• Proceedings of the Plant Resources Society of Korea Conference
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• 2002.11b
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• pp.67-67
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• 2002

아열대성 식물 4종 (문주란, Crinum asiaticum var. japonicum; 박달목서, Osmanthus insularis; 죽절초, Chloranthus glaber; 파초일엽, Asplenium antiquum)을 대상으로 자연 환경요인의 변화에 의한 항산화 효소 (superoxide dismutase, peroxidase, catalase, ascorbate peroxidase)의 활성과 isoenzyme 패턴의 변화를 전기영동으로 조사하였다. 그 결과, peroxidase의 활성과 isoenzyme 패턴이 식물종이나 환경조건에 따라 가장 다양하게 나타났다. Peroxidase는 4종 모두에서 여름철보다 겨울철에 활성이 높았고 문주란, 박달목서, 파초일엽에서는 겨울철에 특이적으로 발현되는 isoenzyme들도 관찰할 수 있었다. Catalase는 문주란, 박달목서, 파초일엽에서 검출되었다. 문주란 잎에서는 겨울철에 비해 여름철에 다소 높은 활성을 보였으며, 박달목서와 파초일엽에서는 겨울철에 높은 활성을 나타내었다. 그리고 문주란과 박달목서에서는 겨울철에 새벽이나 밤보다 낮시간에 높은 활성을 보였는데 파초일엽에서는 낮시간의 catalase 활성이 낮았다. Superoxide dismutase는 문주란, 박달목서, 파초일엽에서 검출되었으며, 특히 박달목서에서는 겨울철에 높은 활성을 보였다. Ascorbate peroxidase는 문주란과 파초일엽에서 관찰되었으나 계절적으로 큰 차이가 없었으며, 겨울철에는 isoenzyme 패턴의 일주기적 변화가 관찰되었다. 이상의 결과, 종별로는 문주란, 파초일엽에서 4종의 항산화효소가 모두 검출되었고, 박달목서에서는 ascorbate peroxidase가, 죽절초에서는 peroxidase를 제외한 모든 항산화 효소가 검출되지 않았다. 식물종에 따라 또는 환경요인의 변화에 따라 항산화효소의 활성 또는 isoenzyme 패턴의 차이를 보이고 있지만 항산화효소의 계절적 그리고 일주기적 변화가 관찰되어, 본 연구에서 조사된 4종의 아열대성 식물이 자연환경 조건 하에서도 산화적 스트레스에 처하고 있는 것으로 보인다.

• Korean Journal of Plant Tissue Culture
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• v.22 no.2
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• pp.71-76
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• 1995

The effects of light on the activities of several antioxidative enzymes, catalase (CAT), superoxide dismutase(SOD), ascorbate oxidase(AO), and peroxidase(POD) were examined in the hairy root cultures of Phytolacca esculenta van Houtte induced by Agrobacterium tumefaciens $A_4$T. Activities of CAT, SOD, and AO were significantly decreased with incresing light intensity (500-2,000 lx). The activity of AO under high light condition (2,000 lx)was decreased by 92% compared to the dark condition. The activities of glutathoine peroxidase (GPO), ascorbate peroxidase (APO) and general POD were increased under lower light intensify below 500 lx. The activity of GPO under 2,000 lx was decreased by 85% compared to the dark condition. The activities of antioxidative enzymes were more decreased in blue light (400-500nm). The activities of antioxidative enzymes in blue light intensity were increased in lower light intensity below 30 lx, but decreased 21-70% under 200 lx. The activity of AO was decreased by 70% under 200 lx with increasing blue light intensity. Our results suggest that the activities of antioxidative enzymes in hairy roots might be inhibited by endogenous oxidants generated under the high blue light conditions.

• Journal of Life Science
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• v.12 no.3
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• pp.349-356
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• 2002

In order to observe the bioactivity of ovariectomized rats, nonovariertoized (sham) group, ovariectomized (Ovx) group, ovariectomized ginseng total saponin (GTS)-treated (Ovx+ GTS) group and ovariectomized ginseng water extract (GW)-treated (Ovx+CW) group were made. We measured AST (L-aspartate aminotransferase) and ALT (L-alanin aminotransferase) in sera, and MDA (malondialdehyde:lipid peroxidation), SOD (superoxide dismutase), catalase, total-glutathione (GSH + GSSG) and GPx (glutathione peroxidase) in liver tissue total homogenates of rat. AST activity of serum in Ovx group was 2.11 times increased, but ALT activity was not changed compared to Sham group. In AST activity, they tend to decrease significantly in each substance such as GTS and GW administered group. Lipidperoxides of each fraction in Ovx group were highly increased compared to Sham group. Extracts of ginseng-treated group markedly inhibited lipid peroxidation by 62% ∼72%. And as the result of the measurements of SOD, catalase, total-glutathione and GPx which are antioxidant enzyme, antioxidant enzymes in Ovx group much lower than in Sham group. But they were significantly increased in each substance such as GTS and GW, administered group. Based on the results, it is supposed that more produced free radicals decreased antioxidant enzyme. And it is also thought that extracts of ginseng can inhibit aging by reducing antioxidant enzyme.

