Journal of the Korean Applied Science and Technology (한국응용과학기술학회지)

The Korean Society of Applied Science and Technology (한국응용과학기술학회)
권호 표지
DOI QR코드

DOI QR Code

Anti-inflammatory and anti-bacterial effects of a mixture of Syzygium aromaticum and Coptis japonica

정향과 황련 혼합물의 항균 및 항염증 활성 연구

  • Eunhong Lee (Department of Pharmaceutical Engineering, Daegu haany University) ;
  • Eun-mi Jung (Department of Pharmaceutical Engineering, Daegu haany University) ;
  • Hyun-Ji Kwon (Advanced Translational Medicine, Konkuk University) ;
  • Jihye Lee (Daegu Haany University Industry Academic Cooperation Foundation) ;
  • BongHyun Woo (BCEL Corp) ;
  • Sungmin Park (AllpassBio) ;
  • Jinhan Park (Department of K-Beauty Business, College of Cosmetics and Pharm, Daegu Haany University) ;
  • Ji Wook Jung (Division of Biotechnology and Convergence, College of Cosmetics and Pharm, Daegu Haany University)
  • 이은홍 (대구한의대학교 일반대학원 제약공학과) ;
  • 정은미 (대구한의대학교 일반대학원 제약공학과) ;
  • 권현지 (건국대학교 일반대학교원 첨단중개의학과) ;
  • 이지혜 (대구한의대학교 산학협력단) ;
  • 우봉현 ((주)비쎌) ;
  • 박성민 (올패스바이오) ;
  • 박진한 (대구한의대학교 화장품제약대학 K-뷰티비즈니스학과) ;
  • 정지욱 (대구한의대학교 화장품제약대학 바이오산업융합학부)
  • Received : 2023.11.23
  • Accepted : 2023.12.29
  • Published : 2023.12.30
Download PDF
⟨ Previous Next ⟩

Abstract

This study was conducted to check the anti-bacterial ability against Malassezia furfur by mixing Szygium aromaticum and Coptis japonica extracts and to evaluate the antioxidant and anti-inflammatory ability by creating an optimal mixture. Szygium aromaticum and Coptis japonica were extracted with 70% ethanol, 100% methanol, and water to evaluate the antibacterial ability, and it was confirmed that 100% methanol extract of Szygium aromaticum and Coptis japonica water extract had the highest anti-bacterial ability. In addition, when the two extracts were mixed and the anti-bacterial ability was evaluated by ratio, the ratio of 9:1 showed the best activity, and it was confirmed that the antioxidant activity of the mixture was excellent. In Raw 264.7 cells, LPS was used to induce inflammatory responses and confirmed anti-inflammatory activity at 1, 10, 50, and 100 ㎍/mL that did not affect survival, and it was confirmed that NO-production inhibition and IL-6 expression inhibition and COX2 and iNOS protein expression inhibition activity were excellent at 10, 50, and 100 ㎍/mL. Through this study, it is thought that the mixture of Szygium aromaticum100% methanol extract and Coptis japonica water extract can be used as a natural ingredient in functional cosmetics because of its excellent antibacterial and anti-inflammatory effects.

본 연구는 정향, 황련 추출물을 혼합하여 Malassezia furfur에 대한 항균 능력을 확인하고 최적의 혼합물을 만들어 항산화 및 항염증 능력을 평가하기 위해 수행되었다. 정향과 황련을 70% 에탄올, 100% 메탄올, 열수로 추출하여 항균 능력을 평가하여 정향은 100% 메탄올 추출물, 황련은 열수 추출물이 가장 항균 능력이 높은 것을 확인하였다. 또한 두 추출물을 혼합하여 비율별로 항균 능력을 평가하였을 때 9:1 비율이 가장 우수한 활성을 보였고 혼합물의 항산화 활성이 뛰어남을 확인하였다. Raw 264.7 세포에서 생존율에 영향을 미치지 않는 1, 10, 50, 100 ㎍/mL에서 LPS를 이용하여 염증 반응을 유도하고 항염증 활성을 확인한 결과 10, 50, 100 ㎍/mL에서 No 생성 저해율과 IL-6 발현 저해율 및 COX2, iNOS 단백질 발현 저해 활성이 뛰어남을 확인하였다. 본 연구를 통해 정향 메탄올 추출물 및 황련 열수 추출물의 혼합물은 항균, 항염증 효능이 뛰어나기에 기능성 화장품의 천연 소재로써 사용이 가능할 것으로 사료된다.

