• Journal of Plant Biotechnology
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• v.42 no.4
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• pp.356-363
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• 2015

In order to study genetic engineering in trees, the characterization of genes and promoters from trees is necessary. We isolated the promoter region (867 bp) of Pagns-LTP from poplar (P. alba ${\times}$ P. glandulosa) and characterized its activity in transgenic poplar plants using a ${\beta}$-glucuronidase (GUS) reporter gene. High-level expression of the Pagns-LTP transcript was found in poplar roots, while comparatively low-level expression was found in the young leaves. Pagns-LTP mRNA was not detected in other poplar tissues. Additionally, transgenic poplar plants that contained a Pagns-LTP promoter fused to a GUS reporter gene, displayed tissue-specific GUS enzyme activity localized in root tissue. In silico analysis of the Pagns-LTP promoter sequence reveals the presence of several cis-regulatory elements responsive to phytohormones, biotic and abiotic stresses, as well as those regulating tissue-specific expression. These results demonstrate that the Pagns-LTP promoter has tissue-specific expression activity in poplar roots and leaves that may be involved in organ development and plant resistance to various stresses. Therefore, we anticipate that the Pagns-LTP promoter would be a useful tool to genetically optimize woody plants for functional genomics.

• Journal of Korean Society of Forest Science
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• v.86 no.1
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• pp.80-86
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• 1997

The objective of this study was to investigate how resistant poplar hybrid makes compensation to ozone stress. Growth, net assimilation rate and initial Rubisco activity were investigated. This study elucidates the physiological mechanisms associated with ozone sensitivity and resistance in 3 selected $F_2$ hybrids, a family originating from a cross between Populus trichocarpa${\times}$P. deltoides. Open-top chambers were used. Ozone concentrations varied from 90 to 115 ppb for 126 days, 6 to 9 hours in a day. This study tested the hypothesis that resistant poplar hybrid maintains the biomass production to ozone exposure via increased net assimilation rate and Rubisco activity. Growth, biomass, net assimilation rate and initial Rubisco activity were generally reduced by ozone treatment. In the tree parts, root under ozone stress was the most sensitive part. Reduced allocation of photosynthates to root growth might be due to increased respiratory demands for maintenance and repair of aboveground tissue damaged by ozone stress. Maintenance or increases remaining leaves in photosynthetic rates and Rubisco activity in resistant clone in response to ozone treatment were the results of biological compensation to ozone stress.

• Journal of agriculture & life science
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• v.44 no.3
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• pp.15-21
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• 2010

We estimated the biomass productivity and the storage potential of nitrogen, the major contributor of non-point source pollution, with four three-year-old four poplar clones in a riparian woody buffer established in the Anseong River in Anseong, Korea. Stem of Populus alba ${\times}$ P. glandulosa clone 72-31 and Populus deltoides ${\times}$ P. nigra clone Dorskamp showed the highest percentage of aboveground biomass components, followed by branch and leaf. Nitrogen content in aboveground biomass components of two poplar clones was the highest in leaf and the lowest in stem. Nitrogen content in leaf and branch of clone 72-31 was higher than that of clone Dorskamp, while it in stem was lower. Populus deltoides clone Ay48 showed the highest above-ground biomass productivity, which was estimated as $37.5ton\;ha^{-1}$ at age 3. However, clone 72-31 was the lowest in above-ground biomass productivity. Nitrogen storage potential in aboveground biomass of 3-year-old poplar clones was high in order of aboveground biomass. Clone Ay48 showed the highest nitrogen storage potential in aboveground biomass, which was estimated as $218.3kg\;ha^{-1}$ at age 3.

• Journal of agriculture & life science
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• v.45 no.5
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• pp.17-23
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• 2011

Growth characteristics and adaptability of 10 poplar clones planted at a reclaimed tidal flat were evaluated. The contents of $Na^+$, $Ca^{2+}$ and $Mg^{2+}$ were 10.0, 3.4 and 1.5 times higher, respectively than those of control although the electrical conductivity(EC) in the soil at the test plantation was low as much as 0.51 dS/m. The contents of organic matter(OM) and total nitrogen(TN) in the soil were 22.9 and 23.0 times lower than those of control. Average survival rate of 10 poplar clones showed 88% at three years after planting. Clones Eco28(Populus euramericana), Dorskamp(Populus deltoides ${\times}$ P. nigra) and I-476(Populus euramericana) showed the best survival rate of 100%. However, clones 97-19(Populus deltoides(Lux) ${\times}$ P. deltoides(Harvard)) and Suwon (Populus koreana ${\times}$ P. nigra var. italica) were relatively lower than other clones. Average height and DBH of all clones were 4.8 m and 3.6 cm, respectively. Clone Dorskamp showed the greatest height and DBH, 5.9 m and 5.0 cm, respectively. Clones 97-19 and Dorskamp showed the least defoliation by stress and visible damage by insects and diseases, whereas clones Suwon and I-476 were the most sensitive at the reclaimed tidal flat. Clone Dorskamp showed the best adaptability at the reclaimed tidal flat, but clone Suwon showed the worst based on survival rate, growth, and visible damages.

