• Journal of Embryo Transfer
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• v.18 no.3
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• pp.237-242
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• 2003

These studies were carried out to establish an effective in vitro embryo transfer methods by analyzing several factors. The base media were TCM-199 solution for in vitro maturation(IVM) of bovine follicular oocytes and Fer-TALP solution for in vitro fertilization(IVF) and CRlaa medium for in vitro culture(IVC). IVC used the fertilized oocytes of 24-hr culture (day 1)after IVF. Embryos were cultured in drop-culture that contained 25 embryos per 10${mu}ell$. Blastocysts cultured for 7 to 9 in vitro were transferred to recipients. The results obtained were as follows: 1. The pregnancy rate according to different region of embryo transfer were 33.8％, 48.1％, 45.0％ and 35.3％ respectively. 2. The pregnancy rate according to the parity of recipient when embryos were transferred to nulliparous (42.9％) was higher than that of 1∼3nd parlous(36.9％), however there were not show significant difference each other. 3. According to the stage of blastocyst, the pregnancy rate when middle blastocysts (MB) (45.5％) were transferred to recipients were higher than that of late blastocysts (LB) (41.0％). 4. When IVF-derived blastocysts cultured for 7 to 9 day were transferred to recipients, the pregnancy rate was higher 7 day of blastocyst than that of 8 day or 9 day of blastocyst. The results of embryo transfer according to the regions, the parity of recipient and the development stage showed that blastocyst formed for 7day transferred to nulliparou were higher pregnancy rate than others.

• Korean Journal of Animal Reproduction
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• v.26 no.2
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• pp.173-182
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• 2002

The mechanisms underlying of the visual assessment and resulting in optimum embryonic development following in vitro maturation, fertilization, and culture are unclear, It was known that in vitro produced embryos show more frequent occurrence of fragmentation, which resulted in poor developmental potential and decreased implantation rate. The objective of this study was to investigate the apoptotic rates in bovine blastocyst derived from in vitro fertilization (IVF) and nuclear transfer (NT). In addition, the expression levels of Bcl-2 and Bax gene were investigated in the blastocyst to confirm their potential roles in the regulation of apoptosis during preimplantation embryonic development. Analysis of apoptosis was carried out by using terminal deoxynucleotidyl transferase mediate dUTP nick end labeling (TUNEL) method. The levels of Bcl-2 and Bax gene in the blastocyst derived from IVF and NT were determined by RT-PCR. The proportion of TUNEL positive signal in blastocyst derived from NT was significantly higher than that in blastocyst derived from IVF (p<0.001). Bcl-2 expression level of blastocyst derived from IVF was higher than that of blstocyst derived from NT. However, high expression level of Bax was observed in the blastocyst derived from NT. These results indicates that apoptosis is more responsible for fiagmentation in bovine blastocyst derived from NT than IVF. These results suggested that the increase of developmental failure followed by NT could be caused by nuclear fragmentation as apoptosis.

• Journal of Embryo Transfer
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• v.13 no.3
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• pp.251-259
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• 1998

Concentrations of free amino acids in the BF of IVP bovine BL and HBL were examined in this study. The embryos derived from IVF oocytes were cultured in a SOFM containing BSA, EAA and NEAA. BF was aspirated from BL (180 h of age after insemination) and HBL (216 h of age after insemination), and introduced into drops of SOFM (30$\mu$l/drop) containing PVA through micromanipulation. The medium containing BF was then subjected to measurement of 20 amino acids by an automatic amino acid analyzer. The concentrations of isoleucine, leucine and methionine were higher (p〈0.05) in the BF from HBL than from BL, and no difference was found in aspartate or glutamate concentrations between BL and HBL, while threonine, alanine (p〈0.01) and the rest of the amino acids (p〈0.001) were significantly higher in the BF from HBL than from BL. Cystine was not found in either BL or HBL. A high concentration of glutamine was found in the BF from both BL and HBL, although it was not added to the culture medium. These results indicate that bovine BF contains several EAA (methionine in BL and isoleucine, leucine and methionine in HBL) and NEAA (alanine, glutamate, glycine, proline, serine and aspartate in BL, and glutamate and aspartate in HBL), and there is significant differences in the amino acid concentration in the BF between BL and HBL derived by WP.

