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http://dx.doi.org/10.4489/KJM.2012.40.2.93

Mutagenesis of Pleurotus eryngii by Gamma Ray Irradiation  

Kim, Jong-Kun (Graduate School of Bio. & Information Technology, Hankyong National University)
Lim, Seon-Hwa (Graduate School of Bio. & Information Technology, Hankyong National University)
Kim, Il-Joong (Graduate School of Bio. & Information Technology, Hankyong National University)
Lee, Yun-Hae (Mushroom Research Institute, GARES)
Kang, Hee-Wan (Graduate School of Bio. & Information Technology, Hankyong National University)
Publication Information
The Korean Journal of Mycology / v.40, no.2, 2012 , pp. 93-97 More about this Journal
Abstract
Gamma ray irradiation mutagenesis was employed to get variants of Pleurotus eryngii with functionally enhanced and improved characteristics. Protoplasts released from P. eryngii were treated with gamma ray radiation under 0.25-1.25 KGy. Protoplast sample that showed fatality rate of 80% at the 0.25 KGy was spreaded on YPMGA (yeast, peptone, malt-extract, glucose, agar) and 500 mycelial colonies were randomly selected from the medium. Of them, 100 mutant strains with mycelial morphology and growth rate that differ to control strain were observed on PDA. The cellulase and laccase activity of 67 gamma ray-irradiated P. eryngii isolates with morphological variation were investigated. Among these, 5 isolates were higher cellulase. In addition, the genetic variation of the mutant strains was analyzed by PCR fingerprinting.
Keywords
Gamma-ray irradiation; Mutagenesis; Pleurotus eryngii;
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