• Title/Summary/Keyword: 형태학적 검출

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Physical Characteristics and Microsatellite Polymorphisms in Miryang Native Dogs (밀양지방 토종개의 형태학적 특징 및 유전적 다양성 연구)

  • Cho, Byung-Wook;Cho, Gil-Jae
    • Journal of Life Science
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    • v.16 no.4
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    • pp.626-631
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    • 2006
  • This study was carried out to investigate the physical characteristics (height, body lenght, chest depth, head type, ear type, body color, eye type and tail type) and genetic diversity using 15 microsatellite DNA markers (PEZ 1, 5, 8, 10, 11, 12, 13, 15, 16, 17, 20, 21, FHC 2010, FHC 2054 and FHC 2079) in 44 random Miryang native dogs(6 months${\sim}$12 years old). The height, body lenght, and chest depth of Miryang native dogs were 43-55 cm(mean 49.5 cm), 45-60 cm(mean 54.3 cm), and 50-64 cm(mean 57.9 cm), respectively. Miryang native dog was medium sized. The head and eye type were reverse-triangle(100%), triangle (90.9%) and newborn moon(9.1%), respectively. Most of body color had white coat color(93.2%), light pink tongue color(100%), light black anal color(90,9%) and pink claw color(100%). The ear type showed erect ear(100%), and half-curled(56.8%), upward(34.1%), curled(9.1%) in tail type, respectively. Number of alleles observed at a single locus ranged from 2 (PEZ 21 and FHC 2010) to 14 (PEZ 13), with average number of alleles per locus of 6.13. The expected heterozygosities of 15 microsatellite loci were estimated based on gene frequencies. The highest expected heterozygosity, 0.863 was estimated in PEZ 13 locus and the lowest, 0.455 in PEZ 21 and FHC 2010 locus. And the mean expected heterozygosity of 15 microsatellite markers was calculated as 0.635. Polymorphic information content (PIC) values were ranged from 0.348 (PEZ 21 and FHC 2010) to 0.837 (PEZ 13), and the mean PIC value was calculated as 0.570. Of the 15 markers, PEZ 10, PEZ 13, PEZ 17 and FHC 2054 loci have relatively high PIC value (> 0.7) in Miryang native dog. In order to determine the efficieney of parentage control, exclusion probabilities (PE) were calculated for each allele. The highest PE 1 and PE 2 in PEZ 13 locus was caculated to 0.548 and 0.710, respectively. And the total exclusion power in PE 1 and PE 2 was calculated to 0.9895 and 0.9996, respectively. These results can give basic information for perservation and research in Miryang native dog, and phylogenetic relationships of the Korean native dog and Asian dog breeds.

Studies on Anisakiasis Especially Morphological Studies on the Anisakinae Larva (Anisakiasis에 대한 조사연구(調査硏究) 특히 유충(幼蟲)의 형태학적(形態學的) 구조(構造)에 대하여)

  • Rim, Bong-Ho
    • Korean Journal of Veterinary Research
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    • v.21 no.2
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    • pp.105-112
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    • 1981
  • The morphological and structural studios of Anisakinae larva has been carried out since Sept. of 1980. The larva were collected from naturally infested eleven swine of 1,531 examined at Kwang-Ju abattoir and from marine fishes, Somber japonicus, bought at Kwang-Ju fish market. The results observed were as follow : 1. Anisakis larva found in the stomach wall and on the surface of the mucosa were more or less degenerated. According to the progress of degeneration, the cross sections showed varied structures (Fig. 6, 7). 2. Size of the larva both from swine and fishes were measured respectively in average(mm); 18.0 and 18.7 in body length, 0.30 and 0.41 in body width, 1.64 and 1.68 in esophagus(muscular-part), 0.56 and 0.67 in ventriculus (glandular part), and 0.13 and 0.12 in tail. It was notable that body length of the larva in this present data, 18.0mm and 1.87mm, were shorter than those in previous dada, 24.3mm from human cases and 28.4mm from, however, the present data were almost similar to the data, 1.75mm, from swine case. 3. The Boring tooth, Mucron, long ventriculus and short round tail were observed in the larva of this present study. These structures were differentiated from Anisakis type II larvae which was provided with short ventriculus, and conical and tapering tall without mucron. 4. The ventricular appendix and intestnal caecum were not present in the larva. These might be differentiated from other Anisakidae larva such as Terranova larvae, Contracaecum larvae, Raphidascaris larvae and Thynnascaris larvae. 5. The findings through the histological observation were a pair of Y-shaped or butterfly-shaped lateal chords, ventral and dorsal chords, excretory(Renette) cell, high columnar epithelial cells of digestive tract and muscle cells. These morphological characteristics revealed varied features in the structures in the degenerative degree of the larva in the stomach wall. 6. The above-mentioned characteristics of the larva observed could be indentified as Anisakis type I larvae. 7. The reports on natural infestation of domestic animal with Anisakis type I larvae were two swine cases in Korea and Japan respectively, On the other hand two human cases of the larva were reported in Korea and more than one thousand cases in Japan. In Twiwan no reports of human and domestic animal cases could be found.

