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Effects of N-nitrosoethylurea on the Activities of Antioxidant Enzymes from Rat Liver Cell  

이미영 (순천향대학교 생명과학부)
Publication Information
Korean Journal of Environmental Biology / v.20, no.2, 2002 , pp. 173-179 More about this Journal
Abstract
N-nitrosoethylurea (NEU) -induced changes of lipid peroxide content, aldehyde metabolic enzyme activities and antioxidant enzyme activities were examined in cultured rat liver cell. Aldehyde metabolic enzymes tested in this investigation were alcohol dehydrogenase and aldehyde dehydrogenase. Several antioxidant enzymes tested were glutathione transferase, superoxide dismutase, glutathione reductase and catalase. When the cell was exposed with various concentrations of NEU, lipid peroxide content increased about 2.5 fold with 6.25 mM NEU. Maximun 2.3 times higher alcohol dehydrogenase activity was found after NEU treatment. About 2 times higher aldehyde dehydrogenase activity could also be observed. Only slight increases of glutathione transferase and catalase activities occurred with NEU treatment. In addition mnximun 1.5 times higher superoxide dismutase activities and 3 times higher glutathione reductase activities were found after NEU treatment. Therefore, it is likely that the increases of superoxide dismutase and glutathione reductase could contribute in a antioxidative process against NEU toxicity.
Keywords
N-nitrosoethylurea; rat liver cell; antioxidant enzymes;
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  • Reference
1 Aldehyde dehydrogenase and their role in carcinogenesis /
[ Lindahl R ] / Crit. Rev. Biochem. Mol. Biol.   DOI   ScienceOn
2 Ki-ras mutations in spontaneous and chemically induced renal tumors of the rat /
[ Ohgaki H;P Kleihues;GC Hard ] / Mol. Carcinog.   DOI   ScienceOn
3 Changes in the pattern of aldehyde dehydrogenase activity in primary and metastatic adenocarcinomas of the human colon /
[ Marselos M;G Michalopouros ] / Cancer Letters   DOI   ScienceOn
4 Increase in class 2 aldehyde dehydrogenase expression by arachidonic acid in rat hepatoma cells /
[ Canuto RA;M Ferro;RA Salvo;AM Bassi;A Trombetta;M Maggiora;G Martinasso;R Lindahl;G Muzio ] / Biochem. J.   DOI   ScienceOn
5 The multistep nature of cancer development /
[ Farber E ] / Cancer Res.
6 studies of initiation and promotion of carcinogenesis by N-nitroso compounds /
[ Hasegawa R;M Futakuchi;Y Mizoguchi;T Yamaguchi;T Shirai;N Ito;W Lijinsky ] / Cancer Lett.   DOI   ScienceOn
7 Inhibition of cytosolic class 3 aldehyde dehydrogenase by antisense oligonucleotides in rat hepatoma cells /
[ Muzio G;RA Canuto;T Arombetta;M Maggiora ] / Chem. Biol. Interact.   DOI   ScienceOn
8 Human P-450 genes : evolution, regulation and possible role in carcinogenesis /
[ Nebert DW;AK Jaiswal;UA Meyer;FJ Gonzalez ] / Biochem. Soc. Trans.   DOI
9 Molecular dynamics simulation of class 3 aldehyde dehydrogenase /
[ Wymore T;HB Nicholas;J Hempel ] / Chem. Biol. Interact.
10 Aldehyde dehydrogenase. Maintaining critical active site geometry at motif 8 in the class 3 enzyme /
[ Hempel J;I Kuo;J Perozich;BC Wang;R Lindahl;H Nicholas ] / Eur. J. Biochem.   DOI   ScienceOn
11 the changes of enzymes activites during diethylnitrosamine-induced carcinogenesis of cultured rat liver cell /
[ Kim HB;YN Yum;MY Lee ] / J. Toxicol. Pub. Health
12 Genotoxic effects of 4-hydroxynonenal /
[ Eckl P;H Esterbauer ] / Adv. Biosci.
