• Parasites, Hosts and Diseases
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• v.23 no.2
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• pp.214-220
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• 1985

Four cases of human infection by Echinochasmus japonicus (Trematoda; Echinostomatidae) were proven by the recovery of adult worms after a treatment with praziquantel (10mg/kg in single dose) and purgation in February 1984 in Korea. The patients were 3 middle-aged men and 1 elderly woman residing in a riverside village of Kangjin-gun (Tamjin river), Jeonranam-do, an ever-known endemic area of metagonimiasis. The treatment revealed numerous M. yokogawai worms together with 1-43 (total 54) specimens of E. japonicus and/or a few number of other kinds of trematodes or cestodes. The patients had gastrointestinal troubles such as indigestion and abdominal discomfort, probably due to these fluke infections. They have been eating some raw flesh of fresh water fishes such as cyprinoid ones or sweet fish caught from the river. This is the first record of human E. japonicus infection in the literature.

• Parasites, Hosts and Diseases
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• v.36 no.1
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• pp.55-58
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• 1998

A small-scale epidemiological survey was undertaken on the residents along the Mekong River near Pakse, Laos, to know the status of helminthic infections. A total of 137 fecal samples were collected from the staffs of the provincial government, their family, and primary schoolchildren in Pakse City, Champassak Province, and examined by Kato- katz smear technique. The overall helminth positive rate was 75.9%, and the helminths detected were Opisthorchis viuerrini (43.8%), Asccris lumbricoines (26.3%), fichuris trichiurn (19.0%), hookworms (19.0%), Strongyle컸os stercoraLis (2.2%), Taenio sp. (0.7%), and Schistosoma mekongi (1.5%). To obtain the adult worm of the liver fluke, three infected persons were treated with praziquantel and purged with magnesium sulfate. Five, 10, and 395 adult flukes, respectively, were collected from their diarrheic stools, all of which were morphologically identified as 0. viueni,ni. The results represent that the liver fluke and soil-transmitted helminths are highly prevalent, and the life cycle of 5. mekowi is likely to be maintained in this area.

• KOREAN JOURNAL OF CROP SCIENCE
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• v.7 no.1
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• pp.153-160
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• 1969

Experiments on sweet sorghum(sorgo) with different population per unit area were conducted from 1966 to 1968 for the first time in Korea. By increasing plant population stalk weight, refinable sugar and seed weight per plot were increased, but stalk weight per plant, brix percentage, sugar content, stem diameter and 1, 000 seed weight were decreased. Plant height, maturity and lodging were not affected by the treatments. The result obtained has suggested that the effects of plant spacing within row on the characteristics of plant growth and on yields were greater than those of row width. Negative correlations existed between sugar content and sugar yield, and seed weight per plant and seed yield. The optimal plant population in this study ranged from 16, 700 to 22, 200 plants per 10a, row width of 60cm and plant spacing in row of 15 to 20cm resulted in the highest sugar and seed.

• Journal of the Korea Convergence Society
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• v.4 no.2
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• pp.9-13
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• 2013

This study which improve pretreatment method was to increase effective diagnosis of barium enema to remind a more accurate action by training precautions, method of taking medicine, time and taking suitable laxative to patient. First, A total of 504 patients who received barium enema in the E university hospital were evaluated about repretreatment proportion of patients. 176 patients who were changed with precaution were evaluated about repretreatment. Second, Both 130 patients who were not changed with the type and amount of laxative and 137 patients who were changed with it were evaluated. Repretreatment rate was reduced about 10% since changed precautions. Stomachache was reduced about 21% due to chage methode to take the laxative improved. Patients who think cleanliness degree of bowel increased that it is going very well about 11.9% since improvement and decreased that it's not bad about 16.3%. The methods which accurately recognize precautions to patient decrease repretreatment rate, inconvenience and pain of patients due to repretreatment. Expectation mentlity for accurate inspection also had increased in that patients think that cleanliness degree of bowel was increased.

