• KOREAN POULTRY JOURNAL
• /
• v.36 no.8 s.418
• /
• pp.70-74
• /
• 2004

계사환경을 쾌적하게 하고 생산성을 향상시키고자 하는 것은 모든 양계인들의 바람이다. 특히, 요즘과 같은 여름철의 경우 고온 다습한 기후로 인해 계분처리가 잘 이루어지지 않을 경우 계사내 악취방생으로 인해 계사환경이 나빠질 수 있으며, 겨울철이나 환절기에는 환기부족에 따른 가스발생으로 닭들의 생산성 저하를 가져오기도 한다. 즉, 계분에 따른 악취발생은 호흡기 질환의 원인이 되고, 악취스트레스, 소화불량, 사료섭취 저하 등으로 인해 폐사율이 증가하여 결국 생산성저하 현상이 높게 나타날 수 있다. 따라서 최근 축산환경개선이라는 포괄적인 문제를 해결하기 위하여 관련업계에서 다양한 실험과 연구결과가 속속 발표되고 있으며, 양계분야에서도 환경개선은 물론 생산성 향상을 기할 수 있는 대체 물질이 개발, 사용되어지면서 업계의 비상한 관심을 모으고 있다. 본지가 찾은 곳은 자연미생물을 이용해 계분의 악취를 제거하고 생산성 향상을 기하고 있는 농가(일심농장)와 공급업체인 운석바이오(대표 이종수)를 찾아 국내 축산환경개선 방안을 점거해 보고자 한다.

• Proceedings of the KSAR Conference
• /
• 2001.03a
• /
• pp.14-14
• /
• 2001

본 연구는 생명공학 관련 기술개발에 의하여 신소재 물질을 생산하고 사람에게 안전하고 생리활성이 높은 고가의 의료용 단백질 생산기술을 체계화하며 형질전환 가축을 이용한 유용물질 생산에 따른 산업화와 부가가치의 극대화에 그 목적이 있다. 유즙으로 사람의 빈혈치료제인 EPO(Erythropoietin)를 분비할 수 있는 형질전환돼지를 생산하기 위하여 WAP(Whey Acidic Protein) Promoter(2.6kb) 하류에 사람의 조혈촉진유전자(EPO: 2.6kb)를 연결시켜 미세주입용 재조합 벡터(WAP-EPO : 약 7.8kb)를 구축하였다. 구축된 재조합 벡터를 1세포기 수정란에 약 2ng/ul 농도로 미세주입한 다음 외과적 방법으로 이식하였다. 이식 후 분만 모돈으로부터 생산된 자돈의 꼬리조직을 이용 게놈DNA를 추출하고 PCR 검정을 한 결과, 유즙으로 사람의 빈혈치료제를 생산할 수 있는 유전자가 도입된 형질전환돼지 $\boxDr$새롬이$\boxUl$ (♂)를 확인하였다. 또한 이렇게 꼬리조직으로부터 확인된 새롬이의 혈액과 정액을 채취, 게놈 DNA를 추출하여 외래 유전자 삽입여부를 PCR 방법으로 검정한 결과 꼬리조직과 마찬가지로 혈액 및 정액에서도 외래유전자가 삽입되었음을 확인할 수 있었다. 이렇게 생산된 형질전환돼지 $\boxDr$새롬이$\boxUl$를 이용 계대번식을 통한 F$_1$ 산자의 생산과 유전자 전이율을 확인하기 위하여 $\boxDr$새롬이$\boxUl$정액을 이용한 인공수정을 실시하였다. 인공수정은 1999년 7월 1일부터 2000년 9월 8일 까지 총 78두의 모돈을 이용하였으며, 그 중 21두의 모돈이 분만하여 인공수정에 의한 분만율은 26.92%로 나타났다.(Table Omitted) Table 1에서와 같이 새롬이 정액을 이용한 인공수정에 의해 형질전환된 F$_1$ 자돈의 형질전환율은 17.98%로 나타났으며, 32두의 형질전환자돈 중 8두(암:4두, 수:4두)는 분만과 동시에 폐사하였거나 사육중 폐사하여 현재 24두(암:12두, 수:12두)가 생존하여 F$_1$ 간 교배계획에 의해 사육되고 있다. 이 중 암컷 4두는 현재 F$_2$ 자돈 생산과 함께 유즙내로 사람 빈혈치료제의 분비 유무를 검정중에 있으며, FISH 법에 의한 외래 유전자 삽입 검정을 확인 중에 있다.

