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Rapid Agrobacterium-mediated genetic rice transformation method using liquid media (액체배양을 이용한 단기 벼 형질전환 방법)

  • Yang, Dae-Hwa;Chang, Ahn-Cheol;Ahn, Il-Pyung;Kim, Hae-Jung;Kim, Dong-Hern;Lee, Hyo-Yeon;Suh, Seok Cheol
    • Journal of Plant Biotechnology
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    • v.40 no.1
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    • pp.37-42
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    • 2013
  • Rice is one of the most important cereal crops as a model plant for functional genomics of monocotyledons and usually transformed using Agrobacterium tumefaciens. However, the transformation's process using previous method is still time consuming and uneconomical, low efficiency. In this study, we established a new method by modifying the general Agrobacterium protocol especially in the infection and co-cultivation, Agrobacterium elimination, infected calli's selection steps using liquid media. We directly inoculated Agrobacterium containing a ZjLsL gene under the control of constitutive promoter into the 1- to 3-week-old rice calli derived from mature seeds. After 3 days of co-cultivation, the infected calli were transferred onto liquid media of Agrobacterium elimination and calli's selection for 3 days. The calli were transferred to calli's growth solid media for 14 days and then the calli transferred to shoot induction and root induction media. Putative transformants were initially selected on the medium containing phosphinothricin, and the PAT protein verified by PAT strip test. This method in this study would lead to reduction of substantial labor and time to generate transgenic plants.

Micromorphological Observation of Honam Series -Some characteristics of clay films in B horizon- (호남통(湖南統)에 대(對)한 토양(土壤) 미세형태학적연구(微細形態學的硏究) - B층(層)의 점토피막(粘土皮膜) 특성(特性)에 관(關)하여 -)

  • Shin, Jae Sung;Um, Ki Tae;Shin, Yong Hwa
    • Korean Journal of Soil Science and Fertilizer
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    • v.7 no.2
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    • pp.65-70
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    • 1974
  • Micromorphological features of Bhorizon of Honam series were investigated by means of thin sections of natural undisturbed, oriented soil samples. Thin sections show that the B horizons have argillic accumulations. Argillic horizon was developed at the utmost in B21t horizon. Channel, and skew plane cutans were identified in B21t horizon and stress cutans are presented in B24t horizon. Such a continuous oriented argillans related to channels and skew planes decreased with increasing depth.

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A Study on the Coefficient of Linear Extensibility of various Paddy Soils in Korea (우리나라 수종(数種) 답토양(畓土壤)의 선형팽창(線型膨脹) 지수(指数)에 관(關)한 연구(硏究))

  • Jung, Yeun-Tae;Ha, Ho-Sung
    • Korean Journal of Soil Science and Fertilizer
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    • v.16 no.1
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    • pp.1-6
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    • 1983
  • The results of COLE(Coefficient of Linear Extensibility) measurement on various paddy soils in Korea are summarized as follows; 1. The COLE values of paddy soil in Korea varied from 0.024 to 0.094 in the surface soil while in the sub-surface soils those were ranging from 0.022 to 0.115. The poorer the relative drainage and the finer the texture caused, the greater the COLE values. 2. The matured clayey soils on fluvio-marine plains and local alluvium derived from the Tertiary materials have COLE values more than 0.09 which is demonstrated that the necessity of COLE measurement throughout profiles so that could be considered the characteristic in the characteristic of those soils. 3. The clay content has the highest positive correlation (r=0.81~0.76) values. The content of organic matter, water content at 1/3 bar, Atterberg limits, water stable aggregate etc. also have significant positive correlation with COLE values while the context of sand and silt show negative correlation. 4. Although the COLE values measured on horizontal linear bases were slightly greater than those measured on volume bases practicability was for granted. For more accurate measurement of COLE, it is reasonable to have the average values of COLE calculated from the horizontal and vertical bases.

