• Journal of Food Hygiene and Safety
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• v.34 no.1
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• pp.22-29
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• 2019

Validamycin A is an aminoglycoside fungicide produced by Streptomyces hygroscopicus that inhibits trehalase. The purpose of this study was to develop a method for detecting validamycin A in agricultural samples to establish MRL values for use in Korea. The validamycin A residues in samples were extracted using methanol/water (50/50, v/v) and purified with a hydrophilic-lipophilic balance (HLB) cartridges. The analyte was quantified and confirmed by liquid chromatograph-tandem mass spectrometer (LC-MS/MS) in positive ion mode using multiple reaction monitoring (MRM). Matrix-matched calibration curves were linear over the calibration ranges (0.005~0.5 ng) into a blank extract with $R^2$ > 0.99. The limits of detection and quantification were 0.005 and 0.01 mg/kg, respectively. For validation validamycin A, recovery studies were carried out three different concentration levels (LOQ, $LOQ{\times}10$, $LOQ{\times}50$, n = 5) with five replicates at each level. The average recovery range was from 72.5~118.3%, with relative standard deviation (RSD) less than 10.3%. All values were consistent with the criteria ranges requested in the Codex guidelines (CAC/GL 40-1993, 2003) and the NIFDS (National Institute of Food and Drug Safety) guideline (2016). Therefore, the proposed analytical method is accurate, effective and sensitive for validamycin A determination in agricultural commodities.

• Korean Journal of Organic Agriculture
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• v.10 no.3
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• pp.87-99
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• 2002

The development of insecticidal soaps made by various fatty acids and organic control for insect by using of insecticidal soap(fatty acid salts) were employed. And the results were as follows: 1. To determine the input volume of potassium hydroxide for saponification of fatty acids, there were compared to individual acid value of fatty acids. in case of coconut fatty acids for saponification was 266.3 mg of potassium hydroxide(266 g/1kg of fatty acid) was calculated. 2. To make the 25% soap content by coconut fatty acids, there was required for the 266g of potassium hydroxide and 3,459 liter of soft water. Then the liquor of soap was 4,644 liter. 3. The progress of insecticidal soap made by fatty acids was accomplished indirect heating and stirring reactor (1)to make the volume of potassium hydroxide solution and warming up(90$^{\circ}$) (2)input volume of individual fatty acid (3)more than 30 min stirring reaction (4)cooling progress. 4. Insecticidal value of insecticidal soap was observed more than 92% the dilution of 50 dilution solution in consecutive 5 days of 2 treatments of the 25% soap made by coconut fatty acids on the red pepper. And insecticidal value of insecticidal soap was observed more than 94% the 100 dilution solution in consecutive 5 days of 3 treatments of the 25% soap made by coconut fatty acids on red pepper and cabbage. 5. The treatment of two times of 25% soap made by coconut fatty acids at the 50 dilution solution and 100 dilution solution in spider mite on red bean has 100% insecticidal value. 6. There was no observation phytotoxic sypmtons on red pepper in field, except for 25% soap made by carprylic acid. 7. Over the two times over 0.1% in addition of isopropyl alcohol was to improvement the insecticidal effect, but there was no effect in addition of diatomaceous earth.

• The Korean Journal of Nuclear Medicine Technology
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• v.15 no.2
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• pp.26-29
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• 2011

