• Journal of the Korean Geotechnical Society
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• v.17 no.1
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• pp.57-65
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• 2001

역사적 지진 발생 기록이 풍부하고 양산 단층 영향권 내에 있어 지진 발생 가능성이 높은 경주 지역을 대상으로 지진민감도 분석을 수행하였다. 지형적 특성을 고려한 지반조사와 현장 및 실내 시험을 통하여 대상 지역 지반의 지층 구성과 각 지층의 동적 특성을 분석 평가하고, 이를 토대로 한 지반 응답 해석을 수행하였다. 9개의 시험공을 시추하여 2개소의 크로스홀 시험과 7개소의 다운홀 시험을 실시하였고, 시추부지 포함 13개소에서 SASW 시험도 수행하였다. X선 회절 및 풍화도 분석과 공진주 시험 등의 실내시험도 병행하였다. 이러한 시험 결과를 바탕으로 El Centro 지진에 의한 지표면 최대 가속도를 등가 선형 부지응답해석을 통해 평가한 결과 붕괴방지 수준에서 0.158g~0.286g, 기능수행 수준에서 0.067g~0.116g의 분포를 보였고 퇴적층에 의하여 형성된 경주 시가지 분지 지형에서 증폭정도가 심하다는 것을 알 수 있었다. 해석 결과로 볼 때, 현행 건교부 기준 설계응답스펙트럼은 경우에 따라 지반 운동을 과소평가하고 지반 고유의 공진 현상을 고려하지 못하였다. 이러한 문제를 해결하기 위하여 개선된 지반 분류방법을 소개하였고 부지고유의 지반응답평가의 중요성에 대해 제시하였다.

• Tunnel and Underground Space
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• v.30 no.1
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• pp.1-14
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• 2020

Microseismic monitoring is utilized for the performance verification and safety management of the structure by detecting fine levels of damage. In order to construct a highly reliable microseismic monitoring system, the role of signal conditioner is critical. The signal conditioner helps with accurate data collection and precision control of the device, and performs additional functions such as signal conversion, linearization, and amplification. In this technical report, noise reduction signal conditioner suitable for mining sites was developed and reviewed for the purpose of implementing more precise monitoring by supplementing the previously developed microseismic monitoring system.

• Journal of Life Science
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• v.26 no.5
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• pp.503-508
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• 2016

Biosensors have been used as first-step monitoring tools to detect on-site samples in a simple and cost-effective manner. Numerous recombinant microbial biosensors have been exploited for monitoring on-site toxic chemicals and biological signals. Herein, a recombinant microbial biosensor was constructed for monitoring cadmium. The cadmium responding cadC regulatory gene and it’s promoter from Staphylococcus aureus was amplified through PCR, fused with the lacZ gene, and transformed into Escherichia coli BL21 (DE3) cells. In the presence of cadmium, the biosensor cells express β-galactosidase showing red color development with chlorophenol red β-galactopyranoside (CPRG) as the enzymatic substrate. The biosensor cells showed the best β-galactosidase activity after 3 hr induction with cadmium at pH 5 and a detection range from 0.01 μM to 10 mM cadmium with a linearity from 0.01 to 0.1 μM cadmium (y = 0.98 x + 0.142, R² = 0.98). Among the heavy metals, cadmium and lead showed good responses, tin and cobalt showed medium responses, and mercury and copper showed no responses. The biosensor cells showed good responses to several waste waters similar to buffer solution, all spiked with cadmium. The biosensor described herein could be applied for on-site cadmium monitoring in a simple and cost-effective manner without sample pretreatments.

• Journal of Korean Society of Coastal and Ocean Engineers
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• v.30 no.2
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• pp.51-60
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• 2018

This study was conducted to observe the effects of gravity and infra-gravity wave of detached submerged breakwater in the coast of Yeongnang-dong, Sokcho, as analyzing continuous wave data by performing field observations on the front area (W0) and rear area (W1, W2). Wave transmission coefficient ($K_t$) of submerged breakwater was analyzed in two parts, short-period wave (gravity wave) and infra-gravity wave. The wave energy reduction effect was analyzed and compared with the value of the design. In case of above wave height 2.0 m at the front area (W0) of the submerged breakwater, the short-period wave height at point W1 is reduced by about 65% and the short-period wave height at point W2 is reduced by about 59%. The depth of crest of submerged breakwater conducted in a sea area differs from the design, and the wave energy reduction effect is analyzed to be smaller than the design plan. The infra-gravity waves were amplified to 2.11 and 1.71 at the W1 and W2 points, respectively, and the wave height at W2 point was smaller than that at W1 point.

• Journal of the Korean Association of Geographic Information Studies
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• v.19 no.4
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• pp.17-35
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• 2016

Earthquake-induced disasters are often more severe in locations with soft soils than firm soils or rocks due to differences in ground motion amplification. On a regional scale, such differences can be estimated by spatially predicting subsurface soil thickness over the entire target area. In general, soil deposits are generally deeper in coastal or riverside areas than in inland regions. In this study, a coastal metropolitan area, Incheon, was selected to assess site effects and provide information on seismic hazards. Spatial prediction of geotechnical layers was performed for the entire study area within the GIS framework. Approximately 7,000 existing borehole drilling data in the Incheon area were gathered and archived into the GIS Database (DB). In addition, surface geotechnical data were acquired from a walkover survey. Based on the built geotechnical DB, spatial zoning maps of site-specific seismic response parameters were created and presented for use in a regional seismic strategy. Site response parameters were performed to determine site coefficients for seismic design over the entire target area and compared with each other. Site classifications and subsequent seismic zoning were assigned based on site coefficients. From this seismic zonation case study in Incheon, we verified that geotechnical GIS-DB can create spatial zoning maps of site-specific seismic response parameters that are useful for seismic hazard mitigation particularly in coastal metropolitan areas.

