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Identification of a Pathogenic Bacterium, Staphylococcus gallinarum, to Bombyx mori  

김길호 (상주대학교 농학부 잠사곤충학과)
박영진 (안동대학교 자연과학대학 농생물학과)
김용균 (안동대학교 자연과학대학 농생물학과)
Publication Information
Korean journal of applied entomology / v.41, no.4, 2002 , pp. 279-284 More about this Journal
Abstract
Flacherie symptom was found in the fifth instar larvae of silkworm, Bombyx mori. The bacterial pathogen was isolated from the hemolymph of the infected silkworm and identified. The isolated bacteria caused a significant flacherie pathogenicity to the fifth instar larvae of B. mori when $5{\times}10^{6}$ cfu (colony-forming unit) of the bacteria was injected into each larva. The infected larvae began to die at 6 days after injection and resulted in complete mortality at 10 days. The bacterium was identified as Staphylococcus gallinarum based on the morphological and physiological characteristics described in Bergey's manual. This identification was further supported by the characters of carbohydrate utility analyzed from a bacterial identification system ($MicroLog^{\circledR}$) and also by the molecular structure of 165-23S rDNA internal transcribed spaces. As an insecticidal action, S. gallinarum caused hemolymph septicemia by its cytotoxic effect on the hemocytes of B. mori.
Keywords
Bombyx mori; Flacherie; Septicemia; Entomopathogenic bacteria; Staphylococcus gallinarum;
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