Purpose: The general test method of the Korean Pharmacopeia specifies the test method on the clauses of quality control after manufacturing. According to KFDA Guidance for Medicines, standards of residual solvents regulates the maximum permissible dose of acetonitrile as 400 ppm, ethanol as 5,000 ppm, and acetic acid as 5,000 ppm. This study aims at identifying the type of resiual solvents in the final [F-18]FDG vial of an automatic synthesizer and measure its residual quantity. Materials and Methods: The center carried out residual solvents test of [F-18]FDG injection using Agilent Technologies 7890A with a Flame Ionization Detector. The column of Agilent Technologies 7890A used in measuring of residual solvents was CP WAX column ($30m{\times}0.53mm{\times}1.0{\mu}m$) and analysis condition was split mode 1:1 at the initial temperature $70^{\circ}C$ which was increased $20^{\circ}C/minute$ after two minutes and maintained at the final $140^{\circ}C$ for two minutes. The analysis method was as following: Firstly, ethanol-acetonitrile-acetic acid mixture was classified into four types of concentration (250-25-250 ppm, 1,000-100-1,000 ppm, 3,000-300-3,000 ppm, and 6,000-600-6,000 ppm), and $1.0{\mu}L$ of each type of concentration was injected into gas chromatography followed by an analysis of its peak domain. Then, a calibration-curve by the external standard method was drawn based on the analysis result. Results: While ethanol and acetonitrile were detected in TRACERlab MX, FASTlab had additional acetic acid. The residual quantity of the ethanol-acetonitrile-acetic acid mixture evaluated using the calibration-curve was average 72 ppm ethanol, 54 ppm acetonitrile, and 1030 ppm acetic acid for FASTlab, whereas average 439 ppm ethanol and 79 ppm acetonitrile for TRACERlab MX. This indicated that both of them were within the maximum permissible dose. Conclusion: Solvent residues in the [F-18]FDG injection were all within maximum permissible doses and proper to be used to examine a patient. The result indicated that types and quantities of solvent resides of radioactive pharmaceuticals vary depending on the automatic synthesizer.

• Journal of Korean Society of Transportation
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• v.18 no.1
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• pp.17-26
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• 2000

U-turns are allowed widely at the intersections by local police department while the left-turn Phases have been gradually Prohibited. However, any strategies for U-turn movements at signalized intersections are unavailable. Therefore, the Purpose of this study is to Propose the efficient operational method of Urban arterial adopting U-turn strategies. Four alternatives are evaluated they are, 1) U-turn movements are allowed at the adjacent intersection with exclusive U-turn lane while the major or the minor approach is Prohibited, 2) U-turn movements are allowed at the adjacent mid-block Pedestrian crossing with exclusive U-turn lane while the major approach is Prohibited. 3) U-turn movements are allowed at the adjacent mid-block Pedestrian crossing with exclusive U-turn lane while the minor approach is prohibited and 4) Comparative one between alternative 3 and 4. From the results of this study, it concludes that the method of U-turn movements allowed at the adjacent mid-block pedestrian crossing with exclusive U-turn lane is the most effective strategy among those alternatives. The strategies of alternative 1 and 4 are Proposed by the boundary based on the major through and left-turn volumes and the minor left-turn volume.

• Journal of Applied Biological Chemistry
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• v.55 no.4
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• pp.235-239
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• 2012

While cultivating crops, it is important to predict the biological half-lives of applied pesticides to ensure the safety of agricultural products. Dissipation patterns of the triazole fungicides, such as diniconazole and metconazole, during the cultivation of apple were established by utilizing the dissipation curve. As well as, the biological half-lives of the pesticides in apples were calculated using the residue amounts of them. The apples were harvested from 0 to 14 days after spraying diniconazole (WP) and metconazole (SC) at a recommended and three times of the recommended dose. Initial concentrations of diniconazole in apple were 0.09 and 0.15 mg/kg at a recommended and three times of the recommended dose, respectively, which were below MRL 1.0 mg/kg established by KFDA. The equations of biological half-life were $C_t=0.0811e^{-0.179x}$(half life: 3.9 days) and $C_t=0.1451e^{-0.148x}$ (half life: 4.7 days), respectively. In case of metconazole, initial concentrations in apple were 0.10 and 0.25 mg/kg, below MRL 1.0mg/kg, and biological half-life equations were $C_t=0.0857e^{-0.055x}$ (half life: 12.6 days) and $C_t=0.2304e^{-0.052x}$ (half life: 13.3 days), respectively. Therefore, when triazole fungicides were applied during the cultivation of apple, the biological half-life need to be calculated with the optimal equation model.

