• Herbal Formula Science
• /
• v.29 no.1
• /
• pp.45-55
• /
• 2021

Objectives : This study was conducted to evaluate the anti-atrophic effect of polycan in dexamethasone-induced skeletal muscle atrophy in vitro model. Methods : C2C12 myoblast were differentiated into myotube by 2% horese serum medium for 6 days, and then treated polycan extract at different concentrations for 24h. The effect of dexamethasone on the induction of muscle atrophy and expression of atrophy-related genes in differentiated C2C12 myotubes using a GSH, ROS, real-time PCR, western blots analysis. Results : The results showed that Treatment with polycan (100 and 200 ㎍/㎖) noncytotoxic levels on both myoblast and myotube. Polycan decreased the ROS level overproduced with dexamethasone and improved the depletion of GSH level. Dexamethasone showed a decrease in myotube diameter, which was associated with up-regulation muscle-specific ubiquitin ligases markers, such as atrogin-1, FoxO3, myostatin and muscle RING finger-1 (MuRF1), and down-regulation of myogenin, MEF2, Myogenic regulatory factor 5, 6 and MyoD. The results showed that polycan treatment significantly dose-dependently inhibited it. Furthermore, decreased expressions of PI3K/Akt signal pathway by dexamethasone were reversed by treatment with polycan. Conclusions : Thus, polycan suppresses dexamethasone induced muscle atrophy in C2C12 myotube in vitro model through activation of PI3K/Akt pathway and protective effect of improve skeletal muscle function.

• Journal of Physiology & Pathology in Korean Medicine
• /
• v.35 no.1
• /
• pp.22-27
• /
• 2021

This study conducted a questionnaire for students of Pusan National University Graduate School of Korean Medicine who practiced using the Oriental Medicine Diagnosis System (ODS). From the questionnaire, this study investigated current state of application and perception of AI in Korean Medicine and explored the direction of ODS improvement and utilization. The survey questions consisted of six questions examining the satisfaction of the diagnostic expert system, five questions evaluating the availability of the diagnostic expert system, and six questions to predict the impact of AI on the Korean medicine community. The survey analysis showed high satisfaction with practice using ODS. On the other hand, the possibility of using ODS, especially in clinical use, was evaluated as relatively low compared to the satisfaction of the practice. Therefore, the overall impact of AI on the Korean medical community is not expected to be large. Although there are difficulties in standardization of clinical data due to the academic characteristics of Korean medicine, it is necessary to continue attempts to apply AI. By actively introducing educational tools using the latest AI techniques to the diagnosis experience and doctor-patient role in a practice, students will be able to increase their satisfaction with their practice and respond appropriately to the state-of-the-art medical environment.

• The Korea Journal of Herbology
• /
• v.30 no.2
• /
• pp.25-30
• /
• 2015

Objectives : Laver (Porphyra tenera), a red algae species, is one of the most widely consumed edible seaweed in Korea. Laver contains various substances such as essential amino acid, fiber, minerals and polyphenols that benefit human health. In the present study, we prepared ethanol extracts from commercially processed product of Porphyra tenera, and evaluated the growth inhibitory effect against human oral squamous carcinoma YD-10B cells. Methods : Cell viability was measured by MTT assay. Apoptosis was confirmed by TUNEL assay and flow cytometry with the green fluorescent dye FITC annexin V entering apoptotic cells and the red fluorescent dye PI not entering. The expression of the relevant proteins was detected using Western blot. Results : Ethanol extracts of Porphyra tenera (PTE, $50-200{\mu}g/m{\ell}$) caused a significant decrease of cell viability in a dose dependant manner. The cell death occurred as a result of apoptotic process as determined by TUNEL assay and flow cytometric analysis. In line with this observation, decrease in procaspase proteins and increase in cytosolic cytochrome c were observed in cells treated with PTE. In addition, exposure to PTE decreased the expression levels of Bcl-2, and induced PARP cleavage and AIF translocation from mitochondria to nucleus. Conclusions : In conclusion, PTE exerts anti-cancer effects by inducing apoptosis via caspase activation and AIF nuclear translocation in YD-10B cells. These results provide evidence for the possible therapeutic effect of Porphyra tenera in oral cancer cells.

