• Korean Journal of Human Ecology
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• v.22 no.6
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• pp.687-699
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• 2013

This study was carried out to investigate the antifungal activity and house dust mite repellent effect of cotton and silk fabrics dyed with Juniperus chinensis heartwood extracts by hot-water and methanol. The chemical composition of Juniperus chinensis heartwood extracts by hot-water and methanol was analyzed by GC-MS. In the case of antifungal activity, dyed fabrics with hot water extract had no effect against chaetomium globosum fungus. But showing no growth in fabrics dyed with methanol extract, dyed fabrics with methanol extract showed very excellent antifungal activity. Silk fabric dyed with methanol extract showed excellent house dust mite repellent effect of 94.3%~96.0% against dermatophagodes farinae. The composition of Juniperus chinensis heartwood extracts from hot-water and methanol was different. There were no terpene and its analogue peaks in hot-water extract. However there were terpene and its analogue peaks in methanol extract. From these GC-MS results, terpene was identified in methanol extract of Juniperus chinensis heartwood. Efficient ingredient of antifungal activity and house dust mite repellent effect was assumed terpene.

• Journal of Korea Foresty Energy
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• v.24 no.2
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• pp.24-30
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• 2005

Four compounds, (+)-catechin (1), (-)-epicatechin (2), myricitrin (3), and hinokiflavone (4), were isolated from the needles of Juniperus occidentalis Hook. The structures of the isolated compounds were established by NMR and MS spectrometer. The antioxidant activity of the isolated compounds was determined by measuring free radical scavenging activity with DPPH and the results indicated that compound 1, 2, and 3 showed better activity than the controls, while compound 4 had weak activity compared with ${\alpha}-tocopherol$, BHT, and curcumin.

• Journal of Korean Society of Forest Science
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• v.13 no.1
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• pp.1-24
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• 1971

In order to elucidate the feasibility to identify the plant species through the photographic tone this study was made. In this study the author made photographs of Juniperus chinensis L., and Populus glandulosa Uyeki, with the panchromatic film using either yellow filter or red filter in different seasons respectively. The author analyzed the value of tone variation at the level of stereoscopic view of the same photographs by using tone scale and Automatic Micro-Photo-Densitometer. The results obtained are summarized as follows: A. Tone scale reading 1. The tone of Populus glandulosa Uyeki was darker than that of Juniperus chinensis L. in a photograph. 2. Regardless of the tree species, tone of photographs obtained with yellow filter was darker than that with red filter. 3. Along the progresses of seasons, the photographic tone was changed. That is, from the spring to the summer it showed darker and than, from the summer to winter it changed lighter. 4. During winter and spring the discrimination between the both species of trees can be easily made by stereoscopic view whether there are leaves on the tree or not rather than by tone observation. 5. Regardless of tree species, variation of tone due to the age was noticed. The older trees have darker tone than the younger one. 6. It is recognized that the yearly difference depends on insolation quantities. 7. The highest reflex light-waves were in between $600m{\mu}$ and $660m{\mu}$ for both species of trees. B. Density reading 1. For the density reading, there was the same tendency as in tone scale reading. 2. The changes of peaks of the scanning curves with Populus glandulosa Uyeki is smoother and takes place in lower position than with Juniperus chinensis L. 3. The scanning curves on the 20th May was smoothest, and change of peaks increased gradually according to the season progresses. 4. In relation to the types of filter the photographs with yellow filter showed less changes of peaks then that with red filter.

• Journal of the Society of Cosmetic Scientists of Korea
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• v.30 no.1
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• pp.63-71
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• 2004

