Browse > Article

Antioxidant Enzyme Activity and Cell Membrane Stability of Korean Bermudagrass Genotypes Different in Ploidy at Dormant Stage  

Lee, Geung-Joo (Dept. of Horticulture, Mokpo National Univ.)
Lee, Hye-Jung (Dept. of Crop Science, Chungbuk National University)
Ma, Ki-Yoon (Dept. of Horticulture, Mokpo National Univ.)
Jeon, Young-Ju (Dept. of Horticulture, Mokpo National Univ.)
Kim, In-Kyung (Dept. of Horticulture, Mokpo National Univ.)
Publication Information
Asian Journal of Turfgrass Science / v.25, no.1, 2011 , pp. 17-21 More about this Journal
Abstract
Korean bennudagrass collections showed diverse genetic variations in their morphology, growth habit, and cytological aspects. Chromosome number and nuclear DNA content of the bennudagrasses indicated a ploidy level ranging from triploid (2n=3x) to hexaploid (2n=6x). In this study, we investigated the different responses of antioxidant enzymes (superoxide dismutase, catalase, peroxidase, ascorbate peroxidase) and cell membrane stability of those bennudagrass cytotypes to lower temperature and shorter day length, which meets a dormant induction in Korea. All the antioxidant enzymes were found to be higher during dormant stage, while the heme-containing catalase which converts hydrogen peroxide ($H_2O_2$) to water and oxygen molecules was activated before dormant initiation in the three cytotypes except for hexaploid bennudagrass. The triploid and tetraploid which exhibited relatively finer leaves and a rapid establishment speed were found to show increased activities of superoxide dismutase and peroxidase enzyme. The malondialdehyde(MDA) which is a product of lipid peroxidation in the cell membrane damaged by the hydroxyl radical was increased in all cytotypes as temperature declined, and tri- and tetraploids which had more protective antioxidant enzymes demonstrated a significantly lower MDA production. Similarly electrolyte leakage was higher in penta- and hexaploidy, seemingly more damage to cell membrane when low temperature was implemented. Results indicated that antioxidant responses of different cytotypes were genetically specific, which needs to be investigated the relevance with the low temperature tolerance in the bermudagrass further at the molecular level.
Keywords
Bermudagrass cytotype; Electrolyte leakage; Low temperature stress; Malondialdehyde; Reactive oxygen species;
Citations & Related Records
Times Cited By KSCI : 2  (Citation Analysis)
연도 인용수 순위
1 Heng-Moss, T., G. Sarath, F. Baxendale, D. Novak, S. Bose, X. Ni, and S. Quisenberry. 2004. Characterization of oxidative enzyme changes in buffalograsses challenged by Blissus Oxxiduus. J. Econ. Entomol. 97:1086-1095.   DOI   ScienceOn
2 Kang, S.Y., G.J. Lee, K.B. Lim, H.J. Lee, I.S. Park, S.J. Chlmg, J.B. Kim, D.S. Kim, and H.K. Rhee. 2008. Genetic diversity among Korean bermudagrass(Cynodon spp.) ecotypes characterized by morphological, cytological and molecular approaches. Mol. Cells 25: 163-171.
3 Lee, G.J., R. N. Carrow, and R.R. Duncan. 2005. Growth and water relation responses to salinity stress in halophytic seashore paspalum ecotypes. Sci. Hortic. 104:221-236.   DOI   ScienceOn
4 Lee, H.J. G.J. Lee, D.S. Kim, J.B. Kim, J.H. Ju, and S.Y. Kang. 2008. Selection and physiological characterization of glyphosate-tolerant zoysiagrass mutants derived from a gamma ray irradiation. Kor. J. Hort. Sci. Technol. 26:454-463.
5 Mittler, R. and B.A. Zilinskas. 1993. Detection of ascorbate peroxidase activity in native gels by inhibition of ascorbate dependent reduction of nitroblue tetrazolium. Anal. Biochem. 112:540-546.
6 Moller, I.M., P.E. Jensen, and A. Hansson. 2007. Oxidative modifications to cellular components in plants. Annu. Rev. Plant Biol. 58:459-481.   DOI   ScienceOn
7 Ptter, J. 1974. Peroxidase. p. 685-690. In: Bergmeyer, H.U.(ed.). Methods of enzymatic analysis, vol. 2, Academic Press, N.Y.
8 Trenholm, L.E., M.J. Schlossberg, G. Lee, and W. Parks. 2000. An evaluation of multi-spectral responses on selected turfgrass species. Int. J. Remote Sensing 21:709-721.   DOI   ScienceOn
9 Aebi, H. 1974. Catalase. p. 673-684. In: H.D. Bergmeyer (ed.). Methods of enzymatic analysis, vol. 2, Academic Press, N.Y.
10 Beyer, W.F. and I. Fridovich. 1987. Assaying for superoxide dismutase activity: some large consequences of mionor changes in conditions. Anal. Biochem. 161 :559-566.   DOI   ScienceOn
11 Cyril, J., G.L. Powell, R.R. Duncan, and W.V. Baird. 2002. Changes in membrane polar lipid fatty acids of seashore paspalum in response to low temperature exposure. Crop Sci. 42:2031-2037.   DOI
12 Dhindsa, R.S. and W. Matowe. 1981. Drought tolerance in 21 two mosses correlated with enzymatic defense against lipid peroxidation. J. Exp. Bot. 32:79-91.   DOI