• 한국생물공학회:학술대회논문집
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• 2005.10a
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• pp.707-710
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• 2005

The antimicrobial activities of extracts from some of natural products and bacteriocins produced from lactic acid bacteria such as L. mesenteroides, L. citreum and P. pentosaceus have been investigated with using disc diffusion assay, challenge method and minimum inhibitory concentration method. The bacteriocin substances produced from L. mesenteroides, L. citreum and P, pentosaceus have shown strong antimicrobial activities against E. coli, S. aureus, P. aerugionosa, and B. subtillis while the extracts from phenonip and sandal wood have shown strong antimicrobial activities only against C. albicans. The bacteriocins produced from L. mesenteroides, L. citreum and P. pentosaceus could be a promising preservatives for cosmetics, pharmaceutical products and foods in future.

• Food Science of Animal Resources
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• v.31 no.4
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• pp.609-616
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• 2011

In this study, a LCH1227 bacterial strain that possesses anti-listerial activity was isolated from fermented food and identified as Lactobacillus salivarius LCH1227 based on its morphological and biochemical properties, as well as its 16S rRNA gene sequences. Anti-listerial substance also inhibited the growth of various Gram-positive bacteria, such as vancomycinresistant Enterococcus faecalis, Streptococcus agalactiae, Bacillus cereus, Lactobacillus fermentum. The highest level of production of antimicrobial substances from L. salivarius LCH1227 occurred during the early stationary phase. The antilisterial activity was found to be stable over a broad range of pH values (2.0-12.0) and after heat treatment. However, it was inactivated by proteolytic enzymes, indicating its proteinaceous nature. The apparent molecular mass of the partially purified anti-listerial substance, as measured by Tricine-SDS-PAGE, was approximately 5 kDa.

• Journal of Life Science
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• v.27 no.10
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• pp.1161-1167
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• 2017

In order to find a new antimicrobial bacterium, we performed screening for antimicrobial activity of bacteria isolated from the eggs of a sea hare. The newly identified strain was designated as Phaeobacter inhibens KJ-2, based on the biochemical characterization and 16S rRNA gene sequence analysis. A colony of P. inhibens KJ-2 showed a circular and ruler-like smooth form at the edge, and a brown color. However, when maintained with a longer incubation time, its coloring was transformed into dark brown. From the result of SEM, P. inhibens KJ-2 is a bacillus which has a length of $0.8{\sim}1.0{\mu}m$ and a width of $0.4{\sim}0.6{\mu}m$. The optimal growth and antimicrobial activity were observed by shaking the culture for 24 hr at $20^{\circ}C$, which showed potent activity against pathogenic bacteria including Vibrio logei, Vibrio campbellii, Vibrio mimicus, Vibrio vulnificus, and Vibrio salmonicida. The antimicrobial activity was proportional to the amount of produced acylated homoserine lactones (AHLs). Therefore, we suggest that production of antimicrobial materials from P. inhibens KJ-2 is regulated by Quorum sensing (QS).

• Journal of Life Science
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• v.34 no.8
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• pp.540-547
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• 2024

The continuous use of polymer materials has exacerbated waste and environmental challenges, spurring a growing interest in eco-friendly polymers, especially biodegradable polymers. These polymers are gaining attention for their potential as antimicrobial agents, particularly in fields like food packaging a need further underscored by the recent COVID-19 pandemic. This study focuses on the development of an antibacterial polymer by combining poly-butylene adipate terephthalate (PBAT) with zinc pyrithione (ZnPt). The antibacterial properties were assessed through turbidity analysis, the shaking flask method, and the film adhesion method. The antibacterial activities of the composites with varying ZnPt% (w/w) contents (0, 0.1, 0.3, and 0.5) were evaluated against Escherichia coli (E. coli) and Staphylococcus aureus (S. aureus). Results revealed that even at a low concentration of 0.1% (w/w), the composites demonstrated significant antibacterial activity against both Gram-positive bacteria (S. aureus) and Gram-negative bacteria (E. coli). Composites with ZnPt concentrations of 0.3% (w/w) or higher achieved over 99.999% antibacterial efficacy. Field emission scanning electron microscopy (FE-SEM) analysis of the fracture surfaces of the composites confirmed the uniform distribution of ZnPt particles, ranging from 1-4 ㎛. Further FE-SEM analysis of bacterial suspensions exposed to the composite surfaces showed clear evidence of cell wall destruction in both E. coli and S. aureus. As an antimicrobial biodegradable polymer, PBAT-ZnPt composites show great promise for applications in various sectors, including food packaging.