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A Study on Microbial Aspects of Korean Human Milk by Collection Methods (수집방법에 따른 한국인 모유의 미생물 분포에 관한 연구)

  • 이조윤;배형철;남명수
    • Food Science of Animal Resources
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    • v.23 no.3
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    • pp.269-277
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    • 2003
  • This study was carried out to evaluate the safety of Korean human milk. The microorganisms were identified from human milk of 149 healthy mothers by two collection methods, hand and pump expression. The means of total bacterial counts were 2.33x10$^4$ cfu/mL on the samples collected by the pump expression and 7.83xl0$^3$ cfu/mL on those collected by the hand expression. Therefore, the total bacterial counts of pump expression samples was 9.80xl0$^2$∼3.06x10$^4$ cfu/mL more than that of hand expression samples. The coliform counts of pump expression was 9.36xl0$^3$∼8.57xl0$^4$ cfu/mL more than that of hand expression. However, there was any significant differences of the lactic acid bacterial counts between the two samples collected by each methods. 100 strains of 5 patterns of total bacterial counts were isolated based on the morphology of colony in the standard plate count agar. 13 species were identified among the isolated strains. The dominant species in Korean human milk were Staphylococcus which 7 subspecies identified(81% in the rate of total bacteria, 1.07x10$^4$ cfu/mL). Other species identified were Micrococcus, Bacillus, Providencia, Pseudomonas, Yersinia and Acinetobacter. 36 strains of 6 patterns of lactic acid bacterial counts were isolated based on morphology of colony in the BCP agar. 7 species were identified among the isolated strains. The dominant species of lactic acid bacteria in Korean human milk were Lactobacillus brevis(50.9% in the rate of lactic acid bacteria, 4.72xl0$^4$ cfu/mL). Others species identified(49.1% lactic acid bacteria) were Lactobacillus curvatus, Lactobacillus delbrueckii subsp. lactis, Lactobacillus acidophilus, Leuconostic lactis and Streptococcus salivarius subsp. thermophilus.

Face classification and analysis based on geometrical feature of face (얼굴의 기하학적 특징정보 기반의 얼굴 특징자 분류 및 해석 시스템)

  • Jeong, Kwang-Min;Kim, Jung-Hoon
    • Journal of the Korea Institute of Information and Communication Engineering
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    • v.16 no.7
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    • pp.1495-1504
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    • 2012
  • This paper proposes an algorithm to classify and analyze facial features such as eyebrow, eye, mouth and chin based on the geometric features of the face. As a preprocessing process to classify and analyze the facial features, the algorithm extracts the facial features such as eyebrow, eye, nose, mouth and chin. From the extracted facial features, it detects the shape and form information and the ratio of distance between the features and formulated them to evaluation functions to classify 12 eyebrows types, 3 eyes types, 9 mouth types and 4 chine types. Using these facial features, it analyzes a face. The face analysis algorithm contains the information about pixel distribution and gradient of each feature. In other words, the algorithm analyzes a face by comparing such information about the features.

