• 동의생리병리학회지
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• 제35권6호
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• pp.211-218
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• 2021

In order to increase the clinical value of an identification of patterns according to the eight principles (IPEP) in Korean medicine practice, The research on the Clinical Practice Guideline (CPG) of IPEP should comprehend the situation of clinical usage of IPEP practiced by Korean medicine doctors at first. Google survey form were emailed to Korean Medicine doctors registered in the Association of Korean Medicine on 04/15/2021 and the survey was closed at 04/22/2021. Data of 505 answered cases were analyzed by Frequency analysis, Chi-Square analysis, correlation analysis for understanding differences by groups. Out of 505 respondents, 57.6% have answered that they are using IPEP. It means that 42.4% of KM doctors don't use in the medical practice reversely in spite of fundamental diagnostic theory. The 64.7% respondents of no using IPEP presented their opinion about the theoretical problem that it is difficult to use because the concept of IPEP is ambiguous. And next, the 52.1% of the respondents expressed that there is no objective tools to measure and record the IPEP evidences in actual implementation. And 49.6% of the respondents also suggested that it is hard to trust and use IPEP similar to the previous comment. Even about 50% of the respondents are carrying out diagnosis and treatment using IPEP, it showed that there were several unsolved problems such as lack of understanding and practical tools or objective indicators for diagnosis of IPEP. Through the above results, the concept, usage, measurement requirements with indices and discriminant logic of IPEP were manifested as the main hopes of attending members of Korean medicine in the survey, so the IPEP CPG should make clear about these difficult but necessary assignment in the near future.

• 대한한의학방제학회지
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• 제31권4호
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• pp.231-243
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• 2023

Objectives : Jasminum officinale L. var. grandiflorum is used as a traditional or folk remedy in China to treat arthritis, hepatitis, duodenitis, conjunctivitis, gastritis, and diarrhea. In this study, we aimed to study the hepatocyte protective activity and molecular mechanism of Jasminum officinale L. var. grandiflorum aqueous extract (JGW) using HepG2 hepatocyte cell lines. Methods : HepG2 cells were pretreated with diverse concentrations of JGW, and then the cells were exposed to tert-butyl hydroperoxide (tBHP) for inducing oxidative stress. Hydrogen peroxide (H₂O₂) production, glutathione (GSH) concentration, mitochondrial membrane potential (MMP) and cell viability were measured to investigate hepato-protective effects of JGW. Phosphorylation of AMP-activated protein kinases (AMPK), acetyl coenzyme A carboxylase (ACC) and effects of compound C on cell viability were examined to observe the role of AMPK on JGW-mediated cytoprotection. Results : Pretreatment with JGW (10-300 ㎍/mL) significantly suppressed cytotoxicity induced by tBHP in a concentration dependent manner and reduced the expression of cleaved PARP and cleaved caspase-3 proteins related to apoptosis in HepG2 cells. In addition, pretreatment with JGW significantly prevented the increase in H₂O₂ production, GSH depletion, and lower MMP induced by tBHP. Treatment with JGW (30 minutes of incubation and concentrations of 100 and 300 ㎍/mL) increased the phosphorylation of AMPK and ACC and treatment with compound C, a chemical inhibitor of AMPK, inhibited the cytoprotective effect of JGW. Conclusions : Our results demonstrated that JGW may protect hepatocytes from oxidative stress via activation of AMPK.

• 한국정보처리학회:학술대회논문집
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• 한국정보처리학회 2009년도 춘계학술발표대회
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• pp.78-81
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• 2009

한의학에서 신장(腎臟)은 단순히 소변을 만들어내는 곳으로 보는 것이 아니라 정력과 생식 활동을 담당하는 곳으로 표현하고 있다. 이러한 신장은 두 개의 강낭콩처럼 생긴 모양을 띄고 있으며 서로 마주 보고 있고 등에 붙어 있어 체내의 조절 기능을 수행하고 있다. 이와 같은 역할을 수행하는 신장에 이상이 발생하면 신부전, 신장염, 신우염 및 부종 등의 신장 질환을 앓게 된다. 따라서 본 논문에서는 신장 기능의 이상 유무를 손쉽게 판단하기 위한 방법으로 음성 분석을 통한 자가 진단법을 제안하고자 한다. 이를 위해 한의학에서 제시하고 있는 신장 질환자에 대한 목소리 패턴을 객관적 지표로 설계하기 위해 정상인과 신장 질환자를 대상으로 음성의 고저(高低)에 해당하는 분석 요소를 적용하여 피실험자 집단간의 비교, 분석을 실험으로 입증하고자 한다.

• 동의생리병리학회지
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• 제35권6호
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• pp.274-279
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• 2021

This study was conducted to find objective diagnostic indicators for the Eight Principle Pattern Identification using a pulse wave analyzer. Typology Complexion Pulse and Symptom data from 500 women over the age of 18 were used. Five experts made a diagnosis of Eight Principle Pattern Identification and divided the subjects into 'Yin-Yang', 'Exterior-Interior', 'Cold-Heat', 'Deficiency-Excess' groups. Their pulse waves were measured in the left and right radial arteries, and it was investigated whether there was a significant difference between groups in the pulse wave parameter values. 'Yin' group showed a significantly lower value in the left radial artery for Ener, Emin, EIX, T4T, T4TT parameters and in the Right for T2, T2T, T5. The Vmag, As and Ad parameters were significantly different between the 'Exterior' and 'Interior' groups. 'Heat' group showed a significantly higher value in the right radial artery for RAI/t parameter. 'Deficiency' group showed a significantly higher value in the right radial artery for W, Angl parameters. Through this study, significant pulse wave parameters were found, and they can be used as objective diagnostic indicators for Eight Principle Pattern Identification.

