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http://dx.doi.org/10.9721/KJFST.2016.48.5.437

Application of MALDI-TOF mass spectrometry-based identification of foodborne pathogen tests to the Korea Food Standard Codex  

Ha, Miyoung (Nonghyup Food Research Institute)
Son, Eun Jung (Nonghyup Food Research Institute)
Choi, Eun Jeong (Nonghyup Food Research Institute)
Publication Information
Korean Journal of Food Science and Technology / v.48, no.5, 2016 , pp. 437-444 More about this Journal
Abstract
Rapid and reliable identification of microorganisms is important to maintain food quality and to control safety. MALDI-TOF MS-based identification methods are relatively fast and simple compared to other conventional methods including gram staining and biochemical characterization. A colony on subcultured media can be directly prepared on the analysis plate without further complex treatments. In this study, we confirmed the applicability of MALDI-TOF MS-based identification of foodborne pathogens such as Salmonella Enteritidis/Typhimurium, Staphylococcus aureus, Vibrio parahaemolyticus, Clostridium perfringens, Listeria monocytogenes, Yersinia enterocolitica, Bacillus cereus, Campylobacter jejuni, Campylobacter coli, and Cronobacter sakazakii on the Korea Food Standard Codex. MALDI-TOF MS data of the pathogenic reference strains were incorporated into a commercial MicroID (ASTA Inc.) database. Other pathogenic reference strains and seven isolates from various food samples were correctly identified to the species level by using the MicroID database. In conclusion, MALDI-TOF MS is comparable with commercial biochemical identification.
Keywords
MALDI-TOF MS; Foodborne pathogens; Microbiological tests;
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1 Bremer H, Dennis PP. Modulation of chemical composition and other parameters of the cell by growth rate. pp. 167-182. In: Escherichia coli and Salmonella: Cellular and Molecular Biology. Neidhardt FC (ed). ASM Press, Washington, DC, USA (1996)
2 Ryzhov V, Fenselau C. Characterization of the protein subset desorbed by MALDI from whole bacterial cells. Anal. Chem. 73:746-750 (2001)   DOI
3 Ash C, Farrow John AE, Dorsch M, Stackebrandt E, Collins MD. Comparative analysis of Bacillus anthracis, Bacillus cereus, and related species on the basis of reverse transcriptase gene sequencing of 16S rRNA. Int. J. Syst. Bacteriol. 41: 343-346 (1991)   DOI
4 Bessede E, Solecki O, Sifre E, Labadi L, Megraud F. Identification of Campylobacter species and related organisms by matrix assisted laser desorption ionization-time of flight (MALDI-TOF) mass spectrometry. Clin. Microbiol. Infect. 17: 1735-1739 (2011)   DOI
5 Kim HW, Ham JS, Seol KH, Han SH, Park BY, Oh MH. MALDI-TOF MS system for the identification of microorganisms in milk and dairy products. J. Milk Sci. Biotechnol. 30: 131-137 (2012)
6 Wieser A, Schneider L, Jung J, Schubert S. MALDI-TOF MS in microbiological diagnostics-identification of microorganisms and beyond (mini review). Appl. Microbiol. Biotechnol. 93: 965-974 (2012)   DOI
7 Murray PR. What is new in clinical microbiology-microbial identification by MALDI-TOF Mass Spectrometry. J. Mol. Diagn. 14: 419-423 (2012)   DOI
8 Dingle TC, Butler Wu SM. MALDI-TOF mass spectrometry for microorganism identification. Clin. Lab. Med. 33: 589-609 (2013)   DOI
9 Bourassa L, Butler-Wu SM. MALDI-TOF Mass Spectrometry for Microorganism Identification. Vol. 42. pp. 37-85. In: Methods in Microbiology. Norris JR, Ribbons DW (eds). Academic Press, Cambridge, MA, USA (2015)
10 Anhalt JP, Fenselau C. Identification of bacteria using mass spectrometry. Anal. Chem. 47: 219-225 (1975)   DOI
11 Weisburg WG, Bams SM, Pelletier DA, Lane DJ. 16S Ribosomal DNA amplification for phylogenetic study. J. Bacteriol. 173: 697- 703 (1991)   DOI