• Korean Journal of Environment and Ecology
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• v.16 no.1
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• pp.46-54
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• 2002

This study was conducted to diagnose the health of street trees with physiological characters, and to figure out the relation of physiological characters and cambial electrical resistances. Ginkgo biloba, Prunes serrulata and Salix koreensis were chosen in the Cheonan City. Soils under trees were collected to analyze dehydrogenase activities, and chlorophyll content, nitrate reductase and superoxide dismutase activities were analyzed from leaves sampled at the edge of crown in July. Cambial electrical resistances were measured in May, July and September, Soils with low dehydrogenase activity reflected the level of pollution. Chlorophyll content was the lowest in the leaves of P. serrulata at the Dongseo-street. Nitrate reductase activity of Ginkgo biloba was higher than P. serruluta and Satix koreensis. Nitrate reductase activity showed higher activity in the city than control(Independence Hall and Yonam College), but superoxide dismutase activity in the city lower than control. P. serruzatu in the Dongseo-street that cambial electrical resistance increase continuously during the growing season, showed the loss of vitality Cambial electrical resistance was negatively or positively correlated with nitrate reductase($r^2$=-0.566) and superoxide dismutase activity($r^2$=0.579). It was concluded that cambial electrical resistance might be suitable for diagnosing the tree health.

• Korean Journal of Weed Science
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• v.17 no.4
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• pp.431-438
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• 1997

The influence of deficient(7${\mu}M$) and suffcient(1000${\mu}M$) magnesium(Mg) supply on the content of mg, chlorophyll, protein and the activity of superoxide dismutase(SOD), scavengers of superoxide radical($O_2$), and ascorbate peroxidase(AP), $H_2O_2$scavenging enzyme, and glutathione redutase(GR) were studied in pumpkin(Cudurbita moscata $D_{UCHESNE}$) plants over a 11-day period. Over the 11 days Period of growth in nutrient solution with sufficient and deficient Mg supply, the contents of Mg, chlorophyll protein and the activities of the antioxidative enzymes remained more or less constant in Mg-sufficient leaves. In Mg-deficient leaves, the contents of Mg, chlorophyll and protein was seriously decreased with time, however the activities of SOD, AP and GR highly enhanced compared to those of Mg-sufficient. The results indicated the stimulative effect of Mg deficiency on toxic oxygen species and scavenging enzymes in plants.

• Journal of Korean Ophthalmic Optics Society
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• v.20 no.1
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• pp.75-81
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• 2015

Purpose: The present study was conducted to investigate whether the changes in structure and activity of antioxidative enzymes, superoxide dismutase(SOD) and catalase(CAT) present in the eyes appeared when they were repeatedly exposed to UV-A, and reveal the correlation of these changes. Methods: Each enzyme solution was prepared from the standardized SOD and CAT, and repeatedly exposed to UV-A of 365 min under the condition of 30 minutes, 1 hour and 2 hours a day over 1, 2, 3, 4 and 5 days. Structural denaturation of SOD and CAT induced by repeat UV-A irradiation was confirmed by the electrophoretic analysis, and their enzyme activity was determined by the colorimetric assay using the proper assay kit. Results: SOD exposed repeatedly to UV-A showed the polymerization pattern through the electrophoretic analysis when it was repeatedly exposed under the condition of at least 1 hour a day however, the change of its activity was found to be less than 12%. On the other hand, CAT repeatedly exposed to UV-A showed reduced size of the electrophoretic band which indicated a structure denaturation and its activity was significantly decreased. In the case of that the repeat exposure time was longer, CAT activity was completely lost even though some enzyme band was shown in the electrphoretic analysis. Conclusions: From these results, it was revealed that the degree and pattern in structural denaturation of antioxidative enzymes differently appeared according to the type of enzyme, and the degree of structural denaturation was not always consistent with the reduction in enzyme activity.