• Journal of Food Hygiene and Safety
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• v.29 no.1
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• pp.31-39
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• 2014

Norovirus causes acute gastroenteritis in all age groups and its food poisoning outbreaks are rapidly increasing in Korea. Reverse transcription-polymerase chain reaction (RT-PCR) is most widely used for the rapid detection of foodborne viruses due to high sensitivity. However, the false positive results of RT-PCR obtained against already inactivated viruses could be a serious drawbacks in food safety area. In this study, we investigated a method to yield true positive RT-PCR results only with alive viruses. To decompose the RNA genes from dead viruses, the enzymatic treatments composed of proteinse K and Ribonuclease A were applied to the sanitized and inactivated virus particles. Another aim of this study was to quantify the efficiencies of several major sanitizing treatments using real-time RT-PCR. Feline calicivirus (FCV) that belongs to the same Caliciviridae family with norovirus was used as a surrogate model for norovirus. The initial level of virus in control suspension was approximately $10^4$ PFU/mL. Most of inactivated viruses treated with the enzymatic treatment for 30 min at $37^{\circ}C$ were not detected in RT-PCR, Quantification results to verify the inactivation efficiencies of sanitizing treatments using real-time RT-PCR showed no false positive in most cases. We could successfully develope a numerical quantification process for the inactivated viruses after major sanitizing treatments using real-time RT-PCR. The results obtained in this study could provide a novel basis of rapid virus quantification in food safety area.

• Journal of Apiculture
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• v.32 no.1
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• pp.27-39
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• 2017

PCR-chip-based ultra-rapid multiplex PCRs for detection of six major infectious pathogens in honeybee were developed. The 6 kinds of major infectious pathogens in honeybee included Paenibacillus larvae causing American Foulbrood, Melissococcus plutonius causing European Foulbrood as bacteria, Ascosphaera apis (Chalkbrood), Aspergillus flavus (Stonebrood), Nosema apis and Nosema ceranae (Nosemosis) as fungi. The developed PCR-chip-based ultra-rapid multiplex PCR showed successful amplification for all six major pathogens in the presence of more than $10^3$ molecules. The time for confirming amplification (Threshold cycles; Ct-time) was about 7 minutes for two species, and about 9 minutes for four species. Total 40 cycles of PCR took 11 minutes 42 seconds and time for melting point analysis was 1 minute 15 seconds. Total time for whole PCR detection was estimated 12 minutes 57 seconds (40 cycles of PCR and melting point analysis). PCR-chip based ultra-rapid multiplex PCR using standard DNA substrates showed close to 100% accuracy and no false-amplification was found with honeybee genomic DNA. Ultra-rapid multiplex PCR is expected to be a fast and efficient pathogen detection method not only in the laboratory but also in the apiary field.

• Journal of the Korean Geotechnical Society
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• v.26 no.4
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• pp.27-36
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• 2010

In this study, a series of flat dilatometer tests are performed for Busan sand reconstituted in a large calibration chamber to evaluate the state parameter ($\Psi$). Experimental result shows that the horizontal amplification factor ($K_D/K_0$) is linearly related with state parameter in semi-logarithmic space, but the $K_D/K_0$ of OC specimen is smaller than that of NC specimen because of the horizontal residual stress by stress history of OC specimen. The relation between the normalized dilatometer modulus ($E_D/\sigma_m'$) and the state parameter is also linearly expressed in semi-logarithmic space, and the effect of stress history is relatively insignificant in this relation. However, the variation in $E_D/\sigma_m'-\Psi$ relation of NC state is slightly higher than that of OC state due to the effect of the stress level, and the correlation curve is descending with increase of confining stress. The comparison of test result with previous results of Ticino and Toyoura sands shows that the $E_D/\sigma_m'-\Psi$ relation of Toyoura sand is located on upper side than that of Busan and Tieino sands due to the effect of the higher compressibility, whereas the $K_D/K_0-\Psi$ relation of each sand is irregularly distributed.

• Journal of the Korean Society of Food Science and Nutrition
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• v.41 no.9
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• pp.1326-1330
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• 2012

This study was conducted to develop a loop-mediated isothermal amplification (LAMP) method for the detection of Clostridium difficile. The tested target gene was 16S ribosomal RNA. Five different LAMP primer sets were designed, and LAMP was performed. All primer sets targeting the 16S rRNA gene (BIP, FIP, B3, F3, LF, PF) were determined as positive in tcdA-positive, tcdB-postive ($A^+B^+$) and tcdA-negative, tcdB-negative ($A^-B^-$) Clostridium difficile strains. As the LAMP reaction took less than 80 min and did not require expensive machine such as thermocycler, it can be used as a rapid and simple detection method for foodborne pathogens.

• Journal of the Korea Institute of Information and Communication Engineering
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• v.7 no.5
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• pp.1019-1025
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• 2003

There are many kinds of seam trackers in the industrial welding field. We are proposed the contact seam tracking sensor applying strain gauges kind of contact sensor that mostly used in welding part now. For this seam tracking experiment, we made the strain gauges sensor by ourselves and tested how well the sensor tracks the seam. The experiment device consist of strain gauges sensor, amplifier circuit of strain gauges signal, saw wave generator, MOSFET power diving circuit and X-Y slide by moved DC motor. The tracking areas are X-Y planes(left, right, up and down) and the change of strain gauge resistance causes to move DC motor that connected to X-Y slide. As a result of experiment, we confirmed that the strain gauges sensor tracks a seam well, and X-Y slide DC motor was controlled by PWM control.