• Title/Summary/Keyword: 현미경

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Clinical Microscopy: Performance, Maintenance and Laser Safety (임상에서의 현미경: 작동, 유지보수 및 레이저 안전)

  • Lee, Tae Bok
    • Korean Journal of Clinical Laboratory Science
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    • v.51 no.2
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    • pp.125-133
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    • 2019

  • A microscope is the fundamental research and diagnostic apparatus for clinical investigation of signaling transduction, morphological changes and physiological tracking of cells and intact tissues from patients in the biomedical laboratory science. Proper use, care and maintenance of microscope with comprehensive understanding in mechanism are fully requested for reliable image data and accurate interpretation for diagnosis in the clinical laboratory. The standard operating procedure (SOP) for light microscopes includes performance procedure, brief information of all mechanical parts of microscopes with systematic troubleshooting mechanism depending on the laboratory capacity. Maintenance program encompasses cleaning objective, ocular lenses and inner optics; replacement and calibration of light source; XY sample stage management; point spread function (PSF) measurement for confocal laser scanning microscope (CLSM); quality control (QC) program in fluorescent microscopy; and systematic troubleshooting. Laser safety is one of the concern for medical technologists engaged in CLSM laboratory. Laser safety guideline based on the laser classification and risk level, and advisory lab wear for CLSM users are also expatiated in this overview. Since acquired image data presents a wide range of information at the moment of acquisition, well-maintained microscopes with proper microscopic maintenance program are impulsive for its interpretation and diagnosis in the clinical laboratory.

  • https://doi.org/10.15324/kjcls.2019.51.2.125 인용 PDF KSCI

Microphotographs and the Invisible Method of Specimen Preparation (현미경 사진, 그리고 감추어진 방법)

  • Sung, Han-Ah
    • Journal of Science and Technology Studies
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    • v.11 no.2
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    • pp.67-96
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    • 2011

  • Studies in scientific visualization have disclosed a visual chain among many scientific visualizations, which starts from the photographs that have many details to diagrams that enable scientists to make specific claims due to their abstract characters. Located at both ends of the visual chain, photographs and diagrams are regarded as being different kinds and having different characteristics. The photographs - or microphotographs to be precise - that this paper discusses, however, had been removed of unnecessary details and were prepared with carful attention to illustrative functions that diagrams usually perform. Following microphotographic changes in a Korean laboratory, this article shows that the knowledge of specimen preparation was transmitted from an American leading group to the Korean laboratory through a direct contact, and that with this, the technique of taking microphotographs of the laboratory was much improved. The specimen preparation method is, however, a tacit knowledge that is invisible in both group's papers. This article argues that the method of specimen preparation is the driving force that made the microphotographs act like carefully prepared diagrams and acquire objectivity.

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공초점 자가 간섭 현미경을 이용한 분해능 향상

  • Gang, Dong-Gyun;Gwon, Dae-Gap
    • Proceedings of the Korean Society Of Semiconductor Equipment Technology
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    • 2006.10a
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    • pp.1-4
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    • 2006

  • 공초점 자가 간섭 현미경은 측정 광학계에 복굴절 물질을 사용하여 반사된 빛에 대해 간섭 현상을 일으키는 것을 특징으로 한다. 이 간섭을 자가 간섭이라고 부르는데, 이는 시편의 한 점에서 반사되어 나온 빛이 간섭을 일으키기 때문에 붙여진 이름이다. 공초점 자가 간섭 현미경의 점 확산 함수는 종래의 공초점 현미경의 점 확산 함수와 자가 간섭의 곱으로 나타나며, 자가 간섭의 주기가 종래의 공초점 현미경의 점 확산 함수의 중심폭보다 작은 경우 점 확산 함수의 중심폭이 작아져서 수평 방향으로의 분해능이 향상되게 된다. 이러한 분해능 향상 정도를 측정하기 위하여 지름이 100nm 인 금으로 된 비드를 사용하였다. 측정된 결과는 전산모사한 결과와 잘 일치하며 공초점 자가 간섭 현미경에서 2배의 분해능 향상을 보여준다.

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The study of property on the michelson interferometric microscopy (마이켈슨 간섭계형 현미경의 특성 연구)

  • 김경신;권남익
    • Korean Journal of Optics and Photonics
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    • v.10 no.5
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    • pp.369-372
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    • 1999

  • We have constructed an interferometric microscopy using a Michelson interferometer and a He-Ne laser. The three dimensional image was obtained by the interference from the reflected signal by a sample surface and from the reflected signal by a mirror. The axial resolution obtained by Michelson interferometric microscopy is as good as that of the white-light interferometer, but the same fringe is obtained when optical path difference is half-wavelength. The image from Michelson interferometric microscopy was compared with the images from the various types of confocal microscopy.

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