• Journal of the Korean Society of Food Science and Nutrition
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• v.39 no.8
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• pp.1213-1219
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• 2010

We evaluated the anti-aging potential and safety of black garlic extract for cosmeceutical ingredient. Black garlic was made by spontaneous fermentation for 40 days at $60{\sim}70^{\circ}C$, 85~95% RH without any additives. The 10% black garlic extract had sweet odor, antioxidant activities and inhibitory activities of skin againg enzymes such as tyrosinase and elastase. The skin safety was performed to evaluate of potential toxicity using the primary irritation test and skin sensitization test. The black garlic extract did not show any adverse reactions such as erythema and edema on intact skin sites at primary irritation test, but on abraded sites, some experimental animals showed very slight erythema. So, the black garlic extract was classified as a practically non-irritating material based on the score 0.23 of primary irritation index. The skin sensitization study was tested by the guinea pig maximization test (GPMT) and Freund's complete adjuvant (FCA) with intradermal injection of 10% black garlic extract. The skin sensitization test showed no skin sensitization. The allergic sensitization depends on tumor necrosis factor-$\alpha$ (TNF-$\alpha$) and interleukin-6 (IL-6). The concentration of IL-6 on challenged tissue of treated with black garlic extract was not significantly different with negative control group (saline treated group). Based on this study, the potential for black garlic as a cosmeceutical ingredient was proven.

• Journal of Food Hygiene and Safety
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• v.36 no.1
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• pp.17-23
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• 2021

This evaluation tested the skin irritation and sensitization of an acaricide (Wagoojabi II®, WGJB) for the control of poultry red mite, containing 20% Chamaecyparis obtusa oil and 56% Cinnamomum camphora oil. In a primary skin irritation test, rabbits were dermally treated with WGJB for 24 h. The acaricide did not induce any adverse reactions such as erythema and edema on intact skin sites, but on abraded skin sites, some rabbits showed very slight erythema and edema 24 h after topical application. So, the acaricide was classified as a practically mild-irritating material based on a 0.625 primary irritation index score. In the skin sensitization test, guinea pigs were sensitized with intradermal injection of 0.1mL WGJB for 24 h. After 1 week, The WGJB was treated on the site of injection, and challenged 2 weeks later. The WGJB did not induce any allergic reactions. Therefore, the WGJB was graded as a weak material at '0' in both sensitization score and rate. From the results of this study, it is suggested that WGJB does not cause contact irritation and sensitization.

• Journal of Adhesion and Interface
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• v.21 no.4
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• pp.162-167
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• 2020

The skin-inspired multimodal soft sensors have been developed through multidisciplinary approaches to mimic the sensing ability with high sensitivity and mechanical durability of human skin. For practical application, although the stimulus discriminability against a complex stimulus composed of various mechanical and thermal stimuli experienced in daily life is essential, it still shows a low level actually. In this paper, we first introduce the operating mechanisms and representative studies of the unimodal soft sensor, and then discuss the recent research trend in the multimodal soft sensors and the stimulus discriminability.

• Korean Chemical Engineering Research
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• v.46 no.2
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• pp.227-232
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• 2008

In this research, organic extracts from roots of platycodon grandiflorum was tested to see the possibility for cosmetic natural surfactant. Interfacial properties of extracts of Platycodon grandiflorum was checked for interfacial tension, forming force, dispersion force, emulsion force, emulsion activity, and emulsion stability. At 0.005 wt% concentration, the interfacial tension against the caster oil was 11.5 dyn/cm which was lower than that of Tween 40 and quillaja bark. Extracts of Platycodon grandiflorum showed excellent emulsification activity and stability for cosmetic oils such as olive oil, soybean oil, and canola oil. In patch test using 2-5% of the extract and glycerin, the extract showed mild skin irritation. From the experiment, the extracts of platycodon grandiflorum root showed good interfacial properties as a cosmetic agent with minor skin irritation.

• Journal of the Korean Applied Science and Technology
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• v.40 no.3
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• pp.453-461
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• 2023

This study was to verify the skin safety of Coptis Japonica extract(CJE) -containing cosmetics by patching them on sensitive skin and checking its stimulating level. 32 subjects diagnosed with sensitive skin by the result of the lactic acid injury test, were subjected to 20 ㎕ of solubilized essence mixed with 2% CJE on the test subjects' back and closed patched for 24 hours. No stimulus reactions were found by bare eyes and scored 0 stimulus figure points after they were taken off in 30 minutes, 24 hours, and 48 hours. Coptis japonica's medical efficacies such as antioxidant, and anti-inflammatory are expectable to be used as functional cosmetics, and this research would be the foundation of further research into its cosmetical usage.

• Journal of Conservation Science
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• v.33 no.4
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• pp.255-266
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• 2017

Chemically derived pesticides have been used to prevent biological damage to domestic cultural property. However, their use is gradually being restricted due to the harmful effects on the human body and environment. Therefore, there is a growing interest in the search for new antifungal biopharmaceuticals whose safety has been confirmed by toxicity evaluation through animal experiments. This paper presents methods of toxicity evaluation of natural biocides using Sprague-Dawley rats and New Zealand White (NZW) rabbits. Safety of the natural biocide extract of Asarum sieboldii was evaluated using single-dose oral and dermal toxicity tests in Sprague-Dawley rats, and eye and skin irritation tests in NZW rabbits. The extract has proven antimicrobial and insecticidal activities against wood-rotting fungi and termites. After single oral administration to rats, the $LD_{50}$ values were determined to be over 4,000 and 2,000 mg/kg for males and females, respectively. After single dermal administration to rats, the $LD_{50}$ values exceeded 10,000 mg/kg for both males and females. The extract was identified to be non-irritant to the rabbit eye, and only slightly irritant to the rabbit skin. In this study, we confirmed the safety of the A sieboldii extract through animal testing. Due to the harmfulness of humidifier disinfectants, focus is on the safety of chemical pesticides, and toxicity evaluation is suggested as the basic method for hazard evaluation.