• 한방안이비인후피부과학회지
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• 제34권4호
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• pp.1-11
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• 2021

Objectives : The aim of this study was to investigated the anti-aging and anti-wrinkles effects of the pinus koreaiensis seed(PKS) oil. Methods : The anti-oxidant effect was performed by beta-carotene bleaching assay and the intracellular proteome was analyzed expression of each 15 proteins by 2-D electrophoresis. And fatty acid was analysed by gas chromatography. Anti-wrinkle effect was analyzing human skin by the PRIMOS system. Results : Fatty acid analysis of PKS oil has shown oleic acid was 49.7% and linoleic acid was 34.1%. And the antioxidant effect was about 125% compared with alpha-tocoperol(0.1%) by beta carotene bleaching assay. In 2D PAGE analysis, fifteen protein changes in five mechanisms which was collagen synthesis pathway, MMPs, ECM-cell interaction, cytokine, antioxidant enzymes were analyzed. In case of anti-wrinkle effect was proved in vivo by analyzing human skin by the PRIMOS system. The analysis results of eye wrinkles for 4 weeks showed an improvement effect of over 6%. Conclusions : In this study, the amount of protein change in the five mechanism through the cell experiment and the skin anti wrinkle efficacy by the human in vivo test were investigated. As a result pinus koreaiensis seed oil by supercritical extraction could be used as a anti-aging and anti-wrinkle substance for the skin.

• 대한한의정보학회지
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• 제13권1호
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• pp.14-25
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• 2007

기공시유구역사전통적문화(氣功是悠久歷史傳統的文化), 해탈생로병사고통적수련법(解脫生老病死苦痛的修練法), 거병건신(祛病健身), 심신단련(心身鍛鍊), 수명장수적양생법(壽命長壽的養生法). "기공"("氣功") 취시행기공부(就是行氣功夫), 이수련수행(以修練修行), 예방정신화육체질병(豫防精神和肉體疾病), 건강신체(健康身體), 연년익수(延年益壽), 기공적수련방법(氣功的修練方法), 유수형태(有數形態), 육체화정신적건강(肉體和精神的健康), 시의료기공(是醫療氣功), 의료기공시검토점(醫療氣功是檢討點), 통과고대의서적정리(通過古代醫書的定理). 의료기공시다개형태변화(醫療氣功是多個形態變化), 불교전파화일기(佛敎傳播和一起), 불가기공취재일개문파불가수련활동중형성(佛家氣功就在一個門派佛家修練活動中形成). 불가기공시(佛家氣功是), 수행자소추구적(修行者所追究的), 유지건강육체적기공법(維持健康肉體的氣功法). 의료기공적모습화구도적모습(醫療氣功的模襲和求道的模襲), 시혼합형태(是混合形態). 현재(現在) 불가기공지원류(佛家氣功之原流) 가이설시소림역근경(可以說是少林易筋經). 저본논고취시이의료기공적관점래접근소림역근경(這本論考就是以醫療氣功的觀點來接近少林易筋經).

• 대한본초학회지
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• 제38권1호
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• pp.21-30
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• 2023

Objective : Gardeniae Fructus (GF) has bitter and cold nature. Thus, it has been traditionally prescribed in processed form roasted with ginger juice for patients with a weak stomach. This study investigated the effects of processed GF in tert-butyl hydroperoxide (tBHP)-treated gastric epithelial cells. Methods : Processed GF was made by applying 40% ginger juice or 10% ethanol for 24 h and then roasting at 150℃ for 5 minutes. Apoptosis was determined by terminal deoxynucleotidyl transferase dUTP nick end labeling (TUNEL) assay. Mitochondrial membrane potential (MMP) was monitored by flow cytometry using the membrane permeable fluorescent dye Rh123. Protein expression was measured by Western blot analysis. Results : Cell viability was reduced by tBHP and restored by ethanol extract of GF (GFE). In the TUNEL assay, it was found that cell death by tBHP was due to apoptosis, and GFE had an anti-apoptotic effect. Processed GF roasted with ginger juice showed the best anti-apoptotic effect. Processed GF also inhibited MMP loss and restored tBHP-induced changes in expression levels of apoptosis-related proteins. Increased ROS production and GSH depletion after tBHP treatment were significantly reduced by processed GF. In addition, tBHP-induced activation of mitogen-activated protein kinase (MAPK) was inhibited by processed GF. Conclusion : These results demonstrate that the processed GF is able to protect gastric epithelial cells from oxidative stress-induced cell death with antiapoptotic and antioxidant activity. In addition, it shows that the processing of GF, which have been traditionally used for gastrointestinal protection, partially have scientific validity.

• 대한의료기공학회지
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• 제9권1호
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• pp.238-274
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• 2006

• 대한의료기공학회지
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• 제9권1호
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• pp.110-157
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• 2006

• 대한의료기공학회지
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• 제4권1호
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• pp.179-200
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• 2000