Keywords

Acknowledgement

본 연구는 2022년도 중소벤처기업부의 기술개발사업 지원에 의한 연구입니다. [S3308484] 본 연구는 중소기업기술정보진흥원의 "창업성장기술개발사업(디딤돌, S3308484)"사업의 지원을 받아 수행된 연구결과입니다.

References

  1. Y. M. Yoon, S. H. Bae, S. K. An, Y. B. Choe, K. J. Ahn and I. S. An, "Effects of Ultraviolet Radiation on the Skin and Skin Cell Signaling Pathways", Kor. J. Aesthet. Cosmetol, Vol.11, No.3 pp. 417-426, (2013). 
  2. S. M. Dang and E. S. Lee, "The Effect of a Scalp Type and Awareness on Self-scalp Management Behavior, Kor. J. Aesthet. Cosmetol, Vol.11, No.6 pp. 1147-1153, (2013). 
  3. M. S. Lee, J. S. Joung and D. Y. Park, "The Effects of Arctium lappa L. Root Extracts on the Scalp and Hair", Kor. J. Aesthet. Cosmetol, Vol.13, No.1 pp. 43-48, (2015). 
  4. J. S. Han, "Scalp Demodex alopecia have a decisive effect", Kor. J. Aesthet. Cosmetol, Vol.9, No.1 pp. 1, (2011). 
  5. J. Y. Um, B. M. Jin and Y. K. Cho. "Isolation and Identification of Antifungal Compounds against Malassezia species from Cinnamomoum cassia Blume.", Korean Society of Cosmetics and Cosmetology, Vol.10, No.3 pp. 331-344, (2020). 
  6. S. K. Cho, H. J. Kim, W. Y. Chung and E. J. Hwang. "A Scalp Keratin Detection Method Based on Atrous Convolution and Faster R-CNN for Diagnosis of Seborrheic Scalpitis", KTCP, Vol.27, No.9 pp. 440-445, (2021). 
  7. K. A. Seo, and S. H. Li, "A Study on the Anti-bacterial Effect and Dandruff Scalp Emprovement of Malassezia furfur of Chamaecyparis obtusa", Kor J Aesthet Cosmetol, Vol.13, No.3 pp. 285-293, (2015). 
  8. I. H. Lee, M. J. Kim, J. H. Choi, C. H. Kim, and S. H. Choi, "Antifungal effect of bilobalide and ginkgolide extracted from leaves of Ginkgo biloba against Pityrosporum ovale", KSBB Journal, Vol.25, No.2 pp. 173-178, (2010). 
  9. Y. M. Ha, B. B. Lee, H. J. Bae, K. M. Je, S. R. Kim, J. S. Choi, and I. S. Choi, "Anti-microbial activity of grapefruit seed extract and processed sulfur solution against human skin pathogens.", Journal of Life Science, Vol.19, No.1 pp. 94-100, (2009). 
  10. J. M. Lee, and J. S. Kim, "The Effect of Shampoo and Mist on the Seborrheic Oil Scalp.", Kor J Aesthet Cosmetol, Vol.12, No.4 pp. 526-531, (2014). 
  11. H. Yang, K. H. Oh, and Y. C. Yoo, "Anti-inflammatory effect of hot water extract of aronia fruits in LPS-stimulated RAW 264.7 macrophages.", Journal of the Korean Society of Food Science and Nutrition, Vol.44, No.1 pp. 7-13, (2015). 
  12. H. Jeon, J. Y. Lee, S. H. Ko, Y. J. Lee, S. Y. Lee, H. J. Park, and T Y. Shin. "Anti-inflammatory Effect of MeOH Extract of Cibotium barometz in IFN-γ and LPS-stimulated Mouse Peritoneal Macrophage.", Korean Journal of Pharmacognosy, Vol.41, No.2 pp. 108-114, (2010). 
  13. R. G. Leuschner, and J. Zamparini, "Effects of spices on growth and survival of Escherichia coli 0157 and Salmonella enterica serovar Enteritidis in broth model systems and mayonnaise.", Food Control, Vol.13, No.6-7 pp. 399-404, (2002). 
  14. J. Jang, D. H. Kang, J. Yoon, and H. S. Kim, "Separation and Purification of Antimicrobial Substance from Syzygium aromaticum Merrill et Perry for Treatment of Microbial Vaginosis.", Korean Society for Biotechnology and Bioengineering Journal, Vol.33, No.4 pp. 285-292, (2018). 
