Food Science and Preservation (한국식품저장유통학회지)

The Korean Society of Food Preservation (한국식품저장유통학회)
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Enzymatic preparation and antioxidant activities of protein hydrolysates from defatted egg yolk

탈지난황을 이용한 단백가수분해물 제조 및 항산화 활성 평가

  • Go-Eun Ko (Department of Food Science and Technology, Keimyung University) ;
  • Na-Yeong Kwak (Department of Food Science and Technology, Keimyung University) ;
  • Ha-Eun Nam (Department of Food Science and Technology, Keimyung University) ;
  • Su-Jin Seo (Department of Food Science and Technology, Keimyung University) ;
  • Syng-Ook Lee (Department of Food Science and Technology, Keimyung University)
  • 고고은 (계명대학교 식품가공학과) ;
  • 곽나영 (계명대학교 식품가공학과) ;
  • 남하은 (계명대학교 식품가공학과) ;
  • 서수진 (계명대학교 식품가공학과) ;
  • 이승욱 (계명대학교 식품가공학과)
  • Received : 2024.04.09
  • Accepted : 2024.05.24
  • Published : 2024.06.30
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Abstract

This study aimed to investigate the characteristics of protein hydrolysates derived from defatted egg yolk using various proteolytic enzymes and compare the antioxidant activity of the resulting hydrolysates. The defatted egg yolk powder was subjected to enzymatic hydrolysis using four different proteases (alcalase, bromelain, flavourzyme and neutrase), and the resulting hydrolysates were evaluated for their antioxidant properties. Through analysis of available amino group contents and sodium dodecyl sulfate-polyacrylamide gel electrophoresis, it was observed that the defatted egg yolk powder treated with alcalase, flavourzyme, and neutrase for 12 h exhibited a high degree of hydrolysis value. Based on the RC50 values obtained from two different antioxidant analyses, all hydrolysates showed comparable antioxidant activity, except for the alcalase hydrolysate, which demonstrated notably higher scavenging activity against hydrogen peroxide than the other hydrolysates. These findings suggest the potential of protein hydrolysates from defatted egg yolk, a by-product of lecithin extraction, as natural antioxidants.

본 연구에서는 탈지난황을 활용한 항산화 소재 개발 가능성을 탐색하기 위해 탈지난황 단백가수분해물을 제조하고 항산화 활성을 평가하였다. 4종의 단백질 가수분해 효소(alcalase, bromelain, flavourzyme, neutrase)를 처리한 후 시간에 따른 각 효소별 가수분해도를 확인한 결과, 효소 처리군의 가수분해도가 비처리군에 비하여 전반적으로 높았으며, 특히 DEYA가 가장 높은 가수분해도를 보였다. 각 효소와 12시간 반응시켜 제조한 효소별 단백가수분해물의 ROS 소거 활성을 평가하기 위해 ABTS radical 및 H2O2 소거 활성 실험을 진행한 결과, DEYA가 다른 가수분해물에 비해 유의적으로 높은 항산화 활성을 보이는 것으로 확인되었다. 이상의 실험을 기반으로 효소별 단백가수분해물 중 가장 높은 가수분해도와 항산화 활성을 보였던 DEYA를 상온에 보관하며 항산화 안정성 평가를 진행한 결과, DEYA는 L-ascorbic acid와 녹차 추출물 대비 월등히 우수한 안정성을 보였으며, 28일 차까지도 90% 이상의 항산화 활성을 유지하였다. 또한, DEYA는 난백 단백가수분해물, 갈색 거저리 유충 그리고 탈지대두와의 ABTS radical 소거 활성 비교 실험을 진행한 결과에서도 유사하거나 상대적으로 우수한 활성을 보였다. 최종적으로 본 연구에서는 산업적으로 이용되고 있는 lecithin 추출 공정과 효소적 가수분해를 통해 제조한 탈지 난황 단백가수분해물의 우수한 항산화 활성을 확인할 수 있었다. 이는 향후 항산화 기능성을 가진 기능성 식품과 식품첨가물 개발을 위한 기초 연구 자료로서 활용될 수 있을 것으로 기대된다.

Keywords

Acknowledgement

This research was supported by Support Program for University Development Program through the National Research Foundation of Korea (NRF) funded by the Ministry of Education.