• Proceedings of the Korea Association of Crystal Growth Conference
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• 1997.10a
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• pp.99-103
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• 1997

활성탄의 특성을 이용하여 상업적으로 문제시되고 있는 수질 및 공기 정화용 항균성 Ag-활성탄을 제조하여 흡착특성, 표면구조 및 박테리아 저항성에 대하여 조사하였다. 높은 비표면적을 가진 활성탄에 대하여 AgNO$_3$를 사용하여 Ag-활성탄을 제조하였다. 0.1에서 1.0까지의 AgNO$_3$ 몰농도에 침적된 Ag-활성탄의 비표면적 값은 874-1475 $m^2$/g의 범위에 분포하고 있었으며, AgNO$_3$몰농도가 증가함에 따라 비표면적이 작아지는 경향을 나타내어 흡착된 Ag가 원료 활성탄의 표면구조에 영향을 주었다. Ag는 활성탄 표면의 기공 주위에 고르게 분포되었으며 활성탄의 표면에 물리적 흡착에 의해 존재하는 것으로 나타났다. 항균실험을 위하여 박테리아로서 대장균의 일종인 Escherichia coli를 사용하였으며, 흡착된 Ag의 양이 증가됨에 따라 활성의 범위가 증가되었고, Ag가 흡착되지 않은 활성탄의 경우에 있어서는 활성을 전혀 나타내지 않았다.

• Proceedings of the KAIS Fall Conference
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• 2001.05a
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• pp.37-39
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• 2001

구리 이온을 함유한 XCuOㆍ(50-X) Na₂Oㆍ50P₂O/sub 5/ 조성을 승온속도 10K/min로 900℃까지 숭온한 후 1시간동안 유지하여 용융하였으며, 연속적으로 가열되어진 흑연판에 부어 급냉함으로써 모유리를 제조하였다. 제조된 유리를 분말상의 시편으로 만들어 상온에서 증류수로 3일동안 용해시켰다. 각각의 조성에 따른 살균효과를 측정하였으며, 제조된 시편을 소지토에 첨가하여 그 항균특성을 관찰하였다. 항균특성 평가를 위하여 사용된 균주는 Staphylococcus aureus 와 Salmonella typhi가 사용되었으며, Staphylococcus aureus 균주에 대해서는 모든 시편 담지후 3시간 이후에, Salmonella typhi에 대해서는 6시간 이후에 모든 균이 소멸되는 것으로 관찰되었다.

• Microbiology and Biotechnology Letters
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• v.26 no.5
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• pp.373-378
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• 1998

This study was performed to investigate antimicrobial effects and their actions of the ethanol extracts of 12 spices against Lactobacillus plantarum and Leuconostoc mesenteroides, which are related to Kimchi fermentation. The cardamon, thyme, and cumin ethanol extracts among 12 spices showed antimicrobial activities against Lac. plantarum, not against Leu. mesenteroides through paper disc method. The growth of Lac. plantarum was inhibited in MRS broth containing each extracts of cardamon (>4%), thyme (>4%) and cumin (>1%). SDS-PAGE and transmission electron micrographs showed that the cell walls and membranes were disrupted and the cytoplasmic components were leaked in strains treated with ethanol extracts.

• Journal of Korean Ophthalmic Optics Society
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• v.10 no.4
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• pp.261-266
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• 2005

Quaternary ammonium compounds(QACs) are some of the most commonly used antimicrobials. Antimicrobial polymers have been used as biomedical devices. In this Study, We synthesized new methacrylate monomers including quaternary ammonium moieties for preparation of contact lens, And also, we tested for antimicrobial activities against S. aureus, E. coli and P. aeruginosa. They showed much higher antimicrobial activities against all than the polymers of no including quaternary ammonium.

• Proceedings of the Korean Vacuum Society Conference
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• 2016.02a
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• pp.319.2-319.2
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• 2016

ZrN 코팅은 TiN과 특성이 유사한 옅은 금색의 질화 박막이나 내식성이 우수하여 의료용, 자동차 부품, 항공부품, 장식용으로 적용되고 있다. 스테인레스계 의료용 기구나 생활기구의 항균성 기능이 부여된 표면처리는 아직 널리 보급되고 있지 않아 장식성과 내부식성이 뛰어난ZrN 박막에 구리를 함유시킴으로서 항균성의 변화를 관찰하고자 하였다. 진공 아크 이온플레이팅 방법으로서 ZrN을 성장시키면서 스퍼터링법에 의하여 Cu를 함유시키는 방법으로 실험을 실시하였다. 기재는 SUS 304를 사용하였고, 공정온도 $400^{\circ}C$에서 질소분압 1-5 Pa, 아크전류 90 A의 조건에서 Cu 스퍼터링 타겟의 전류를 1-7A 까지 변화하여 구리함유 ZrN 박막을 합성하였다. 구리 함류량에 따른 XRD를 통한 결정구조분석과 SEM/EDX를 통한 성분변화분석을 실시하였다. 구리함유 박막에 대해서 시간에 따른 대장균을 성장을 분석하여 기재인 SUS304, ZrN, 구리 함유 ZrN에 대한 대장균 항균성을 조사하였다.

• Proceedings of the Korea Concrete Institute Conference
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• 2008.11a
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• pp.907-910
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• 2008

Recently sewage facilities mainly consisted of concrete structures are being deteriorated seriously by biochemical reaction originated from sulfur-oxidizing bacteria. To prevent biochemical corrosion of the sewage concrete, antibiotics which inhibit growth of sulfur-oxidizing bacteria have to be developed and applied necessarily. In this study, we are going to introduce technology which biochemical corrosion of sewage facilities concrete could be controlled effectively by antibiosis of antimicrobial concrete