  15. H. H. Leem, E. O. Kim, M. J. Seo, and S. W. Choi, "Antioxidant and anti-inflammatory activities of eugenol and its derivatives from clove (Eugenia caryophyllata Thunb.)", Journal of the Korean Society of Food Science and Nutrition, Vol.40, No.10 pp. 1361-1370, (2011). 
  16. N. Kim, and J. Kim, "Preparation of Hydrophobic Antimicrobal Compounds Encapsulated Nanoparticles Using Alkoxysilane-functionalized Amphiphilic Polymer Precursor and Their Antimicrobial Properties.", Journal of Adhesion and Interface, Vol.18, No.1 pp. 13-24, (2017). 
  17. Y. H. Kim, and B. S. Park, "The effect of eugenol on the induction of apoptosis in HSC-2 human oral squamous cell carcinoma.", Journal of Korean Society of Dental Hygiene, Vol.15, No.3 pp. 523-529, (2015). 
  18. S. H. Kim, T. Y. Shin, H. Y. Kim, Y. M. Lee, E. H. Lee, B. K.Shin, and H. M. Kim, "Inhibition of immediate allergic reaction by eugenol." Yakhak Hoeji, Vol.40, No.6 pp. 679-683, (1996). 
  19. H. K. Kim, and S. U. Hong, "The anti-inflammatory effects of Huang-Lyun (Coptidis Rhizoma, CR) on injured tissue after burn elicitation.:, The Journal of Korean Medicine, Vol.32, No.2 pp. 1-13, (2011). 
  20. E. H. Kim, Y. A. Jang, S. B. Kim, H. H. Kim, and J. T. Lee, "Antimicrobial, antifungal effect and safety verification using BCOP assay of extracts from Coptis japonica.", Journal of Applied Biological Chemistry, Vol.61, No.3 pp. 297-304, (2018). 
  21. J. W. Lee, H. S. Han, and Y. J. Lee, "Anti-inflammatory effect of Coptidis Rhizoma extract.", The Korea Journal of Herbology, Vol.29, No.5 pp. 83-90, (2014). 
  22. S. K. Lee, I. Lee, S. H. Shin, E. Y. Kim, and B. C. Shin, "Effects of Coptidis Rhizoma on the Anti-inflammation and Motor Recovery in Photothrombotic Brain Infarction Model in Rats", The Korea Journal of Herbology, Vol.24, No.1 pp. 179-189, (2009). 
  23. M. S. Blois , "Antioxidant determinations by the use of a stable free radical", Nature, Vol.181, pp. 1199-1200, (1958). 
  24. R. Re, N. Pellegrini, A. Proteggente, A. Pannala, M. Yang, C. Rice-Evans, "Antioxidant activity applying an improved ABTS radical cation decolorization assay", Free Radical Biology Medicine, Vol.26, No.9-10 pp. 1231-1237, (1999). 
  25. Folin, O. and Denis, W. "On phosphotungstic-phosphomolybdic compounds as color reagents.", Journal of biological chemistry, Vol.12, No.2 pp. 239-243, (1912). 
  26. M. Oyaizu, "Studies on products of browning reaction antioxidative activities of products of browning reaction prepared from glucosamine", The Japanese journal of nutrition and dietetics, Vol.44, No.6 pp. 307-315, (1986). 
  27. O. H. Lec, S. H. Jung, and J. Y. Son, "Antimicrobial activity of clove extract by extraction solvents.", J. Korean Soc Food Sci Nutr, Vol.33, No.3 pp. 494-499, (2004). 
  28. J. H. Kim, S. J. Kwack, Y. K. Cho, B. K. Kim, J. G. Kim, E. Lee, and K. J. Kim, "The effect of Coptidis rhizoma, Chinese galls and phytoncide in Malassezia furfur.", Journal of Life Science, Vol.25, No.7 pp. 748-756, (2015). 
