• Journal of the Korea Concrete Institute
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• v.19 no.3
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• pp.313-322
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• 2007

The PCS system, which consists of precast concrete column and steel beam, is a kind of composite structural systems. In this paper, experimental study has been conducted to analyze seismic performance of bolted beam-to-column connections for the PCS system. Based on experimental results from the seismic testing of eight interior PCS specimens, it shows that behavior of PCS system is satisfactory to seismic performance criteria of ACI such as strength deterioration, stiffness degradation and energy dissipation capacity except initial stiffness. All of the specimens maintain their strength at large levels of story drift without significant loss of stiffness and show high ductility level for inelastic behavior. The energy dissipation capacity is two times greater than requirement of ACI criterion. But the initial stiffness of all specimens does not satisfy ACI criterion, and this phenomenon is similar to the other composite structural systems such as RCS, CFT system.

• Journal of Plant Biology
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• v.36 no.4
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• pp.415-423
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• 1993

대두의 uricase II cDNA를 탐침으로 plaque 혼성화 방법에 의해 해녀콩의 뿌리를 cDNA library로부터의 두 개의 phage 클론(λCINUO-01, λCINUO-02)을 선별하였다. 두 phage 클론은 약 1.6 kb와 1.0 kb의 insert를 갖고 있었으며 이들의 염기서열을 결정하기 위하여 pUC19과 pBSKS vector에 subcloing(pcCLNUO-01, pcCLNUO-02)하였다. Sanger법에 의해 염기서열을 결정한 결과, 두 클론은 각각 1,611 bp와 1,024 bp로 이루어져 있었으며 pcCINUO-01은 308개의 아미노산, pcCINUO-02는 301개의 아미노산을 암호화하는 open reading frame(ORF)을 갖고 있었다. 두 클론의 ORF의 염기서열은 대두의 uricase II와 각각 88.9%, 89.3%의 상동성을 보여주었으며, 아미노산 서열은 84.1%, 85.4%의 상동성을 보여주었다. pcCINUO-01의 경우, 종결코돈으로부터 313 NT 하류쪽에 진핵생물의 poly(A) 첨가신호인 AATAAA 서열이 존재하였으며 이로부터 21 NT 하류쪽에 17 잔기의 poly(A)가 존재하였다. 두 클론의 염기서열에서 추정된 아미노산 서열의 카르복시 말단에는 세포질에서 합성된 몇몇 단백질들이 peroxisome으로 수송되는데 필요한 신호서열인 Ser-Lys-Leu-COOH 서열이 존재하고 있었다. 두 클론의 염기서열을 토대로 아미노산 조성을 살펴본 결과, 염기성 아미노산(Arg, His, Lys)과 산성 아미노산(Asp, Glu)이 각각 46 대 35, 47 대 35의 비를 보여주었는데 이는 uricase II 단백질의 염기성 성질을 보여주는 결과로 추정된다. Northern 혼성화 결과 해녀콩에서 uricase II는 뿌리혹에서만 특이적으로 발현됨을 알 수 있었고 게놈 혼성화 반응 결과는 uricase II 유전자가 해녀콩 게놈상에 유전자 가족으로는 존재할 수 있음을 보여주었다.

• KIEAE Journal
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• v.7 no.6
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• pp.113-118
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• 2007

The composite beam adopted in the study was designed to reduce the floor height as well as to embed the top flange of steel frame into the slab that will enable to avoid applying the fire-resistant coating and to unify the joint method with a steel frame-type. As the steel frame and bottom concrete of the beam is pre-fabricated at the factory it could reduce the overall schedule at the jobsite. Applying such composite beam system to the work is expected to provide the efficient and enhanced performance, given the current tendency of the building construction that tends to be getting higher, larger and dense. The study focused on combining the composite beam with various column systems in a bid to propose the details thereof. A desirable composite girder can be adopted depending on site conditions through the evaluation of various beam and jointing approaches. Among the column systems applied to the study are steel column, SRC column, RC-PC column and RC column. The ways of combining with the columns addressed in the study were categorized into the rigid joint, pin joint, steel frame joint and bracket type joint. Besides, the instruction for site fabrication of beam-column was added in an effort to help set up the site fabrication procedures.

• Proceedings of the Korean Society of Broadcast Engineers Conference
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• 2001.11b
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• pp.205-208
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• 2001

최근 스포츠 중계를 중심으로 가상광고가 큰 주목을 받고 있다. 가상광고는 현실에 존재하지 않는 광고를 실영상 내에 삽입하여 마치 실제로 존재하는 물체처럼 보여주는 기술이다. 본 논문에서는 한 대의 카메라로 촬영한 영상으로부터 실시간으로 특징점 추출 및 추적을 행하여, 카메라 Projection 행렬을 복원하고, 이를 이용하여 범용적인 크로마키(chroma-key) 기법으로 영상합성 함으로써 센서와 같은 부가장비가 필요없는 영상기반의 가상광고 시스템을 구현하였다. 일반 PC상에서 알고리즘의 유효성과 실시간 동작성을 확인하였다.

