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http://dx.doi.org/10.15324/kjcls.2020.52.3.229

Molecular Analysis of Pathogenic Molds Isolated from Clinical Specimen  

Lee, Jang Ho (Department of Clinical Laboratory Science, Semyung University)
Kwon, Kye Chul (Department of Laboratory Medicine, College of Medicine, Chungnam National University)
Koo, Sun Hoe (Department of Laboratory Medicine, College of Medicine, Chungnam National University)
Publication Information
Korean Journal of Clinical Laboratory Science / v.52, no.3, 2020 , pp. 229-236 More about this Journal
Abstract
Sixty-five molds isolated from clinical specimens were included in this study. All the isolates were molds that could be identified morphologically, strains that are difficult to identify because of morphological similarities, and strains that require species-level identification. PCR and direct sequencing were performed to target the internal transcribed spacer (ITS) region, the D1/D2 region, and the β-tubulin gene. Comparative sequence analysis using the GenBank database was performed using the basic local alignment search tool (BLAST) algorithm. The fungi identified morphologically to the genus level were 67%. Sequencing analysis was performed on 62 genera and species level of the 65 strains. Discrepancies were 14 (21.5%) of the 65 strains between the results of phenotypic and molecular identification. B. dermatitidis, T. marneffei, and G. argillacea were identified for the first time in Korea using the DNA sequencing method. Morphological identification is a very useful method in terms of the reporting time and costs in cases of frequently isolated and rapid growth, such as Aspergillus. When molecular methods are employed, the cost and clinical significance should be considered. On the other hand, the molecular identification of molds can provide fast and accurate results.
Keywords
Mold; Molecular identification; Morphological identification; Sequencing;
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