• Title/Summary/Keyword: 현탁배양

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Effect of Indole on the Cell Growth and Synthesis of Indirubin in Suspension Culture of Polygonum tinctorium LOUR (쪽 현탁배양(懸濁培養)에서 Indole이 세포 생장과 Indirubin 생성에 미치는 영향)

  • Chung, Eun-Suk;Chae, Young-Am
    • Korean Journal of Medicinal Crop Science
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    • v.3 no.2
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    • pp.96-99
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    • 1995
  • This experiment was carried out to analyze the effect of indole on the synthesis of indirubin in suspension culture of Polygonum tinctorium. Adding indole and L-tryptophan into culture media was re­vealed that indirubin was synthesized in callus grown on solid medium containing indole and proper concentration of indole for indirubin production was decided as 200mg/1. Indirubin content in suspension culture was higher than in solid medium with considerable amount of indirubin secresed into media in suspension culture and highest quantity of indirubin was obtained when indole was added into medium after 20 days suspension culture.

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Plant Regeneration from Embryogenic Suspension Culture of Orchardgrass (Dactylis glomerata L.) (오차드그래스의 현탁배양으로부터 부정배 형성과 식물체 재분화)

  • 이효신;권용삼;이병현;원성혜;김기용;조진기
    • Journal of The Korean Society of Grassland and Forage Science
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    • v.20 no.1
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    • pp.7-12
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    • 2000
  • This study was carried out to improve the ability of embryo formation and the efficiency of plant regeneration from suspension cultured cells of seed derived calli of orchardgrass (Dactylis glomerata L.). The frequency of formation of round cell and cell colonies was highest at 50 days after suspension culture in $N_6$ medium supplemented with $4\;g/{\ell}$ casein hydrolysate (CH), $20\;g/{\ell}$ sucrose and $30\;g/{\ell}$ sorbitol. The highest frequency of plant regeneration and somatic embryo formation was obtained from suspension cultured cells of 60 days. Addition of CH ($4\;g/{\ell}$) in suspension culture medium gave the highest frequency of embryo formation (39.6%) and plant regeneration (73.0%).

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Orchardgrass의 종자유래 캘러스로부터 부정배형성과 식물체 재분화

  • 이효신;이병현;원성혜;김기용;김미혜;정동민;조진기
    • Proceedings of the Korean Society of Grassland Science Conference
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    • 1999.06a
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    • pp.73.2-74
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    • 1999
  • Orchardgrass의 종자배양 유래의 캘러스를 현탁배양하여 현탁배양기간별 부정배형성정도와 식물체 재분화율 등에 대한 몇 가지 실험을 수행한 바, 2주 간격으로 4회계 대배양 하였을 때 계대배양 횟수가 증가됨에 따라 식물체 재분화율이 증가되었다. 종자배양에서 형성된 캘러스의 현탁배양에서 모양이 둥근세포와 그들의 세포괴는 배양 30일 후에 최대치를 나타내었고, 그 이후는 감소하였다.(중략)

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Protoplast Culture and Plant Regeneration of Rice (벼의 원형질체 배양과 식물체 재분화)

  • 이성호;이수인;김주현;코킹 에드워드 씨
    • KOREAN JOURNAL OF CROP SCIENCE
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    • v.42 no.3
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    • pp.306-316
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    • 1997
  • Embryogenic calli were induced from mature seed scutella of anther culture-derived rice variety Zhonghua 8. Cell suspension cultures were initiated from friable embryogenic calli and utilized as source material for protoplast isolation. Generally, the older and finer cell suspensions gave higher protoplast yields than younger suspension cultures. Protoplasts exhibited sustained cell division and formed microcalli when cultured in KPR medium supplemented with 0.5 mg $l^{-1}$ 2,4-D, 1.0 mg $l^{-1}$ NAA and 0.5 mg $l^{-1}$ zeatin using the agarose embedding procedure without feeder cells. Protoplast plating efficiencies ranged from 0.20 to 0.54%. Microcalli were transferred to MS medium supplemented with 2.0 mg $l^{-1}$ kinetin and 0.5mg $l^{-1}$ NAA for plant regeneration. The regeneration frequencies were 2 to 12%, depending on the cell suspension lines of Zhonghua 8. The plants were transferred to the glasshouse and were fertile.

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Studies on Proper Medium for Somatic Embryogenesis in Suspension Culture of Rehmania glutinosa and Encapsulation of Somatic Embryos (지황의 현탁배양에서 체세포배 형성에 관여하는 요인분석과 체세포배의 Encapsulation)

  • Park, Ju-Hyun;Park, Sang-Un;Chae, Young-Am
    • Korean Journal of Medicinal Crop Science
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    • v.3 no.2
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    • pp.100-106
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    • 1995
  • This study was conducted to find the factors affecting somatic embryogenesis in suspension culture of Rehmania glutinosa and investigate the possibility of artificial seed production by encapsulation of somatic embryos. Linsmeier-Skoog medium was appeared as proper for somatic embryogenesis. Sucrose with $3{\sim}5%$ as carbon sources was good for somatic embryogenesis, and both ammonium and nitrate nitrogen were necesary for normal somatic embryo production. BA with NAA or kinetin with NAA were better than the use of cytokinin alone for both somatic embryogenesis and numbers of somatic embryos. $AgNO_3$ as protectant for vitrification of seedlings in vitro culture had no harmful effect on somatic embryos. Sphericity of encapsulated seeds was good at 3% gel of sodium alginate but germination was better at 2.5% sodium alginate level. Artificial seeds were germinated and developed normal shoots and roots under in vitro condition.

