Browse > Article

Morphological Variations and Genetic Variations Inferred from AFLP (Amplified Fragment Length Polymorphism) Analysis of Cottus Populations (Scorpaeniformes: Cottidae) in Korea  

Byeon, Hwa Kun (Environmental Research Institute, Kangwon National University)
Kim, Keun-Sik (Department of Marine Biotechnology, Soonchunhyang University)
Song, Ha-Yoon (Department of Marine Biotechnology, Soonchunhyang University)
Bang, In-Chul (Department of Marine Biotechnology, Soonchunhyang University)
Publication Information
Korean Journal of Ichthyology / v.21, no.2, 2009 , pp. 67-75 More about this Journal
Abstract
Morphological and genetic variation of Cottus populations in Korea were compared to each other in the watershed. Morphological variation was analyzed by meristic and morphometric characters, and by fertilized egg sizes. Genetic variation was assessed by amplified fragment length polymorphism (AFLP) fingerprinting. Cottus koreanus populations of rivers and streams running towards the eastern coasts of the Korean Peninsula were significantly different in morphometric characters from populations in rivers and streams running towards the western and southern coasts of the Korean Peninsula, but did not differ in meristric characters and fertilized egg sizes. Cottus populations in the upper streams-Yangyangnamadae Stream, Jusu Stream and Samcheogosip Stream-were the same in meristic and genetic characters. However, the Cottus sp. population from Baebong Stream is related to C. hangiongensis in meristic characteristics and to C. koreanus in the morphometric characteristics of length of the ventral fin ray and in the fertilized egg size. Pairwise genetic distances assessed by the AFLP method among C. koreanus populations were in the range of 0.110 to 0.221. Genetic distances between C. hangiongensis and C. koreanus populations varied from 0.542 to 0.621, and those between the Cottus sp. population of Baebong Stream and C. koreanus populations from 0.222 to 0.304. The result of the UPGMA dendrogram shows the Cottus sp. population of Baebong Stream was clearly separated of other C. koreanus populations.
Keywords
Cottus; AFLP; morphology; egg size; genetic variation;
Citations & Related Records
Times Cited By KSCI : 2  (Citation Analysis)
연도 인용수 순위
1 Goto, A. 1981. Life history and distribution of a River sculpin, Cottus hangiongensis. Bull. Fac. Fish Hokkaido Univ., 32: 10-21.
2 Miller, M. 1997. Tools for population genetic analysis (TFPGA) 1.3: a windows program for the analysis of allozyme and molecular population genetic data. Computer software distributed by author. http://www.Marksgenetic software.net/tfpga.htm.
3 변화근. 1995b. 한국산 둑중개속(Cottus)의 분류학적 재검토와 자어의 형태적 특징. 한국어류학회지, 7: 128-134.
4 변화근. 1996. 한국산 둑중개(Cottus poecilopus)의 성적 이형. 한국어류학회지, 8: 14-21.
5 Mariette, S., D. Chagne, C. Lezier, P. Pastuszka, A. Raffin, C. Plomion and A. Lremer. 2001. Genetic distance within and among Pinus pinaster populations: comparison between AFLP and microsatellite marker. Heredity, 86: 469-479.   DOI   ScienceOn
6 전상린. 1998. 한국산 둑중개와 한둑중개의 검색과 분포. 상명대학교 기초과학연구소, 11: 1-14.
7 Bonin, A., D. Ehrich and S. Manel. 2007. Statistical analysis of amplified fragment length polymorphism data: a toolbox for molecular ecologists and evolutionists. Mol. Ecol., 16: 3737-3758.   DOI   ScienceOn
8 Ma, G.C., H.S. Tsao, H.P. Lu and H.T. Yu. 2006. AFLPs congruent with morphological differentiation of Asian common minnow Zacco (Pisces: Cyprinidae) in Taiwan. Zoologica., 35:341-351.   DOI
9 Mori, T. and K. Uchida. 1934. A revised catalogue of the fishes in Chosen. J. Chosen Nat. Hist. Soc., 19: 24.
10 Nei, M. 1975. Molecular population genetics and the theory of evolution (Translated by Wang Jiayu). China Agriculture Press, Beijing, China, pp. 121-123.
11 Seki, S. 1999. AFLP analysis of genetic distance in three populations of Ayu plecoglossus altivelis. Fisheries Science, 6: 888-892.