• Restorative Dentistry and Endodontics
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• v.34 no.5
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• pp.430-441
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• 2009

The purpose of this study was to characterize functional distinction between human dental pulp cells(PC) and periodontal ligament cells(PDLC) using cDNA micro array assay and to confirm the results of the microarray assay using RT-PCR. 3 genes out of 51 genes which were found to be more expressed(>2 fold) in PC were selected, and 3 genes out of 19 genes which were found to be more expressed(>2 fold) in PDLC were selected for RT-PCR as well. According to this study, the results were as follows: 1. From the micro array assay, 51 genes were more expressed (2 fold) from PC than PDLC. 2. RT-PCR confirmed that ITGA4 and TGF ${\beta}2$ were more expressed in PC than in PDLC 3. From the micro array assay, 19 genes were more expressed (2 fold) from PDLC than PC. 4. RT-PCR confirmed that LUM, WISP1. and MMP1 were more expressed in PDLC than in PC. From the present study, different expression of the genes between the PC and PDLC were characterized to show the genes which play an important role in dentinogenesis were more expressed from PC than PDLC, while the genes which were related with collagen synthesis were more expressed from PDLC than PC.

• Journal of the Korea Concrete Institute
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• v.17 no.3 s.87
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• pp.445-454
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• 2005

Cracks due to low tensile strength in prestressed concrete (PC) beams may decrease rigidity and structural performance, resulting in excessive deflection. In an effort to solve this problem, in this research, prestressed dual concrete (PDC) has been proposed, consisting of normal strength concrete in compression zone, and high performance steel fiber reinforced concrete(HPSFRC) with a partial depth in tensile zone. Three PDC beams with different depths of HPSFRC and two PC beams were cast for experiments. Analytical models at each stage, i.e., precracking, postcracking, and ultimate, were proposed for analysis of flexural behavior in PDC beams. The experimental results agree well to the analytical ones. Crack formation and its propagation are controlled by the HPSFRC in PDC beams. The initial cracking and service limit loads are increased along with the load carrying capacity and flexural stiffness.

• Journal of Animal Science and Technology
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• v.48 no.5
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• pp.719-726
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• 2006

Bovine whey protein are rich in cysteine, which is the rate limiting amino acid for synthesis of antioxidant glutathione(GSH). Some strains of Lactobacillus caseihas been reported to contain high level of GSH in cell extracts. The objective ofthis study was to determine whether enzymatically hydrolyzed whey protein isolate(WPI) and cell extract of Lb. casei HY2782 could increase intracellular GSH concentrations and protect against oxidant induced cell death in human prostate epithelial cell line (designated as RWPE1, and PC3MMM2 cells). Treatment of RWPE1 cellsandPC3MMM2 cells with hydrolyzed WPI (500g/ml) significantly increased GSH by28.2% and38.4% respectively. Compared with control cells receiving no hydrolyzed WPI(P<0.05). hydrolyzed WPI and Lb casei HY2782 cell extracts significantly protected RWPE1 and PC3MMM2 cellsfrom oxidant induced cell death compared with controls receiving no WPI. DNA damage associated with oxidant treatment was demonstrated by single cell gel (SCG) electrophoresis.

• Journal of the Korean Chemical Society
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• v.34 no.3
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• pp.280-287
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• 1990

The electrochemical behavir of trivalent lanthanides [Sm(Ⅲ), Tb(Ⅲ), and Yb(Ⅲ)) complexes were investigated by the use of direct current, differential pulse polarography and cyclic voltammetry in aprotic media. These reduction peak were irreversible one-electron processes at $E_{pc}$ = -0.16 V, -0.35 V, -0.14 V, and -0.03 V of trivalent lanthanide complexes vs. Ag/AgCl electrode and the behavior of heavier lanthanides were decreased Sm > Tb > Ho > Yb order of the stability constant with decreasing atomic number.

• Journal of Korean Society of Steel Construction
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• v.26 no.1
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• pp.55-68
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• 2014

In this study, the beam-to column connections of concrete-encased-and-filled steel tube columns were tested under cyclic loading. Two specimens using steel beams and two specimens using precast concrete beams were tested. The dimension of the column cross section was $670mm{\pm}670mm$. The beam depths were 488mm and 588mm for the steel beams and 700mm for the precast concrete beams. The longitudinal bar ratios of the precast concrete beams were 1.1% and 1.5%. For the connections to the steel beams, continuity plates were used in the tube columns. For the connections to the PC beams, couplers were used for beam re-bar connections. The test results showed that except for a specimen, deformation capacities of the specimens were greater than 4% rotation angle, which is the requirement for the Special Moment Frame. Particularly, specimens using precast concrete beam showed excellent performances in the strength, deformation, and energy dissipation.

• Journal of the Korea institute for structural maintenance and inspection
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• v.23 no.1
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• pp.36-44
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• 2019

The purpose of this study is to evaluate the structural performance of press-formed type asymmetric column to beam connections of steel-PC composite module frames. Most of the column sections of the joints making up the modular frame use a closed square steel section. The column-beam connection using the closed column section has difficulty in reducing the workability and securing the fire resistance. In order to overcome this disadvantage, concrete is filled in the asymmetrical open type cross section of the steel plate by press forming. A total of four specimens were fabricated to investigate the structural performance of press formed type asymmetric column to beam connections. The experimental results show that the structural performance and behavior of the asymmetric columns are different depending on whether the asymmetric column cross section is composited or the column width thickness ratio. The structural performance of the press formed type asymmetric column to beam connection was evaluated by comparing the experimental results with the theoretical formulas.