• 제목/요약/키워드: 해양환경시료

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Alexandrium pacificum(Group IV) isolated from Jangmok Bay, Korea: Morphology, phylogeny, and effects of temperature, salinity, and nutrient levels on growth (장목만에서 분리한 유독 와편모조류 Alexandrium pacificum(Group IV): 형태, 계통 분류와 온도, 염분 및 영양염 농도 차이에 따른 성장 변화)

  • Kyong Ha Han;Joo Yeon Youn;Kyeong Yoon Kwak;Zhun Li;Wonchoel Lee;Hyeon Ho Shin
    • Korean Journal of Environmental Biology
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    • v.40 no.3
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    • pp.290-300
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    • 2022
  • A strain of Alexandrium species was established by isolating cells from Jangmok Bay, Korea. Its morphology and molecular phylogeny based on LSU rRNA gene sequences were examined. In addition, growth responses of this Alexandrium species to changes in temperature, salinity, and nutrient concentrations were investigated. This Alexandrium species from Jangmok Bay had a ventral pore on the 1', which was morphologically consistent with previously described Alexandrium tamarense and A. catenella. Phylogenetic analyses revealed that this isolate was assigned to A. pacificum (Group IV) within A. tamarense species complex. In growth experiments, relatively high growth rates and cell densities of A. pacificum (Group IV) were observed at 15℃ and 20℃. This species also grew under a wide range of salinity. This indicates that this Korean isolate of A. pacificum (Group IV) is a stenothermic and euryhaline species. In growth responses to changes in nutrient levels, enhanced growth rates and cell densities of A. pacificum(Group IV) were observed with additions of nitrate and phosphate. In particular, rapid uptakes of phosphate by A. pacificum (Group IV) were observed in experimental treatments, indicating that the increase in phosphate concentration could stimulate the growth of A. pacificum(Group IV).

Sedimentary Environments and Heavy Metallic Pollution at Shihwa Lake (시화호의 퇴적환경과 중금속오염)

  • Hyun, Sang-Min;Chun, Jong-Hwa;Yi, Hi-Il
    • The Sea:JOURNAL OF THE KOREAN SOCIETY OF OCEANOGRAPHY
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    • v.4 no.3
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    • pp.198-207
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    • 1999
  • Five core sediments acquired from the Lake Shihwa are analyzed for variations of sedimentary environment and heavy metal pollution after the Shihwa seawall construction. The depositional environment of the study area is divided into anoxic, oxic and mixed suboxic conditions based on the C/N ratio and C/S ratios of organic matters. Controlling factors for redox condition are the water depth and the difference in industrial effluents supply. Correlations among geochemical elements (Mn, U, Mo) show a distinctive difference and thus can be used as an indicator of redox condition. The content of Al, Ti are dependent on the sediment characteristics, and the contents of heavy metals (Cr, Ni, Cu, Zn, and Pb) indicate heavy metal pollution. The concentrations of heavy metals are higher near Shiswa-Banwol industrial complexies than the central part of Lake Shihwa. Especially, the accumulation of the heavy metal at the surface sediments near Shihwa-Banwol industrial complex are two to eight times higher than in the center of Lake Shihwa.

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Residue of benomyl in the coastal environment on the Cheju island (제주도(濟州道) 연안(沿岸) 해양(海洋) 중(中) benomyl의 잔류(殘留))

  • Kim, Jung-Ho;Suh, Seung-Kyo;Oh, Youn-Keun
    • The Korean Journal of Pesticide Science
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    • v.3 no.1
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    • pp.51-56
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    • 1999
  • For the determination of coastal environmental contamination level of benomyl, benzimidazole pesticide, the residue of benomyl in various environmental samples on the Cheju island was monitored in May, August and October 1996, respectively. The residue of benomyl was determined as carbendazim because benomyl was converted to carbendazim (methyl 2- benzimidazolecarbamate) in the environment. The qualified limit detection of benomyl was $0.2{\mu}g/L$ in ocean water and $0.4{\mu}g/kg$ in the solid such as sediment and sea organism by HPLC with UV detector. Benomyl was not detected in any water and sediment. Moreover benomyl was neither detected in seaweed cava(Ecklonia Cava), agar(Gelidium amansii), turban sell(Batillus cornutus) and sea urchin(Anthocidaris Crassispina). Above date suggest that the benomyl used in the Cheju island is not the major source of coastal contamination.