A human case of gastric anisakiasis by Pseudotewcnova decipiens larva (Pseudoterranova decipiens의 유충에 의한 위 아니사키스증 1례)

  • 손운목;설상영
    • Parasites, Hosts and Diseases
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    • v.32 no.1
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    • pp.53-56
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    • 1994
  • A case of gastric anisaklasis due to the larva of Pseunotewqnova decipiens was confirmed by gastroendoscoplc examination In April 23, 1991. The patient, residing In Pusan, was a 42-year-old housewife, who complained of severe epigastric pawn and recalled that the symptom suddenly attacked her about 6 hours after eating raw Sebqstes inermis. In the gastroendoscopic examination performed about 9 hours after the onset of the symptom, a long whitish nematode larva penetrating the gastric mucosa in the greater curvature of mid-body was found and removed with a biopsy forcep. The nematode was $29.73{\times}0.94mm$ in size, had an intestinal cecum reaching over mfd-level of the ventnculus and was identified as the 4th stage larva of f decfpiens.

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An imported human case of hookworm infection with worms in the rectum (직장에서 성충이 검출된 수입성 구충(鉤蟲) 감염 1례)

  • 용태순;신호준
    • Parasites, Hosts and Diseases
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    • v.30 no.1
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    • pp.59-62
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    • 1992
  • An imported case of rectal hookworm infection was diagnosed by stool examination and recovery of adult worms from the rectal mucosa by sigmoidoscopy. The chief complaints of a patient were diarrhea, abdominal pain and weight loss for about 1 month after returning from his travel abroad to the Southeast Asia. Leukocytosis ($16, 750/{\mu}\ell$) and peripheral eosinophilia (33.7%) were noticed without anemia. Typical hookworm eggs were detected by stool examination, and 3 worms were collected by sigmoidoscopy from rectal mucosa of this patient. Those worms were confirmed as adult worms of Ancylostoma duodenale(♂:1, ♀: 2) based on their morphological characteristics. The symptoms were relieved after treatment with anthelmintics. This case was considered as one of the imported parasitic infections in Korea, and a rare case of hookworm infection on human rectal mucosa.

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Recognition of Finger Language Using FCM Algorithm (FCM 알고리즘을 이용한 지화 인식)

  • Kim, Kwang-Baek;Woo, Young-Woon
    • Journal of the Korea Institute of Information and Communication Engineering
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    • v.12 no.6
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    • pp.1101-1106
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    • 2008
  • People who have hearing difficulties suffer from satisfactory mutual interaction with normal people because there are little chances of communicating each other. It is caused by rare communication of people who have hearing difficulties with normal people because majority of normal people can not understand sing language that is represented by gestures and is used by people who have hearing difficulties as a principal way of communication. In this paper, we propose a recognition method of finger language using FCM algorithm in order to be possible of communication of people who have hearing difficulties with normal people. In the proposed method, skin regions are extracted from images acquired by a camera using YCbCr and HSI color spaces and then locations of two hands are traced by applying 4-directional edge tracking algorithm on the extracted skin lesions. Final hand regions are extracted from the traced hand regions by noise removal using morphological information. The extracted final hand regions are classified and recognized by FCM algorithm. In the experiment using images of finger language acquired by a camera, we verified that the proposed method have the effect of extracting two hand regions and recognizing finger language.

Evaluation of Microbiological, Physicochemical, and Sensory Characteristics of Korean Slice Beef Jerky (한국형 슬라이스 우육포의 미생물학적, 이화학적 및 관능적 품질특성 평가)