• Korean Journal of Environmental Biology
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• v.24 no.2 s.62
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• pp.119-125
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• 2006

Present study aims to study antioxidant enzyme activity due to turbidity change in various tissues of Carassius auratus. As for the changes of antioxidant enzyme activity in tissues of C. auratus pursuant to the raising period under 50, 100, and 150 NTU with turbid water levels, there was no great difference between 50 NTU and 100 NTU compared to a control (0 NTU), however, it demonstrated a relatively noticeable difference at 150 NTU high turbid water level. When considering the antioxidant capacity in tissues of C. auratus in terms of DPPH free radical scavenging activity, there was shown a high activity in gill and liver tissues, therefore, it is thought that there appears a non-enzymatic antioxidant reaction when C. auratus is reared under the condition of highly turbid water. As for the enzymatic antioxidant reaction of antioxidant enzyme activity got increased as turbid water level went higher in order of 50, 100, 150 NTU, and the activities of superoxide dismutase (SOD), catalase (CAT), and glutathione-s-transperase (GST), increased in all tissues except for an integument, up to 20th day when it was started to be reared, and they showed a gradual decrease as time passed by. However, since the activity of glutathione reductase (GR) and glutathione peroxidase (GPX) is very low in almost all tissues, it is thought that the role of those enzymes would be quite ignorable in the course of antioxidant process.

• Korean Journal of Soil Science and Fertilizer
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• v.32 no.2
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• pp.164-170
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• 1999

This experiment was carried out to elucidate change of antioxidant enzymes activities subjected to water stresses in soybean plant. In this study, we measured the activities of ascorbate peroxidase(APDX), monodehydroascorbate reductase(MDHAR), dehydroascorbate reductase (DHAR), glutathione reductase(GR) subjected to drought or flooding stresses for 4days and following recovery for 3days. Leaves of two soybean lines subjected to drought or flooding showed premature senescence as evidenced by the decrease in water content and total soluble protein content but those of soybean leaves was increased when stresses were recovered for 3days. The activities of APDX and GR subjected to drought or flooding were the decrease but those of enzymes were recovered when water stress was recovered. The activities of MDHAR with drought or flooding were on the decrease, whereas those of DHAR were increased, respectively. Antioxidant contents decreased continually subjected to drought or flooding but it recovered after 3 days subjected to water stresses.

• Korean Journal of Environmental Biology
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• v.20 no.2
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• pp.173-179
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• 2002

N-nitrosoethylurea (NEU) -induced changes of lipid peroxide content, aldehyde metabolic enzyme activities and antioxidant enzyme activities were examined in cultured rat liver cell. Aldehyde metabolic enzymes tested in this investigation were alcohol dehydrogenase and aldehyde dehydrogenase. Several antioxidant enzymes tested were glutathione transferase, superoxide dismutase, glutathione reductase and catalase. When the cell was exposed with various concentrations of NEU, lipid peroxide content increased about 2.5 fold with 6.25 mM NEU. Maximun 2.3 times higher alcohol dehydrogenase activity was found after NEU treatment. About 2 times higher aldehyde dehydrogenase activity could also be observed. Only slight increases of glutathione transferase and catalase activities occurred with NEU treatment. In addition mnximun 1.5 times higher superoxide dismutase activities and 3 times higher glutathione reductase activities were found after NEU treatment. Therefore, it is likely that the increases of superoxide dismutase and glutathione reductase could contribute in a antioxidative process against NEU toxicity.

• Food Science and Preservation
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• v.31 no.3
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• pp.444-451
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• 2024

This study aimed to investigate the characteristics of protein hydrolysates derived from defatted egg yolk using various proteolytic enzymes and compare the antioxidant activity of the resulting hydrolysates. The defatted egg yolk powder was subjected to enzymatic hydrolysis using four different proteases (alcalase, bromelain, flavourzyme and neutrase), and the resulting hydrolysates were evaluated for their antioxidant properties. Through analysis of available amino group contents and sodium dodecyl sulfate-polyacrylamide gel electrophoresis, it was observed that the defatted egg yolk powder treated with alcalase, flavourzyme, and neutrase for 12 h exhibited a high degree of hydrolysis value. Based on the RC₅₀ values obtained from two different antioxidant analyses, all hydrolysates showed comparable antioxidant activity, except for the alcalase hydrolysate, which demonstrated notably higher scavenging activity against hydrogen peroxide than the other hydrolysates. These findings suggest the potential of protein hydrolysates from defatted egg yolk, a by-product of lecithin extraction, as natural antioxidants.