• Title/Summary/Keyword: 형태학적 검출

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A Calibration Method for Multimodal dual Camera Environment (멀티모달 다중 카메라의 영상 보정방법)

  • Lim, Su-Chang;Kim, Do-Yeon
    • Journal of the Korea Institute of Information and Communication Engineering
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    • v.19 no.9
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    • pp.2138-2144
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    • 2015
  • Multimodal dual camera system has a stereo-like configuration equipped with an infrared thermal and optical camera. This paper presents stereo calibration methods on multimodal dual camera system using a target board that can be recognized by both thermal and optical camera. While a typical stereo calibration method usually performed with extracted intrinsic and extrinsic camera parameter, consecutive image processing steps were applied in this paper as follows. Firstly, the corner points were detected from the two images, and then the pixel error rate, the size difference, the rotation degree between the two images were calculated by using the pixel coordinates of detected corner points. Secondly, calibration was performed with the calculated values via affine transform. Lastly, result image was reconstructed with mapping regions on calibrated image.

Isolation and Characterization of Bacillus subtilis CH-10 Secreting Cellulase from Cattle Manure (우분으로부터 Bacillus subtilis CH-10의 분리 및 균주가 분비하는 Cellulase의 특성에 관한 연구)

  • Kim, Tae-Il;Han, Jung-Dae;Jeon, Byoung-Soo;Ha, Sang-Woo;Yang, Chang-Bum;Kim, Min-Kyun
    • Korean Journal of Microbiology
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    • v.35 no.4
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    • pp.277-282
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    • 1999
  • A bacterium producing the extracellular cellulase was isolated from cattle feces and screened as cellulase activity was excellent upon congo red straining method and activity measurements. Isolate was identified as Bacillus subtilis CH-10 on the basis of morphological and biochemical properties as well as cellular fatty acids composition. The enzyme which the isolate secretes had the optimum initial pH and temperature for its induction was 7.5 and 50${\circ}C$, respectively. The maximum CMCase activity in crude enzyme solution was observed at pH 7.5 and 75${\circ}C$ and was stable for pH 7.5 to 9.0 to maintain 70% activity. When the isolate was cultured in CMC media at 37${\circ}C$ for 24 hrs, CMCase and FPase activity was 1.13 U/㎖and 0.16U/㎖, respectively whereas Avicelase and ${\beta}$-glucosidase activity was not detected. When crude supernatant was used for zymogram, three major bands, cel 1, cel 2 and cel 3, were detected approximately 39, 41 and 57 KDa, respectively on CMC-SDS-PAGE.

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Survey of Anisakis spp. infection in wild populations of marine fish caught from coastal areas of Korea (한국 연안에서 채집된 자연산 해산어의 아니사키스 유충 감염)

  • Choi, Hee-Jung;Jun, Eun-Ji;Lee, Deok-Chan;Cho, Mi-Young;Jee, Bo-Young;Im, Young-Su;Park, Myoung-Ae;Seo, Jung-Soo
    • Journal of fish pathology
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    • v.22 no.3
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    • pp.201-210
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    • 2009
  • Although Anisakis type larvae have been shown to cause various injuries directly or indirectly in humans and animals, the epidemiological studies on these larval infestations are in insufficient state. The status of larval infestation was investigated in 989 fishes of 44 species, which are inhabiting around the east-westernsouth costal area of Korea during the period from March 2007 to February 2008. The Anisakis type larvae were infected approximately 38% (377 fishes) in 989 fishes. Most of the worms were identified as Anisakis simplex type I by morphological finding and 18S ribosomal DNA sequence analysis. In the seasonal variations of infestation, most of the fishes showed higher infestation rate during spring and summer, while the fishes such as herring Clupea pallasii did during winter. From the histopathological studies of infested fishes, it has been observed that Anisakis type larvae are harbouring mainly around the intestinal viscera such as liver, pancreas, stomach, pylolic cecum, and cloaca.

