• Title/Summary/Keyword: 포자

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Effects of Light on Reproduction of Gibberella zeae and Overwintering of Soil-Borne Conidia (밀붉은곰팡이병균의 분생포자 및 자낭각 형성에 미치는 광선의 영향 및 토양에서의 분생포자월동)

  • Kim Hee Kyu;Chung Hoo Sup
    • Korean journal of applied entomology
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    • v.11 no.1
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    • pp.31-35
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    • 1972
  • 1. Continuous light induced more conidia than alternating light and darkness treatment in isolate Chinju 1. Isolate Suwon 3 produced much more conidia on synthetic medium than Chinju 1 in light. Conidial formation in Suwon 3 increased remarkably with alternate light and darkness in 8 days incubation. 2. Light was essential for perithecial formation in Chinju 1. No matured perithecia were observed in Suwon 3 with any treatment. Abundant perithecia were produced in Chinju 1 but only perithecial initiation occurred in Suwon 3. Suwon 3 produced significantly more conidia than Chinju 1, while perithecial formation was reversed. 3. Conidial numbers in soil decreased significantly through the winter at 10, 30 and 50 per cent soil moisture, with the most striking decrease at 10 per cent levels, but the number recovered again beginning in March, regardless of the soil moisture. The above results provide us a useful clue to support the possbility the conidia may serve as a primary inoculum.

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A study on the pot cultures of arbuscular mycorrhizal fungi in Korea (Arbuscular 내생균근 균의 포트배양에 관한 연구)

  • Lee, Snag-Sun;Eom, Ahn-Heum;Lee, Oun-Hack;Kim, Myoung-Kon
    • The Korean Journal of Mycology
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    • v.21 no.1
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    • pp.38-50
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    • 1993
  • Four plant (Sorghum bicolor, Cassia mimosoides var. nomame, Sesamum indicum and Glycine soja) were cultivated at the pots including the soils containing arbuscular mycorrhizal fungi and were also investigated with the colonizations and productions of arbuscular mycorrhizal fungi. Whereas the colonizations of arbuscular mycorrhizal fungi continuosly increased on the roots until 50 days, the productions of arbuscular mycorrhizal fungal spores were fluctuated with the terms of 30 days after inoculated. This indicated that the colonizations on the roots were not correlated with productions of arbuscular mycorrhizal fungal spores. Also, the various soils collected were applied to this technique by using pot cultures. Out of 82 various soils collected, the spore productions of arbuscular mycorrhiaze were observed only from 42 soils. The spores cultured under artificial conditions were identified to 15 species with four genera. The spore productions of arbuscular mycorrhizal fungi using this technique would be considered to be related to the soil pH: The spore productions were found in the low pH for the species of Acaulospora and Glomus, the those near pH 7.6 for the species of some Glomus, Scutellospora and Gigaspora.

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Factors Affecting Spore Formation of Carrot Leaf Blight Caused by Alternaria dauci In Vitro (배지에서 당근검은잎마름병균의 포자 형성에 영향을 미치는 요인)

  • Sena Yoon;Jiyoung Min;Heung Tae Kim
    • Research in Plant Disease
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    • v.29 no.3
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    • pp.251-257
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    • 2023
  • In order to examine the pathogenicity of Alternaria dauci, the causal agent of carrot leaf blight, it is necessary to standardize sporulation conditions to ensure the optimal quantity and quality of spore inoculum. Therefore, in this study, the effects of the growth medium, aeration treatment, and UV treatment with 12-hr photoperiod on the sporulation of A. dauci KACC42997 were investigated. A. dauci KACC42997 was pre-cultured for 7 days in a potato dextrose agar medium at 25℃ in the dark condition. When the pre-culture, after removing aerial mycelia, was re-incubated for 5 days, with simultaneous aeration treatment and 12-hr cycle UV treatment at 20℃, the largest number of spores was produced. One hundred seventy isolates of A. dauci were isolated from major carrot growing regions such as Pyeongchang, Gumi and Jeju and tested for sporulation under the conditions established in this study. Except for 20 strains, all strains produced spores. Statistically significant differences in the extent of sporulation were found among local populations of A. dauci isolates, but no difference was observed in their pathogenicity on carrots.