• The Science & Technology
• /
• v.27 no.1 s.296
• /
• pp.39-43
• /
• 1994

한국과학기술단체총연합회는 북한의 과학기술에 대한 실상을 올바로 이해하고 남북간의 협력방안을 모색하기 위한 「북한의 과학기술과 남북교류정책」에 관한 워크숍을 12월8일 타워호텔에서 개최했다. 이날 워크숍에서는 과총이 그동안 북한과학기술의 실상에 관한 연구와 남북한 비교조사연구를 각 분야별로 전문가들에게 의뢰하고 그 연구결과를 순차적으로 발표키로 함에 따라 올해에는 9개분야의 연구결과가 발표됐다. 그중 ▲과학기술정책분야를 비롯 ▲천문학 ▲기상 및 대기환경 ▲원자력 ▲전자공학 및 정보공학 ▲한의학등 6개분야의 연구결과를 요약, 소개한다.

• Journal of the KSME
• /
• v.26 no.1
• /
• pp.3-3
• /
• 1986

과학기술은 체계화된 공학을 가지고 그 전공방향으로하여 공업전문대학, 4년제공과대학 및 대 학원에서 이수, 연구토록 제도적으로 시행되고 있는 것이 실태임은 주지의 사실이며, 현금의 이 제도에서 공업전문대학 출신의 취업문제가 널리 거론되고 있는 마당에 과학기술교육 즉 공학교 육을 주 목표로하는 4년제대학에서의 교과내용을 축소한 듯한(반드시 모든 전문대학에서 그렇치 않으나) 교과종류 및 내용에서 탈피하여, 예를 들면 기계기술교육에서는 전문분야를 세분화하여 열기관전공, 공작기계전공, 유체기계전공 등으로 초년도부터 직시 원리, 기법을 교육하면서 최 소한의 필요불가결한 과학(수학. 물리학등)을 병행교육하므로써 단시일내에 기법을 익히고 또한 과학과 기술이 혼연한 일체라는 기본개념도 터득할 수 있으면서 산업전선의 중간기술지도자를 양성함에도 효율적이라 생각한다.

• Journal of Korean Medical Ki-Gong Academy
• /
• v.4 no.1
• /
• pp.98-118
• /
• 2000

• Journal of the Korean Applied Science and Technology
• /
• v.40 no.6
• /
• pp.1547-1556
• /
• 2023

This study was conducted to check the anti-bacterial ability against Malassezia furfur by mixing Szygium aromaticum and Coptis japonica extracts and to evaluate the antioxidant and anti-inflammatory ability by creating an optimal mixture. Szygium aromaticum and Coptis japonica were extracted with 70% ethanol, 100% methanol, and water to evaluate the antibacterial ability, and it was confirmed that 100% methanol extract of Szygium aromaticum and Coptis japonica water extract had the highest anti-bacterial ability. In addition, when the two extracts were mixed and the anti-bacterial ability was evaluated by ratio, the ratio of 9:1 showed the best activity, and it was confirmed that the antioxidant activity of the mixture was excellent. In Raw 264.7 cells, LPS was used to induce inflammatory responses and confirmed anti-inflammatory activity at 1, 10, 50, and 100 ㎍/mL that did not affect survival, and it was confirmed that NO-production inhibition and IL-6 expression inhibition and COX2 and iNOS protein expression inhibition activity were excellent at 10, 50, and 100 ㎍/mL. Through this study, it is thought that the mixture of Szygium aromaticum100% methanol extract and Coptis japonica water extract can be used as a natural ingredient in functional cosmetics because of its excellent antibacterial and anti-inflammatory effects.