Application of MALDI-TOF mass spectrometry-based identification of foodborne pathogen tests to the Korea Food Standard Codex (MALDI-TOF 질량분석기를 이용한 식품중독균 확인시험 적용)

  • Ha, Miyoung;Son, Eun Jung;Choi, Eun Jeong
    • Korean Journal of Food Science and Technology
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    • v.48 no.5
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    • pp.437-444
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    • 2016
  • Rapid and reliable identification of microorganisms is important to maintain food quality and to control safety. MALDI-TOF MS-based identification methods are relatively fast and simple compared to other conventional methods including gram staining and biochemical characterization. A colony on subcultured media can be directly prepared on the analysis plate without further complex treatments. In this study, we confirmed the applicability of MALDI-TOF MS-based identification of foodborne pathogens such as Salmonella Enteritidis/Typhimurium, Staphylococcus aureus, Vibrio parahaemolyticus, Clostridium perfringens, Listeria monocytogenes, Yersinia enterocolitica, Bacillus cereus, Campylobacter jejuni, Campylobacter coli, and Cronobacter sakazakii on the Korea Food Standard Codex. MALDI-TOF MS data of the pathogenic reference strains were incorporated into a commercial MicroID (ASTA Inc.) database. Other pathogenic reference strains and seven isolates from various food samples were correctly identified to the species level by using the MicroID database. In conclusion, MALDI-TOF MS is comparable with commercial biochemical identification.

Understanding of Intrauterine Environment Changes based on Proteomics and Bioinformatics during Estrous Cycle (단백체학과 생물정보학을 이용한 자궁 내 환경의 이해)

  • Lee, Sang-Hee;Lee, Seunghyung
    • Journal of Life Science
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    • v.29 no.5
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    • pp.621-630
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    • 2019
  • Fertilization is the beginning of a new life that occurs in the female uterine. The female reproductive tract is composed ovary, oviduct, uterine, vagina and cervix, their physiological features are regulated by estrous cycle. Of these, uterine is a main point to establish embryo development and implantation, and intercommunication between embryo and uterine environment is necessary for suitable pregnancy. Endometrium is part of the uterine, its morphology is repetitively changed by hormones, and characteristic of uterine fluid from endometrium is also changed. Recently, massive proteins of endometrium and uterine fluid can be detected according to develop proteomics and bioinformatics and have been accelerated the understanding of the reproductive biology fields. Moreover, the massive protein information is actively studying with deeply studied theory such as sex hormone signal pathway and angiogenesis in mammals. In this paper, we review understanding of endometrium remodeling, uterine gland and fluid during estrous cycle, additionally studies on endometrium and uterine fluid based on proteomics techniques. Lastly, we introduced methods of the protein-protein correlation using bioinformatics tool that interaction with hormone receptors, representative angiogenetic factors and detected proteins using proteomics in endometrium and uterine fluid. This review will be useful to understanding the study on search of new cell mechanism in endometrium and uterine fluid.

Quality of Insambob Containing Added Raw and Red Ginseng Extract (수삼과 홍삼액을 첨가하여 취반한 인삼밥의 품질학적 특성)

  • Lee, Ka-Soon;Kim, Gwan-Hou;Kim, Hyun-Ho;Seong, Bong-Jae;Kim, Sun-Ick;Han, Seung-Ho;Lee, Gyu-Hee
    • Journal of the Korean Society of Food Science and Nutrition
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    • v.41 no.8
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    • pp.1151-1157
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    • 2012
  • This study was conducted to investigate methods of increasing raw ginseng consumption. To accomplish this, Insambob was prepared by adding minced raw ginseng (MRG), ground raw ginseng (GRG) or extracts of red ginseng (RGE). Sensory quality, textural properties, and changes in the ginsenoside and free amino acid composition of the Insambob then were investigated. Insambob containg 50% RGE had the best color, flavor and texture, but that containing 10% GRG had the best taste and overall acceptability. The hardness and adhesiveness were highest for containing 10% GRG and decreased as the amount of ginseng added increased. However, the hardness increased, while the adhesiveness of Insambob containg RGE decreased significantly as the amount added increased. Moreover, the ginsenoside composition changed upon addition of ginseng, with the levels of ginsenoside-Rb1, -Rb2, -Rb3, -Rc, -Re, -Rd, -Rg1, and -Rf decreasing and ginsenoside-Rh2, -Rh1, and -Rg3 newly appearing. Finally, the total free amino acid contents of Insambob increased upon addition of MRG, GRG and RGE.