• Journal of fish pathology
• /
• v.25 no.1
• /
• pp.47-58
• /
• 2012

Behavioral and physiological responses of juvenile red seabream (Pagrus major) to different concentrations of ethanol were investigated. No swimming and no reaction to touching by a wooden stick was observed at 0.6% ethanol group in behavioral response, and survival rate was 100% after 5 hours of treatment. Red blood cell count, hematocrit and hemoglobin levels in plasma were not significantly different among all groups. AST activities in plasma significantly decreased as ethanol concentration increased. On the contrary, ALT activities in plasma significantly increased as ethanol concentration increased. Cortisol level in plasma was the lowest in 0.6% ethanol group. Glucose levels in plasma increased significantly when ethanol concentration increased more than 0.4%. Oxygen consumption of fish in 0.6% ethanol seawater was constantly lower than that of fish in control seawater from 2 hours after the exposure to ethanol seawater until the end of experiment.

• Journal of Aquaculture
• /
• v.11 no.4
• /
• pp.437-447
• /
• 1998

In mass production of larval rockfish, Sebastes schlegeli, high mortality had been observed frequently. This may be the consequence of the low nutritional quality of the live feeds used. This experiment was designed to find out a suitable diet for the mass production of rockfish larvae. Three kinds of live feeds were tested ; plutei of sea urchin (Hemicentrotus pulcherrimus), L-type rotifers (Brachionus plicatiilis) and Artemia naupii. The latter two were enriched with ${\omega}$-yeast, Spirulina platensis and Super Selco before feeding to rockfish larvae. The sea urchin plutei caused to poor survival and growth rates for larval rockfish, and therefore, they were not seemed as proper feed for rockfish larvae. Enrichments of rotifers and Artemia nauplii with ${\omega}$-yeast, Spirulina platensis, or Super Selco improved survival and growth rate. But, rotifers enriched with Super Selco resulted in better rockfish larvae survival than those enriched with ${\omega}$-yeast. A sudden increase of mortality occurred around 11 days after birth. In this critical period, a shift feed such as Artemia nauplii had been supplemented with rotifers. After this critical transition period, the moratality gradually decreased by feeding Artemia nauplii enriched with PUFA. Feeding of mixed feed with rotifers and Artemia nauplii resulted in better larval survival and growth than those of each live food alone.

• Korean Journal of Poultry Science
• /
• v.18 no.3
• /
• pp.167-181
• /
• 1991

In order to study the effects of dietary yeast (Saccharomyces cerevisiae) products on the performance of broiler chickens, tow feeding trial using Arbor Acres strain were conducted. In experiment 1, 200 hatched male broiler chickens were divided into groups of 10 birds each and four groups were given each of the five dietary treatments : control, 0.25% yeast culture supplemented (YC-0.25%), 2.5% yeast culture supplemented(YC-2.5%), 0.05% live yeast supplemented(LY-0.05%) and 0.1% live yeast supplemented(LY-0.1%) . In experiment 2, 240 hatched broiler chickens(120 birds in each sex) were assigned to 2$\times$3(sex$\times$feed) factorial design Dietary treatments were control, 0.1% live yeast supplementation in finisher diet(LY-Finisher) , and 0.1% live yeast supplementation in whole period (LY-Whole). Results of experiment 1 showed that weight gain, feed intake and mortality were not significantly different among treatments. However, weight .gain of YC-0.25% and LY-0.1% tended to be greater than other treatments after 3wks of age Feed efficiency of LY-0.05% was poorer than those of control, YC-0.25% and LY-0.1% . Although nutrients availabilities were not significantly different among treatments, availiabilities of Ca and P were greater in yeast products supplemented groups than in control group. The number of Lactobacillus spp., Streptococcus spp . and yeast in small intestine tended to be greater in supplemented groups while that of Coliforms bacteria tended to be greafter in control group. In experiment 2, there were significant effects of factors (feed and sex) and interaction on growth rate. LY-Whole groups showed best weight gain in male while LY-Finisher groups did best in female broiler chickens. Feed intake and mortality were significantly higher in male broiler chickens. Feed$\times$sex interaction had a significant effect on feed efficiency. LY-Whole groups showed best feed efficiency in male while LY-Finisher did best in female broiler chickens. Availabilities of dry matter, Ca and P were higher in male than in female broiler chickens. Availabilities of Ca and P were higher in live yeast supplemented groups than in control groups. Live yeast supplemented groups tended to have greater number of Lactobacillus spp . and yeast in the small intestine and Stre))tococcu spp. in the small intestine and cecum, and lesser number of Coliforms bacteria in the small intestine The pH of small intestinal contents tended to be higher in live yeast supplemented groups.

