• Proceedings of the Korean Society for Applied Microbiology Conference
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• 1978.10a
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• pp.205.1-205
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• 1978

일반적으로 쌀막걸리 중에는 풍미에 관여한다고 생각되는 핵산관련물질이 핵산분해효소인 PDase (phosphodiesterase) 및 PMase (phospomonoesterase) 등의 작용을 받아 대부분이 분해되는 것으로 알려져 있다. 따라서 저자등은 쌀막걸리 제조공정을 통하여 핵산관련물질의 분포와 그와 관련된 핵산 분해효소에 대한 실험을 하였다. 본보에서는 제국중 핵산관련물질과 핵산관련물질을 분해하는 효소의 경시적 변화를 조사하였으며 이들 분해효소의 효소학적 성질을 검토하여 몇가지 결과를 얻었으므로 보고하는 바이다.

• Korean journal of food and cookery science
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• v.3 no.1
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• pp.71-77
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• 1987

This study was to investigate the synergistic taste effect between monosodium glutamate(MSG) and 5'-ribonucleotides consisted of disodium 5'-inosinate (IMP) and 5'-guanylate (GMP) as 1:1 ratio. Solvent was distilled water. Sensory evaluation with 10 panelists was performed by using ratio scaling method (magnitude estimation). The results were as follows: 1) Taste intensities were increased, as nucleotides content to MSG increased. 2) Multiple regression analyses were carried out with the taste intensity data as a function of nucleotides content at three concentrations of seasonings, 0.025%, 0.05% and 0.1%. 3) Predicted taste intensities ($Y_P$) were calculated from the regression equation. Also taste intensity ratios ($Y_{TR}$)-$Y_{TR}$=$Y_P$/taste intensity of MSG only-were calculated. 4) The taste intensity ratios ($Y_{TR}$) at three concentrations of seasonings in the same nucleotides contents showed about the same. Therefore, instead of above regression equations, only one multiple regression equation expressing $Y_P$ of nucleotides seasonings could be determined, as functions of nucleotides content and seasoning concentration.

• Proceedings of the Korean Society of Fisheries Technology Conference
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• 2000.05a
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• pp.74-75
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• 2000

핵산의 생합성은 salvage합성이 우선되며 이 경우 섭취된 음식물유래의 핵산등이 이용되므로 섭취되는 핵산의 영양학적 가치는 매우 높다(송영정사, 1995). Van Buren이나 Kulkarni등은 mouse에의 핵산 경구투여가 면역능을 개선하여 세균감염에 대한 방어에 유효하다고 보고하였고(Van Buren C.T et al, 1983, Kulkarni A.D et a 1986), 이들의 연구가 계기가 되어 섭취 핵산의 유용성에 대한 연구보고가 계속적으로 행하여지고 있는데 그 생리활성을 살펴보면 세포부활작용(Matsunaga et al, 1987) 면역증강작용(Frederic B. Rudolph, 1994) 과산화지질 형성억제작용(Uauy R et al 1985), 장내균총 및 지질대사의 개선작용(Uauy R, 1994, Angel Gil et al, 1986)등이다 본 연구에서는 부산물로 폐기되어지는 참치정소를 이용하여 핵산복합물질을 분리·정제하는 방법 및 그 추출물의 생리활성을 연구하므로써 새로운 기능성 소재로서의 이용가능성을 탐색하였다. (중략)

• YAKHAK HOEJI
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• v.21 no.2
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• pp.70-80
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• 1977

본고에서는 핵산 생합성과 Salvage patheway (Preformed Purine Utilization), De novo Pyrimidine Biosynthesis, Slavage patheway (Preformed Pyrimidine Utilization), Purine Base 및 Purine Nucleoside와 비슷한 구조를 가진 핵산 대사 길항제에 대한 내용으로 구성되어 있다.

