• Title/Summary/Keyword: 하순

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Biodegradation of Crude oil by Marine Bacterium Pseudomonas sp. CHCS-2 and Composition of the Biosurfactant (해양세균 Pseudomonas sp. CHCS-2에 의한 원유분해 및 생물유화제의 성분 분석)

  • 김학주;김봉조;하순득;황선희;공재열
    • KSBB Journal
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    • v.14 no.2
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    • pp.192-197
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    • 1999
  • marine bacterium Pseudomonas sp. CHCS-2 produced the biosurfactant in the culture broth which contained 2%(w/v) arabian light crude oil and the productivity of biosurfactant was increased with the addition of glucose. The crude oil in the culture broth was degraded by this strain and carbon chain of $_nC_{12}~_nC_{22}$ was completely degradaded during the incubation for 196 h. The crude biosurfactant was purified by Amberlite XAD-7, Sepharose CL-4B and DEAE-Sepharose CL-6B column chromatography. Therefore, 0.21g/L of the purified biosurfactnat was obtained. The purified biosurfactant was a type of lipoprotein and the molecular weight was estimated as 67kDa by SDS-PAGE. The lipid composition was identified as octadecanoic acid by gas chromatography/mass spectrometry. And then, the N-terminal amino acid sequence of the protein was determined as Ser-Val-lle-Asn-Thr-lle-X-Met-lle-Gly-Gln-Gln- and the sequence did not show homology to any other known lipoprotein. Therefore, the purified lopoprotein was predicted novel biosurfactant.

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Isolation and Identification of Marine Bacterium Cytophaga sp. AYK301 and Optimal Culture Conditions for the Production of Agarase (해양세균 Cytophaga sp. AYK301의 분리.동정 및 한천분해효소 생산을 위한 최적배양조건)

  • Lee, Won-Gyeong;Kim, Bong-Jo;Ha, Sun-Deuk;Gong, Jae-Yeol
    • KSBB Journal
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    • v.14 no.5
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    • pp.572-577
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    • 1999
  • A marine bacterium with highly effective agar degrading activity was ioslated from the southern sea of Korea (Chonnam, YoChon) and identified as Cytophaga sp. and named as Cytophaga sp. AYK301. This strain produced an extracellular agarase which had a high activity with agar. The optimum culture conditions for the production of agarase have been determined. For the increase of agarase productivity, 0.2% agar, 0.3% beef extract, and 0.05% NH$_4$NO$_3$ were used as carbon, organic and inorganic nitrogen source, respectively. The optimal initial pH, NaCl, culture time and temperature for the agar degrading activity were 7.5, 7.0%, 36 hr and $25^{\circ}C$, respectively. In the optimal conditions, the agarase production was increased up to more than 4.0 folds as compared to that by the basal medium.

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Studies on the Eradication of Pedicularis spp., Alternate Host of White Pine Blister Rust with Several Herbicides (제초제처리(除草劑処理)에 의(依)한 잣나무털녹병(病) 중간기주(中間奇主)(송이풀류(類)) 제거시험(除去試験))

  • Yi, Chang Keun;Kim, Hyun Joong;Yeo, Woon Hong
    • Journal of Korean Society of Forest Science
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    • v.51 no.1
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    • pp.51-55
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    • 1981
  • The study was conducted during 1975-1980 to evaluate the performance of several herbicides in controling alternate host, Pedicularis spp., of white pine blister rust disease in forest stands. The results obtained were summarized as follows. 1. It was found that Roundup was effective and most suitable herbicide in the fosest stands except that it accompanied some chemical injuries to neighboring plants. 2. As for the reduction density on the plot treated with Roundup was 100% and 65-91% respectively when investigated one and two years after its treatment on Pedicularis spp. 3. Another four chemicals, 2.4-D, Gramoxone, 2.4. 5-T and Para-col killed stems and leaves of Pedicularis spp. at the year treated, but appeared again next spring. 4. Eradication effectiveness in terms of spraying season was proved to be that late August was better than late May or June.