The human skin is constantly exposed to environmental irritants such as ultraviolet, smoke, chemicals. Free radicals and reactive oxygen species (ROS) caused by these environmental facts play critical roles in cellular damage. These irritants are in themselves damaging to the skin structure but they also participate the immensely complex inflammatory reaction. The purpose of this study was to investigate the skin cell protective effect of Juniperus chinensis xylem extract on the UV and SLS-induced skin cell damages. We tested free radical and superoxide scavenging effect in vitro. We found that Juniperus chinensis xylem extracts had potent radical scavenging effect by 98％ at 100 $\mu\textrm{g}$/mL. Fluorometric assays of the proteolytic activities of matrix metalloproteinase-l(MMP-1, collagenase) were performed using fluorescent collagen substrates. UV A induced MMP-1 synthesis and activity were analyzed by enzyme-linked immunosorbent assay (ELISA) and gelatin-based zymography in skin fibroblasts. The extract of Juniperus chinensis showed strong inhibitory effect on MMP-1 activities by 97％ at 100 $\mu\textrm{g}$/mL and suppressed the UVA induced expression of MMP-1 by 79％ at 25 $\mu\textrm{g}$/mL. This extract also showed strong inhibition on MMP-2 activity in UVA irradiated fibroblast by zymography. We also examined anti-inflammatory effects by the determination test of proinflammatory cytokine, interleukin 6 in HaCaT keratinocytes. In this test Juniperus chinensis decreased expression of interleukin 6 about 30％. Expression of prostaglandin E$_2$, (PGE$_2$) after UVB irradiation was measured by competitive enzyme immunoassay (EIA) using PGE$_2$ monoclonal antibody. At the concentrations of 5-50 $\mu\textrm{g}$/mL of the extracts, the production of PGE$_2$ by HaCaT keratinocytes (24 hours after 10 mJ/$\textrm{cm}^2$ UVB irradiation) was significantly inhibited in culture supernatants (p〈0.05). The viability of cultured HaCaT keratinocytes was significantly reduced at the doses of above 10 mJ/$\textrm{cm}^2$ of UVB irradiation, but the presence of these extracts improved cell viability comparing to control after UVB irradiation. We also investigated the protective effect of this extract in sodium lauryl sulfate (SLS)-induced irritant skin reactions from 24 hour exposure. Twice a day application of the extract for reducing local inflammation in human skin was done. Irritant reactions were assessed by various aspects of skin condition, that is, erythema (skin color reflectance) and transepidermal water loss (TEWL). After 5 days the extract was found to reduce SLS-induced skin erythema and improve barrier regeneration when compared to untreated symmetrical test site. In conclusion, our results suggest that Juniperus chinensis can be effectively used for the prevention of UV and SLS-induced adverse skin reactions such as radical production, inflammation and skin cell damage.

• Journal of the Society of Cosmetic Scientists of Korea
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• v.30 no.2
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• pp.227-233
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• 2004

Chronic exposure to solar radiation, particularly ultraviolet (UV) light, causes a variety of adverse reactions on human skin, such as sunburn, photoaging and photocarcinogenesis. Free radicals and reactive oxygen species (ROS) caused by UV exposure or other environmental facts play critical roles in cellular damage. And, repeated-UV irradiation activated the expression of the matrix metalloproteinase (MMP) and induced skin irritation. Therefore, the development of effective and safe photoprotectants that can reduce and improve the skin damage has been required. The purpose of this study was to investigate the photo-protective effect of several chinese medical plants (Juniperus chinensis) on the UV -induced skin cell damages. We tested free radical and superoxide scavenging effect in vitro. Fluorometric assays of the proteolytic activities of MMP-1 (collagenase) were performed using fluorescent collagen substrates. UVA induced MMP-1 synthesis and activity were analyzed by enzyme-linked immunosorbent assay (ELISA) and gelatin-based zymography in skin fibroblasts. We also examined anti-inflammatory effects by the determination test of proinflammatory cytokine, interleukin 6 in HaCaT keratinocytes. Expression of prostaglandin E$_2$ (PGE$_2$) after UVB irradiation was measured by competitive enzyme immunoassay(EIA) using PGE$_2$ monoclonal antibody. In the human skin we tested anti-irritation effect on the SLS-induced damage skin after appling the extract containing emulsion. We found that Juniperus chinensis extract had potent radical scavenging effect by 98% at 100$\mu\textrm{g}$/mL. The extract of Juniperus chinensis showed strong inhibitory effect on MMP-1 activities by 97% at 100 $\mu\textrm{g}$/mL and suppressed the UVA induced expression of MMP-1 by 79% at 25$\mu\textrm{g}$/mL. This extract also showed strong inhibition on MMP-2 activity in UVA irradiated fibroblast by zymography. In the test of proinflammatory cytokines of human keratinocytes Juniperus chinensis extract decreased expression of interleukin 6 about 30%. The amount of PGE$_2$ by HaCaT keratinocytes was significantly increased at the doses of above 10 mJ/$\textrm{cm}^2$ of UVB (p < 0.05). At the concentrations of 3.2-25$\mu\textrm{g}$/mL of this extract, the production of PGE$_2$ by HaCaT keratinocytes (24 h after 10mJ/$\textrm{cm}^2$ UVB irradiation) was significantly inhibited in culture supernatants (p < 0.05). In SLS-induced skin irritation model in vivo, we found to reduce skin erythema and improve barrier recovery after appling Juniperus chinensis extract containing emulsion when compared to irritated non-treated and placebo-treated skin. Our results suggest that Juniperus chinensis extract can be effectively used for the prevention of UV and SLS-induced adverse skin reactions and applied as anti-aging and anti-irritation cosmetics.

