• Korean Journal of Veterinary Research
• /
• v.41 no.4
• /
• pp.571-578
• /
• 2001

To evaluate if the apoptotic fragment assay could be used to estimate the dose prediction after radiation exposure, we examined apoptotic mouse crypt cells per 1,000 cells after whole body $^{60}Co$ $\gamma$-rays and 50MeV ($p{\rightarrow}Be^+$) cyclotron fast neutron irradiation in the range of 0.25 to 1 Gy, respectively. The incidence of apoptotic cell death rose steeply at very low doses up to 1 Gy, and radiation at all doses tigger rapid changes in crypt cells in stem cell region. These data suggest that apoptosis may play an important role in homeostasis of damaged radiosensitive target organ by removing damaged cells. The curve of dose-effect relationship for the data of apoptotic fragments was obtained by the linear-quadratic model $y=0.18+(9.728{\pm}0.887)D+(-4.727{\pm}1.033)D^2$ ($r^2=0.984$) after $\gamma$-rays irradiation, while $y=0.18+(5.125{\pm}0.601)D+(-2.652{\pm}0.7000)D^2$ ($r^2=0.970$) after neutrons in mice. The dose-response curves were linear-quadratic, and a significant dose-response relationship was found between the frequency of apoptotic cell and dose. These data show a trend towards increase of the numbers of apoptotic crypt cells with increasing dose. Both the time course and the radiation dose-response curve for high and low linear energy transfer (LET) radiation modalities were similar. The relative biological effectiveness (RBE) value for crypt cells was 2.072. In addition, there were significant peaks on apoptosis induction at 4 and 6h after irradiation, and the morpholoigcal findings of the irradiated groups were typical apoptotic fragments in crypt cells that were hardly observed in the control group. Thus, apoptosis in crypt cells could be a useful in vivo model for studying radio-protective drug sensitivity or screening test, microdosimetric indicator and radiation-induced target organ injury. Since the apoptotic fragment assay is simple, rapid and reproducible in the range of 0.25 to 1 Gy, it will also be a good tool for evaluating the dose response of radiation-induced organ damage in vivo and provide a potentially valuable biodosimetry for the early dose prediction after accidental exposure.

• Radiation Oncology Journal
• /
• v.17 no.3
• /
• pp.238-248
• /
• 1999

Purpose : To assess the histomorphologic changes in the rat lung injury induced by radiation, to determine whether captopril reduces the rat lung injury and to evaluate change in TNF-${\alpha}$ and TGF-${\beta}$ in rat lung damage by radiation and captopril Methods and material : Right lungs in male Sprague-Dawley rats were divided irradiation alone (10, 20, 30 Gy) or radiation (same dose with radiation alone group) with captopril (500 mg/L). Radiation alone group were sacrificed at twelve hours and eleven weeks after radiation and radiation with captopril group (captopril group) were sacrificed at eleven weeks after radiation with captopril. We examined the light microscope and electron microscopic features in the groups. Results : In radiation alone group, there were patch parenchymal collapse and consolidation at twelve hours after radiation. The increase of radiation dose shows more prominent the severity and broader the affected areas. Eleven weeks after radiation, the severity and areas of fibrosis had increased in proportion to radiation dose given in the radiation alone group. There was notable decrease of lung fibrosis in captopril group than in radiation alone group. The number of mast cells rapidly increased with increase of radiation dose in radiation alone group and the degree of increase of mast cell number and severity of collagen accumulation more decreased in captopril group than in radiation alone group. In radiation alone group, expression of TNF-${\alpha}$ and TGF-${\beta}$ increased according to increase of radiation dose at twelve hours after radiation in both group. At eleven weeks after radiation, expression of TGF-${\beta}$ increased according to increase of radiation dose in radiation group but somewhat decreased in captopril group. In the captopril group the collagen deposition increased but less dense than those of radiation alone group. The severity of perivascular thickening, capillary change, the number and degranulation of mast cells more decreased in the captopril group than in the radiation alone group. Conclusion : It is concluded that the effect of captopril in the rat lungs after radiation was considered to be due to its effect on inhibition of mast cells and reduction of collagen deposition, and captopril may be protect in lung damage after radiation. We observed expression of TNF-${\alpha}$ and TGF-${\beta}$ increased at the early phase after radiation and expression of TGF-${\beta}$ increased in proportion to increase of radiation dose at the chronic phase after radiation. This results will contribute to future investigation in reduction mechanism of captopril in lung damage after radiation.