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Mixing Zone Analysis of Wastewater Effluent Discharged from Sokcho Ocean Outfall (속초 해양방류 하.폐수의 혼합구역에 대한 특성분석)

  • 강시환;박연숙;김상익;이호진
    • Journal of Korean Society of Coastal and Ocean Engineers
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    • v.13 no.3
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    • pp.245-253
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    • 2001
  • Mixing zone characteristics of the wastewater effluents discharged from Sokcho ocean outfall have been investigated using an outfall mixing zone model which was developed on the basis of Huang et al.'s(1996) analysis method. The model was applied to Sokcho ocean outfall case and was used to simulate the concentration distribution of wastewater effluents using winter season's data, ie. daily effluent flowrate, ocean current and density stratification data which were measure for two months in the outfall area. Hourly concentration distribution of outfall effluent discharges was calculated and they were averaged for the period of 15 days which covers the ambient flow variability of the neap and the spring tidal currents. The results show that near-field dilution was relatively high with the minimum dilution of 130 for the winter season. The mixing zone was extended to the coastal beach area rather than offshore because of major direction of coastal currents. This may cause a deteriorating impact on coastal water quality, especially to the adjacent swimming beach area.

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Seasonal Occurrence of Aphids and Selection of Insecticides for Controlling Aphids Transmitting Soybean Mosaic Virus (콩모자이크병 전염억제를 위한 진딧물 발생소장과 살충제 선발)

  • 김율호;노재환;김명기;임대준;허일봉
    • KOREAN JOURNAL OF CROP SCIENCE
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    • v.45 no.6
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    • pp.353-355
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    • 2000
  • The seasonal occurrence of aphids was investigated in the soybean Held to increase the control efficiency of aphid vectors of Soybean Mosaic Virus. The patterns of aphids occurrence were different according to planting time. There were two peaks in late June and mid-August in aphid population in optimum seeding (May 20), whereas the peak was around mid-June in early seeding(Apr, 20). Acyrthosiphon solani was dominant Species in early seeding, while Aphis glycines was dominant in optimum seeding. In early seeding, SMV incidence increased rapidly between 20 June and 30 June, suggesting that virus spread was strongly correlated with increased colonization of aphids. Imidacloprid WP, benfuracarb EC and acephate WP showed a good effect for the control of aphids without phytotoxicity. In the plot with infurrow treatment of imidachloprid G until 52 days after shooting, aphids were controled effectively. Acephate WP was applied as a foliar spray at V4, V6, or V4/V6 stage and all the treatments were effective on reducing: SMV incidence.

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'Choha', an Early-maturing and High-quality Apricot (식미 우수한 조생종 살구 '초하(初夏)')

  • Kang, Sang-Jo;Chung, Kyeong-Ho;Kim, Ho-Yeol;Jun, Ji-Hyae;Lee, Don-Kyun
    • Horticultural Science & Technology
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    • v.17 no.1
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    • pp.21-22
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    • 1999
  • 'Choha' was released by the National Horticultural Research Institute in 1997 as a new early-maturing and high-quality apricot variety, which originated from the crossing of 'N.Y. 472' and 'Early Orange' in 1986. This variety was tested as 'Wonkyo Sa-05' at 4 areas for 3 years from 1995. 'Choha' is resistant to fruit cracking by rainy spells just before fruit maturing. Fruits mature in late June, earlier than any other varieties. The content of soluble solids is $12.5^oBx$ and fruit acidity is low. Fruits are relatively large, about 60g in weight.

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Morphological Features of Coleosporium xanthoxyli and Its Alternate Host in Korea (산초나무 잎녹병균의 중간기주 및 형태학적 특징)

  • Lee, S.K.;Lee, K.H.;Lee, C.K.;Kim, D.Y.;Hwang, J.H.
    • Research in Plant Disease
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    • v.10 no.4
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    • pp.279-284
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    • 2004
  • A rust fungus has caused a serious early defoliation of Zanthoxylum schinifolium during growing seasons every year at the plantations located at Hadong and Jinju, Kyeongsangnam-Do in Korea. In order to identify the rust fungus and clarify its life cycle in Korea, aeciospores from Pinus thunbergii were artificially inoculated on the leaves of Z. schinifolium. Uredinial stage was successively formed on the leaves of Z. schinifolium. Based on the artificial inoculation test and on the morphological features of the dried specimens collected from P. thunbergii and Z. schinifolium, this rust fungus was identified as Coleosporium xanthoxyli. Morphological features of aecial and uredinial stages of the species were described. The first symptom of the infection was developed from later June to early July. And leaf infection ratio was 17.8%-58.7% during August at Hadong and Jinju regions of Kyeongsangnam-Do in Korea.