References

  1. Bouayed J, Bohn T. Exogenous antioxidants-double-edged swords in cellular redox state. Oxid Med Cell Longevity 3, 228-237 (2010)
  2. Castro RJS de, Sato HH. Synergistic actions of proteolytic enzymes for production of soy protein hydrolysates with antioxidant activities: An approach based on enzymes specificities. Biocatal Agric Biotechnol 4, 694-702 (2015)
  3. Cho DY, Jo K, Cho SY, Kim JM, Lim K, Suh HJ, Oh S. Antioxidant effect and functional properties of hydrolysates derived from egg-white protein. Korean J Food Sci Anim Resour, 34, 362-371 (2014)
  4. Cho HR, Lee YJ, Hong JE, Lee SO. Enzymatic preparation and antioxidant activities of protein hydrolysates from Gryllus bimaculatus. Korean J Food Sci Technol, 51, 473-479 (2019)
  5. Cho YJ, Ju IS, Chun SS, An BJ, Kim JH, Kim MU, Kwon OJ. Screening of biological activities of extracts from Rhododendron mucronulatum Turcz. flowers. J Korean Soc Food Sci Nutr, 37, 276-281 (2008)
  6. Ha YJ, Ji JG, Yoo SK. Antioxidant activity of ogae egg white protein hydrolysates using commercial protease. Korean J Oil Fat Chem, 34, 631-642 (2017)
  7. Han JK, Oh MH, Nam JO, Ji KB, Sim IS, Park KT. Efficacy of supplemental lecithin-free egg yolk at different dietary calcium levels on growth performance and Ca absorption of laying hens. KSBB J, 30, 58-62 (2015)
  8. Hong JK. A Study on skin aging caused by free-radical and on efficacy of antioxidant vitamins. Kor J Aesthet Cosmetol, 7, 51-62 (2009)
  9. Jang HY, Park CE, Lee SO. Comparison of antioxidant capacity of protein hydrolysates from 4 different edible insects. Korean J Food Sci Technol, 51, 480-485 (2019)
  10. Jeong HR, Kim JM, Kang JY, Moon JH, Kim MJ, Lee HL, Go MJ, Gu SY, Kim HJ, Heo HJ. Antioxidant activity and neuroprotective effect of ethyl acetate fraction of Lonicera japonica on H2O 2-induced oxidative stress. Asian J Beauty Cosmetol, 28, 968-979 (2021)
  11. Jeong YH, Jeong GH, Kim TH. Radical scavenging and α-glucosidase inhibitory effects of Mongolian Iris bungei extract. Asian J Beauty Cosmetol, 24, 879-884 (2017)
  12. Kim JI, Kim MJ, Jo HG, Jeong DE, Park HJ, Cho YJ. Anti-oxidation, anti-inflammation, anti-wrinkle, and pore-tightening effects of phenolic compounds from Aeonium sedifolium leaves. Korean J Food Preserv, 30, 347-357 (2023)
  13. Lee HH, Moon YS. Assessment of the important factors influencing consistent and accurate ABTS assay. J Korean Soc Food Sci Nutr, 48, 390-393 (2019)
  14. Lee KY, Han CG, Zhoh CK. The constraint for oxidation of ascorbic acid. J Soc Cosmet Sci Korea, 25, 67-86 (1999)
  15. Lee KY, Han CY, Pyo MJ, Choi SG. Effect of red paprika powder on quality and oxidative stability of mayonnaise prepared with perilla oil. Korean J Food Preserv, 29, 932-942 (2022)
  16. Na EJ, Jang HH, Kim GR. Review of recent studies and research analysis for anti-oxidant and anti-aging materials. Asian J Beauty Cosmetol, 14, 481-491 (2016)
  17. Nain CW, Berdal G, Thao PTP, Mignolet E, Buchet M, Page M, Larondelle Y. Green tea extract enhances the oxidative stability of DHA-rich oil. Antioxidants, 10, 982-995 (2021)
  18. Park GH, Lee JM, Lim NY, Lee SO. Enzymatic preparation and antioxidant activities of protein hydrolysates derived from tuna byproducts. Korean J Food Preserv, 30, 885-895 (2023)
  19. Sarantidi E, Ainatzoglou A, Papadimitriou C, Stamoula E, Maghiorou K, Miflidi A, Trichopoulou A, Mountzouris KC, Anagnostopoulos AK. Egg white and yolk protein atlas: New protein insights of a global landmark food. Foods, 12, 3470-3488 (2023)
  20. Seo YB. Extraction and purification of egg yolk lipid. MS Thesis, Inje University, Korea, p 5-10 (2012)
  21. Wang S, Wang Y, Ma J, Ding Y, Zhang S. Phosvitin plays a critical role in the immunity of zebrafish embryos via acting as a pattern recognition receptor and an antimicrobial effector. J Biol Chem, 286, 22653-22664 (2011)
  22. Wouters AGB, Rombouts I, Fierens E, Brijs K, Delcour JA. Relevance of the functional properties of enzymatic plant protein hydrolysates in food systems. Compr Rev Food Sci Food Saf, 15, 786-800 (2016)
  23. Yang EJ, Lee YE, Moon HK. Nutritional roles and health effects of eggs. J Nutr Health, 47, 385-393 (2014)
  24. Yeo SG, Ahn CW, Lee YW, Lee TG, Park YH, Kim SB. Antioxidative effect of tea extracts from green tea, Oolong tea and black tea. J Korean Soc Food Sci Nutr, 24, 299-304 (1995)
  25. Yoon SR, So BR, Park JM, Ryu JA. Changes in antioxidant activity and vitamin B2 content of Aster glehni based on blanching time. Korean J Food Preserv, 29, 1139-1149 (2022)
  26. Yu BJ, Chang MH, Jeong IH. Antioxygenic effects of browning reaction product obtained from L-ascorbic acid solution. Korean J Food Sci Technol, 23, 622-626 (1991)
  27. Yu MH, Lee HS, Cho HR, Lee SO. Enzymatic preparation and antioxidant activities of protein hydrolysates from Tenebrio molitor Larvae (mealworm). J Korean Soc Food Sic Nutr, 46, 435-441 (2017)
  28. Zhao F, Li R, Liu Y, Chen H. Perspectives on lecithin from egg yolk: extraction, physicochemical properties, modification, and applications. Front Nutr, 9, 1082671 (2023)