Supplemental knowledge on survival of Thelohanellus kitauei spores in vitro (In vitro에서 Thelohanellus kitauei 포자의 운명에 관한 지견보유)

  • 이재구
    • Parasites, Hosts and Diseases
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    • v.32 no.1
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    • pp.57-60
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    • 1994
  • In order to search the fate of Thelohonellus kitauei spores the extrusion rates of the polar filaments were monitored in uipo chronologically. Preserved spores suspended with various solutions at $-70^{\circ}C$ showed almost the same vigorous pattern as early freezing stages up to 1,750 days after initial preservation. It revealed that the vlabllltles of some spores suspended with 0.45% and 0.9% NaCl solutions and distilled water at $5^{\circ}C$ continued for 1,628 days, 1,614 days and 1,721 days, respectively. And, the life spans of some spores in the previous solutions added with antibiotics at $5^{\circ}C$ were 1,628 days, 1,614 days and 1,714 days, respectively. Key words: TheLohanellus kitnuei, spore, extrusion rate of polar filament, life span

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Sporulation or Cercospora canescens Ellis & Martin in culture (녹두 갈색무늬병균(Cercospora canescens Ellis & Martin)의 분생포자 형성에 관한 연구)

  • Kwon Shin Han;Oh Jeung Haing
    • Korean journal of applied entomology
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    • v.20 no.1 s.46
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    • pp.21-24
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    • 1981
  • This study was conducted to obtain a supply of conidia sufficient for screening mungbean mutant lines for a source of resistance to Cercospora leaf spot caused by Cercospora canescens Ellis and Martin. Abundant sporulation occurred in cultures on mungbean leaf decoction oatmeal agar(MOA) exposed to about 2,500 Lux of fluorescent light. but it did not occur in continuous darkness. The conditions that produced maximum number of conidia was not coincided with those for vegetative growth and pigmentation in culture medium. Removal of aerial mycelium in culture by brushing with sterile water so enhanced the conidial production that oatmeal agar medium(OA) could be useful for production of abundant conidia by the treatment.

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Epidemiological Studies of Rice Blast Disease Caused by Pyricularia oryzae Cavara I. Measurement of the Amount of Spores Released from a Single Lesion (벼 도열병의 역학적 연구 I. 단일병반으로부터 포자이탈량 조사)

  • Kim Chang Kyu;Yoshino Reiichi
    • Korean Journal Plant Pathology
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    • v.3 no.2
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    • pp.120-123
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    • 1987
  • Four types of spore trap (Kim's original, improved Kim's original, Yoshino's original and mixed type of Kim's and Yoshino's original) were evaluated for their efficacy to "estimate the amount of spores released from leaf blast lesions under the natural conditions. It was found that all four types had one or two defects in allowance for adequate sporulation/release, spore catch or spore counting. Thus, an improved type of spore trap was devised considering that it could cover the defects mentioned above. As a result, newly developed spore trap was quite satisfactory in above mentioned aspects and it could be used for pursuit of spore release phase under the natural conditions.

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Effect of Scrapping Aerial Mycelia and Light on the Production of Macroconidia and Chlamydospores of Cylindrocarpon destructans Causing Root Rot of Panax ginseng (기중균사 제거와 광처리가 인삼 뿌리썩음병균 Cylindrocarpon destructans의 대형분생포자 및 후막포자 생성에 미치는 영향)

  • Cho Dae-Hui;Yu Yun-Hyun;Ohh Seung-Hwan
    • Journal of Ginseng Research
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    • v.23 no.3 s.55
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    • pp.123-129
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    • 1999
  • Under the light condition of 25,000 Lux (12 hrs dark and light cycle) with scrapping treatment of aerial mycelia of Cylindrocarpon destructans on potato dextrose agar (PDA), V-8 juice agar, and ginseng extract agar, production of the macroconidia was increased to $3.7\~8.1$ fold over them produced in the dark. They were also produced $7.7\~18.0$ times more in the liquid cultures under the light condition than under the dark as well. PDA and V-8 juice agar among the tested were the best for the macroconidium production. On PDA, 1,585 $macroconidia/mm^2$ were produced under the light of 25,000 Lux with scrapping treatment of aerial mycelia of C. destructans, which is 3.2 and 1.4 times more than those produced under 3,000 and 10,000 Lux, respectively. Meanwhile, $20\~99$ macroconidia/$mm^2$ were produced by the non-scrapping under the light condition between 3,000 Lux and 25,000 Lux. The macroconidia were, however, lysed at $6\~7$ days after being incubated under the above range of the light. They were consisted of $1\~3$ cells in a macroconidium while $69.4\~100\%$ of them were the two-celled and the number did not seem to be affected by either the scrapping or the light. Production of chlamydospore converted from mycelia of C. destructans seemed to be promoted by the light and the scrapping as well. The 1,285 chlamydospres/$mm^2$ were produced with the light (25,000 Lux), which is 2.8 and 1.2 times more than those with 3,000 and 10,000 Lux, respectively. Scrapping the aerial mycelia of the cultures increased the chlamydospore formation to 1.9, 2.5 and 1.4 times more than the non-scrapping under the light intensity of 3,000 Lux, 10,000 Lux, and 25,000 Lux, respectively. On PDA, 1 to 8 chlamydospore(s) per catena were formed by all treatments tested and $34.2\~58.9\%$ of them was a single chlamydospore, However, the numbers was affected by neither the light ($3,000\~25,000$ Lux) nor the scrapping the aerial mycelia.