Application of Temporary Tongue-Lip Traction During the Initial Period of Mandibular Distraction in Pierre Robin Sequence (피에르로빈 연속증에서 골연장술의 초기에 일시적인 혀-하순 견인술의 적용)

  • Nam, Hyun Jae;Lee, Joon Ho;Kim, Yong Ha
    • Archives of Plastic Surgery
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    • v.35 no.3
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    • pp.349-353
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    • 2008
  • Purpose: There are 3 well-known surgical procedures to treat Pierre Robin sequence: tongue-lip adhesion, distraction osteogenesis of mandible, and tracheostomy. The classical tongue-lip adhesion is an effective way to keep airway. The tongue, however, becomes quite non-mobile and appears dormant until the patient could control upper airway and the adhesion can be maintained for prolonged period. Most of all, this procedure does not provide the correction of the micrognathia. Distraction osteogenesis is a good technique to correct micrognathia and to prevent tracheostomies in patients with Pierre Robin sequence. But airway keeping procedure is needed during the distraction period. The purpose of this study is to determine the usefulness of temporary tongue-lip traction during the initial period of mandibular distraction in Pierre Robin sequence patients with severe airway problems requiring operative procedure. Methods: It was a prospective study of 2 Pierre Robin sequence patients aged between 4 months and 6 months requiring surgical procedure to correct recurrent and severe pulmonary complications. Two patients underwent distraction osteogenesis of mandible. During the operation, deep one tension suture was performed to tract the tongue and lip. When the patient gained control of upper airway at the initial period of distraction and micrognathia was corrected, the traction suture was removed. Results: All patients were followed up. No patients complained severe pulmonary complications and tracheostomy could be avoided. No patients had severe pulmonary complication. The pulmonary condition of patients was good. Conclusion: In severe Pierre Robin sequence case, temporary tongue-lip traction is a good assistant method in distraction osteogenesis because this method can avoid tracheostomy.

Characteristics of Sap Exudation from Acer okamotoanum (Nakai) Plantation Forest in Jinju Region (진주지역 우산고로쇠나무 인공림의 수액 출수 특성)

  • Kim, Chang-Hwan;Park, Joon-hyung;Lee, Kwang-Soo;Park, Yong-Bae;Lee, Kyoung-Tae
    • Journal of the Korean Wood Science and Technology
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    • v.45 no.3
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    • pp.308-316
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    • 2017
  • This study was carried out to investigate the sap exudation characteristics in Acer okamotoanum (Nakai) plantation forest. As a result, Sap exudation quantity was rapidly reduced when mean temperature was increased by above $3.4^{\circ}C$. Considering this result, sap collection before end of February is more effective. Sap exudation quantity during the day started at approximately 9 o' clock, and reached the peak at approximately 11 o' clock, and ended at approximately 16 o' clock. Sugar content of sap was reduced with increasing period. Except for Na, and mineral components by tapping period were no significant difference.

Development of Upland Cultivation for Production of Marketable Rhizomes in Wasabia japonica Matsum. (고추냉이 밭재배에서 근경의 상품성 향상을 위한 재배체계)

  • Moon, Jung-Seob;Jang, Young-Gik;Choi, Dong-Chil;Choi, Joung-Sik;Kim, Hyung-Moo;Choi, Yeong-Geun
    • Korean Journal of Medicinal Crop Science
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    • v.12 no.6
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    • pp.473-477
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    • 2004
  • Wasabis (Wasabia japonica Matsum.) have been used as a spice with its petioles and rhizomes, but the production of its rhizomes in upland cultivation was very difficult for the severe damage of rotting disease during summer season. This experiment was carried out to increase the yield of marketable rhizome in wasabi when cultured in upland. Seedlings were raised in sub-alpine area for 7 and 12 months and then transplanted to experimental area on Sept. 20 and the yield was investigated on May 12 of the next year. The marketable rhizome(above 40g/plant) was produced in seedlings raised for 7 months. Production rate of marketable rhizome was 13.3% and total yield was 72.9 kg/10a. The content of allylisothiocyanate in rhizome of upland wasabi was 0.777 mg/g and its content was equal to wasabi cultured in water condition for 18 months. These results suggested that the upland cultivation of wasabi seedlings raised in sub-alpine area for 7 months was possible to product marketable rhizome.