• Korean Journal of Ichthyology
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• v.32 no.4
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• pp.239-244
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• 2020

The morphological characteristics of a larval fish (7.8 mm in body length) collected off Chuuk, Micronesia were highly similar to those of larval Omosudis sp., except fin development and body length. It was identified as Omosudis lowii by partial mitochondrial 12S rRNA gene analysis. The morphological traits of the larval fish validated by the molecular genetic marker will be informative for species-level identification of larval Omosudis lowii.

• Korean Journal of Clinical Laboratory Science
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• v.48 no.1
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• pp.15-21
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• 2016

Aspergillus is the most common opportunistic fungus causing infection. Aspergillus is the most morphologically identified in the laboratory. Recently, molecular genetic methods have been proposed for identification of fungi that unidentified morphologically or identified genus level. Of 475 cases of Aspergillus isolated from clinical specimens, there were Aspergillus fumigatus 257 (54.1%), A. niger 101 (21.3%), A. flavus 43 (9.1%), A. terreus 29 (6.1%), Aspergillus nidulans 2 (0.4%), Aspergillus clavatus 1 (0.2%), and the Aspergillus species 42 (8.8%). Eleven cases of unidentified or identified at the genus level included Aspergillus fumigatus 5, Aspergillus falvus 1, Aspergillus terreus 1, and Aspergillus lentulus 1 was identified in the sequencing of the strain level. It was identified as Aspergillus versicolor 2, and Emericella parvathecia 1. 92.2% of Aspergillus was identified as a possible morphological, 8.8% could not be identified at the species level. Sequence-based molecular analysis using the ITS and D1D2 is considered useful for identification of the species level.

• Korean Journal of Ichthyology
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• v.31 no.4
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• pp.249-254
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• 2019

One specimen of Albula argentea (296.3 mm in standard length), belonging to the family Albulidae, was collected in a set net in the coastal waters of Jejudo Island, Korea. As the adult A. argentea has not been caught from Korean waters, we described its morphological characters based on the collected specimen and compared them with those of the previous reports on A. argentea and A. koreaus. As a result, the present specimen was characterized by having 68~73 pored lateral line scales (vs. 77~78 for A. koreana), 72~73 vertebrae pored lateral line scales (vs. 77~80) and none streak on the check (vs. yellowish streak). Molecular analysis using the mitochondrial cytochrome b DNA sequences was also conducted to confirm the correctness of species identification and taxonomic status of the sample.

• Korean Journal of Microbiology
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• v.25 no.3
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• pp.238-243
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• 1987

In this study, an acidoduric Streptomyces strain was isolated and identified from acidic forest soil around Dankook University, Cheonan Campus. This isolated strain had rod-shaped, smooth, non-motile spore and the shape of spore chain was compact spiral. This structure appeared similar to the sporangium of the genus Streptosporangium but this strain proved to be a Streptomyces strain by an electron microscopic study and cell wall analysis. This strain showed a best growth on neutral medium, was also able to grow on the acidic media of pH 4.0 and pH 5.0. The color determination of this strain on various agar media and other physiological tests were carried out by ISP-methods. From these results, the isolated strain was considered to be Streptomyces mirabilis.

• Proceedings of the Korean Society for Applied Microbiology Conference
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• 1986.12a
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• pp.518.1-518
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• 1986

광합성 세균의 수소생성 기작에 대한 연구의 일환으로 광합성 세균에서 생성되는 색소의 성분을 조사하였다. 분리한 광합성 세균 K-7은 수소생성능이 뛰어난 균주로 조사된 홍색 비유황세균으로서 생리, 형태 및 배양학적 조사에 의하여 Rhodopseudomonas spheroides로 분류하였다. Type culture인 Rhodopseudomonas spheroides NCIB 8253과 비교 연구하였으며, SEM(Scanning Electron Microscope)하에서 기존균주와 분리균주의 형태학적인 특징을 확인하였다. 군 동정의 주요열쇠가 되는 색소성분을 조사한 결과, 균주 K-7에서 추출된 carotenoids로는 spheroidene의 산화형태인 OH-spheroidenone이 주성분이었고, Neurosporene, Lycopene, Anhydro- rhodovibrin, Rhodovibrin등이 동정되었다.