• KSBB Journal
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• v.11 no.1
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• pp.15-21
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• 1996

Purification of hyaluronic acid produced by Streptococcus equi was carried out to obtain clinical grade hyaluronic acid. The removal method of the bacteria was selected as filtration because filtration was the most effective method in removing impurities such as protein and nucleic acid of the fermentation broth. The removal efficiencies of protein and nucleic acid of hyaluronic acid solution were increased to 75% and 67%, respectively, by filtration with adding 0.6% of activatied carbon and 1.0% colite. Hyaluronic acid solution was precipitated by mixing with 2 volumes of ethanol. Effects of pH and conductivity on ethanol preciptation of hyaluronic acid were investigated. Protein and nucleic acid of hyaluronic acid were remained almost constant regardless of pH and conductivity, and the recovery of hyaluronic acid was optimum as about 85% at pH 7 and l00mS of conductivity Protein of hyaluronic acid was completly removed by three serial filtration and ethanol precipitation, however, nucleic acid was not removed. Hyaluronic acid solution was passed through a column of Duolite A7 to remove its nucleic acid, where 65% of nucleic acid was removed at pH 7 and 40mS of conductivity. The residual nucleic acid of hyaluronic acid solution was completly removed by treatment of 0.2% hydroxyapatite and the clinical grade hylauronic acid could be obtained.

• Food Industry
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• s.42
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• pp.20-26
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• 1978

• 한국해양학회지
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• v.25 no.3
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• pp.145-150
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• 1990

An analysis of collected data on components of dissolved organic phosphorus (DOP) and DOP is made to search for important components of DOP pool and their implications in phosphorus (P) cycling. The significance of dissolved nucleic acids (D-NA) apparently tends to increase with increasing trophic status of oceanic waters. Interestingly the sum of all 5-nucleotide and D-NA seems to dominate the DOP pool. Thus, materialization of D-NA could be a significant pathway of P cycling in surface oceanic waters and it might be of great importance in lakes where P limits primary production. Processes related to death of Microbes are responsible for D-NA and DOP production in surface waters, and incomplete digestion of preys by grazers seems to be an important mechanism in D-NA production.

• Proceedings of the Korean Society for Applied Microbiology Conference
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• 1977.10a
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• pp.200.1-200
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• 1977

핵산관련질의 구조식 및 핵산계 조미료로 사용되고 있는 $5'-IMPㆍNa_2$ 및 $5'-GMPㆍNa_2에$ 대한 업학적성질과 UV, IR 스펙트럼을 살펴보며 이들의 pH값을 제시한댜. 즉 5'-XMP 및 5'GMP등과 같은 nucleotide류는 모두 phosphomonoester group을 가지고 있는 제 1인산이 약 1, 제 2인산이 6정도인 2개씩의 pk값을 가지고 있으며 이외에도 pyrimidine핵에 OH기와 $NH_2가$ 있어서 용액의 pH에 따라 여러가지 형태의 이온형을 가질 수 있다.(중략)

• Proceedings of the Korean Information Science Society Conference
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• 2002.10d
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• pp.367-369
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• 2002

DNA 칩에서 사용되는 프로브를 가장 효과적으로 설계하기 위해서는 상보결합을 위한 1차구조뿐만이 아니라 열역학적인 움직임과 함께 2차구조가 고려되어야만 한다. 그러나 핵산의 기능에 큰 영향을 미치는 2차구조에 대한 연구는 일찍부터 진행되어 왔지만, 상대적으로 DNA에 대한 연구는 크게 미흡한 것이 현실이다. 이에 우리는 유전자 알고리즘을 이용한 핵산의 이차구조 예측을 통해서 보다 효과적인 프로브의 설계를 위한 방법을 고안했다.

• Journal of the Korean Chemical Society
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• v.10 no.3
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• pp.129-132
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• 1966

It is known that almost all cyclohexane molecules are chair form in liquid state. Therefore, only chair form is considered in formulating the partition function for liquid cyclohexane, according to the Transient State Theory of Significant Liquid Structure proposed by Pak, Ahn and Chang. The thermodynamic quantities such as molar volume, vapor pressure, entropy of vaporization and compressibility of the liquid are calculated. The results are in good agreement with experimental values.