• Journal of the Korean Society of Food Culture
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• v.7 no.3
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• pp.237-244
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• 1992

A total of 9 different names of dried beef were found in the literatures. They were classified in to 4 groups according to their processing method and shape: San pc(about 1cm thick beef salted and dried), Pyun po(ground beef with the seasoning, make small size or large size, dried), Yak po(thin beef or ground beef in the seasoning, dried), Jang po(thin beef in the seasoning, dried until half, again in the seasoning, dried). Most of the po was prepared with beef but phesant was also used for preparation Po in salt, seasame oil, black powder and soy sauce amomg the 19 kinds of seasoning, and chopped pinenut among 3 kinds of decorating were commonly used.

• Journal of Energy Engineering
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• v.7 no.1
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• pp.57-64
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• 1998

A neutralized fabric filter of which major raw materials were polyester and stainless steel fibers was developed and its physiochemical properties and basic filter characteristics were investigated. Four finds of dusts generated in the typical domestic industry were used, which were coke dust from a steel manufacturing process, cement dust from a cement manufacturing process, flu ash from a fluidized-bed combustor, and incinerator ash from a waste plastics incinerator. The physicochemical properties of the neutralized fabric filter were analyzed in terms of changes in tensile strength and initial elastic modulus under $SO_2$ and $NO_2$ atmospheres, mean flow pore pressure, bubble point pore diameter, mean flow pore diameter, and pore size distribution. In addition, the pressure drop, dust penetration, and figure of merit for the fabric filter were investigated in a bench-scale filter testing unit. The pressure drop increased as the filtration velocity and dust loading increased, and its increasing shape depended on the type of dust. The dust penetration rapidly decreased as the dust loading increased irrespective of the type of dust. The figures of merit for the fabric filters increased in the early stage of filtration and then showed rapid decreases followed maintaining a constant level.

• Journal of Korea Multimedia Society
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• v.14 no.8
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• pp.1070-1081
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• 2011

Among visual communications methods through new media, the typography becomes a key role in information transfer areas with the development of motion graphics. Recently, the researches on stereoscopic image productions have been carried out actively to increase the reality of created images, and the various new approaches for the production of stereoscopic images have been investigated. However, it is obvious that the researches using motion typography have not been studied sufficiently. Thus in this study, the stereoscopic images for moving typography are suggested and produced by using three experimental studies -- the experiments using text movement, camera movement, and finally, editing and composition. In the experiments, from the stereoscopic motion typography produced by our proposed methods, the various visual effects have been obtained, and the visual communication with high reality has been achieved.

• The Korean Journal of Zoology
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• v.33 no.1
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• pp.12-21
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• 1990

When a piece of the dorsal skin(cat fish) was observed under a light microscope, melanophores were horizontally expanded and had a few processes filled with melanosomes. They were isolated from one another. Some melanophores looked black, while others were d&k brown. On observation with a transmission electron microscope, the epidermal melanophore of cat fish was highly branched and small segments of processes were found frequendy m the vidnity of the interecellular spaces. The cross section of the processes of melanophore was almost circular, and often invested by a thin layer of epidermal cells. Some processes, however, occurred free m the wide interecellular spaces or at the cytoplasm of the superilcial layers. In the mature melanophore, the cell organelles including melanosomes, mitochondria and free ribosomes were prominent in the perinuclear portions. Many melanosomes were spherical or elipsoidal in shape. Each melanosomes was surrounded by a limiting membrane. The processes of the mature melanophore were well developed, but the processes of the immature melanophores were incompletely developed.