• Journal of the Computational Structural Engineering Institute of Korea
• /
• v.17 no.1
• /
• pp.39-47
• /
• 2004

In this paper, the defects of two-dimensional anisotropic elastic bodies are identified by using the boundary element method. The use of numerical models that contain only boundary integral terns reduces the dimensionality of the problem by one. This advantage is particularly important in problems such as crack mechanics. Avoiding domain meshing is also particularly advantageous in the solution of inverse problems since it overcomes mesh perturbations and simplifies the procedure. In this paper, nondestructive approaches for the existing isotropic materials are extended to analyze the elastic bodies made of anisotropic materials such as composites. After verifying that the proposing boundary element model is in good agreement with numerical results reported by other investigators, the effect of noise in the measurements on the identifiability is studied with respect to different design parameters of layered composites. Sample studies are carried out for various layup configurations and loading conditions. The effects of the layup sequences in detecting flaw of composites is explored in this paper.

• Journal of the Korea Academia-Industrial cooperation Society
• /
• v.21 no.7
• /
• pp.590-596
• /
• 2020

The fabric used for military winter inner clothing(top) is quilted with padded cotton to provide warmth. This quilting fabric is generally manufactured with yarns that intersect and are sewn substantially between the fabric and cotton. Thus, it is impossible to separate the fabric and cotton once after the quilting fabric is manufactured, which can result in a significant loss of fabric and cotton when separated. In this study, after fabricating the quilting fabric, we investigated a method to stabilize change rate of thickness and increase the warmth keeping property through subsequent processing without damaging the fabric. A relatively method of passing the quilting fabric through a part of the cotton production facility was used generally, and the following results were obtained. This indicates that after the quilting fabric was manufactured, the warmth keeping property was improved through the subsequent processing steps, so that the change rate of thickness due to washing was stabilized.

• Journal of the Microelectronics and Packaging Society
• /
• v.21 no.1
• /
• pp.25-29
• /
• 2014

In this study, an optical PCB was proposed which can overcome the limitations of the conventional PCB, and a new structure with pluggable lens was considered for a high-efficient passive alignment. The structure was a lens-added optical waveguide for the improvement of misalignment between the lens and the waveguide in the alignment. Also, as it had a barrier-type structure to prevent the surface damage of the lens by desorption, the high-efficient passive alignment can be realized. The structure was designed by optimizing the simulation and the fabrication process of the pluggable lens structure was conducted using the repetitive photolithography and the thermal reflow. The optical waveguide with the lens-integrated pluggable interconnection was fabricated by the imprint process using the polydimethylsiloxane(PDMS) replica mold. Therefore, we confirmed the possibility of pluggable lens-added optical waveguide structure fabrication for high-efficient passive alignment.

• Journal of Korean Association for Spatial Structures
• /
• v.12 no.3
• /
• pp.47-54
• /
• 2012

The roof surface of spatial structures is often damaged or destroyed because of its light weight roof structure and materials. Many of large scale stadiums have roof structure framed with steel truss or stay cable and wrapped or covered with membrane material Teflon, and this membrane material is easily damaged and its loss is quite serious. Through such examples, it was found that the studies on wind proof design of roofs of large space structures were not sufficiently made. This study conducted wind pressure experiment and fluid analysis in order to examine the aerodynamic characteristic of the roof shape of hyperbolic paraboloid spatial structures. Although the biggest minimum peak wind pressure coefficient was shown in the edges of the roof in the wind origin direction, it decreases with the advancement to the longitudinal direction of the roof.

• Journal of Navigation and Port Research
• /
• v.28 no.1
• /
• pp.43-49
• /
• 2004

Defects inspection of commodities are very important with those design and manufacturing process and essential to strengthen the competitiveness of those. If on-line automatic defects detection is performed without damaging to products, the production cost shall be curtailed through the reducing man-power, economical management of Q.C(Quality Control). In this paper, it is suggested three spatial filtering methods which can extract the necessary information in case of defects being on the surface of object like iron plate. In addition, the dependence of filtering characteristics on parameters such as the pitch and width of slits is analyzed and the surface defect detection system is constructed. Several experiments were carried out for determining the adequate spatial filtering method through comparing and analyzing effects of parameters like defect's size and shape, intensity of light, noise of coherent source and slit number.