• Progress in Medical Physics
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• v.22 no.2
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• pp.99-105
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• 2011

The purpose of this study is to evaluate the accuracy of IMRT in our clinic from based on TG119 procedure and establish action level. Five IMRT test cases were described in TG119: multi-target, head&neck, prostate, and two C-shapes (easy&hard). There were used and delivered to water-equivalent solid phantom for IMRT. Absolute dose for points in target and OAR was measured by using an ion chamber (CC13, IBA). EBT2 film was utilized to compare the measured two-dimensional dose distribution with the calculated one by treatment planning system. All collected data were analyzed using the TG119 specifications to determine the confidence limit. The mean of relative error (%) between measured and calculated value was $1.2{\pm}1.1%$ and $1.2{\pm}0.7%$ for target and OAR, respectively. The resulting confidence limits were 3.4% and 2.6%. In EBT2 film dosimetry, the average percentage of points passing the gamma criteria (3%/3 mm) was $97.7{\pm}0.8%$. Confidence limit values determined by EBT2 film analysis was 3.9%. This study has focused on IMRT commissioning and quality assurance based on TG119 guideline. It is concluded that action level were ${\pm}4%$ and ${\pm}3%$ for target and OAR and 97% for film measurement, respectively. It is expected that TG119-based procedure can be used as reference to evaluate the accuracy of IMRT for each institution.

• The Korean Journal of Nuclear Medicine Technology
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• v.18 no.1
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• pp.10-18
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• 2014

Purpose: Among different PET/CT devices which are composed of same PET model but different CT models, SUV, usually used for quantitative evaluation, was measured to assess the accuracy of follow up scans in different PET/CT and confirm that interequipment compatibility is useful in arranging the PET/CT exam appointment. Materials and Methods: Using ACR PET Phantom, PET NEMA IEC Body Phantom, SNM Chest Phantom and Ge-68 cylinder Phantom, $SUV_{mean}$ and $SUV_{max}$ was measured by 3 different models of PET/CT (Discovery 690, Discovery 690Elite and Discovery 710, GE) made in same company. ANOVA was used to evaluate the significant difference in the result. Results: In the result, the average of $SUV_{max}$ was D690 (25 mm-1.82, 16 mm-1.75, 12 mm-1.73, 8 mm-1.44), D690E (25 mm-1.76, 16 mm-1.92, 12 mm-1.78, 8 mm-1.55) and D710 (25 mm-1.84, 16 mm-1.89, 12 mm-1.77, 8 mm-1.61) in ACR Phantom, D690 (25 mm-2.26, 16 mm-2.25, 12 mm-1.92, 8 mm-1.85), D690E (25 mm-2.45, 16 mm-2.25, 12 mm-2.05 8 mm-1.91) and D710(25 mm-2.49, 16 mm-2.20, 1 2mm-2.30, 8 mm-2.05) in PET NEMA IEC Body Phantom, D690-1.04, D690E-1.10 and D710-1.09 in SNM Chest Phantom and D690-0.81, D690E-0.81, D710-0.84 in Ge-68 cylinder Phantom. The differences between average SUV of 4 phantoms were $SUV_{mean}$-1.87%, $SUV_{max}$-2.15%. And also as a result of ANOVA analysis, there was no significant difference statistically. Conclusion: If different models of PET/CT have same specification of PET system, there was no significant difference in $SUV_{mean}$ and $SUV_{max}$ even though they have different CT system. And also differences of $SUV_{mean}$ and $SUV_{max}$ in phantom images were under 5% which many manufacturers recommend. Therefore, follow up scan will be possible using different PET/CT if it has same specification of PET system with the previous PET/CT. This information will enable the accurate comparative analysis when conducting follow up scans and be helpful to schedule PET/CT exam more effectively.