12 Claydon MA, Davey SN, Edwards-Jones V, Gordon DB. The rapid identification of intact microorganisms using mass spectrometry. Nat. Biotechnol. 14: 1584-1586 (1996)   DOI
13 Holland RD, Wilkes JG, Ralli F, Sutherland JB, Persons CC, Voorhees KJ, Lay Jr. JO. Rapid identification of intact whole bacteria based on spectral patterns using matrix-assisted laser desorption/ ionization with time-of-flight mass spectrometry. Rapid Commun. Mass Sp. 10: 1227-1232 (1996)   DOI
14 Krishnamurthy T, Ross PL, Rajamani U. Detection of pathogenic and non-pathogenic bacteria by matrix-assisted laser desorption ionization time-of-flight mass spectrometry. Rapid Commun. Mass Sp. 10: 883-888 (1996)   DOI
15 Hansen BM, Leser TD, Hendriksen NB. Polymerase chain reaction assay for the detection of Bacillus cereus group cells. FEMS Microbiol. Lett. 202: 209-213 (2001)   DOI
16 Chen ML, Tsen HY. Discrimination of Bacillus cereus and Bacillus thuringiensis with 16S rRNA and gyrB gene based PCR primers and sequencing of their annealing sites. J. Appl. Microbiol. 92: 912-919 (2002)   DOI
17 Bakke I, Schryver PD, Boon N, Vadstein O. PCR-based community structure studies of Bacteria associated with eukaryotic organisms: A simple PCR strategy to avoid co-amplification of eukaryotic DNA. J. Microbiol. Meth. 84: 349-351 (2011)   DOI
18 Smole SC, King LA, Leopold PE, Arbeit RD. Sample preparation of gram-positive bacteria for identification by matrix assisted laser desorption/ionization time-of-flight. J. Microbiol. Meth. 48: 107-115 (2002)   DOI
19 Alatoom AA, Cunningham SA, Ihde SM, Manrekar J, Patel R. Comparison of ditrect colony method versus extraction method for identfication gram-positive cocci by use of bruker biotyper matrix-assisted laser desorption/ionization time-of-flight mass spectrometry. J. Clin. Microbiol. 49: 2868-2873 (2011)   DOI
20 Bizzini A, Durussel C, Bille J, Greub G, Prodhom G. Performance of matrix-assisted laser desorption/ionization time-of-flight mass spectrometry for identification of bacterial strains routinely isolated in a clinical microbiology laboratory. J. Clin. Microbiol. 48: 1549-1554 (2010)   DOI
21 Haigh J, Degun A, Eydmann M, Millar M, Wilks M. Improved performance of bacterium and yeast identification by commercial in the clinical microbiology laboratory. J. Clin. Microbiol. 49: 3441 (2011)   DOI
22 Evason DJ, Claydon MA, Gordon DB. Effects of ion mode and matrix additives in the identification of bacteria by intact cell mass spectrometry. Rapid Commun. Mass Sp. 14: 669-672 (2000)   DOI
23 Fenselau C, Demire PA. Characterization of intact microorganisms by MALDI mass spectrometry. Mass Spectrom. Rev. 20: 157-171 (2001)   DOI
24 Ford BA, Burnham CD. Optimization of routine identification of clinically relevant gram-negative bacteria by use of matrixassisted laser desorption ionization time-of-flight mass spectrometry and the Bruker Biotyper. J. Clin. Microbiol. 51: 1412-1420 (2013)   DOI
25 TeKippe EM, Shuey S, Winkler DW, Butler MA, Burnham CD. Optimizing identification of clinically relevant gram-positive organisms by use of the Bruker Biotyper matrix-assisted laser desorption ionization time-of-flight mass spectrometry system, J. Clin. Microbiol. 51: 1421-1427 (2013)   DOI
26 Seng P, Drancourt M, Gouriet F. Ongoing revolution in bacteriology: Routine identification of bacteria by matrix-assisted laser desorption ionization time-of-flight mass spectrometry. Clin. Infect. Dis. 49: 543-551 (2009)   DOI
27 Drobniewski FA. Bacillus cereus and related species. Clin. Microbiol. Rev. 6: 324-338 (1993)   DOI
28 Mellmann A, Bimet F, Bizet C. High interlaboratory reproducibility of matrix-assisted laser desorption ionization-time of flight mass spectrometry-based species identification of nonfermenting bacteria. J. Clin. Microbiol. 47: 3732-3734 (2009)   DOI
29 Croxatto A, Prod'hom G, Greub G. Application of MALDI-TOF mass spectrometry in clinical diagnostic microbiology. FEMS Microbiol. Rev. 36: 380-407 (2012)   DOI