• Journal of Yeungnam Medical Science
• /
• v.29 no.1
• /
• pp.42-44
• /
• 2012

Plasma cell myelomas generally manifest as bone or soft-tissue tumors with variable mass effects, pain, and infiltrative behavior. Extramedullary involvement occurs most commonly in the spleen, liver, lymph nodes, and kidneys, but intracranial involvement in plasma cell myeloma is a rare extramedullary manifestation. These authors recently encountered a case of intracranial involvement of plasma cell myeloma. A 69-year-old man was hospitalized for headache and mental changes. Brain CT showed subdural hemorrhage caused by plasma cell myeloma. Plasma cell myeloma with intracranial involvement has poor prognosis, and the patient in this case died from acute complications, such as subdural hemorrhage. Based on this case report, it is suggested that more effective treatment regimens of plasma cell myeloma with intracranial involvement be developed. Moreover, a screening method and decision on the appropriate time for intracranial involvement are needed for plasma cell myeloma patients.

• The Journal of Korean Society of Virology
• /
• v.29 no.2
• /
• pp.137-144
• /
• 1999

Transformation efficiency, virus multiplication and foreign gene expression were characterized in the insect cells transformed with Autographa californica nuclear polyhedrosis virus (AcNPV) immediate early 1 gene (IE1). Transformation efficiency of insect cells by AcNPV IE1 gene vector horboring foreign gene was approximately 8-fold higher in the Sf9 cells transformed previously with AcNPV IE1 gene than in the normal Sf9 cells. Virus multiplication and foreign gene expression of recombinant baculovirus in the Sf9 cells transformed with AcNPV IE1 gene were similar to those of the normal Sf9 cells. These results suggest that transformed cells displaying foreign gene product by using AcNPV IE1 gene promoter will be useful for the diverse applications of insect cells.

• Journal of Plant Biotechnology
• /
• v.30 no.1
• /
• pp.65-71
• /
• 2003

This research aims the production of anti-tumor substances through in vitro culture of hairy roots transformed by Agrobacterium rhizogenes in Artemisia sylvatica MAX and the effect of culture conditions on optimum growth of hairy roots. We investigated the optimum medium, pH, carbon source, sucrose, light, Fe and polyamine conditions of various lines of hairy roots (NK3, NK4, YX. NK3-10) induced from Artemisia sylvatica to increase the optimum growth of hairy roots. MS medium was the best for optimum growth of hairy root clone, NK3-S10. The optimum culture period was 4 weeks for NK3-S10. The optimum sucrose concentration was 3.5%. The optimum concentration of FeSO$_4$, spermine and spermidine was 0.1 mM, 10 mM and 100 mM, respectively.

• Journal of The Korean Society of Grassland and Forage Science
• /
• v.13 no.3
• /
• pp.170-176
• /
• 1993

The yield, viability and continuous culture of isolated Italian ryegrass protoplasts were investigated. The effects of cold treatment (4^{\circ}C.$) for 7 days and basic LS medium supplemented with 5mg/l $AgNO_3$ showed effectively on embryogenic callus induction and regeneration responses of immature and mature embryos or young inflorescences subcultured every 4 weeks on basic medium. The optimum combinations of growth regulator on the regeneration responses was 0.2mg/l BAP and 2mg/l 2, 4-D. Calli induced inflorescences were suspended in its liquid medium for 5 days before enzyme treatment. Maximum protoplast yield and viability were obtained after digestion in enzyme solution contained 4% cellulase R10. 2% macerozyme and 2% pectinase in 0.6M mannitol. Cell division and microcalli development were observed in isolated protoplasts cultured in agarose culture of KM8P medium.

• Journal of the Korean Society of Laryngology, Phoniatrics and Logopedics
• /
• v.22 no.1
• /
• pp.56-59
• /
• 2011

Inflammatory pseudotumor is characterized by the presence of a mass that may mimic malignancy and is composed of spindle cells mixed with variable amounts of extracellular collagen, lymphocytes, and plasma cells It is benign tumor commonly involves lung and orbit and rarely that occurs in the head and neck The cause and pathogenesis of inflammatory pseudotumor still remain controversial. Some bacterial infection or chronic irritation may have prompted some speculation that an initially reactive process can change into an overt neoplastic disease. It tends to affect children and young adults than old ages. We present a case of inflammatory pseudotumor involving the tongue base, in a 73-year-old patient with repeated oral bleeding that aggravated over a few days. Clinical examination showed angiomatous polyp like findings in oropharynx that easy bleeding tendency. CT images showed well defined soft tissue mass with focal enhancement. After surgery, tumor was finally diagnosed inflammatory pseudotumor by histopathologic examinations showed plasma cells and inflammatory cells in variable fibrotic tissues.