• Journal of Korean Society of Coastal and Ocean Engineers
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• v.18 no.2
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• pp.137-146
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• 2006

This is the second of a two-part paper which describes comparison of reliability design methods by application to Donghae Harbor Breakwaters. In this paper, Part 2, we deal with sliding of caissons. The failure modes of a vertical breakwater, which consists of a caisson mounted on a rubble mound, include the sliding and overturning of the caisson and the failure of the rubble mound or subsoil, among which most frequently occurs the sliding of the caisson. The traditional deterministic design method for sliding failure of a caisson uses the concept of a safety factor that the resistance should be greater than the load by a certain factor (e.g. 1.2). However, the safety of a structure cannot be quantitatively evaluated by the concept of a safety factor. On the other hand, the reliability design method, for which active research is being performed recently, enables one to quantitatively evaluate the safety of a structure by calculating the probability of failure of the structure. The reliability design method is classified into three categories depending on the level of probabilistic concepts being employed, i.e., Level 1, 2, and 3. In this study, we apply the reliability design methods to the sliding of the caisson of the breakwaters of Donghae Harbor, which was constructed by traditional deterministic design methods to be damaged in 1987. Analyses are made for the breakwaters before the damage and after reinforcement. The probability of failure before the damage is much higher than the allowable value, indicating that the breakwater was under-designed. The probability of failure after reinforcement, however, is close to the allowable value, indicating that the breakwater is no longer in danger. On the other hand, the results of the different reliability design methods are in fairly good agreement, confirming that there is not much difference among different methods.

• Journal of Embryo Transfer
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• v.21 no.3
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• pp.207-216
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• 2006

Vaginal cytology was examined in 12 Shih-tzu bitches to establish the accurate basic data for estimate to the optimal mating time and ovulation time. The mean duration of proestrus and estrus were $9.09{\pm}0.83\;(mean{\pm}SD)$ days and $7.36{\pm}0.47$ days in pregnant bitches. The gestational length in the 12 pregnant bitches was $65.2{\pm}0.5$ days in pregnant bitches when Day 0 was timed from the first day of male acceptance. Characteristic features of vaginal cytology during the estrous cycle were the high proportion of large intermediate cell, superficial cell, anuclear cell and erythrocyte in proestrus, superficial cell, anuclear cell and erythrocyte in estrus, and parabasal cell, small and large intermediate cell and leukocyte in diestrus, respectively. Cornification index (CI) was the high proportion in proestrus and estrus, then it decreased in diestrus and anestrus. When Day 0 was timed from the day of the first male acceptance, the CI peak was Day 2 and maintained above 80% between Day -4 and Day 6 during 11 days, and above 90% between Day -1 and Day 5 during 7 days. In relationship between CI and reproductive hormones, CI showed peak at the first day after plasma estradiol-$17{\beta}$ concentration peak and plasma progesterone concentration was first increased above 4.0 ng/ml at Day 0 which was the first day after CI peak. In conclusion, ovulation in Shih-tzu bitches occurred at the first day after CI peak. Vaginal cytology is the simple and reliable method for estimating estrous cycle, optimal breeding time and ovulation time in Shih-tzu bitches.

• The Korean Journal of Pesticide Science
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• v.12 no.2
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• pp.148-154
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• 2008

This study was carried out to investigate the residue patterns of two insecticides, lambda-cyhalothrin and deltamethrin, commonly used for lettuce, under greenhouse conditions. The pesticides were sprayed with dilution of recommended and doubled doses onto lettuce. Their detection limits were $0.001\;mg{\cdot}kg^{-1}$ and mean recoveries at the fortification levels of 0.2 and $1.0\;mg{\cdot}kg^{-1}$ were from 101.17 to 104.25 and from 99.70 to 103.77%, respectively. The pesticides were gradually decreased in lettuce with time. Biological half-lives of lambda-cyhalothrin and deltamethrin were 1.7 and 1.4 days at the recommended dose and 1.8 and 1.4 days at the doubled dose, respectively. Initial residue amounts of lambda-cyhalothrin and deltamethrin at the recommended and doubled doses exceeded their MRLs. However, the residue levels of the pesticides in the crop sampled at harvest were less than their MRLs. The ratios of the estimated daily intake (EDI) to acceptable daily intake (ADI) by intake the crop harvested 10 days after spraying were less than 1% of their ADIs.