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Application of Environmental DNA (eDNA) for Marine Biodiversity Analysis (해양생물 다양성 연구를 위한 환경유전자(eDNA)의 적용)

  • Soyun Choi;Seung Jae Lee;Eunkyung Choi;Euna Jo;Jinmu Kim;Minjoo Cho;Jangyeon Kim;Sooyeon Kwon;Hyun Park
    • Journal of Marine Life Science
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    • v.8 no.2
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    • pp.93-103
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    • 2023
  • eDNA, an abbreviation for environmental DNA, means DNA derived from organisms inhabiting in a specific environment. The utilization of eDNA extracted from environmental samples allows for efficient and accurate monitoring of organisms inhabiting the respective environment. Specifically, eDNA obtained from seawater samples can be used to analyze marine biodiversity. After collecting seawater samples and extracting eDNA, metagenome analysis enables the taxonomic and diversity analysis among marine organisms inhabiting the sampled area. This review proposed an overall process of marine biodiversity analysis by utilizing eDNA from seawater. Currently, the application of eDNA for analyzing marine biodiversity in domestic setting is not yet widespread. This review can contribute to establishment of marine eDNA research methods in Korea, providing valuable assistance in standardizing the use of eDNA in marine biodiversity studies.

Ecological Effects of Slag Extracts on the Initial Life Cycle of the Rotifer Brachionus plicatilis and Benthic Copepod Tigriopus japonicus (윤충류 Brachinus plicatilis와 저서성 요각류 Tigriopus japonicus의 초기생활사에 미치는 슬래그 추출액의 생태 영향)

  • Yoon, Sung-Jin
    • Journal of the Korean Society of Marine Environment & Safety
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    • v.22 no.5
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    • pp.490-499
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    • 2016
  • In this study, the marine ecological impacts of dephosphorized slag and steel slag on the initial life cycle of the rotifer Brachionus plicatilis and benthic copepod Tigriopus japonicus (in marine trophic structure as a first consumer) exposure to slag extracts have been considered using a marine ecotoxicological assessment. In the results of a screen test on slag extracts, the pH of an undiluted solution was measured to have high alkalinity (pH 8.89-12.16), but a toxic reaction to this undiluted solution before and after aging was divided according to test species. For non-aged slag, the toxic effect ($LC_{50}$) of neonate on B. plicatilis was seen to be severe with dephosphorized slag (20.8 %) than steel slag (63.8 %) with under pH-uncontrolled conditions. The toxic values of dephosphorized and steel slag were estimated to be 35.3 % and 36.0%, respectively, for nauplius with T. japonicus. However, the toxicity of slag extracts before and after aging were different for T. japonicus than for B. plicatilis based on the characteristics of the test materials, with pH-controlled conditions. In conclusion, the results of this study suggest that slag can be relatively stable after aging and may not be likely to influence marine environments, even given repetitive extracting under pH-uncontrolled conditions. This study confirms that a marine ecotoxicological assessment method applied to mechanically activated samples can give an idea of the resistance a marine environment has against the introduction of hazardous materials due to precipitation and weathering.