  • Kim, Hyoun-Wook;Lee, Eun-Kyung;Han, Doo-Jeong;Choi, Ji-Hun;Kim, Cheon-Jei;Paik, Hyun-Dong
    • Food Science of Animal Resources
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    • v.27 no.1
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    • pp.42-46
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    • 2007
  • We evaluated the microbial safety and quality characteristics of Korean slice beef jerky, and investigated these properties over 28-day and 90-day storage periods at room temperature ($25^{\circ}C$) and elevated temperature ($35^{\circ}C$). After microbial counts of all samples, mesophilic bacteria were detected at 1.23 Log CFU/g at day 0. Counts of mesophilic bacteria did not change significantly in all samples, and coliforms and Bacillus cereus were not detected in all samples during storage at either $25^{\circ}C$ or $35^{\circ}C$. TBA values, Aw, and pH were investigated. The Aw of korean slice beef jerky stored at room temperature was 0.71 at day 0, and was reduced to 0.61 after 90 days. The TBA value increased as storage time increased, and its TBA value was 0.48 after 90 days of storage. The pH of all samples did not change significantly. At $35^{\circ}C$ storage, TBA values, Aw, pH were not significantly different than those stored at $25^{\circ}C$. Also, the sensory properties of all samples were not significantly different between two storage temperatures. In conclusion, these results suggest Koran slice beef jerky ould be used as basic study for development of the commercial beef jerky.

Studies on the Purification and Biochemical Properties of Vitellin in the Antheraea yamamai Guerin-Meneville II. Biochemical Properties of Vitellin (천잠(Antheraea yamamai) Vitellin의 분리와 생화학적 특성에 관한 연구 II. Vitellin의 생화학적 특성)

  • 김철명;문재유
    • Journal of Sericultural and Entomological Science
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    • v.31 no.2
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    • pp.82-90
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    • 1989
  • Antheraea yamamai vitellin was purified from matured eggs by polyacrylamide gel electrophoresis for characterization of its biochemical properties : molecular weight, sugar and lipid composition, amino acid composition and electron microscopic morphology, etc. 1. A yamamai vitellin was composed of two subunits, large and small, showing different mobility in SDS-polyacrylamide gel electrophoresis. 2. The molecular weight of the vitellin was estimated to be approximately 450,000 dalton and the large and small subunits were 174,000 dalton and 44,000 dalton, respectively. 3. The vitellin seemed to be a glycolipoprotein since it showed a positive reaction to coomassie brilliant blue, sudan black B and PAS staining. Both subunits were similiar in this aspect. 4. Lipid of the witellin reveraled several different types including saturated lipids. 5. When the vitellin was incubated at 7$0^{\circ}C$ for 60 minites its apoprotein still cross-reacted to the specific antiserum to the native vitellin. Its sugar components were also detected by PAS staining, but its lipid portion was not detected by sudan black B staining. 6. Its amino acid composition was similar to that of other insects, but its glycine content was peculiarly very high. 7. The vitellin molecule was spherical in shape with a diameter of 14$\pm$0.8nm by negatively.

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Changes of Physicochemical Properties of Soaked Glutinous Rice During Preparation of Yu-Kwa (유과제조시 찹쌀의 침지중 이화학적 성분변화에 관한 연구)

  • Lim, Young-Hee;Lee, Hyun-Yu;Jang, Myung-Sook
    • Korean Journal of Food Science and Technology
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    • v.25 no.3
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    • pp.247-251
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    • 1993
  • Physico-chemical characteristics of glutinuous rice during soaking treatment at different temperature for Yu-Kwa (Korean traditional glutinous rice cake) processing were determined. Crude fat and protein contents of soaked glutinous rice decreased up to 30% to 50%, respectively, compared to raw rice, but there was a little difference in soaking periods. As the soaking period increased pH of soaking solution was decreased from 6.4 to 3.3, while Brix was increased from 0.5 to 13.6. Titration acidity of soaking solution and soaked rice were increased to 22.9 and 3.4, respectively, as the soaking period increased to 9 days at $20^{\circ}C$ soaking, but it was increased rapidly to 29.8 and 4.0, respectively, at $30^{\circ}C$. The three kinds of free sugar such as glucose, sucrose and maltose were detected. Glucose was the major produced free sugar during soaking, sucrose was not detected after 1 day soaking period. Characteristics of amylographic viscosity on soaked rice showed that there are no difference in pasting temperature by the soaking time and temperature, but the maximum viscosity was drastically increased on $1{\sim}2\;day$ soaking period and decreased rapidly after $2{\sim}3\;day$.

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