• Parasites, Hosts and Diseases
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• v.23 no.1
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• pp.33-40
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• 1985

Two cases of human Erhinostoma hortense infection and their probable infection source were identified by prasiqantel (Distocide) treatment of the patients and by examining two kinds of fresh water fish which were eaten raw by them. The result of the research can be summarized as follows: 1. The patients, each aged 31 and 30, were residing in the same house in Wonju City, Kangwon Province. The first case was hospitalized due to epidemic hemorrhagic fever (E.H. fever) and the second case was healthy but had slight degree of abdominal pain and diarrhea from time to time. In the stool examination, eggs of 5. hortense ($114.3{\times}71.0{\mu\textrm{m}}$) average from the first case and $119.1{\times}68.3{\mu\textrm{m}}$ average from the second) were found. By administering single dose of praziquantel (10~15mg/kg) and purgation with magnesium salt to them. sin adults of 5. hortense were collected from the diarrheal stools of the second case. 2. By examining 8:k Moroco oxycephalus and 20 Carassius carassius which were captured at the place where the two patients had captured and eaten the fresh water fish, the metacercariae of Echinostoma sp. were found from 3 (3.5%) M. oxycephalus. 3. After the experimental infection of 3 isolated metacercariae to one albino rat three adults of 5. hortense were recovered. By the present study, the two patients revealing the echinostomatid eggs in their stools were proven to be infected with 5. hortense and to be the second and third human cases of this luke infection in Korea. Mcroco oxycephalus harboured the metacercariae of E. hortense and appeared to be a new second intermediate host.

• Parasites, Hosts and Diseases
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• v.22 no.1
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• pp.51-60
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• 1984

Two cases of natural human infection by Heterophyopsis continua (Heterophyidae) were identified by collection of adult worms in 1983 in Korea. And in order to know the source of infection a study on fish intermediate hosts was performed along the southwestern coastal areas. The cases were 21 and 50 year old males residing in southern coastal areas. They had the clinical complaints of non-specific gastrointestinal symptoms such as epigastric pain and one of them also complained the discharge of tapeworm segments. Praziquantel in single dose of 10~15mg/kg was given followed by purgation with magnesium salt and 2 and 46 specimens of H. continua were collected from the diarrheal stools. They said to have eaten raw ilesh of several kinds of brackish water fishes. The results of fish examination for metacercarial infection were as follows: I. Seventeen (663.0%) out of 27 perches (Lateolabrax japonicus) and 10 (29.4%) out of 34 gobies (Acantkogobius flavimanus) harboured the metacercariae but none of 42 mullets (Mugil cepkalus) were found to harbour them. 2. The average metacercarial density in perches was 55.1 and 18.7 per fish respectively. And the majority of the metacercariae were collected from the muscle of body portions in these fishes. 3. After the metaeercariae were experimentally fed to two rats and one young dog. two adult worms were recovered from the dog while none from the rats. These worms were also identified as H. continua. From the results it was concluded that the brackish water flshes which the human cases said to have eaten were the source of infection.

• Parasites, Hosts and Diseases
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• v.35 no.4
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• pp.283-290
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• 1997

A total of 32 human diphyllobothriasis cases have been reported so far in Korea, exclud- ing 21 egg-positive cases from stool examinations. Authors experienced five more human cases of DiphHllobothrium latum infection, especially infected due to eating raw flesh of redlip mullet, Lizc hoematocheiln. Five cases were neighbors residing in the Puchon area, Kyonggi-do, who ate raw mullets (L. hQematocheila) in a party in February 1996. The mul- lets were purchased at the Noryangjin fisheries market in Seoul. All of cases (2 males and 3 females) were 35 to 43 years old and healthy with the body weight range of 56-62 kg. They complained about gastrointestinal trouble and abdominal discomfort, but were in normal ranges of their hematology and urinalysis data. None revealed any sign of anemia The patients experienced natural discharge of a chain of segments before, and showed diphyllobothriid eggs in their stool specimens when they visited our laboratory. They were administered with praziquantel (15 mg/kg of body weight) and 30 g of magnesium sulfate as a purgative. Two whole worms with the scolices (310-340 cm in length; 8-13 mm in width) were expelled each from two out of five cases after anthelmintic treatment, and the others expelled the parts of strobilae without scolex. The worms were identified as D. Ictum, based on the following biological characters. external morphologies, coiling of uterus, the number of uterine loops, position of genital opening, morphologies of cirrus, cirrus sac and seminal vesicle on the histological sections, position of vagina and uterine pore , and microscopical and SEM morphologies of the eggs.