12 Sung, Y.G., Y.K. Nam, H.S. Han and I.C. Bang, 2007. Genetic diversity and variation of Chinese shrimp Fenneropenaeus chinensis populations as inferred by AFLP fingerprinting. J. Aquaculture, 20: 255-259. (in Korean)
13 Vos, P., R. Hodgers, M. Bleeker, M. Reijans, T. van de Lee, M. Hornes, A. Frijters, J. Pot, J. Peleman, M. Kuiper and M. Zabeau. 1995. AFLP: a new technique for DNA fingerprinting. Nucleic Acids Res., 23: 4407-4414   DOI   ScienceOn
14 Shaklee, J.B. and P. Pauly. 1982. Speciation and evolution of marine fishes studied by electrophoresis analysis of proteins. Pac. Sci., 36: 141-157.
15 Goto, A. 1978. Comparative studies on the maturation process of two types of Cottus nozawae-I. The annual cycle of ovarian development. Japan. J. Ichthyol., 25: 115-123.
16 Knorr, C., H.H. Cheng and J.B. Dodgson. 1999. Application of AFLP markers to genome mapping in poultry. Anim. Genet., 30:28-35.   DOI   ScienceOn
17 Rohlf, S.B. 1992. NTSYS-pc numerical taxonomy and multivariate analysis system, version 1.7. Applied Biostatistics Inc., New York, USA.
18 전상린. 1986. 한국산 둑중개과 및 큰가시고기과 주연성 단수어 의 검색과 분포. 상명여자대학 논문집, 14: 83-115.
19 Goto, A. 1977. Ecological and morphological studies on the freshwater sculpins in Hokkaido. Dissertation, Hokkaido Univ., 207pp.
20 Lee, I.R., Y.A. Lee, H.C. Shin, Y.K. Nam, W.J. Kim and I.C. Bang. 2008a. Genetic diversity of an endangered fish, Iksookimia choii (Cypriniformes), from Korea as assessed by amplified ragment length polymorphism. Kor. J. Limnol., 41: 97-102 (in Korean)
21 Rusell, J.R., J.D. Fuller, M. Macaulay, B.G. Hatz, A. Jahoor, W. Powell and R. Waugh. 1997. Direct comparison of lecels of genetic variation among barley accessions detdcted by RFLPs, AFLPs, SSRs, and RAPDs. Theor. Appl. Genet., 95: 714-722.   DOI   ScienceOn
22 Liu, Z.J., P. Li, B.J. Aegue and R.A. Dunham. 1998. Ingeritance and usefulness of AFLP markers in channel catfish (Ictalurus punctaus), blue catfish (I. furcatus), and their F1, F2, and backcross hybrids. Mol. Gen. Gent., 258: 260-268.   DOI   ScienceOn
23 Goto, A. 1984. Life history and distribution of a River sculpin, Cottus hangensis. J. Japan. Ichthyol., 31: 161-166.
24 Watanabe, M. 1960. Fauna Japonica, Cottidae (Pisces), pp. 11-218.
25 변화근. 1995a. 한국산 둑중개의 생태학적 연구. 강원대학교 대학원 박사학위논문, pp. 92-95.
26 Lee, Y.A., Y.E. Yun, Y.K. Nam and I.C. Bang. 2008c. Genetic diversity of endangered fish Hemibarbus mylodon (Cyprinidae) assessed by AFLP. Kor. J. Aquaculture, 21: 196-200. (in Korean)
27 Lee, W.O., I.R. Lee, H.Y. Song and I.C. Bang. 2008b. Genetic differentiation of the largemouth bass Micropterus salmoides from the major rivers and reserboirs in Korea assessed by AFLP. Kor. J. Limnol., 41: 395-401. (in Korean)
28 Ryouji, F., Y. Choi and Y. Mamoru. 2005. A new species of freshwater sculpin, Cottus koreanus (Pisces: Cottidae) from Korea. Species Distance, 10: 7-17.
29 Goto, A. 1975. Ecological and morphological divergence of the freshwater sculpin, Cottus nozawae Snyder-I. Spawning behavior and process of the devlopment in the post-hatching stage. Bull. Fac. Fish Hokkaido Univ., 26: 31-37.
30 Kim, I.S. and C.H. Youn. 1992. Synopsis of the Family Cottidae (Pisces: Scorpaeniformes) from Korea. Kor. J. Ichthyol., 4:54-79.