• KOREAN POULTRY JOURNAL
• /
• v.13 s.146
• /
• pp.108-116
• /
• 1981

제15회 산란계 경제능력검정에는 총 13개 계군이 출품되었으며 능력검정 결과를 요약하면 다음과 같다. 1. 육추율, 육성율, 성계생존율 이번회 검정계의 육추율은 13개 계군 평균 97.72$\%$로서 14회 99.7$\%$와 비슷하다. 특히 이번 검정에서 육추기간 중 한마리의 폐사도 없는 구는 3구였으며 9구 만이 79.64$\%$로 육추율이 가장 낮았는데 그 원인은 1개 검정소에서 육추기간 중 백혈병으로 인한 폐사수가 많았기 때문으로 보인다. 육성율 평균은 99.07$\%$로서 14회 99.0$\%$와 같았고 한마리의 폐사도 없는 구는 11구로서 최고 육성율을 보였다. 성계 생존율은 평균이 90.14$\%$로서 14회의 92.6$\%$에 비슷 하였으며 13구는 97.98$\%$로서 상당히 좋은 성적을 보였으며 6구, 7구 9구, 10구 및 11구는 90$\%$이하로 성계생존율은 5회 점검 이후 점차 높아 졌는데 이유는 MD백신이 개발되어 이의 접종으로 인한 마렉병의 발생을 예방 할 수 있었던 것으로 생각된다. 2. 성 성숙일령 15회 검정에서 13개 계군의 평균 성 성숙 일령은 163일에서 14회 161일과는 비슷하였는데 성 성숙 일령은 육성기의 사료 급여와 사양관리에 따라서 영향을 많이 받는 형질이므로 더 이상의 고찰이 필요하지 않다. 성 성숙이 제일 빠른구는 5구의 154일이었고 늦은 구는 9구의 168일 이었는데 그 차는 14일이였다. 3. 산란율 및 산란지수 이번 검정계군의 평균 산란율은 70.14$\%$로서 14회에 73.8$\%$와 약 3.6$\%$가 낮았다. 한편 최고 산란율을 나타낸 구는 5구의 73.86$\%$였으며 산란율이 제일 낮은 구는 11구의 65.95$\%$였다. 산란지수의 평균은 232.02로서 14회의 246.5보다는 14.5개가 적었는데 이는 산란율에서와 같은 경향치 였다. 최고 산란 지수를 보인 구는 5구의 250.69였고 2구와 3구, 8구, 13구도 240이상으로서 상당이 좋은 성적을 보였으며 9구는 204.82로서 산란지수가 최하위 였고 최상위와 최하위와의 산란지수의 차이는 무려 45.87이었다. 4. 사료 요구율 13개 계군의 평균사료 요구율은 2.67로서 14회 2.68과 비슷하였다. 사료 요구율이 제일 낮았던구는 3구의 2.56이였고 사료 요구율이 제일 높았던 구는 9구의 2.78이였다. 5. 난중 13개 계군의 평균난중은 60.72g으로서 14회 59.08g보다는 약간 무거웠는데 난중은 다른 형질에 비하여 유전력이 높은 형질인 것을 감안할 때 사양관리 면에서의 영향이 아니고 출품계군의 구성에 의한 영향인 것으로 생각된다. 8회 검정 성적 중 난중이 제일 무거운 구는 4구의 63.32g이었고 제일 가벼운 구는 5구의 58.08g이었다. 6. 체중 300일령 평균체중은 1,966.33.g으로 14회 1,986.7g보다는 가벼웠는데 체중 역시 유전력이 높은 형질로서 이 차이는 검정계군의 구성에 따른 차이로 생각 된다. 500일령 체중의 평균은 1,985.68g으로 14회의 1,994.9g보다는 약간 가벼웠으나 500일령 체중에 있어서와 같이 검정계군의 구성에 따른 차이로 생각 된다. 7. 사료 섭취량 육추기간 중의 1일 1수당 평균사료 섭취량은 32.97g으로 14회 34.82g보다 적었는데 이것 역시 출품계군의 구성에 있어 겸용종 내지 유색종의 비율이 낮았던 데에 기인한 것으로 생각된다. 육성기의 1일 1수당 사료섭취량은 70.06g으로서 14회의 70.91g과 같았고 산란기간 중의 1일 1수당 사료섭취량은 평균이 113,54g으로서 14회 115.81g과 비교할 때 큰 차가 없었으며 특히 11구는 118.61g으로 가장 사료를 많이 섭취 했는데 산란기 수당 사료 섭취량은 해가 거듭될수록 적어지는 경향이었다. 8. 경제성 13개 검정계군이 총 수입은 45,405원으로 계란 판매 수입은 86.4$\%$였고 폐계 판매수입은 13.59$\%$였으며 총 지출은 33,657원으로 사료비의 지출비율이 95.5$\%$였고 초생추대가 4.5$\%$였다.