13 Activation of neu by missense point mutation in the transmembrane domain in schwannomas induced in C2H/HeNCr mice by transplcemental exposure to N-nitrosoethylurea /
[ Buzard GS;T Enomoto;LM Anderson;AO Perantoni;DE Devor;JM Rice ] / J. Cancer Res. Clin. Oncol.   DOI   ScienceOn
14 Modification of the genetic effect of N-nitrosoethylurea in inbred mice by para-aminobenzoic acid /
[ Malashenko AM;SV Vasileva ] / Genetika
15 Phenotypic and karyotypic changes induced in cultured rat hepatic epithelial cells that express the "oval" cell phenotype by expose to N-methyl-N'-nitro-N-nitrosoguanidine /
[ Tsao MS;JW Grisham;KG Nelson;JD Smith ] / Am. J. Pathol.
16 Hepatocyte expression of tumor associated aldehyde dehydrogenase (ALDH-3) and p21 Ras following diethylnitrosamine initiation and chronic exposure to di (2-ethylhexyl) phthalate /
[ Richmond RE;JH Carter;HW Carter;FB Daniel;AB Deangelo ] / Carcinogenesis   DOI   ScienceOn
17 Aldehyde dehydrogenase in 2-acetaminofluorene-induced rat hepatomas /
[ Lindahl R ] / Biochem. J.   DOI
18 Cellular response to oxidative stress: the Ah gene battery as a paradigm /
[ Nebert DW;DD Petersen;AJ Fornace ] / Environ. Health Perspect   DOI
19 Effect of light/dark regimen on N-nitrosoethylurea-induced transplacental carcinogenesis in rats /
[ Beniashvili DS;S benjamin;DA baturin;VN Anisimov ] / Cancer Lett.   DOI   ScienceOn
20 Identification of a characteristic cytosolic polypeptide of rat preneoplastic hepatocyte nodules as placenta glutathione-S-transferase /
[ Rushmore TH;E Faber ] / Biochim. Biophysic. Res. Com.   DOI   ScienceOn
21 N-nitrosodiethylamine initiation of carcinogenesis in japanese medaka (Oryzias latipes): hepatocellular proliferation, toxicity and neoplastic lesions resulting from short term, low level exposure /
[ Brown-Peterson NJ;RM Krol;Y Zhu;WE Hawkins ] / Toxicol. Sci.   DOI   ScienceOn
22 Synergistic effect of radiation and N-nitrosoethylurea in the induction of lymphoma in mice: cellular kinetics and carcinogenesis /
[ Seyama T;T Kajitani;A Inoh;K Yanagihara;K Kamiya;K Yokoro ] / Jpn. J. Cancer Res.
23 Glutathione-S-transferase, alcoholdehydrogenase and aldehyde reductase activities during diethylnitrosamine-carcinogenesis in rat liver /
[ Canuto RA;G Muzio;M Maggiora;ME Biocca;MU Dianzani ] / Cancer Letters   DOI
24 Aldehyde dehydrogenase activity in a rat hepatoma /
[ Feinstein Rn;EC Cameron ] / Biochem. Biophys. Res. Commun.   DOI   ScienceOn
25 The oxidation of α, β unsaturated aldehydic products of lipid peroxidation by rat liver aldehyde dehydrogenase /
[ Mitchell DY;DR Petersen ] / Toxicol. Appl. Pharmacol.   DOI   ScienceOn
26 Aldehyde dehydrogenase gene superfamily : the 2000 update /
[ Sophos NA;A Pappa;TL Ziegler;V Vasiliou ] / Chem. Biol. Interact.   DOI
27 Effect of pretreatment with beta-naphthoflavone on tumorigenesis by N-nitrosoethylurea in five mouse strains /
[ Anderson LM;AB Jones;RM Kovatch ] / Cancer Lett.   DOI   ScienceOn