A comparative study of the morphology of the ovipositors of wood-boring insects, Tremex fuscicornis and Leucospis japonica (목질을 천공하는 얼룩송곳벌(Tremex fuscicornis)과 밑드리좀벌(Leucospis japonica) 산란관의 형태적 특징 비교)

  • Kim, Ji Yeong;Park, Ji-Hyun;Kwon, Oh Chang;Kim, Jinhee
    • Korean Journal of Environmental Biology
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    • v.38 no.4
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    • pp.554-562
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    • 2020
  • Tremex fuscicornis (Siricidae), known as the xylophagous horntail, and Leucospis japonica (Leucospidae), known as the parasitoid wasp, are wood-boring wasps belonging to the order Hymenoptera. These insects are interesting sources of biological inspiration for the development of drilling mechanisms. To study the biomimicry aspects, the morphological characters of the ovipositor of T. fuscicornis and L. japonica were analyzed using a stereoscopic microscope, a field emission scanning electron microscope, and an optical microscope. There were many differences in the ovipositors between the two species, such as shape, length, surface structure, and arrangement of the teeth. Evenly arranged teeth were developed at the tip of both the dorsal valve and the ventral valve of the ovipositor of T. fuscicornis and looked like a rotating drill bit. In contrast, in L. japonica, the teeth, which looked like a saw, were found only on the ventral valve. Moreover, the tip of the ovipositor of T. fuscicornis was symmetrically divided into four parts, while that of L. japonica was divided into three parts having a 2:1:1 ratio. However, in the case of T. fuscicornis, after the 14th tooth, four parts melded into three parts maintaining a 2:1:1 ratio, and a dovetail joint was found on the horizontal cross-section of the ovipositor that allowed vertical movement for making a hole. These morphological differences of the ovipositor may be due to the insects' lifestyles and phylogenetic distance. Finally, zinc was commonly found at the tip of the ovipositors of both species, a probable result of ecological adaptation created by drilling wood.

Depth Extraction of Convergent-Looking Stereo Images Based on the Human Visual System (인간시각체계에 기초한 교차시각 스테레오 영상의 깊이 추출)

  • 이적식
    • The Journal of Korean Institute of Communications and Information Sciences
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    • v.27 no.4A
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    • pp.371-382
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    • 2002
  • A camera model with optical axes parallel has been widely used for stereo vision applications. A pair of input ages are obtained from a convergent-looking stereo camera model based on the human visual system in this per, and each image is divided into quadrant regions with respect to the fixation point. The reasoning of quadrant partitions is based on the human visual system and is proven by a geometrical method. Image patches : constructed from the right and left stereo images. A modified cepstrum filter is applied to the patches and disparity vectors are determined by peak detection algorithm. The three-dimensional information for synthetic ages is obtained from the measured disparity and the convergent stereo camera model. It is shown that the experimental results of the proposed method for various stereo images are accurate around the fixation point like the human visual system.

A study on local facial features using LDP (LDP를 이용한 지역적 얼굴 특징 표현 방법에 관한 연구)

  • Cho, Young Tak;Jung, Woong Kyung;Ahn, Yong Hak;Chae, Ok Sam
    • Convergence Security Journal
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    • v.14 no.5
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    • pp.49-56
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    • 2014
  • In this paper, we proposed a method for representing local facial features based on LDP (Local Directional Pattern). To represent both PFF (Permanent Facial Features) and TFF (Transient Facial Features) effectively, the proposed method configure local facial feature vectors based on overlapped blocks for each facial feature in the forms of various size and shape. There are three advantages - it take advantages of geometric feature based method; it shows robustness about detection error using movement characteristics of each facial feature; and it shows reduced sampling error because maintain spatial information caused by block size variability. Proposed method shows better classification accuracy and reduced amount of calculation than existing methods.

Epithelioma of farmed catfish Silurus asotus in Korea (국내산 메기, Silurus asotus의 상피종)

  • Yu, Jin-Ha;Park, Sung-Woo;Kim, Dong-Wan
    • Journal of fish pathology
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    • v.22 no.2
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    • pp.107-113
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    • 2009
  • Catfish Silurus asotus sampled from a pond farm in Korea, was affected with an epithelial tumor resembling catfish Ameiurus nrbulosus lip and mouth epithelioma. The neoplasm appeared in the form of solitary, multiple, large and fleshy masses upon the skin, fin or barbel. The epithelial tumor proliferation causes papillary folds supported by connective tissue cords. These folds extended above the normal skin surface and into the underlying dermis. In some area, proliferation of mucous cells or club cells were observed. The epithelial cells of the tumor were dispersed with no orderly arrangement. This is the first report on epithelioma of catfish in Korea.