• Journal of Wetlands Research
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• v.20 no.3
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• pp.272-280
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• 2018

Existing toxicity assessment researches were rarely studied on assessment of the abnormal behavior that affecting survival. Therefore, the study used anti-predator response of amphibians larvae, based on behavioral understanding, to assess toxicity in fullerene nanowhisker(FNW) and fullerene nanowhisker-silver nanoparticle composites(FNW-Ag). The experiment was conducted by dividing the groups not exposed to nanomaterials and exposed to nanomaterials at five concentrations(FNW: $10{\mu}g/ml$, FNW: $100{\mu}g/ml$, FNW: $500{\mu}g/ml$ FNW-Ag: $10{\mu}g/ml$, FNW: $50{\mu}g/ml$). As a result, there were no differences in normal activity except those exposed to concentrations of FNW-Ag $50{\mu}g/ml$, but there were statistically significant differences in anti-predator response except those exposed to concentrations of FNW $10{\mu}g/ml$. That is, exposed of nanomaterials does not affect ordinary movements, but analyses based on behavioral understandings have shown that it has an influence on the anti-predator response. It is therefore considered necessary to have animal behavioral analysis method performed in the assessment of eco-toxic experiment.

• Journal of Wetlands Research
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• v.17 no.4
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• pp.407-413
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• 2015

As the number of applications containing nanomaterials increase, aquatic ecosystem exposure to nanoparticles (NPs) is unavoidable. In this study, we carried out toxicity assessment to Au-nanoparticles(NPs) of Rana dybowskii eggs and tadpoles. Toxicity was recorded hatching rate, body condition(Snout-tail length, STL), and behavioral sensitivity. Behavioral sensitivity was analyzed to anti-predator behavior using Ethovision XT 9. Au-NPs did not show any toxicity of hatching rate and STL. But, Tadpoles exposed to Au-NPs decrease behavioral sensitivity of stimuli. This study has value of environmental toxicity evaluation because these results show the new way of toxicity assessment.