  29. E. J. Kim, J. Y. Choi, M. R. Yu, M. Y. Kim, S. H. Lee, and B. H. Lee, "Total polyphenols, total flavonoid contents, and antioxidant activity of Korean natural and medicinal plants.", Korean Journal of Food Science and Technology, Vol.44, No.3 pp. 337-342, (2012). 
  30. Y. M. Park, S. J. Kim, K. H. Jo, E. J. Yang, and S. T. Jung, "Anticariogenic and antioxidant activities from medicinal herbs.", Journal of the Korean Society of Food Science and Nutrition, Vol.35, No.3 pp. 284-293, (2006). 
  31. S. H. Shin, and S. M. Kan, "The antioxidation effect of brown algae extract.", Journal of the Korean Society of Cosmetology, Vol.27, No.4 pp. 851-858, (2021). 
  32. N. Y. Park, D. G. Han, GE-GE, M. J. Bae, H. J. Kim, S. G. Kim, E. Y. Choi, and B. J. An, "Verification of antioxidant activity from velvet bean (Mucuna pruriens) extract.", J. Soc. Cosmet. Sci. Korea, Vol.49, No.1 pp. 9, (2023) 
  33. M. Y. Lee, M. S. Yoo, Y. J. Whang, Y. J. Jin, M. H. Hong, and Y. H. Pyo, "Vitamin C, total polyphenol, flavonoid contents and antioxidant capacity of several fruit peels.", Korean Journal of Food Science and Technology, Vol.44, No.5 pp. 540-544, (2012). 
  34. Y. H. Kim, Y. J. Lee, S. O. Park, S. J. Lee, and O. H. Lee, "Antioxidant compounds and antioxidant activities of fermented black rice and its fractions.", Korean Journal of Food Science and Technology, Vol.45, No.2 pp. 262-266, (2013). 
  35. H. S. Song, Y. H. Park, S. K. Kim, W. K. Moon, D. W. Kim, and K. Y. Moon, "Downregulatory effect of extracts from mistletoe (Viscum album) and pueraria root (Pueraria radix) on cellular NF-κB activation and heir antioxidant activity.", Journal of the Korean Society of Food Science and Nutrition, Vol.33, No.10 pp. 1594-1600, (2004). 
  36. J. H. Hong, E. S. Jang, M. C. Gil, G. W. Lee, and Y. H. Cho, "Anti-inflammatory Effects of Rumohra adiantiformis Extracts Fermented with Bovista plumbea Mycelium in LPS-stimulated RAW 264.7 Cells.", Journal of Life Science, Vol.33, No.6 pp. 471-480, (2023). 
  37. Y. J. Choi, M. H. Park, Y. S. Kim, and K. I. Jung, "Antioxidant and anti-inflammatory effects of Abies holophylla leaf extract in LPS-induced RAW 264.7 cells.", J Korean Soc Food Sci Nutr, Vol.9, No.6 pp. 569-577, (2020). 
  38. B. K. Kang, K. B. W. R. Kim, M. J. Kim, S. W. Bark, W. M. Pak, B. R. Kim, and D. H. Ahn, "Anti-inflammatory activity of an ethanol extract of Laminaria japonica root on lipopolysaccharide-induced inflammatory responses in RAW 264.7 cells.", Korean Journal of Food Science and Technology, Vol.46, No.6 pp. 729-733, (2014). 
  39. Y. J. Surh, K. S. Chun, H. H. Cha, S. S. Han, Y. S. Keum, K. K. Park, and S. S. Lee, "Molecular mechanisms underlying chemopreventive activities of anti-inflammatory phytochemicals: down-regulation of COX-2 and iNOS through suppression of NF-κB activation.", Mutation Research/Fundamental and Molecular Mechanisms of Mutagenesis, Vol.480, pp. 243-268, (2001). 
  40. C. S. Kwak, and H. I. Choi, "In vitro antioxidant and anti-Inflammatory activities of ethanol extract and sequential fractions of flowers of Prunus persica in LPS-stimulated RAW 264.7 macrophages.", Journal of the Korean Society of Food Science and Nutrition, Vol.44, No.10 pp. 1439-1449, (2015).