• Journal of Sericultural and Entomological Science
• /
• v.29 no.2
• /
• pp.51-57
• /
• 1987

The intent of this research is to acquire some basic informations about formulation of the viral pesticide, Hyphantria cunea nuclear polyhedrosis virus and its virulence under field condition. The nuclear polyhedrosis virus was formulated as wettable powder using spreader, sticker and U.V. protector. The formulated product and aqueous virus were diluted with water at the concentration of 1${\times}$106PIB/ml and sprayed on mulberry leaves in the field. The leaves were fed with 3rd instar larvae of H. cunea to determine the inactivation period of the viral pesticides. The aqueous virus was completely inactivated on 5th day after spray, while the formulated one showed a spare mortality to the larvae even on 20th day after spray. In field application test, The fromulated and aqueous virus were sprayed on individual mulberry tree and 3rd instar laevae of H. cunea were fed on the trees. The mortality of the larvae one day after spray of the formulated and aqueous virus were about 50% and 40%, respectively. The formulated virus exhibited a persistent virulence to the larvae up to 9th day after spray, which the mortality was approximately 30%. The residual virulence of the formulated and aqueous virus was extended up to 4th day and 2nd day after spray, respectively.

• Korean Journal of Poultry Science
• /
• v.41 no.3
• /
• pp.197-204
• /
• 2014

The objective of this study was to evaluate the effects of different levels of red ginseng mare or Korean mistletoe powder on growth performance and physicochemical properties of thigh meat in broiler. A total of 200 broiler chicks were divided into five groups were fed a basal diet (control), basal diet supplemented with 0.5% red ginseng mare (T1), red ginseng mare 1% added (T2), Korean mistletoe 0.5% added (T3), Korean mistletoe 0.5% added (T4) for five weeks. Growth performance and proximate composition of chicken thigh meat were not influenced by all treatments, except for mortality and crude fat, which was significantly lower in all treatments with red ginseng mare or Korean mistletoe powder compared to control. Dietary red ginseng mare and Korean mistletoe supplementation had no significantly effects on pH, WHC, shear force, CIE $L^*$, $b^*$ values, triglyceride and glucose but TBARS values total cholesterol, and LDL-cholesterol were decreased (P<0.05) by the addition red ginseng mare and Korean mistletoe powder. CIE $a^*$ values increased significantly with added red ginseng mare and Korean mistletoe powder relative to the control (P<0.05). It was concluded that the addition of red ginseng mare and Korean mistletoe powder revealed minor improvements in the lipid oxidative stability of chicken meat.

• Korean Journal of Poultry Science
• /
• v.33 no.2
• /
• pp.159-164
• /
• 2006

To investigate the effects of salt contents in diet and water on the growth of broiler chicks, 288 heads of Hubbards strain chicks, were fed with com-soybean meal diets containing 0, 0.25, 0.5, 0.75 1.0, 2.0, 3.0, or 4.0% salt from 1d to l4d posthatching. And 180 chicks were also supplemented with salt to their water at 0, 0.1, 0.2, 0.5 or 1.0%. Feed intake and weight gain tended to decrease as the salt levels in diets increased and those were the lowest in 4% group as 481 g and 168 g, however, mortality was the highest in 4% group as 75.0%. As for the salt levels in water, feed intake and weight gain were low as 427 g and 162 g in 1% group, while mortality was 77.8%. Salt poisoning chicks were shown with thirsty, appetite deficiency, rising impossibility, sleepiness, or convulsions, and in autopsy, were shown with ascites, hydropericardium, cardiac hypertrophy, hemorrhage of eyelid, hemorrhage of trachea, hemorrhage of intestine, etc. Conclusively, salt content above 4% in diet or 1% in water could reduce the performance in broiler chicks.

• Journal of fish pathology
• /
• v.27 no.1
• /
• pp.17-24
• /
• 2014

To study the possible use of probiotics in fish farming, The in vitro and in vivo antibacterial effects of Pseudomonas aeruginosa MB I-3 (MB I-3) against the fish pathogenic bacterium Listonella anguillarum were evaluated. The inhibitory effects of MB I-3 against vibrios were investigated by the double layer method and the co-culture. The results showed that MB I-3 inhibited the growth of pathogenic vibrios including Listonella anguillarum, Vibrio alginolyticus, Vibrio cholerae, Vibrio fluvialis, Vibrio furnissii, Vibrio harveyi, Vibrio parahaemolyticus and Vibrio vulnificus. Extracellular substances obtained from the cultural supernatant of MB I-3 by ethyl acetate extraction showed inhibitory effects on L. anguillarum. The antibacterial substance of MB I-3 was evaluated to destroy the cell membrane of L. anguillarum in electron micrographs. The probiotic effects of MB I-3 was tested by exposing olive flounder (Paralichthys olivaceus) fry to L. anguillarum with or without MB I-3. The cumulative mortality of olive flounder fry infected with L. anguillarum was 24% in the group with MB I-3, while it was 46% in the control group without MB I-3. These results indicate that MB I-3 has potential applications as a probiotic for the control of fish pathogenic vibrios in fish rearing system.