoma to carcinoma stage. Mutations in exons 5-8 of the p53 gene were analyzed by the polymerase chain reaction-single strand conformation polymorphism(PCR-SSCP) and denaturing high performance liquid chromatography(DHPLC). SSCP analysis detected 7 mutations(C13109>T) in 50 colorectal cancer samples(14%) at exon 5, and DHPLC analysis detected 7 mutations (C13109>T) and 2 mutation(C13202>A, C13204>G) in 50 colorectal cancer samples(18%) at exon 5. All of 9 mutations were proved by sequencing analysis. We conclude that DHPLC is a highly sensitive and specific method for p53 gene mutations.

• The Korean Journal of Mycology
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• v.40 no.2
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• pp.93-97
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• 2012

Gamma ray irradiation mutagenesis was employed to get variants of Pleurotus eryngii with functionally enhanced and improved characteristics. Protoplasts released from P. eryngii were treated with gamma ray radiation under 0.25-1.25 KGy. Protoplast sample that showed fatality rate of 80% at the 0.25 KGy was spreaded on YPMGA (yeast, peptone, malt-extract, glucose, agar) and 500 mycelial colonies were randomly selected from the medium. Of them, 100 mutant strains with mycelial morphology and growth rate that differ to control strain were observed on PDA. The cellulase and laccase activity of 67 gamma ray-irradiated P. eryngii isolates with morphological variation were investigated. Among these, 5 isolates were higher cellulase. In addition, the genetic variation of the mutant strains was analyzed by PCR fingerprinting.

• The Korean Journal of Malacology
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• v.9 no.1
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• pp.27-32
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• 1993

러시아의 극동 연안에서는 꼬막 아과 세 종이 출현하고 있다. 이들 중 Anadara inaequivalvis(Bruguiere, 1789)와 Anadara subcrenata(Lischke, 1869)는 신생대에 전멸된 개체군으로 알려져 있다. Anadara속의 형태학적 변이, 분류 및 지리적 분포에 대한 자료를 제시하였다.

• Investigative Magnetic Resonance Imaging
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• v.17 no.3
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• pp.207-214
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• 2013

Purpose : To evaluate the MRI findings of breast cancer with BRCA mutation. Materials and Methods: We collected information of the breast cancer patients who underwent the test for BRCA gene mutation as well as preoperative breast MRI from January 2007 to December 2010. A total of 185 patients were enrolled; 33 of these patients had BRCA mutations and 152 patients did not. Among them, a total of 231 breast cancers were detected. Images of the 47 breast cancers with BRCA mutation and of the 184 breast cancers without mutations were evaluated to compare the morphologic and enhancement features on MRI. Results: With MR imaging, there were no significant difference in morphologic characteristic between two groups. However, enhancement pattern in the group with BRCA mutation were more likely to have persistent enhancement (p < 0.233), and LN metastasis was more common in breast cancers without BRCA mutation. Breast cancers with BRCA 2 mutation tend to show more persistent enhancement pattern than BRCA 1 mutation. Conclusion: In breast cancer patients with BRCA mutation, MRI didn't show significant difference in morphologic characteristics, however breast cancers with BRCA gene mutation carriers tend to have benign morphologic features on MRI, such as Type 1 kinetic curve enhancement.

• Proceedings of the Plant Resources Society of Korea Conference
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• 2022.09a
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• pp.59-59
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• 2022

Vulpia octoflora (Walter) Rydb.가 경기도 여주시 강천리 일대에서 발견되었다. 본 종은 북아메리카 원산으로 알려져 있으며, 최근 호주와 일본, 우크라이나에서 침입외래식물로 보고된 바 있다. V. octoflora는 주로 건조한 건조한 모래나 자갈 모래밭에 자라는 잡초성 식물로, 도로변을 따라 개방된 지역이나 인위적 교란 지역에서 빈번하게 발견된다. 학자에 따라 북아메리카 지역에 분포하는 개체들의 지리적 분포와 형태 변이를 근거로 종하분류군으로 세분화하기도 하지만, 형태 변이의 일관성 부족으로 인해 종내 변이로 인식하여 통합하여 처리하는 것이 타당하게 여겨진다. 본 종은 최근에 국내에 보고된 침입외래식물인 들묵새아재비와 유사하나, 소수당 달리는 소화의 수가 더 많고, 호영 정단부의 까락의 길이가 짧은 것으로 명확히 구분된다. 좀들묵새에 대한 생태계 위해성 연구는 진행된 바 없으나, 최초 발견지인 여주시 강천섬 일대는 멸종위기 2등급종인 단양쑥부쟁이의 자생지이기 때문에, 새롭게 유입된 외래식물의 잠재적 생태 교란에 따른 위해성 평가 및 확산 방지를 위한 모니터링이 필요할 것으로 여겨진다. 본 연구에서는 주요 형질에 대한 기재와, 지리적 분포, 도해도, 화상자료와 국내에 분포하는 동속 분류군과의 검색표를 제공하고자 한다.

• Korean Journal of Ichthyology
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• v.21 no.2
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• pp.67-75
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• 2009

Morphological and genetic variation of Cottus populations in Korea were compared to each other in the watershed. Morphological variation was analyzed by meristic and morphometric characters, and by fertilized egg sizes. Genetic variation was assessed by amplified fragment length polymorphism (AFLP) fingerprinting. Cottus koreanus populations of rivers and streams running towards the eastern coasts of the Korean Peninsula were significantly different in morphometric characters from populations in rivers and streams running towards the western and southern coasts of the Korean Peninsula, but did not differ in meristric characters and fertilized egg sizes. Cottus populations in the upper streams-Yangyangnamadae Stream, Jusu Stream and Samcheogosip Stream-were the same in meristic and genetic characters. However, the Cottus sp. population from Baebong Stream is related to C. hangiongensis in meristic characteristics and to C. koreanus in the morphometric characteristics of length of the ventral fin ray and in the fertilized egg size. Pairwise genetic distances assessed by the AFLP method among C. koreanus populations were in the range of 0.110 to 0.221. Genetic distances between C. hangiongensis and C. koreanus populations varied from 0.542 to 0.621, and those between the Cottus sp. population of Baebong Stream and C. koreanus populations from 0.222 to 0.304. The result of the UPGMA dendrogram shows the Cottus sp. population of Baebong Stream was clearly separated of other C. koreanus populations.

• Weed & Turfgrass Science
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• v.2 no.2
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• pp.184-190
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• 2013

It is important for genetic resources to collect and identify in native medium-leaf type zoysiagrasses species distributed in Korea. This study was conducted to investigate morphological variation and characteristics of native medium-leaf type zoysiagrasses from coastal, island and inland regions in Korea. Among them, 75 collected lines was confirmed to have various morphological variations, accessions were classified into 2 main based group coastal and inland regions by morphological characteristics and site environment. Group I included Z. sinica type, this group showed 3.7 mm in leaf width, 29 in number of seed per spikelet and 5.0 mm in seed length. Group II included Z. japonica type, this group showed 4.4 mm in leaf width, 42 in number of seed per spikelet and 3.5 mm in seed length. There is a need for additional research on growth characteristics and the molecular level for the introgressive hybridization between species which confirmed that cross-pollination is possible due to protogyny. The individuals showing variations should be preserved as valuable genetic resources for the expansion of variations in zoysiagrasses, and the results of this investigation on the genetic resources collected will be highly valuable in breeding high quality turfgrass.