• Korean Journal of Microbiology
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• v.50 no.4
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• pp.360-367
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• 2014

Candida albicans is an opportunistic and the most prevalent fungal pathogen that can cause superficial and systemic infections in immunocompromised patients. C. albicans can promote the transition from budding yeast to filamentous form, generating biofilms. Infections associated with C. albicans biofilms are frequently resistant to conventional antifungal therapy. Therefore, the development of more effective antifungal drugs related with biofilm formation is required urgently. The roots of Rheum undulatum have been used for medicinal purposes in Korea and China traditionally. The aim of present study was to evaluate the effect of R. undulatum extract upon preformed biofilms of 12 clinical C. albicans isolates and the antifungal activities. Its effect on preformed biofilms was evaluated using XTT reduction assay, and metabolic activity of all tested strains was reduced significantly ($49.4{\pm}6.0%$) at 0.098 mg/ml R. undulatum. The R. undulatum extract blocked the adhesion of C. albicans biofilms to polystyrene surfaces, and damaged the cell membrane integrity of C. albicans which was analyzed by CFDA, AM, and propidium iodide double staining. It caused cell lysis which was observed by Confocal laser scanning and phase contrast microscope after propidium iodide and neutral red staining, respectively. Membrane permeability was changed as evidenced by crystal violet uptake. The data suggest that R. undulatum inhibits biofilm formation by C. albicans, which can be associated with the damage of the cell membrane integrity, the changes in the membrane permeability and the cell lysis of C. albicans.

• Parasites, Hosts and Diseases
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• v.23 no.1
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• pp.24-32
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• 1985

The worm development and egg laying pattern of Echinostoma hortense (Trematoda; Echinosto-matidae) were studied in albino rats and the brief clinical course was observed in human volunteers. A total of 21 rats were infected with 20~69 metacercariae each and two humans were with 7 and 27 metacercariae, which were collected from the loaches. For recovery of worms, the rats were sacrificed at irregular intervals from the 6th to 150th day after infection and the human volunteers were treated with praziquantel and purged with magnesium salt on the 26~27th day. The stools of the rats and humans were examined for the eggs. The results were as follows: 1. The worm recovery rate from the rats was not affected by the increase of infection time but varied individually; 9.1~50.0% (31.1 % in average). From humans, 14.3% and 37.0% (32.4% in average) of challenged were recovered. 2. In the rats, it was revealed that the worms rapidly grew for the first 14 days to become 7.59mm in average length and 1.17mm in average width but the growth became much slower thereafter until the 150th day; 7.95mm in length on the 21 th day, 9.04mm on the 28th day, 10.21mm on the 49th day and 12. 62mm on the 150th day. During the early stage of infection, the growth of genital organs (male or female) was expressed as sigmoid curves whereas non-genital organs (such as suckers) was simply as straight lines. 3. The prepatent period of this fluke was 10~12 days in the rats and 16~17 days in men. After the start of oviposition, the egg production by the worms remarkably increased, reached maximum on the 32~33th day, followed by decrease thereafter. The maximum value of E.P.G./worm was 390. 4. The major subjective symptoms in human volunteers were abdominal pain and diarrhea during the early stage of infection. The results show that human is as susceptible as the rats to E. hortense infection and the amount of egg production in the rats is greatly affected by the age of worms.

• Parasites, Hosts and Diseases
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• v.31 no.2
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• pp.99-108
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• 1993

An epidemiological study of Metogonimus infection was undertaken along the upper reaches of the Nmhn River, with special consideration on the species (type) of the worms collected from humans. Eggs of Metogonimus spp. were detected from 15 (9.7%) of 154 people examined in Umsong-gun, and from each Infected person (5 cases) 6.015- 24,060 worms (mean 13,2331 were recovered after treatment with praziquantel (10 mg/kg). Eggs were also detected from 37 (48.1% of 77 people In Yongwol-gun, from whom (27 casesl 1-4,965 worms (mean 1,2151 were collected. The worm from Umsong-gun consisted of both Metagonimus Miyata type and Metagonimus takohashii, whereas those from Yongwol-gun consisted of only Metqsonimus Miyata type. When the uterine eggs of the two kinds and M. yokogawoi (obtained from people In Tamjin River basin) were morphologically compared, it was suggested that the egg size should be a good Indicator for discrlmination of the species or type. The source of human Infection was proved to be fresh water fleshes: 49 of 52 Znsco plntvpus examined, 6 of 8 Hemibcrbus loniroskis, 13 of 15 Pseunogobio esuinl:, 4 of 6 Odontobutis obscura intemfptn, and 17 of 18 Corqssiw carassiw were found Infected with Metosonimw metacercariae. From the results, it is concluded that the upper reaches of the Namhan River are endemic foci of Metogonimn Miyata type and M. takahashii.