• Proceedings of the Korea Information Processing Society Conference
• /
• 2007.05a
• /
• pp.369-372
• /
• 2007

한의학적 진단 이론은 우수성을 보유하고 있음에도 불구하고 계량화, 정량화되어 시각적으로 표현해 주는 결과를 보이지 못해 서양의학에 비해 뒤떨어져 있는 실정이다. 따라서 본 논문에서는 한의학의 망진을 의료 시스템화 하기 위한 한 단계로서 찰색의 기본이 되는 피부색의 분석을 행하였다. 이를 위해 각 개인의 피부색 차이를 수렴하고 이에 대한 분석을 시행함으로 피부색에 대한 공학적 분석을 시도하였다. 본 논문에서는 객관적으로 피부가 검은 사람과 흰 사람을 비교, 분석하였으며 또한 일반인들을 대상으로 피부색 분석을 실행하여 피부색에 대한 개념을 정립하고자 한다. 마지막으로 이에 대한 평균값을 구하며 망진 시스템의 주된 대상이 되는 황인종의 피부색에 대한 연구를 행하고자 한다.

• Herbal Formula Science
• /
• v.27 no.1
• /
• pp.53-63
• /
• 2019

Objectives : In this study, we investigated the combination effects of Cinnamomi cortex Ethanol Extract (CcEE) and hyperthermia in the human AGS gastric cancer cell line. Methods : AGS cells were treated with the indicated concentrations of CcEE (0, 50 or $60{\mu}g/mL$) for 1h prior to hyperthermia. And then incubated for a further 30 min at the indicated temperatures (37, 42 or $43^{\circ}C$) in a humidified incubator containing 5% $CO_2$ or a thermostatically controlled water bath for hyperthermia. The cell viability was measured by MTT assay, Morphology assay and Trypan blue assay. To investigate the possible molecular signaling pathways, the activation of mitogen-activated protein kinase (MAPK) proteins (ERK, p38 and JNK) and expression of various anti-apoptotic proteins such as Caspase-3, Caspase-9, p53, Cyclin D1 and MMP-2 were assessed by Western blot analysis. In addition, Annexin V and 7-amino-actinomycin D (7-AAD) staining was performed to examine the apoptotic mechanism. Results : Combination of CcEE with hyperthermia effectively suppressed the cell viability and changed cellmorphology compared with CcEE or hyperthermia treatment alone. Combined treatment also abated the expression of Caspase-3, Caspase-9, Cyclin D1 and MMP-2. Whereas, the expression level of p53 was up-regulated by co-treatment. Moreover, combination treatment enhanced phosphorylation of ERK, p38 and JNK. In addition, this combination increased anti-cancer effect by inducing cell death through the apoptosis. Conclusions : Taken together, all these findings suggest that the combination treatment with CcEE and hyperthermia may have therapeutic potential as a promising approach to patients with stomach cancer.

• The Korea Journal of Herbology
• /
• v.38 no.2
• /
• pp.27-34
• /
• 2023

Objectives : Currently, the benign prostatic hyperplasia (BPH) is one of the most common urogenital disorder in old men. We were performed to determine the effects of abietic acid (AC), component of pine resin, in benign prostatic hyperplastic Sprague-Dawley rat (SD rat) induced by testosterone injection (IP). Methods : We monitored body weights in SD rat at start and end date of experiment. After end of experiment, the prostate weights were measured in SD rats. Glutamic oxaloacetic transaminase (GOT) and glutamic pyruvic transaminase (GPT) levels was performed in serum. And we determined the 5-alpha reductase Ⅱ activity, testosterone levels, and dihydrotestosterone levels in prostate tissue and serum using ELISA kit. Results : As results, the prostate wights were increased in BPH group compared to normal group and were decreased in fina, AC30, and AC 50 groups, respectively. Serum GOT levels were decreased in AC50 group compared to BPH group. And Serum GPT levels of AC30 and AC50 groups were lower than BPH group. In addition, the 5-alpha reductase Ⅱ activity, testosterone levels, and dihydrotestosterone levels were decreased the fina, AC10, AC30, and AC 50 groups contrast to the BPH group. Furthermore, 5-alpha reductase Ⅱ activity, testosterone levels, and dihydrotestosterone levels were decreased dose dependent in AC groups compared to BPH group. Conclusion : These results suggest that AC could be used as a potential material for the treatment of BPH by decreasing the androgen levels in benign prostatic hyperplasia model rats.