• Journal of Korean Society of Forest Science
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• v.102 no.4
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• pp.587-600
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• 2013

Juniperus chinensis forest were classified into 1 community group, 2 communities, 4 groups, 6 subgroups, and 7 vegetation units by phytosociological analysis(the method of ZM schools) with 79 vegetation data in south korea. By NMS analysis it appeared at first axis (13.8%) and second axis (69%) and comprehensive explanatory power was 31%. It was shown clearly in Donggang region, the East sea region and the Ulleungdo region. In INSPAN(Indicator species Analysis) of vegetation unit, 89 species were analyzed significantly. According to result of species diversity, evenness, dominance value, interspecific-competition and other related analyses for vegetation unit, the species diversity of vegetation unit 4 showed the lowest value 0.667, while the value of vegetation unit 6 showed the highest value 1.174. The dominance value of vegetation unit 7 showed the lowest value 0.163 and most vegetation units are on about 0.7, while the value of vegetation unit 1 showed highly above 0.8.

• Korean journal of applied entomology
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• v.38 no.1
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• pp.15-17
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• 1999

An Olethreutine species Eucoegenes ancyrota (Meyrick), which tie together 2-3 leaves of Ternstroemia japonica Thunb. and feed it, is reported for the first time from Korea with morphological character of larva and adult, and a brief biology.

• Journal of the Korean Society of Clothing and Textiles
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• v.41 no.1
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• pp.131-140
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• 2017

Uniform nanofibers of polyurethane with different content of Juniperus Chinensis extracts (JCE) were successfully prepared by the electrospinning method. We investigated physiochemical properties of prepared compound nanoweb according to various concentrations of Juniperus Chinensis extracts using a Fourier transform infrared (FT-IR) spectrometer, X-ray diffractometer (XRD), thermogravimeter (TGA), and differential scanning calorimeter (DSC). The antibacterial activity of the JCE loaded PU nanofiber was conducted using the disk diffusion test against Gram-positive and Gram-negative bacteria. JCE was induced in the infrared spectra in the absorption band of PU/JCE nanowebs at $3,300cm^{-1}$, $2,960cm^{-1}$, $1,400-1,600cm^{-1}$, and $1,050cm^{-1}$. Thermal stability decreased with increasing JCE content in the PU/JCE nanowebs. The DSC curve of the PU nanoweb shows an endotherm peak at $420^{\circ}C$; in addition, the peak also became smaller and broader with increasing JCE content. The diffraction intensities of PU observed at 2 theta of $20^{\circ}$ decreased with the increasing amount of JCE in the compound nanoweb. In addition, the crystal intensities of the compound nanowebs also decreased along with the JCE content. Structural analysis indicates that JCE and PU are miscible. Juniperus Chinensis incorporated PU nanofibers demonstrated excellent antibacterial properties against both Gram-positive and Gram-negative bacteria.

• Korean Journal of Human Ecology
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• v.21 no.5
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• pp.989-1004
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• 2012

The purpose of this study was to investigate the characteristics of the pigment and the dyeability of juniperus chinensis needles, berry, bark and heartwood extracts using distilled water, methanol, normal butanol and ethyl acetate as a solvent. Ultraviolet-visible absorption spectrum bands of the extracts were measured at around 280nm to 320nm in all the solvent extracts. The maximum absorption wavelength was able to determine tannin. All the solvent extracts except for distilled water extracts were able to confirm the presence of chlorophyll. Infrared absorption spectra (FT-IR) of all parts of the extracts showed broad absorption bands of OH due to phenolic-OH, benzene CH peak of phenol chemical structure, ether-based stretching vibration peak and the peak of flavonoid compounds that appeared in all the solvent extracts. The yield of juniper needles and heartwood in distilled water and methanol extraction were effective. Extraction of berry yields in distilled water was also effective. The yield of ethanol extraction from the bark showed better efficiency. As a result of using distilled water for the dye solution, the color of the fabrics dyed with all extracts of the needles and Y series berries generally showed light Y progression with a strong red tinge. By using a mixture of 20% ethanol and 80% distilled water for the dye solution, the color of the fabrics dyed with needles and berry extracts showed Y series dominantly. The color of the fabrics dyed with Bark and heartwood extracts were dominantly R series.

• Korean journal of applied entomology
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• v.19 no.1 s.42
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• pp.39-44
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• 1980

The outbreak of the pear rust caused by Gymnosporangium haraenum SYDOW has increased gradually since 1973, and amounted to 60.3 percent infection of leaves in 1975. But it has reduced due to regulation of juniper plantings by Plant Protection Law enacted in 1974. The teliospores of C. haraeanum germinated from middle of April to middle of May under natural condition. The germination of teliospores was observed 5 day earlier under laboratory condition with the artificial rainfall than that of natural condition. It was required considerable rainfall and longer than 3hr rainy period to germinate teliospores after being matured. Infection of rear leaves took place in the rain or just after the rain, but not after 24hr. The incubation period of G. haraeanum on the leaf was 9 to 10 days. In case that the distance between orchard and juniper trees was less than loom, almost all of the pears were infected, but the chance oft infection reduced in proportion as the distance increased. No infection occurred at the distance of further than 2000m.