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Soil Microbial Diversity of Paddy Fields in Korea (논 토양 서식 미생물의 다양성에 관한 연구)

  • Suh, Jang-Sun;Shin, Jae-Sung
    • Korean Journal of Soil Science and Fertilizer
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    • v.30 no.2
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    • pp.200-207
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    • 1997
  • In order to evaluate the soil microbiological characteristics of paddy fields in Korea, surface soils were sampled from 63 sites in different agroclimatic zones before submersion of the fields. The distribution of microorganisms and the microbial diversity indices were examined. Soil microbial populations were generally higher in southern area than in northern area. The colony forming units(cfus) of fluorescence Pseudomonas sp. showed the greatest regional differences, among the microbes investigated. On the topographical differences, the cfus of aerobic bacteria, fluorescence Pseudomonas sp. and Azotobacter sp. maintained high level in coastal plains; and on the sail textural difference, fungus was the highest in clay soil, but Bacillus sp., Azotobacter sp and denitrifiers were the highest in silty clay loam soil at 0.05 probability level based on the multiple range test. The numbers of ammonium oxidizers and Azotobacter sp. were increased with soil pH. Microbial diversity indices of paddy fields which calculated from the percentages of Bacillus sp. fluorescence Pseudomonas sp. Azotobacter sp. denitrifiers, ammonium oxidizers, nitrite oxidizers, actinomycetes and fungus to these total microbial numbers were between 0.109 and 0.661. On the soil textures, the microbial diversity indices of sandy, sandy loam, silty clay loam, clay loam and clay soil were 0.443, 0.427, 0.414, 0.405 and 0.362 respectively.

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압축성을 고려한 캘린더 닙 내에서의 열전달 현상에 대한 해석

  • 이학래;박선규
    • Proceedings of the Korea Technical Association of the Pulp and Paper Industry Conference
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    • 2000.11a
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    • pp.117-117
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    • 2000
  • 제지공정의 마지막 단계인 캘린더는 지필의 표면을 평활하게 하고 두께를 감소시켜 균일 하게 하는 역할을 한다. 하지만 캘린더링은 인장강도 둥의 강도적 성질과 불투명도 등 광학 적 성질을 저하시키는 공정이기도 하다. 따라서 캘린더령 공정에 의한 제품 품질의 저하를 극소화하기 위해서는 공정인자의 엄밀한 관리가 요청된다. 캘린더링의 주요 공정인자에는 온도와 압력, 닙 체류시간, 유입지의 함수율, 유입지의 온도 롤의 재질 등이 있다. 이 가운 데 특히 캘린더링 온도와 압력은 주요한 공정 인자이다. 따라서 이들 변수에 의한 캘린더링 공정의 변화를 정확하게 파악하는 것은 매우 중요하다. 캘린더링 공정의 속도와 관련된 닙 체류시간과 유입지의 함수율, 유입지의 온도, 롤의 재질 등은 실제 공정에서 변화시키기 어 려운 반면 온도와 압력은 조절이 비교적 용이한 특정도 지니고 있다. 캘린더링 전후의 종이의 물성 차이는 지필 내부로의 열 침투에 의한 열변형에 따라 크게 달라진다. 셀룰로오스는 유리전이온도 이상으로 가열되면 그 성질이 크게 변화하므로 캘린 더링 시의 온도가 유리전이온도보다 높거나 낮은 경우 캘린더링된 종이의 물성 차이가 크게 달라질 수 밖에 없다. 캘린더령은 비정상상태에서 진행되기 때문에 지펼의 내층보다는 표층 으로부터 순차적으로 열변형이 발생하는 공정이다. 그러므로 지필의 두께 방향으로의 열 침 투 현상의 해석을 통하여 캘린더링 시 유리전이온도가 어느 깊이까지 도달하는가를 파악하 는 것은 캘린더링 공정의 해석에 매우 중요하다. 캘린더링 공정에서 발생하는 열전달현상 해석 시 지필의 압축을 고려하지 않고 비압축성 물질로 가정하는 것은 캘린더링 공정 인자 중 압력에 의한 영향을 제대로 평가하지 못하는 한계를 지니게 된다. 따라서 본 연구에서는 지펼의 압축성을 고려하여 캘린더링 모델을 정립 하고, 이를 토대로 캘린더링 공정 조건에 따른 열 침투 현상을 해석코자 하였으며, 그 방법으 로 수치해석기법을 도입하였다. 또 실제 캘린더링 전후의 두께 변화를 측정하여 유리전이온도 의 도달 깊이와 비교하였다. 지필의 압축 정도는 롤의 직경과 닙 폭을 이용하여 MD 방향으 로 함수화하였으며, 열전달 계수로는 겉보기 값을 사용하였다. 이때 지펼은 균질한 것으로 가 정하였다. 함수율은 유리전이온도를 좌우하는 가장 큰 인자이나 본 연구에서는 항온항습처리 를 통해 유입지의 함수율을 고정시켰으며 캘린더링 시 함수율의 변이는 없다고 가정하였다. 그 결과 열침투깊이가 증가할수록 지필은 보다 변형되기 쉬운 상태가 되어 주어진 압력 조건에 대해 소성변형 정도가 증가하는 것으로 나타났다. 이는 캘린더링 전후에 두께 변화를 측정하여 정량적으로 평가할 수 있었다. 수치해석기법을 통해 같은 압력 조건에서 온도가 증 가함에 따라 혹은 같은 온도 조건에서 압력이 증가함에 따라 지필 내의 유리전이온도의 침투 깊이가 증가함을 알 수 있었으며 이는 캘린더링 전후의 두께 변화의 측정 결과와 일치하였 다. 또 NRT가 증가함에 따라서도 유리전이온도 침투 깊이가 증가하였다.