• Proceedings of the Korean Society for Applied Microbiology Conference
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• 1978.04a
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• pp.96.1-96
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• 1978

미생물학적 감산현상에 의하여 과실주중의 산미를 조절하기 위한 방법으로 효모에 의하여 사과산을 알코올로 분해하는 Maloalcohol 발효를 유도하고자 하여 본 연구를 하였다. 이미 발표된 Schizosaccharomyces pombe 0-77보다 단 시간에 강력히 사과산을 소비하는 효모균을 딸기의 과피로부터 분리, 동정하였다. 본 분리균을 J. Lodder의 “The Yeasts”에 준하여 동정한 바 분열법에 의하여 증식하며 포자를 형성하고 galactose 를 발효못하므로 Schizosacoha-romyces 속으로 분류하였다. 위균사를 잘 형성하며 melibiose를 발효하므로 Schizosaccharomyces japonicus와 일치 하였다. 그러나 변종의 동정은 어려우나 위균사의 형태로 보아 Schizosaccharomyces japonicus var. japonicus로 동정하였다.

• Korean Journal of Ichthyology
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• v.34 no.1
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• pp.1-7
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• 2022

A larva of the deep-sea angler fish, Himantolophus groenlandicus (2.2 mm BL), identified based on the complete mitochondrial DNA sequence, was collected at the surface of the western North Pacific. The postflexion stage larva had a round body, small teeth, incipient dorsal fin rays, eyes slightly recessed in the lower part, and melanophores on the gills and parietal and dorsal regions. These morphological features differ from a description of a larva reported as the same species with similar size (2.1 mm BL). The genetic and morphological information of our specimen should be useful for identifying larval H. groenlandicus.

• Journal of Sericultural and Entomological Science
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• v.15 no.2
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• pp.69.1-69
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• 1973

1973년도 춘기 및 추기에 발생된 병잠에서 체취한 병원체가 새로운 형태의 것으로 우리나라에서는 그 동정이 불가능하여 영국에 그 동정을 의뢰하는 한편 이에 대한 병원성을 검토하여 그 결과를 발표코자한다.

• Proceedings of the Korean Society for Applied Microbiology Conference
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• 1978.10a
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• pp.205.4-206
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• 1978

벼 문길병균 Pellicularia sasakii에 대하여 강한 항균력을 나타내는 항생물질 생산균의 배양상의 특성과 형태적, 생리적 특성을 종합적으로 조사하여 항생물질 생산균을 동정하였다. 동정 결과 본 생산균은 Streptomyces griseorubi-ginosus로 동정되었고 배양상의 조건에 근소한 차이를 나타내므로 상기균주의 새로운 변종으로서 Streptomyces griseorubiginosus var. kyungiensis 명명하였다.

• Korean Journal of Clinical Laboratory Science
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• v.52 no.3
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• pp.229-236
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• 2020

Sixty-five molds isolated from clinical specimens were included in this study. All the isolates were molds that could be identified morphologically, strains that are difficult to identify because of morphological similarities, and strains that require species-level identification. PCR and direct sequencing were performed to target the internal transcribed spacer (ITS) region, the D1/D2 region, and the β-tubulin gene. Comparative sequence analysis using the GenBank database was performed using the basic local alignment search tool (BLAST) algorithm. The fungi identified morphologically to the genus level were 67%. Sequencing analysis was performed on 62 genera and species level of the 65 strains. Discrepancies were 14 (21.5%) of the 65 strains between the results of phenotypic and molecular identification. B. dermatitidis, T. marneffei, and G. argillacea were identified for the first time in Korea using the DNA sequencing method. Morphological identification is a very useful method in terms of the reporting time and costs in cases of frequently isolated and rapid growth, such as Aspergillus. When molecular methods are employed, the cost and clinical significance should be considered. On the other hand, the molecular identification of molds can provide fast and accurate results.