• Radiation Oncology Journal
• /
• v.28 no.3
• /
• pp.141-146
• /
• 2010

Purpose: We examined the radioprotective effects of 5-androstendiol (5-AED), a natural hormone produced in the reticularis of the adrenal cortex, as a result of intestinal damage in gamma-irradiated C3H/HeN mice. Materials and Methods: Thirty mice (C3H/HeN) were divided into three groups; 1) non-irradiated control group, 2) irradiated group, and 3) 5-AED-treated group prior to irradiation. Next, 5-AED (50 mg/kg per body weight) was subcutaneously injected 24 hours before irradiation. The mice were whole-body irradiated with 10 Gy for the histological examination of jejunal crypt survival and the determination of the villus morphology including crypt depth, crypt size, number of villi, villus height, and length of basal lamina, as well as 5 Gy for the detection of apoptosis. Results: The 5-AED pre-treated group significantly increased the survival of the jejunal crypt, compared to irradiation controls (p<0.05 vs. irradiation controls at 3.5 days after 10 Gy). The evaluation of morphological changes revealed that the administration of 5-AED reduced the radiation-induced intestinal damages such as villus shortening and increased length of the basal lamina of enterocytes (p<0.05 vs irradiation controls on 3.5 day after 10 Gy, respectively). The administration of 5-AED decreased the radiation-induced apoptosis in the intestinal crypt, with no significant difference between the vehicle and 5-AED at 12 hours after 5 Gy. Conclusion: The results of this study suggest that the administration of 5-AED has a protective effect on intestinal damage induced by $\gamma$-irradiation. In turn, these results suggest that 5-AED could be a useful candidate for radioprotection against intestinal mucosal injury following irradiation.

• Asian Journal of Turfgrass Science
• /
• v.25 no.1
• /
• pp.17-21
• /
• 2011

Korean bennudagrass collections showed diverse genetic variations in their morphology, growth habit, and cytological aspects. Chromosome number and nuclear DNA content of the bennudagrasses indicated a ploidy level ranging from triploid (2n=3x) to hexaploid (2n=6x). In this study, we investigated the different responses of antioxidant enzymes (superoxide dismutase, catalase, peroxidase, ascorbate peroxidase) and cell membrane stability of those bennudagrass cytotypes to lower temperature and shorter day length, which meets a dormant induction in Korea. All the antioxidant enzymes were found to be higher during dormant stage, while the heme-containing catalase which converts hydrogen peroxide ($H_2O_2$) to water and oxygen molecules was activated before dormant initiation in the three cytotypes except for hexaploid bennudagrass. The triploid and tetraploid which exhibited relatively finer leaves and a rapid establishment speed were found to show increased activities of superoxide dismutase and peroxidase enzyme. The malondialdehyde(MDA) which is a product of lipid peroxidation in the cell membrane damaged by the hydroxyl radical was increased in all cytotypes as temperature declined, and tri- and tetraploids which had more protective antioxidant enzymes demonstrated a significantly lower MDA production. Similarly electrolyte leakage was higher in penta- and hexaploidy, seemingly more damage to cell membrane when low temperature was implemented. Results indicated that antioxidant responses of different cytotypes were genetically specific, which needs to be investigated the relevance with the low temperature tolerance in the bermudagrass further at the molecular level.

• Korean Journal of Plant Resources
• /
• v.33 no.4
• /
• pp.287-292
• /
• 2020

Lathyrus palustris often used as a treatment for inflammation of the kidneys in Korean traditional medication. Generally, drugs for arthritis have anti-inflammatory and antinociceptive properties. However, the validity of the anti-inflammatory effect has not been scientifically investigated so far. Therefore, the purpose of the research was to investigate the latent anti-inflammatory ability of L. palustris using the ethanol extract. To evaluate the anti-inflammatory activities, we examined the inflammatory arbitrators such as a nitric oxide (NO) and prostaglandin E₂ (PGE₂) on RAW 264.7 cells. Our results indicated that ethanol extract significantly inhibited the lipopolysaccharide E (LPS) derived PGE₂ production in RAW 264.7 cell. The inhibitory activity of ethanol extract for PGE₂ tests with inhibition ratio showed in 40 ㎍/mL. Overall, PGE₂ tests had a higher inhibitory effect on inflammation than NO tests. This result anticipated that the ethanol extract from L. palustris is a good candidate for developing the origin of anti-inflammatory agents.