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Plant Regeneration and Somatic Embryo Formation from Root-Derived Callus of Rice (벼 뿌리조직 유래의 캘러스로부터 체세포배 형성과 식물체 재분화)

  • 손재근;김경민;김종수
    • Korean Journal of Plant Tissue Culture
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    • v.22 no.3
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    • pp.143-148
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    • 1995
  • The competence of callus formation and plant regeneration from root derived callus was higher in japonica cultivars than those of Tongil-type cultivars of rice. A japonica type cultivars Yeongdeogbyeo, showed the highest capacity (13%) for plant regeneration from root calli of 6 cultivars tested. The callus induced from seed and root tissues maintained higher capacity for plant regeneration during 7 passages of subculture on N$_{6}$ solid media at 2-week intervals. The maximum frequency (2 x 10$^{5}$ mL) of round cells and their cell colonies showed about 24 days after suspension culture of root-derived callus in N$_{6}$ medium with lmg/L 2,4-D, 300mg/L casein hydrolysate, 10mM L-proline, 20g/L sucrose and 30g/L sorbitol. The frequency of somatic embryo formation in suspension cultures of root-derived callus increased with prolonged advance of subculture time from 30 to 90 days, but their regenerative capacities decreased.

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Micropropagation of Cassava by Suspension Culture Derived from its Nodal Explants (마디 절편의 현탁배양에 의한 카사바의 미세증식)

  • Yoon, Sil;Cho, Duck-Yee;Soh, Woong Young
    • Korean Journal of Plant Tissue Culture
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    • v.27 no.3
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    • pp.185-189
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    • 2000
  • For the micropropagation, node explants of cassava were cultured in liquid MS medium with various concentrations of cytokinins on a rotary shaker (100 rpm) for 2 weeks. The adventitious roots and shoots from the explants were differentiated more efficiently in liquid medium than in solid. But root formation was not inhibited in medium with BAP and kinetin at low concentration (>0.05 mg 1/sup -1/), while in medium added with BAP and zeatin at high level (<0.25 mg 1/sup -1/), it was inhibited by callus forming on cut end of the cuttings. However, all of plantlets grown in liquid medium for more than 2 weeks showed symptoms of hyperhydricity. The plantlets grown in liquid medium were transferred into culture bottles filled with fine sand or artificial soil (pitmoss:perlite:vermiculite, 1:1:1 v/v) wetted with half strength of Knop's solution. After transplanted to culture bottles, some of vitriscent leaves were defoliated and new leaves were normally formed from shoot apex. Most of plantlets (>95%) were hardened-off successfully only in culture bottles with fine sand, and grew into 3-5 cm seedlings possessing 4-6 nodes after 4 weeks. Thus, the mass propagation of cassava on medium containing cytokinin could be established based on the suspension culture using node explants.

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Thidiazuron-induced Shoot Formation of Hibiscus syriacus L. 'Honghwarang' by Suspension Culture (Thidiazuron이 무궁화 '홍화랑' 품종 액체 현탁 배양시 신초형성에 미치는 영향)

  • Kim, Eun Kyoung;Yoo, Yong Kweon;Kim, Ki Sun
    • Horticultural Science & Technology
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    • v.16 no.4
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    • pp.525-527
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    • 1998
  • This study was conducted to determine the optimum cultural condition and method for in vitro mass production of Hibiscus syriacus L. 'Honghwarang'. When callus induced in MS solid medium supplemented with 0.01 mg/L TDZ was cultured in liquid medium containing 0.01mg/L TDZ, callus growth and shoot primordia formation was most effective. Formed shoot primordia were regenerated into shoot in MS or 1/2 MS medium of growth regulator-free condition. Effects of mesh size, shaking speed on callus and shoot primordia formation were examined after 5 weeks. Callus and shoot primordia formation was formed most effectively at 10 mesh and 80 rpm shaking speed in liquid medium.

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Characteristics of Batch Cultures and Effects of Various Elicitors on Ginsenoside Production in Suspension Cultures of Panax Ginseng C.A. Meyer (고려인삼세포 현탁배양에서 회분배양 특성 및 Ginsenoside 생산에 대한 다양한 elicitors의 영향)

  • 유병삼;변상요
    • KSBB Journal
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    • v.16 no.6
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    • pp.620-625
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    • 2001
  • This study was examined to investigate the time course behaviors of cell growth and sucrose consumption, and effects of various elicitors on ginsenoside production in batch suspension cultures of Panax ginseng Meyer. Suspended cells reached to the stationary phase at 12 days after innoculation. The maximum cell concentration was 14.7 g-DCW/L at 17 days. The highest cell growth rate was 0.59 g-DCW/L d. The sucrose used as a sole carbon source was hydrolysed to glucose and fructose in 4 days, then quickly utilized until the middle-log phase and consumpted completely at 16 days. Various elicitors were app1ied at 8 days from inoculation which is the middle-log phase. Among the elicitors tested, jasmonic acid was the most efficient to increase the ginseneside production, which was 1.5 times higher than control.

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