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Comparative Analysis of Forest Fire Danger Rating on Forest Characteristics of Thinning Area and Non-thinning Area on Forest Fire Burnt Area (산불적지에서 숲 가꾸기 실행 유무와 산불위험성 비교 분석)

  • Lee, Si-Young;Lee, Myung-Woog;Yeom, Chan-Ho;Kwon, Chun-Geun;Lee, Hae-Pyeong
    • Fire Science and Engineering
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    • v.23 no.4
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    • pp.32-39
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    • 2009
  • In this study, 18 plots were selected for particular investigation on Gangneung area and Samcheok area and Uljin area, where forest fire was occurred after thinning in 2007. After selection, a relationship between a damage of forest fire and thinning were compared and analyzed. Many factor such as a damaged species, a thinning or non-thinning, a direction of forest fire head, DBH, a height of tree, a mortality of tree, a leeward scorching ratio, a crown damage ratio, a forest tree standing crop density, a crown base height, a leaving of thinning slash, a location of plot (GPS), elevation, an aspect of slope, an angle of slope, and a topography were measured. Therefore, we analyzed a relationship with forest fire. In the result of this analysis, it was founded that the leeward scorching ratio was 24.7% on thinning area whereas 60.2% on non-thinning area. Subsequently, leeward scorching ratio on thinning area was increased by about 35.5% more than on non-thinning area. In the result of analysis about tree species, a forest of pine tree was more damaged than forest of oak tree. The mortality of tree was increased by about 41.4% on non-thinning area. When stands were close to thinning slashes, these were more damaged by 10${\sim}$20% than other stands for increasing of amount of a combustion material. Especially, as potential of re-ignition increased for more cumulated thinning slash, it will be more important to require a method for a periodical removing or practical utilization of slash.

Preparation TiO$_2$sol using aqueous system and characteristics of its thin film (수용계를 이용한 TiO$_2$Sol의 제조와 박막의 특성)

  • 김성도;조경식;김성진
    • Journal of the Korean Crystal Growth and Crystal Technology
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    • v.10 no.3
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    • pp.271-277
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    • 2000
  • Transparent $TiO_2$ thin films were prepared by the sol-gel method from titanium alkoxide aqueous solution containing large quantities of water. To prepare the aqueous system sol, the chelate compound solution were prepared from each 1 mole of titanium(IV)iso-propoxide, acetylacetone and 8 moles of isopropyl-alcohol. And then the 50 moles aqueous solution with the 0.02~0.50 moles of HCI for sol stability and the 0.3 mole of polyethylene glycol for coating adhesion were precisely dropped on the chelate compound solution. $TiO_2$thin films were fabricated by dip coating, drying and heat treatment at $400^{\circ}C$ and their characteristics were investigated by XRD, SEM and UV-visible spectrometer, The neutral sol of composition with TTIP : AcAc : IPA : $H_2O$ : PEG : HCl = 1 : 1 : 8 : 50 : 0.3 : 0.15 (molar ratio) was maintained stable sol condition and without problem for coating more than 30 days. The anatase phase $TiO_2$thin film prepared from 30 times dipping, drying and heat treating showed the flat surface and the fine microstructure of fracture surface with about 2 $\mu\textrm{m}$ thickness. Transparency of these $TiO_2$thin film was 80 % in the visible region.