Improvement of Oxygen Isotope Analysis in Seawater samples with Stable Isotope Mass Spectrometer (질량분석기를 이용한 해수 중 산소안정동위원소 분석법의 개선)

  • Park, Mi-Kyung;Kang, Dong-Jin;Kim, Kyung-Ryul
    • The Sea:JOURNAL OF THE KOREAN SOCIETY OF OCEANOGRAPHY
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    • v.13 no.4
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    • pp.348-353
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    • 2008
  • Oxygen isotope has not been used actively in water mass studies because of difficulties on the analysis though it has advantages as a water mass tracer. The most popular method to analysis the oxygen isotope ratio in water samples is equilibration method: isotopic equilibrium of water with $CO_2$ at constant temperature. The precision of oxygen isotope analysis using commercial automatic $H_2O/CO_2$ equilibrator is ${\pm}0.1%o$. This value is not sufficient for studies in open ocean. The object of this study is to improve the analytical precision enough to apply open ocean studies by modification of the instrument. When sample gas is transferred by the pressure difference, the fractionation which is preferential transportation of light isotope can be occurred since the long transportation path between the equilibrator and mass spectrometer. And the The biggest source of error during the analysis is long distance and large volume of the pathway of sample gas between. Therefore, liquid nitrogen trap and high vacuum system are introduced to the system. The precisions of 14 time analysis of same seawater sample are ${\pm}0.081%o$ and ${\pm}0.021%o$ by built-in system and by modified system in this study, respectively.

A Geo-statistical Assessment of Heavy Metal Pollution in the Soil Around a Ship Building Yard in Busan, Korea (통계지표를 활용한 부산지역 조선소 주변 토양 내 중금속 오염조사 연구)

  • Choi, Jung-Sik;Jeon, Soo kyung
    • Journal of the Korean Society of Marine Environment & Safety
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    • v.24 no.7
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    • pp.907-915
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    • 2018
  • With the increase of metal usage in various industries, metal pollution and ecological toxicity in the environmental system have become a significant concern. A geo-statistical index has been widely used to determine contamination level with normalization through a background value. In this study, geo-statistical indexes such as an enrichment factor, accumulation index, and potential ecological risk index were used to assess metal pollution in soil at locations associated with shipbuilding manufacturing industries. Metal contamination, especially of Cu and Pb, was observed in some samples located closer to manufacturing sites. Enrichment factor and accumulation (IGEO) values were indicative of concerning levels of soil contamination in specific samples, and the soil contamination could be induced by anthropogenic sources. In further study, after more detailed sampling for soil and potential pollution sources, high interpretation techniques such as Pb isotope analysis and X-ray analysis will be needed to investigate source identification.

Physico-Chemical Properties and Methane Production Rates for Busan Harbor Sediments (부산항만 퇴적물의 성분분석 및 메탄발생량 산정 연구)

  • Choi, boram;Lee, taeyoon
    • Journal of the Korean GEO-environmental Society
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    • v.12 no.5
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    • pp.37-42
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    • 2011
  • The main objective of this study was to evaluate the current condition of harbor sediments and to estimate biochemical methane potentials from the harbor sediments. Sediment samples were collected from 10 different sampling sites. Ignition loss, elemental analyses, X-ray diffraction(XRD), X-ray fluorescence(XRF) tests were conducted to determine characteristics of the sediment. All sediments had similar elemental compositions and ignition loss were 8~10%. From the conventional BMP tests for 5 samples, cumulative methane production ranged from 11.9~15.5mL methane/(g of volatile solids), which were significantly lower than that for foods and paper. However, methane production rates for sediments were 5 to 20 times faster than those for foods and paper.

해양퇴적물의 생물학적 오염평가를 위한 시험생물 개발에 관한 연구

  • 이정석;이규태;이종현;박경수;박승윤
    • Proceedings of the Korean Society of Fisheries Technology Conference
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    • 2003.05a
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    • pp.144-145
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    • 2003
  • 퇴적물 오염 평가는 크게 화학적 평가, 독성학적 평가 그리고 생태학적인 평가로 나눌 수 있다 (Long et al. 1995) 최근 국내에서도 오염된 연안의 저서환경에 대한 과학적이고 체계적인 평가 방법에 대한 관심이 크게 증대하고 있다. 그러나 아직까지 퇴적물을 비롯한 환경시료에 대한 독성학적인 오염 평가에 대해서는 많은 연구가 이루어지지 못한 실정이다. (중략)

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