The Korea Journal of Herbology (대한본초학회지)

The Korea Association of Herbology (대한본초학회)
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Cytoprotective effect of the processed Gardeniae Fructus on oxidative stress-induced gastric epithelial cells

산화스트레스를 유발한 위 상피세포에서 수치 치자의 세포 보호 효과

  • Jong Rok, Lee (Department of Pharmaceutical Engineering, Daegu Haany University) ;
  • Sang Chan, Kim (College of Korean Medicine, Daegu Haany University) ;
  • Sook Jahr, Park (Department of Pharmaceutical Engineering, Daegu Haany University)
  • 이종록 (대구한의대학교 제약공학과) ;
  • 김상찬 (대구한의대학교 한의과대학) ;
  • 박숙자 (대구한의대학교 제약공학과)
  • Received : 2022.12.09
  • Accepted : 2023.01.25
  • Published : 2023.01.30
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Abstract

Objective : Gardeniae Fructus (GF) has bitter and cold nature. Thus, it has been traditionally prescribed in processed form roasted with ginger juice for patients with a weak stomach. This study investigated the effects of processed GF in tert-butyl hydroperoxide (tBHP)-treated gastric epithelial cells. Methods : Processed GF was made by applying 40% ginger juice or 10% ethanol for 24 h and then roasting at 150℃ for 5 minutes. Apoptosis was determined by terminal deoxynucleotidyl transferase dUTP nick end labeling (TUNEL) assay. Mitochondrial membrane potential (MMP) was monitored by flow cytometry using the membrane permeable fluorescent dye Rh123. Protein expression was measured by Western blot analysis. Results : Cell viability was reduced by tBHP and restored by ethanol extract of GF (GFE). In the TUNEL assay, it was found that cell death by tBHP was due to apoptosis, and GFE had an anti-apoptotic effect. Processed GF roasted with ginger juice showed the best anti-apoptotic effect. Processed GF also inhibited MMP loss and restored tBHP-induced changes in expression levels of apoptosis-related proteins. Increased ROS production and GSH depletion after tBHP treatment were significantly reduced by processed GF. In addition, tBHP-induced activation of mitogen-activated protein kinase (MAPK) was inhibited by processed GF. Conclusion : These results demonstrate that the processed GF is able to protect gastric epithelial cells from oxidative stress-induced cell death with antiapoptotic and antioxidant activity. In addition, it shows that the processing of GF, which have been traditionally used for gastrointestinal protection, partially have scientific validity.

Keywords

Acknowledgement

This work was supported by the National Research Foundation of Korea (NRF) grant funded by the Korea government (MOE) (No. 2017R1D1A3B03031863).

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