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High Performance Object Recognition with Application of the Size and Rotational Invariant Feature of the Fourier Descriptor to the 3D Information of Edges (푸리에 표현자의 크기와 회전 불변 특징을 에지에 대한 3차원 정보에 응용한 고효율의 물체 인식)

  • Wang, Shi;Chen, Hongxin;I, Jun-Ho;Lin, Haiping;Kim, Hyong-Suk;Kim, Jong-Man
    • Journal of the Institute of Electronics Engineers of Korea CI
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    • v.45 no.6
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    • pp.170-178
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    • 2008
  • A high performance object recognition algorithm using Fourier description of the 3D information of the objects is proposed. Object boundaries contain sufficient information for recognition in most of objects. However, it is not well utilized as the key solution of the object recognition since obtaining the accurate boundary information is not easy. Also, object boundaries vary highly depending on the size or orientation of object. The proposed object recognition algorithm is based on 1) the accurate object boundaries extracted from the 3D shape which is obtained by the laser scan device, and 2) reduction of the required database using the size and rotational invariant feature of the Fourier Descriptor. Such Fourier information is compared with the database and the recognition is done by selecting the best matching object. The experiments have been done on the rich database of MPEG 7 Part B.

The Distribution and Characteristics of Geographical Names on 1:50,000 Topographic Map of the Korean Peninsula in the Early 20th Century ("오만분일지형도(五萬分一地形圖)"에 나타난 20세기 초 한반도의 지명 분포와 특성)

  • Kim, Sun-Hee
    • Journal of the Korean Geographical Society
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    • v.43 no.1
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    • pp.87-103
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    • 2008
  • The geographical name is an expression of human knowledge about living conditions and a basic tool for understanding about regional cultures and history as a result of spatiotemporal changes. This study aims to understand the historical and regional characteristics by analyzing the frequency and distribution of geographical names of 1:50,000 topographic map in the early 20th century and materializing on electronic cultural atlas. The result of this analysis is as follows. First, the aspect of distributed geographical names on the Korean Peninsula reflects a general trait of the country's natural and human environment included geographical features, population, arable land, the number of counties and villages, and functions of administration and military, etc. Second, through the frequency and weight of their names, the analysis shows not only the change of native names, but also the country's phase of the time by Japanese colonial policies such as exploitation of resources, the construction of railroads, and a desire to control of the border area with Manchuria. In addition, the study identified regional characteristics and differences in environmental perception and preferences, and naming basis and forms by the comparative analysis of each type of geographical names associated with village and ridge such as 'Chi' 'Ryung' 'Duk' 'Hang' 'Pyeong' 'Gok'. In particular, those characteristics were largely affected from environmental difference of each area.

A STUDY ON THE RELATIVE SHEAR BOND STRENGTHS OF SOME ADHESIVE RESTORATIVE MATERIALS TO PRIMARY ENAMEL AND DENTIN (수종 접착성 수복재의 유치 법랑질과 상아질에 대한 상대적 접착력의 비교연구)

  • Kim, Seung-Mee;Kim, Shin;Jeong, Tae-Sung
    • Journal of the korean academy of Pediatric Dentistry
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    • v.27 no.2
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    • pp.237-245
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    • 2000
  • For the purpose of comparing the bond strengths of some tooth adhesive restoration materials on primary enamel and dentin, 4 kinds (7 brands) of restorative materials including a composite resin (Z 100), a conventional glass ionomer cement (Chem-Flex), 2 brands of resin-modified glass ionomer cements (Fuji II LC-I, Vitremer), and 3 brands of compomers(Dyract AP, F2000, Compoglass) were investigated using UTM for measuring the shear bond strengths. Additionally the failure modes were examined by histologically observing the fractured surfaces of each specimen. The following results were obtained. 1. The shear bond strengths of Z 100 to the primary enamel were higher than those of other experimental materials except Fuji II LC-I, which showed significantly higher bond strength than Chem-Flex or Vitremer (P<0.05). 2. The shear bond strengths of Z 100 to the primary dentin were higher than those of other experimental materials except Dyract AP and Fuji II LC-I, both of which showed significantly higher shear strength than Chem-Flex or Vitremer (P<0.05). 3. The shear bond strengths of all restorative materials except Dyract AP showed relatively higher values to enamel surface than to dentin surface. In Dyract AP, the reverse was true significantly. 4. All materials examined showed cohesive failures except some Chem-Flex and Vitremer, which showed adhesive failures.

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