• Archives of design research
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• v.15 no.3
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• pp.259-270
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• 2002

In 1995, When people read a poem, the image that a poet intends to convey to readers shows in various colors according to the status of their emotion. Poetry is a bridge as well as a text, which connects this world and the poet's world. In such relationship, the communication through Types occurs. The realm of application of modern typography is widening due to the development of the Internet and mass media, and the ways of expression of which are changing with the help of lots of softwares. So, the modern typography is re-born as an organic language which is alive, breathing. Therefore, Types has the structural character similar to that of Typography, which is a language of image, creating today's movement, time, and space. The already existing poetry contains meanings but has a descriptive structures. On contrary, compared with the former, the type appeared in Hangul Concrete Poetry., itself is a poem in another realm due to the formality native to Hangul, and which appears in non-linear structure. So, in this thesis, I will analyze the formality and non-linear structure of Hangul Typography in order to widen the realm of research on typography, which is a very meaningful trial to visualize the literature.

• Proceedings of the Korea Water Resources Association Conference
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• 2012.05a
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• pp.440-440
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• 2012

본 연구에서는 수질관리 및 기후변화(온실가스 저감) 등에 부응할 수 있는 SRI 벼재배 방법을 국내 논에 적용하여 농업비점오염원과 온실가스 저감효과를 측정하고 비교하여 SRI의 환경개선효과를 평가하고자 하였다. 시험포는 대조구인 상시담수처리구(관행, 재식거리 $30{\times}15cm$)와 SRI 물관리 처리구로 조성하였다. 각 시험포에는 관개배수시설 및 관개량을 측정할 수 있는 수도계량기, 유출량 측정을 위한 플륨 및 수위계 그리고 온실가스(메탄 및 이산화질소)를 측정하기 위한 아크릴재질의 Chamber를 설치하였다. 관행 및 SRI 시험포에 이앙할 모의 재배품종으로 오대벼를 공시하고 모든 시험포의 경우 1주당 3-5본씩 기계이앙을 실시하였으며, 물관리를 제외한 시비와 제초 등의 영농관리는 동일하게 수행하였다. 메탄($CH_4$)과 아산화질소($N_2O$)는 주 2회, 오전 9시 12시에 60 mL 주사기로 주기적으로 시료를 채취하여 GC로 분석하였다. 그리고 관개기간동안 관개량, 강우량 그리고 강우 유출량을 측정하고 수질시료를 채취하여 오염부하를 산정하였다. SRI 시험포의 SS, $COD_{Cr}$, $COD_{Mn}$, BOD, TN, TP의 총 오염부하량은 각각 583 kg/ha, 210.8 kg/ha, 70.1 kg/ha, 30.7 kg/ha, 56.1 kg/ha, 3.55 kg/ha로서 관행 시험포의 오염부하량에 비해 27.1~46.0%의 오염물질 저감 효과를 보였다. 그리고 각 시험포별 온실가스 메탄과 아산화질소의 총 배출량을 지구온난화잠재력(GWP)으로 환산하여 이산화탄소($CO_2$) 기준으로 산정한 결과, 관행은 14.2 톤/ha 그리고 SRI 물관리 처리구 4.0 톤/ha로 관행 대비 SRI 처리구에서 71.8%의 온실가스 감축효과를 나타내었다. 따라서 SRI 벼재배기술은 논 비점오염부하 및 온실가스 저감을 위한 효과적인 최적영농관리방법인 것으로 판단된다.

• Journal of Game and Entertainment
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• v.2 no.2
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• pp.61-69
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• 2006

Along with advance in visual culture, the importance of motion graphic has been increasingly emphasized day by day, which can maximize information delivery using image illustration and typography, graphic factors of images. In addition, we can easily see increasing cases where what a designer intends is visualized using creative typography in diverse mass media such as TV commercials, movies or web. Thanks to the effects of this trend, various ways of manufacturing works have been proposed in the field of motion typography by applying diverse factors including verbal ones, time, form, motion, colors, and sound for the purpose of expressing formless semantic notions through visual form of typography. However, physiological features such as sex, age, health status, pathological conditions, and body size can have a bigger effect on the process of real communication. Therefore, if property of quasi-verbal sound can be reflected appropriately in motion typography where communication is expressed only by visual form, it can enable people to understand what a designer intends faster and more exactly.