• Journal of Mushroom
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• v.16 no.4
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• pp.338-341
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• 2018

This study investigated the possibility of using pine tree sawdust in thinning-out tree as a substitute for poplar and douglas-fir tree sawdust in oyster mushroom cultivation. Mycelial growth was 10.8 cm in medium supplemented with 70% pine sawdust after 18 days of culture and 10.2 cm in control medium. Mycelial density showed high density with no significant difference between treatments. Fresh weight and diameter of fruiting bodies were slightly lower in medium supplemented with pine tree sawdust. The hardness of pine tree sawdust was slightly higher in the medium supplemented with 20% and 30% of pine tree sawdust. The L value showed a tendency to be higher in the pine added medium, but the a and b values d id not show any significant difference between the treatments. The yields of fruiting body were 157 g / 850 ml for the control and 170 g / 850 ml for the 40% added pine tree sawdust.

• Journal of Mushroom
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• v.19 no.3
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• pp.160-165
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• 2021

This study was conducted to investigate a suitable substrate formulation for bag cultivation of Hypsizygus marmoreus. To determine the optimum media composition for H. marmoreus, chemical properties of various media and culture properties were investigated. The fastest primordia formation (9.0 days), the shortest fruiting body harvest period (18.0 days), and the highest yield (144.7 g/bag) were realized with the T6 formulation (55:15:20:5:5 mixing ratio of poplar sawdust, wheat bran, rice bran, dried soybean powder, and palm kernel, respectively). The chemical properties of the T6 formulation were pH, 6.0; total carbon, 19.0%; total nitrogen, 0.83%; C/N ratio, 22.8; P₂O₅, 0.61%; K₂O, 0.46%; and MgO, 0.29%. This is the first attempt using plastic bags for cultivation and will help expand the production of H. marmoreus.

• Korean Journal of Agricultural and Forest Meteorology
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• v.5 no.1
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• pp.36-42
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• 2003

To identify the rapacity of waste leachate absorption in Populus euramericana and Betula platyphylla var, japonica, four different treatments were applied to seedlings: leachate solution (100% leachate), 50% dilution (50% leachate: 50% water, v/v) and 25% dilution, (25% leachate: 75% water, v/v) were applied to these two species. After the experiment, concentrations of heavy metals in tree parts were analyzed by an Inductively Coupled Plasma emission spectrometer (ICP). treatment with waste leachate significantly stimulated both Populus euramericana and Betula platyphylla var. japonica biomass production relative to the water control. In addition, these species showed good absorption capacity of As, Co, Hg and Ni elements. The results of this study suggested that these two species can absorb the toxic materials through their roots and transport them to stems or leaves.

• Journal of Korean Society of Forest Science
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• v.94 no.4 s.161
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• pp.236-242
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• 2005

Phytochrome B (PhyB) gene, which is a photoreceptor that controls plant growth under various light conditions, was cloned from Chinese hybrid poplar 'Soohang 1'. Nucleotide sequence and deduced amino acid sequences PhyB cDNA of 'Soohang' is consisted with 3,456 nucleotides and 1,156 amino acids. The cloned PhyB fragment showed 98% homology of amino acid sequences with Populus balsamifera PhyB1. According to Northern blot analysis. PhyB was up-regulated by light, while PhyB transcript was not detected under dark condition. According to this study, the cloned PhyB is induced by light and functions as photoreceptor.

• Journal of Mushroom
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• v.11 no.3
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• pp.145-148
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• 2013

Studies were made to optimize the media composition and cultural condition for mycelial growth of ear mushroom(Auricularia auricula). Sawdust spawn of media composition for optimal growth were found to be quercus sawdust combination of 20% rice bran and poplar sawdust combination of 10% wheat bran were the best of the optimal combination. The optimal condition of the most suitable inoculation amount showed that quercus spawn and poplar spawn were 15g, 25g, respectively.

• Journal of Korean Society of Forest Science
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• v.95 no.6
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• pp.743-750
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• 2006

Poplars and willow were planted to identify suitable species and varieties for landfill reclamation at the Sudokwon Landfill Site in 1997. Survival rate, growth performance, vitality, visible foliar injury by pollutants, fungi, and leaf insects, and stem borer damage have been investigated for 10 clones of 4 poplar species and 2 clones of one willow species from 1997 to 2005. The average survival rates of poplar and willow clones were drastically decreased from 90% in 1997 to 53% in 2005. Among poplar species, Populus alba ${\times}$ P. glandulosa showed the highest mean survival rate of 66%, while Populus koreana ${\times}$ P. nigra var. italica and Populus euramericana were the lowest of 41%, respectively in 2005. Clivus, which is one of the clones from Populus alba ${\times}$ P. glandulosa, showed the highest survival rate of 73%. For mean height, Ec028 clone(P. euramericana) showed the highest of $11.2m{\pm}2.1m$ and followed by Clivus of $11.0m{\pm}2.0m$. Clone 131-27(Salix alba) was the lowest of $7.8m{\pm}1.6m$. Vitality, defoliation, visible foliar damage, and stem borer damage were significantly different among clones. Most of Populus alba ${\times}$ P. glandulosa and Salix alba clones seemed to have strong vitality and to be tolerant to various stresses at the site. However, Populus nigra ${\times}$ P. maximowiczii was sensitive to the stress. We have selected 5 clones in total: Clivus as the best clone for waste landfill reclamation, and additionally two Salix clones 131-25, 131-27 and two clones of Populus alba ${\times}$ P. glandulosa (72-9, 72-16) have been selected. These five clones could be supplied for planting at sites having an environment similar to the Sudokwon Landfill Site.