• The Journal of Korean Medicine Ophthalmology and Otolaryngology and Dermatology
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• v.31 no.2
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• pp.11-23
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• 2018

목적 : 본 연구에서는 $TNF-{\alpha}$와 $IFN-{\gamma}$로 자극한 인간피부각질형성세포 (HaCaT keratinocytes) 모델을 사용하여 지모가 피부장벽 기능에 미치는 영향을 알아보고자 하였다. 방법 : MTT assay를 통하여 지모 주정(70% 에탄올) 추출물 (EAA)이 HaCaT keratinocytes의 세포생존율에 미치는 영향을 확인하였으며 wound healing assay를 통해 EAA가 HaCaT 세포의 이주 능력에 영향을 주는지 관찰하였다. 또한 western blot analysis와 qRT-PCR을 통하여 EAA가 $TNF-{\alpha}/IFN-{\gamma}$로 자극한 HaCaT 세포에서 iNOS의 단백질 발현 및 IL-4, IL-13, IL-6의 mRNA 발현, filaggrin의 단백질과 mRNA 발현에 미치는 영향을 조사하였다. 결과 : EAA는 처리 농도 $500{\mu}g/ml$까지 HaCaT keratinocytes의 세포생존율에 영향을 미치지 않았다. EAA는 wound healing assay에서 HaCaT 세포의 이주 능력을 증가시켰으며, $TNF-{\alpha}/IFN-{\gamma}$로 자극한 HaCaT 세포에서 iNOS의 단백질 수준을 감소시켰다. 또한 EAA가 IL-4, IL-13, IL-6의 mRNA 발현을 억제하는 것 역시 확인할 수 있었다. 뿐만 아니라 EAA는 $TNF-{\alpha}/IFN-{\gamma}$ 자극에 의해 감소했던 filaggrin을 단백질과 mRNA 수준에서 회복시켰다. 결론 : EAA가 HaCaT 세포에서 Th2 type cytokines, pro-inflammatory cytokine의 억제와 filaggrin 회복을 통해 피부장벽 기능 손상에 대한 억제활성을 갖는 것을 확인하였으며, 이를 통해 EAA가 염증으로 인해 손상된 피부장벽 기능 개선에 효과적일 것으로 사료된다.

• Proceedings of the Korea Air Pollution Research Association Conference
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• 2000.11a
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• pp.348-349
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• 2000

HF는 주로 음료수와 식수에 의해 폭로되며, 작업자의 경우는 공장 작업과정에서 공기 흡입과 피부 접촉으로 폭로된다. 단기적인 흡입에 의한 폭로는 폐수종 및 자극 등을 주며, 눈과 피부에 직접 폭로시에는 화상과 자극을 준다. 장기간의 폭로는 주로 음료수와 음식에 의해 폭로되며, 공기에 의한 폭로는 코, 인후 및 기관지에 충혈 및 자극을 주고(U. S. EPA, 1989; U. S. EPA, 1993), 식물의 경우에는 잎의 끝이나 가장자리의 변색, 발육 부진 및 병에 대한 저항성 약화 등에 영향을 미치는 것으로 알려져 있다(Cao, 1998). (중략)

• 월간산업보건
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• s.291
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• pp.12-16
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• 2012

과황산암모늄 (ammonium persulfate), 과황산칼륨(potassium persulfate), 과황산나트륨(sodium persulfate)에 대한 작업 노출기준은 perfulfate ($S_2O_8$) 형태로서 0.1 $mg/m^3$(TLV-TWA)으로 권고하였다. 이 기준치는 피부 자극 및 피부염을 포함한 피부 또는 기관지계 관련 질환 발생을 최소화하기 위해 설정되었다. 몇몇 연구 자료에 의하면 이 물질들에 노출되면 폐 자극 증세도 나타난다고 보고되고 있다. 또한 이 TLV는 전신독성 측면보다는 자극 영향 측면에 근거를 두어 제안하였다. "감작제(SEN)", 발암성, TLV-STEL에 대한 설정은 유용한 자료의 부족으로 아직 권고하지 않고 있다.

• The Journal of the Korea Contents Association
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• v.19 no.10
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• pp.198-205
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• 2019

The purpose of this study was to identify whether cutaneous sensory (CS) changes induced by mechanical intervention(MI) increases the trigger point threshold and muscle tone of the same spinal segment to neck disc patients. Thirty persons with Neck disc patients were recruited in this experiment. The subjects consisted of 10 men and 20 women. The mechanical stimulus group induced CS changes for 5 minutes using the Graston instrument and the control group received no action. The CS changes were estimated by using the Von Frey Filament, PPT changes were measured by using the pressure threshold meter and msucle tone changes were measured by using Myotone pro. CS threshold increased significantly when MI was applied (p<0.05). On the same spinal segment, increases in the right infraspinatus PPT and muscle tone was observed (p<0.05) and decreases in the right trapezius PPT was observed(p<0.05). However, the PPT and muscle tone changes in other muscles were not significantly different. Furthermore, the control group CS, PPT and muscle tone were not significantly different. As a result, CS changes induced by MI make to change PPT and muscle tone on the same spinal segment. Therefore, application of MI to the same spinal segment may be of clinical significance as a new rehabilitation method for increasing pain threshold, muscle tone and pain control in neck disc patients.