• Journal of Veterinary Clinics
• /
• v.21 no.4
• /
• pp.398-401
• /
• 2004

A ten-year-old, female Yorkshire terrier dog with intermittent vomiting, weight loss, polydipsia and depression was referred to the Veterinary Medical Teaching Hospital of Konkuk University. On the radiological survey, gas and fluid-filled gastric distention was detected. No contrast medium entraneed to the small bowel, in 60 minutes after contrast medium administration. And marked fluid accumulation in gastric body and thickening of the pyloric mucosa were found with ultrasonograph. So gastric obstruction by reason of pyloric stenosis was strongly suspected. At the surgery, hypertrophied mucosa was resected adequately, and Heineke-Mikulicz pyloroplasty was applied. Microscopically the symptom was characterized by gastric mucosa hyperplasia and mild diffuse Iymphoplasmocytic gastritis. So this condition was diagnosed as chronic hypertrophic pyloric gastropathy. Intermittent vomiting and mild depression were shown for 9 days after the operation. After that, the patient was recovered gradually. One month later, the patient had normal appetite and activity without complications.

• Korean journal of applied entomology
• /
• v.41 no.4
• /
• pp.279-284
• /
• 2002

Flacherie symptom was found in the fifth instar larvae of silkworm, Bombyx mori. The bacterial pathogen was isolated from the hemolymph of the infected silkworm and identified. The isolated bacteria caused a significant flacherie pathogenicity to the fifth instar larvae of B. mori when $5{\times}10^{6}$ cfu (colony-forming unit) of the bacteria was injected into each larva. The infected larvae began to die at 6 days after injection and resulted in complete mortality at 10 days. The bacterium was identified as Staphylococcus gallinarum based on the morphological and physiological characteristics described in Bergey's manual. This identification was further supported by the characters of carbohydrate utility analyzed from a bacterial identification system ($MicroLog^{\circledR}$) and also by the molecular structure of 165-23S rDNA internal transcribed spaces. As an insecticidal action, S. gallinarum caused hemolymph septicemia by its cytotoxic effect on the hemocytes of B. mori.

• Korean Journal of Plant Tissue Culture
• /
• v.26 no.2
• /
• pp.77-83
• /
• 1999

We have investigated the effect of culture conditions on tropane alkaloids (scopolamine, hyoscyamine) production in hairy root cultures of Hyoscyamus niger L. induced by Agrobacterium tumefaciens $A_4$T. SH medium was the best for tropane alkaloids production from the hairy root clones, HN18 and HN57. The optimum culture peroid was 5 weeks for HN18 clone and 6 weeks for HN57 clone, respectively. The optimum sucrose and dextrose concentrations in tropane alkaloids productivity were 3% and 2%, respectively. The growth of both HN18 and HN57 clones increased with as sucrose concentration increase up to 7% sucrose, but tropane alkaloid contents was significantly decreased. In the HN18 clone, the optimum concentration of sucrose for alkaloids productivity was 5% and those of dextrose was 2%. The productivity of tropane alkaloids for HN57 clone under dextrose treatments was quite a low level compared to sucrose treatments.

• Journal of Plant Biotechnology
• /
• v.33 no.3
• /
• pp.161-169
• /
• 2006

Plant metabolomics is a plant biology field for identifying all of the metabolites found in a certain plant cell, tissue, organ, or whole plant in a given time and conditions and for studying changes in metabolic profiling as time goes or conditions change. Metabolomics is one of the most recently developed omics for holistic approach to biology and is a kind of systems biology. For holistic approach, metabolomics frequently uses chemometrics or multivariate statistical analysis of metabolic profillings. In plant biology, metabolomics is useful to determine functions of genes often in combination with DHA microarrays by analyzing tagged mutants of the model plants Arabidopsis and rice. This review paper attempted to introduce basic concepts of metabolomics and practical uses of multivariate statistical analysis of metabolic profiling obtained by $^1$H HMR and Fourier transform infrared spectrometry.

• Korean Journal of Plant Taxonomy
• /
• v.35 no.4
• /
• pp.313-335
• /
• 2005

Many plants take up soluble silica from the soil, and they deposit in plant tissues. Silica bodies are most commonly found in the leaf epidermis. Silica body is important factor in growth and development of plant. It help to maintain rigidity in stem and linear leaves, and its content may be correlated with resistance to fungal disease. In this study, several morphologically distinctive forms of silica bodies on Cyperaceae in Korea are recognized, which are eventually classified into two groups. Silica body type of first group (Rhynchospora, Fuirena, Scleria, Carex, Eriophorum) is A-type, which is conical in shape. Silica body type of second group (Cyperus, Scirpus, Eleocharis, Fimbristylis, Kyllinga, Bulbostylis, Lipocarpha) is B-type, which is conical in shape with numerous satellite body. These types are consistent enough to use as characters in taxonomic studies within genus. Silica body type may be associated with habitat: the species in dry region have A-type, whereas the species in wet region have B-type. These results suggested that shapes and presence or absence of silica body might be valuable to systematic analysis.