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Effect of Ultrasonic treatment on the Isolation of the Chlamydospores of Cylindrocarpon destructans Causing Root rot of Panax ginseng (초음파처리에 의한 인삼 뿌리썩음병균 Cylindrocarpon destructans 후막포자의 분리)

  • 조대휘;유연현
    • Journal of Ginseng Research
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    • v.24 no.2
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    • pp.53-57
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    • 2000
  • Chlamydospores were isolated from hyphae of Cylindrocanon destmctans by homogenization and/or ultrasonic treatment. Rate of the isolated chlamydospores by the homogenization with glass tissue grinder were 9.8% of all total chlamydospores formed in the culture of C. destructans. The length of mycelial fragments after the homogenization was about 400㎛ They were, however, formed in clusters of the chlamydospores and the mycelia The rate of the isolated chlamydospores from additional ultrasonic treatment after the homogenization of the mycelia were 74.3%. The length of mycelial fragments with the ultrasonic treatment was about 20 fm and chlamydospores seemed to be isolated from the mycelial mats and dispersed evenly in the culture. The numbers of chlamydospore in a catena were 1 to 8 cells after the homogenization on potato dextrose agar (PDA). Meanwhile the numbers of them after added ultrasonic treatment were 1 to 4 cells. Germination percentages of the isolated chlamydospores from the ultrasonic treatment were 46.8% after incubation of 2 days on PDA at 20。C and 60.7% after incubation of 13 days at 5。C, respectively. Germination rate of chlamydospores to the total chlamydospores produced by the ultrasonic treatment was 55.8%. However, it was increased to 74% when it was measured in the germinated catenae to the total catenae.

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젖산균의 열내성 포자에 관한 연구 (제1보) 포자의 열내성에 미치는 배양 조건의 영향

  • Yoo, Ju-Hyun;Choi, Kyu-Bong;Yoon, Won-Young;Lee, Jung-Chi;Kim, Tae-Han;Yang, Yung
    • Proceedings of the Korean Society for Applied Microbiology Conference
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    • 1975.12a
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    • pp.181.4-181
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    • 1975
  • 유포자 젖산균 Bacillus sp의 식품이용을 위한 기초로서 유포자 젖산균의 발육, 포자형성 등에 관하여 연구한 결과는 다음과 같다. 탄수화물 중 dextrin이 좋았고, Mn, Ca ion, biotin 등은 생육, 포자형성, 내열성에 대하여 효과가 좋았다. 배양의 최적조건은 호기조건하에서 $37~45^{\circ}C에서$ 36시간 이상 배양하는 것이었다.

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Relationship between excystment and abundance of HABs off the southern coast of Korea (남해안연안에서 적조생물 휴면포자 발아와 유영세포 출현과의 관계)

  • 이창규;임월애;정창수;조용철;김학균
    • Proceedings of the Korean Society of Fisheries Technology Conference
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    • 2000.05a
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    • pp.204-205
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    • 2000
  • 최근들어 우리나라 남해안연안은 내만역을 중심으로 편모조류에 의한 적조가 빈번히 발생하고 있는데, 이들 적조는 지역별, 시기별로 일정한 주기성을 보이면서 출현하고 있다. 이러한 적조의 시초는 무엇보다도 적조생물이 형성하는 휴면포자의 발아와 상관성이 있을 것으로 추정된다. 따라서 본 연구에서는 우리나라연안에서 출현하는 주요 적조원인생물 휴면포자의 발아률과 유영세포의 출현량을 조사함으로써 휴면포자의 발아와 유영세포 출현량과의 관련성 여부를 알아보았다. (중략)

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